Status
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Gene information
General information
TC014513 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6421865..6430402
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014513
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10328

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014513 RA
      MTHLKVGVR VRPLSRREF EGNSSTILE VEDDGKTVA 
      VTNIKVPEQ NAGDSRKRI RRFTFDFCL HENSTQDDV 
      FQAIENVIS KAIKRRYHS CVLAYGQSS SGKTHTMMG 
      FPHDPGVTP KLCQKVFDY LQESAVGSE MEKTKVTCS 
      YLEIYNEKV RDLLLKDDT CALRIRQHP KKGPYVQGL 
      SSHPAPDSS TLLRLLSTG NSNRRVGAT LTNPQSSRS 
      HSVFIIQFD EIKLTLVDL AGSERTGNR CYTASRFRE 
      GANINKSLV ALGNVISAL AEDTAKSSK GIRRRFIPF 
      RDSVLTWLL KDTLGGNSD TVMIATISP SSECYSETV 
      NTLRFGQRA KLIVSIPVV NEDPKERTI RELRAEIAR 
      LRELLSLGQ FQKPMSNDN LNTTEKLIP VMSFTDSNK 
      PEPSRLRRT SSIDHPLKK PIVPTRKYS SEEALRRAK 
      PPKNEKSSL EPKPKVEAK VDSIRRAPV KPRSQIVAA 
      VTHRLYAKT KKKEVATDT SDLPTGPPK ELSICSNAR 
      LQLKELTRR ALKAHKFRN EETQTELFP VLRVKETST 
      DVDDLKSLL YEVRDVETS TETLPMEDK EVSCAFLDS 
      LQNAFVVTK SCGTQAQVQ SSVSFTKYL QEPPRIEIF 
      NPHTANPIY TSSVNINIS HNYINGRKI TESVSDDSL 
      DDQSNVCFP TPDLISNHN SLEPSKETL ESKLRHATS 
      NTLEQEEGL AFDLVQDNP QCFVANTST VPECQERVS 
      CVNIPSIYA PEVCHQEET LHRCEVFTP EVPEMAKPN 
      GSIQPEDLP DSLEYHMAN SKKVHFNKS GTCQNDRMM 
      KAMSDFLEE ATILMNNLS KAAIQDQER EYDVQVSIN 
      GLVEQQKRR RKRRVLKNV KCQTEEAAT KSNCIQTED 
      DLDLTNKYE LLLEDSCRR LEEKIAAAT EESIYNPWD 
      LYHHEDTSL ESNPVTFSD YGSLPRRKK RYTSCTPSA 
      YLRQLASMR RQVVEASRE ELSNHSDSV S
    CDS sequence
    • >TC014513 RA
      atgactcac cttaaggtt ggagtacga gtacgacca 
      ctctctcga agagagttc gagggcaat tcctccacg 
      attttggaa gttgaggac gatgggaaa actgtggcc 
      gtgacgaat atcaaggta cccgaacag aatgccggc 
      gacagtcgc aagcgcatc agaagattc accttcgac 
      ttctgcttg cacgagaat tccacacag gatgatgtg 
      ttccaagct attgaaaat gtcatcagc aaagcgatc 
      aaaaggcgg tatcattca tgcgttttg gcctatgga 
      caaagctcc tccggaaag acgcacacc atgatgggg 
      tttccgcac gatccaggg gtcacaccc aaattgtgc 
      caaaaggta ttcgactat ttgcaagaa agtgcagtg 
      ggaagcgaa atggagaag acaaaagta acctgcagt 
      tatttggag atatacaat gagaaagtg agggatttg 
      ctgcttaag gatgacacg tgcgcgctc aggataagg 
      caacatccg aagaaagga ccttacgtt caaggcctg 
      tcctcgcac ccagcgccg gactccagc actcttctt 
      cgcctcctc agcacaggc aacagcaac cgtcgtgtg 
      ggtgccacc ctcaccaac cctcagtcc agccgcagc 
      cactccgtc ttcatcatc caattcgac gaaatcaaa 
      ctcaccctc gtcgatcta gccggaagt gaacgcacc 
      ggaaacagg tgctacacc gcatcccgc ttccgagag 
      ggtgctaac atcaacaaa agtttagtg gccttgggg 
      aacgtcatc tcggccttg gccgaagac acggccaag 
      agctccaag gggatcagg aggaggttt attccgttc 
      agggattcg gtgctgaca tggctatta aaagacacg 
      ctgggagga aactccgac actgtgatg attgcgact 
      atttctcca tccagtgag tgctatagt gagacggtg 
      aatactttg aggtttggg caacgggcc aagctaatc 
      gtgtccatt ccagtcgtt aatgaagac cccaaggag 
      cggaccatc agggagcta agagctgaa atcgcaagg 
      ctcagggag ctgctcagc ctgggacag ttccagaaa 
      cccatgtct aacgataac cttaacacc accgagaaa 
      ctcatccct gtgatgagt ttcactgat tccaacaaa 
      ccagaaccc tcccgtctt cgacgcacc tccagcatt 
      gaccacccc ctcaaaaaa cccattgta ccaactcgc 
