Status
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Gene information
General information
TC009794 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 8203090..8211634
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009794
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009794 RA
      MSRESLNEV LEKIAQVTA ILVETSKIK EQLDRSASD 
      EKLQVIIEQ TEVQVNSLV KHATSVKEL FDKNMKTSE 
      KLLVNGVDI NKNSETNGN DSSQPSNGE NSPQIKPLI 
      KLVDINKLV EPRPQIKTD PREKLEKTV RFDDTVVLI 
      TSSDEDKQK DSPLIESVG KRKAAARTI KKTRQSLQV 
      GTSSSSESS DDTDFIVLK PTKSKTASV NRIRKKKTE 
      FMNKTVPDT DTDSSDDDD LETIKSRNS KNKKKSKKV 
      EEQKSSDEE SDEPFVDWK NDPKFNLKC YVKLIRIPC 
      EKLKEHYLG NLELLEIKR LTDLNSLNK KRRRSSSDN 
      SACSENQNS KKRKRKNTK SKENPEKDS SESTASDLD 
      EEINHSPVS EVEKNQDLA VGNSEEMAE DALNLLNSD 
      KDDSDDKEE APEENSENS DKEKNGKET AEKNLENEE 
      SVERPENVP DKDPLEIAN NSENVDKPD EASEKDPTN 
      ETNENEQDN TEKNDNGSE KTNDDQSEK EANDKSDEG 
      SKSDENKIE EKSKKRWRK DKLLTSKLS SSDSEEEFA 
      KFCRKKEKM NEEVARIMK KRLKKAILS DSDSDKKDS 
      GDDFQQKSS SSSSDSDEE DDKKKEEKP KPARKRVKR 
      VRDSSDDDD EEKSTRKQI RNIWGRDSL AESTKQAEA 
      EERERKARI AEKQKKYNQ IYEFSSQLN AKVDKVVLD 
      FDEKNQKEL LKVDDSLVS KLKPHQASG VQFMWDACF 
      ESLERAKST KGSGCILAH CMGLGKTLQ VITLSHTLL 
      INSEKTNVK KVLVVSPLN TVLNWVSEF KQWLPDCEE 
      YDVYELVSF KQNYERQYQ VKTWHDHGG VLIIGYDMF 
      RNLSNPDNK RLSKKMRAA FQEALVDPG PDLVICDEG 
      HLLKNEKTN TSIAMNRLK TLRRIVLTG TPLQNNLKE 
      YFCMVQFVK PNLLGTYKE YLNRFVNPI TNGQYTDST 
      PHDIQIMRK RSHVLHKML DGVVQRRDY SVLEPYLPP 
      KHEYVLFLS LTETQIKLY QHYMDRFAR SGDGSNRTS 
      FLFVDFQAL QRICTHPRV LLDKSIEMK LAKEKRDDE 
      SEESEGSLK DFINDDDEE EESTGSSNS SSDEGGEKK 
      SAAPRKRVT RAAAALAKE NGEPEEIIT LEDTVEKEW 
      WQEYCDGDE LNNIAHSSK LFLLFEILK ECEQIGDKV 
      LVFSQSLYS LNIIEYFLG RIHDATQAG ETDSVGGYS 
      GSWCVGLDY FRLDGSSSC DSRSMWCRT FNNPTNTRA 
      RLFLISTKA GGLGINLVA ANRVIIFDV SWNPSHDIQ 
      SIYRVYRFG QTKPCYIYR FVTLGTMEM KIYERQVTK 
      QAISKRVID EQQIDRHYN QNDLAELYK FDPKPGDRP 
      IPLVPKDVL LGELLQKFD RIYKYHEHQ TLLENKEEE 
      GLNEEERKA AWEEFENEK KQRHNYLGN TYLRAANVQ 
      LALANLLRK ENPAWTEMQ IKEMLPILQ AQMQEQVTR 
      GDLTLYKQV QHEVQMIQA IQNQRLKEQ FYQQQALRM 
      LQQQKMPQQ LAAAMNYHN AATWLEQYM NQMPGTSRQ 
      PPQAVNPND VIDLND
    CDS sequence
    • >TC009794 RA
      atgtcgaga gaatcttta aacgaagtg ttagagaag 
      atcgctcaa gtcacagca attttagtt gagacttct 
      aaaattaaa gaacagtta gacagatct gcaagcgac 
      gaaaaactg caagtgatt attgagcag actgaagtg 
      caagtcaac agtttagtg aaacacgca acttccgtt 
      aaggaattg tttgacaaa aatatgaaa acgtctgag 
      aaactcctg gtaaatgga gttgacata aacaaaaac 
      tctgaaacg aacggtaat gattcaagc cagccttcg 
      aacggtgaa aattcgccg caaataaag cctctgatt 
      aaattagtc gatattaac aaattagtg gagcctaga 
      cctcagatt aagacagat cctagggaa aaacttgaa 
      aaaactgtg agatttgat gacactgtt gtgctgata 
      actagctcc gatgaagac aaacagaaa gactcacct 
      ctcattgaa agtgtgggg aagaggaaa gctgcagct 
      agaactatc aaaaagaca agacagtcg ttgcaagtt 
      ggaacaagc tcaagttca gagtccagt gatgacacg 
      gacttcatt gtcttaaaa cccacgaaa agtaaaact 
      gcatccgtg aatagaatc agaaagaag aaaactgaa 
      tttatgaac aagaccgtg cctgatact gataccgac 
      agttctgat gatgacgat ttagaaaca attaaaagc 
      agaaatagt aaaaataaa aagaagtcg aagaaagtt 
      gaagaacag aagtcaagt gatgaagaa agtgacgag 
      ccttttgtg gactggaaa aacgacccc aaatttaac 
      ttaaagtgc tacgtcaag ctcattaga attccctgt 
      gaaaaactt aaagaacat tatttagga aacttggaa 
      ctgttggaa attaaaaga ttgacagac ttgaactcg 
      ttaaataaa aaaagaaga agatcaagt tcagataat 
      tcagcttgt agtgaaaat caaaatagt aaaaaaaga 
      aaaaggaaa aacactaaa agcaaagag aatcccgaa 
      aaagacagc tctgagtct actgcttct gaccttgat 
      gaagagata aatcatagt cctgtatct gaagttgag 
      aagaaccag gatttggct gttggaaat tcggaagaa 
      atggctgaa gacgctctt aatttgctt aattcggat 
      aaagacgat agtgatgat aaagaggag gctccagaa 
      gagaattct gaaaattcc gacaaggag aagaatggc 
      aaagagact gcagaaaag aatttagaa aatgaggaa 
      agtgttgaa cgtccagaa aatgtccca gataaggat 
      cctttagaa attgcaaat aatagtgaa aacgtagat 
      aaaccagat gaggcatcc gagaaagat cccacaaat 
      gaaacaaac gaaaatgag caagataat acggaaaaa 
      aacgataat ggtagcgaa aaaactaac gacgaccag 
      tcagaaaaa gaagccaac gataaatcc gacgagggc 
      tcaaaatcg gatgaaaac aaaattgaa gaaaaatcg 
      aaaaagcgt tggcgcaag gataaactc cttacttcc 
      aagctatca agcagtgac tccgaagaa gagtttgcg 
      aaattttgc agaaaaaaa gaaaaaatg aatgaggaa 
      gttgccagg ataatgaaa aagcgcctg aagaaagcg 
      attttgtca gactctgac tctgataaa aaagacagc 
      ggtgacgac tttcagcag aaatctagc agttcgtcc 
      tcagactcg gacgaagag gacgacaaa aagaaagag 
      gagaagcca aaaccggca cgaaaacga gtaaaacgc 
      gtcagagac agtagcgac gatgacgat gaagaaaaa 
      tcaacacga aaacaaatc cgaaacatt tgggggcga 
      gactccctc gctgaaagc acgaaacag gccgaagct 
      gaagaaagg gaacgaaag gcccgaatt gccgaaaaa 
      cagaagaag tacaaccaa atctacgaa ttcagttcg 
      caactgaac gcgaaagtt gataaagtc gttcttgac 
      tttgacgaa aagaaccag aaagaattg ctaaaagtc 
      gacgatagt ttagtctcg aaattgaag ccgcaccaa 
      gccagtggc gtccagttc atgtgggac gcatgcttc 
      gagtccttg gaacgggcg aaaagcacc aaaggctcg 
      ggttgcatc ctggcacat tgtatgggt ctcggaaaa 
      acgttacag gtcataacc ttgagtcac actttacta 
      atcaacagc gagaaaaca aacgtgaaa aaagtccta 
      gtcgtgagc cccctgaac acggtcctg aattgggtc 
      agcgagttc aagcagtgg ctacctgat tgtgaggag 
      tatgacgtc tacgaactg gtctcattc aagcaaaat 
      tacgaacgg cagtatcaa gtgaaaacc tggcacgat 
      cacggcggt gttttaatc attggttac gacatgttt 
      cgtaatttg agtaatccg gataataag cggttgagc 
      aaaaaaatg cgggccgcc tttcaggaa gccttggtt 
      gatccaggc ccggatttg gtgatttgt gacgaaggc 
      cacttgctc aaaaacgag aagactaac acaagtatt 
      gcaatgaat cggttgaaa acgttgcga aggattgtc 
      ttgaccggg acgccgctg cagaataat ttgaaagag 
      tatttctgc atggttcag ttcgtgaaa ccgaatttg 