      aaatacagc tcggaggaa gctttacgc agagctaaa 
      ccacccaaa aatgaaaag agtagttta gaacccaaa 
      cctaaagtc gaggccaaa gtcgactca atccgacga 
      gctccagtc aaaccacga tcacaaatc gtggcagcg 
      gtcacacac cgactttac gccaaaacc aagaaaaaa 
      gaagtcgcg actgacact tccgaccta ccaacaggc 
      cccccaaaa gagttgagc atttgttca aatgctcgg 
      ctccagctg aaggaactg acaaggcgg gcgcttaaa 
      gcacacaaa ttccgaaat gaggaaacc caaacggaa 
      cttttccct gttttgaga gtcaaagaa acatcgact 
      gatgtcgac gacttgaaa tctctgttg tacgaagta 
      cgagatgtt gaaacttcg actgaaact ctgccaatg 
      gaagacaaa gaagtcagt tgtgcgttt cttgacagt 
      ttgcaaaat gcgtttgtt gtgacgaaa agttgtggt 
      actcaagcc caagtccag tcgagtgtt tccttcact 
      aaatatctc caagaacca ccgagaatt gaaatattc 
      aacccccac actgccaat ccgatttac acttcttca 
      gtcaatatc aacatttcg cacaattat atcaacggg 
      cgcaaaatt accgaaagt gtttctgat gatagtttg 
      gatgaccaa tcaaatgtt tgtttcccc acgcctgat 
      ttaataagt aaccacaac tcgttagaa ccgtcgaaa 
      gaaactctc gagagtaaa ttgcgtcat gcgacttct 
      aacactctc gaacaagag gaaggtcta gcatttgat 
      ttggtgcag gacaacccc cagtgtttt gttgccaac 
      acttccacg gttccagag tgtcaagaa cgtgtcagt 
      tgtgttaac attccgagt atttatgct ccagaagtt 
      tgccaccaa gaggagact ctccataga tgcgaggtc 
      ttcactcca gaagtgcca gagatggct aaacccaat 
      ggaagcata caaccggag gatcttcca gactcactg 
      gagtatcac atggcaaac tccaagaaa gtccatttt 
      aataaatct ggaacctgt caaaatgac cgcatgatg 
      aaagccatg tccgatttt ctggaagaa gcaactatt 
      ttgatgaac aatttgtcg aaggctgcg attcaggac 
      caggaacgt gaatacgac gtccaggtc tccattaat 
      ggtttggtg gaacaacaa aaacgaagg aggaagaga 
      cgagtcctc aagaacgtt aaatgtcaa actgaagaa 
      gcagcgacg aaatcgaat tgcattcaa actgaggat 
      gacttggac cttaccaac aagtatgag ttactgttg 
      gaggattct tgccgaaga ctagaagaa aaaattgct 
      gccgccacc gaagaaagc atttacaat ccgtgggat 
      ttgtatcac catgaagac acaagtctg gagagcaat 
      cccgtcact ttctcggac tatggaagc ttgcccaga 
      cgcaaaaag agatacact tcgtgtacc cccagcgcg 
      tatttgaga cagctggcg agtatgaga cggcaagtg 
      gtcgaagcc tcgcgtgag gaactttca aatcacagc 
      gacagtgtt tcgtaa
    mRNA sequence
    • >TC014513 RA
      gagtcgatc cagatgtcg gctgtgtct ccgtcagtg 
      tgtataagt gttctgaaa gaagatgac tcaccttaa 
      ggttggagt acgagtacg accactctc tcgaagaga 
      gttcgaggg caattcctc cacgatttt ggaagttga 
      ggacgatgg gaaaactgt ggccgtgac gaatatcaa 
      ggtacccga acagaatgc cggcgacag tcgcaagcg 
      catcagaag attcacctt cgacttctg cttgcacga 
      gaattccac acaggatga tgtgttcca agctattga 
      aaatgtcat cagcaaagc gatcaaaag gcggtatca 
      ttcatgcgt tttggccta tggacaaag ctcctccgg 
      aaagacgca caccatgat ggggtttcc gcacgatcc 
      aggggtcac acccaaatt gtgccaaaa ggtattcga 
      ctatttgca agaaagtgc agtgggaag cgaaatgga 
      gaagacaaa agtaacctg cagttattt ggagatata 
      caatgagaa agtgaggga tttgctgct taaggatga 
      cacgtgcgc gctcaggat aaggcaaca tccgaagaa 
      aggacctta cgttcaagg cctgtcctc gcacccagc 
      gccggactc cagcactct tcttcgcct cctcagcac 
      aggcaacag caaccgtcg tgtgggtgc caccctcac 
      caaccctca gtccagccg cagccactc cgtcttcat 
      catccaatt cgacgaaat caaactcac cctcgtcga 
      tctagccgg aagtgaacg caccggaaa caggtgcta 
      caccgcatc ccgcttccg agagggtgc taacatcaa 