      ttggggacg tataaagaa tatttgaat cggtttgtt 
      aaccccatc actaatggg caatataca gactcaacc 
      ccgcacgat attcaaatt atgcgtaaa agatcgcac 
      gttttgcac aaaatgctg gatggtgtc gtgcaaaga 
      cgagactac tcagttttg gagccgtac ttgccaccg 
      aaacacgaa tatgtacta tttttgagt ctcactgag 
      acgcagatc aagttgtac caacattac atggaccgt 
      tttgcgcga agtggggac ggttcgaac cgtacgtcg 
      tttttgttt gtcgatttt caggccttg caaaggatt 
      tgcacccat ccgagggtg ctccttgac aagagtatt 
      gaaatgaag ttggctaag gaaaaacgg gatgatgaa 
      tcggaagag tcggaaggt tcgttgaaa gattttatc 
      aatgatgac gacgaagag gaagaatct acgggatcg 
      tcaaattcg agttcggac gaaggtggt gagaaaaag 
      agtgcagca ccgaggaaa cgcgtaaca agagctgcc 
      gccgcgtta gcaaaagag aatggggag ccggaagaa 
      attatcact cttgaagac accgtggaa aaggagtgg 
      tggcaggaa tattgcgac ggtgatgaa ctgaacaat 
      attgcgcac agttcgaag ctgtttctg ttatttgag 
      attctcaaa gagtgtgaa caaataggc gacaaagta 
      ctggtattt tcgcagtcg ctttattcc ctcaacata 
      atcgagtat ttccttgga cggattcac gacgccacg 
      caggctggc gaaacggac tccgtaggg ggctatagt 
      gggtcgtgg tgtgtggga ttggattat ttcaggtta 
      gatggctcg tcatcgtgt gacagtcgt agcatgtgg 
      tgtagaact ttcaacaac cccacaaac acccgagct 
      agattattt ttaatttct accaaagcc ggcggttta 
      ggtattaat ttggtcgcc gccaatcgt gttattatt 
      ttcgatgtc tcatggaac ccctcgcac gatattcag 
      agcatatac agggtgtat cggtttgga caaacgaaa 
      ccgtgctac atttatcgt ttcgtgact ttgggcaca 
      atggaaatg aaaatttac gagagacag gttacgaaa 
      caagcgatt tccaaacgt gttatcgat gaacaacaa 
      attgaccga cactacaat cagaatgat ctcgcagaa 
      ttatacaaa tttgaccca aaacctggt gacagacca 
      atcccgctt gtaccaaaa gatgtactc ctaggagaa 
      ctcctacag aagtttgat cggatttac aaataccac 
      gaacatcaa acgctactt gaaaataag gaagaggag 
      ggtcttaat gaggaagag cgtaaagca gcgtgggag 
      gagttcgaa aatgagaag aaacaaaga cacaattat 
      ttgggtaac acttatttg cgcgccgct aatgtccaa 
      ctggctttg gccaatttg ttaagaaaa gagaatccg 
      gcttggacc gaaatgcaa ataaaagaa atgttgcct 
      attttacaa gcccagatg caggaacaa gtcacgcgg 
      ggcgacctg actttgtac aaacaagtc cagcatgaa 
      gtacaaatg atccaagcc atccaaaac cagagactc 
      aaagaacag ttctaccaa cagcaagcc ttgaggatg 
      ctacaacaa caaaaaatg cctcagcag ctagcagca 
      gctatgaac tatcacaac gcggctact tggttggag 
      cagtacatg aatcagatg cctggcaca agtcgccaa 
      cccccacag gcggttaac ccgaacgac gttatagat 
      ttaaatgat tag
    mRNA sequence
    • >TC009794 RA
      tttgtgcgg aatgctaat ggccgtcag tttattgtt 
      tgatttgtt ggactagat gaaaaaatg gtaaaatgc 
      cgcattgat ttaaaacta aatcgcttt aaagatgat 
      tcggcggct tagcaagtc cttgtaaaa gtgttgtgt 
      atgtggctg tgtcaatat tattgtcag actgtacag 
      gcgggaaac actcaatac tattcggta aaatttagg 
      gtttagtcc tgcaaaaga tgtcgagag aatctttaa 
      acgaagtgt tagagaaga tcgctcaag tcacagcaa 
      ttttagttg agacttcta aaattaaag aacagttag 
      acagatctg caagcgacg aaaaactgc aagtgatta 
      ttgagcaga ctgaagtgc aagtcaaca gtttagtga 
      aacacgcaa cttccgtta aggaattgt ttgacaaaa 
      atatgaaaa cgtctgaga aactcctgg taaatggag 
      ttgacataa acaaaaact ctgaaacga acggtaatg 
      attcaagcc agccttcga acggtgaaa attcgccgc 
      aaataaagc ctctgatta aattagtcg atattaaca 
      aattagtgg agcctagac ctcagatta agacagatc 
      ctagggaaa aacttgaaa aaactgtga gatttgatg 
      acactgttg tgctgataa ctagctccg atgaagaca 
      aacagaaag actcacctc tcattgaaa gtgtgggga 
      agaggaaag ctgcagcta gaactatca aaaagacaa 
      gacagtcgt tgcaagttg gaacaagct caagttcag 
      agtccagtg atgacacgg acttcattg tcttaaaac 
      ccacgaaaa gtaaaactg catccgtga atagaatca 
      gaaagaaga aaactgaat ttatgaaca agaccgtgc 
      ctgatactg ataccgaca gttctgatg atgacgatt 
      tagaaacaa ttaaaagca gaaatagta aaaataaaa 
      agaagtcga agaaagttg aagaacaga agtcaagtg 
      atgaagaaa gtgacgagc cttttgtgg actggaaaa 
      acgacccca aatttaact taaagtgct acgtcaagc 
      tcattagaa ttccctgtg aaaaactta aagaacatt 
      atttaggaa acttggaac tgttggaaa ttaaaagat 
      tgacagact tgaactcgt taaataaaa aaagaagaa 
      gatcaagtt cagataatt cagcttgta gtgaaaatc 
      aaaatagta aaaaaagaa aaaggaaaa acactaaaa 
      gcaaagaga atcccgaaa aagacagct ctgagtcta 
      ctgcttctg accttgatg aagagataa atcatagtc 
      ctgtatctg aagttgaga agaaccagg atttggctg 
      ttggaaatt cggaagaaa tggctgaag acgctctta 
      atttgctta attcggata aagacgata gtgatgata 
      aagaggagg ctccagaag agaattctg aaaattccg 
      acaaggaga agaatggca aagagactg cagaaaaga 
      atttagaaa atgaggaaa gtgttgaac gtccagaaa 
      atgtcccag ataaggatc ctttagaaa ttgcaaata 
      atagtgaaa acgtagata aaccagatg aggcatccg 
      agaaagatc ccacaaatg aaacaaacg aaaatgagc 
      aagataata cggaaaaaa acgataatg gtagcgaaa 
      aaactaacg acgaccagt cagaaaaag aagccaacg 
      ataaatccg acgagggct caaaatcgg atgaaaaca 
      aaattgaag aaaaatcga aaaagcgtt ggcgcaagg 
      ataaactcc ttacttcca agctatcaa gcagtgact 
      ccgaagaag agtttgcga aattttgca gaaaaaaag 
      aaaaaatga atgaggaag ttgccagga taatgaaaa 
      agcgcctga agaaagcga ttttgtcag actctgact 
      ctgataaaa aagacagcg gtgacgact ttcagcaga 
      aatctagca gttcgtcct cagactcgg acgaagagg 
      acgacaaaa agaaagagg agaagccaa aaccggcac 
      gaaaacgag taaaacgcg tcagagaca gtagcgacg 
      atgacgatg aagaaaaat caacacgaa aacaaatcc 
      gaaacattt gggggcgag actccctcg ctgaaagca 
      cgaaacagg ccgaagctg aagaaaggg aacgaaagg 
      cccgaattg ccgaaaaac agaagaagt acaaccaaa 
      tctacgaat tcagttcgc aactgaacg cgaaagttg 
      ataaagtcg ttcttgact ttgacgaaa agaaccaga 
      aagaattgc taaaagtcg acgatagtt tagtctcga 
      aattgaagc cgcaccaag ccagtggcg tccagttca 
      tgtgggacg catgcttcg agtccttgg aacgggcga 
      aaagcacca aaggctcgg gttgcatcc tggcacatt 
      gtatgggtc tcggaaaaa cgttacagg tcataacct 
      tgagtcaca ctttactaa tcaacagcg agaaaacaa 
      acgtgaaaa aagtcctag tcgtgagcc ccctgaaca 
      cggtcctga attgggtca gcgagttca agcagtggc 
      tacctgatt gtgaggagt atgacgtct acgaactgg 
      tctcattca agcaaaatt acgaacggc agtatcaag 
      tgaaaacct ggcacgatc acggcggtg ttttaatca 
      ttggttacg acatgtttc gtaatttga gtaatccgg 
      ataataagc ggttgagca aaaaaatgc gggccgcct 
      ttcaggaag ccttggttg atccaggcc cggatttgg 
      tgatttgtg acgaaggcc acttgctca aaaacgaga 
      agactaaca caagtattg caatgaatc ggttgaaaa 
      cgttgcgaa ggattgtct tgaccggga cgccgctgc 
      agaataatt tgaaagagt atttctgca tggttcagt 
      tcgtgaaac cgaatttgt tggggacgt ataaagaat 
      atttgaatc ggtttgtta accccatca ctaatgggc 
      aatatacag actcaaccc cgcacgata ttcaaatta 
      tgcgtaaaa gatcgcacg ttttgcaca aaatgctgg 
      atggtgtcg tgcaaagac gagactact cagttttgg 
      agccgtact tgccaccga aacacgaat atgtactat 
      ttttgagtc tcactgaga cgcagatca agttgtacc 
      aacattaca tggaccgtt ttgcgcgaa gtggggacg 
      gttcgaacc gtacgtcgt ttttgtttg tcgattttc 
      aggccttgc aaaggattt gcacccatc cgagggtgc 
      tccttgaca agagtattg aaatgaagt tggctaagg 
      aaaaacggg atgatgaat cggaagagt cggaaggtt 
      cgttgaaag attttatca atgatgacg acgaagagg 
      aagaatcta cgggatcgt caaattcga gttcggacg 
      aaggtggtg agaaaaaga gtgcagcac cgaggaaac 
      gcgtaacaa gagctgccg ccgcgttag caaaagaga 
      atggggagc cggaagaaa ttatcactc ttgaagaca 
      ccgtggaaa aggagtggt ggcaggaat attgcgacg 
      gtgatgaac tgaacaata ttgcgcaca gttcgaagc 
      tgtttctgt tatttgaga ttctcaaag agtgtgaac 
      aaataggcg acaaagtac tggtatttt cgcagtcgc 
      tttattccc tcaacataa tcgagtatt tccttggac 
      ggattcacg acgccacgc aggctggcg aaacggact 
      ccgtagggg gctatagtg ggtcgtggt gtgtgggat 
      tggattatt tcaggttag atggctcgt catcgtgtg 
      acagtcgta gcatgtggt gtagaactt tcaacaacc 
      ccacaaaca cccgagcta gattatttt taatttcta 
      ccaaagccg gcggtttag gtattaatt tggtcgccg 
      ccaatcgtg ttattattt tcgatgtct catggaacc 
      cctcgcacg atattcaga gcatataca gggtgtatc 
      ggtttggac aaacgaaac cgtgctaca tttatcgtt 
      tcgtgactt tgggcacaa tggaaatga aaatttacg 
      agagacagg ttacgaaac aagcgattt ccaaacgtg 
      ttatcgatg aacaacaaa ttgaccgac actacaatc 
      agaatgatc tcgcagaat tatacaaat ttgacccaa 
      aacctggtg acagaccaa tcccgcttg taccaaaag 
      atgtactcc taggagaac tcctacaga agtttgatc 
      ggatttaca aataccacg aacatcaaa cgctacttg 
      aaaataagg aagaggagg gtcttaatg aggaagagc 
      gtaaagcag cgtgggagg agttcgaaa atgagaaga 
      aacaaagac acaattatt tgggtaaca cttatttgc 
      gcgccgcta atgtccaac tggctttgg ccaatttgt 
      taagaaaag agaatccgg cttggaccg aaatgcaaa 
      taaaagaaa tgttgccta ttttacaag cccagatgc 
      aggaacaag tcacgcggg gcgacctga ctttgtaca 
      aacaagtcc agcatgaag tacaaatga tccaagcca 
      tccaaaacc agagactca aagaacagt tctaccaac 
      agcaagcct tgaggatgc tacaacaac aaaaaatgc 
      ctcagcagc tagcagcag ctatgaact atcacaacg 
      cggctactt ggttggagc agtacatga atcagatgc 
      ctggcacaa gtcgccaac ccccacagg cggttaacc 
      cgaacgacg ttatagatt taaatgatt agttttaag 
      atttcgtca tatgttgtg gtagatatc tcatagtcc 
      aaaattttg tacatagtt ttgttttgt tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY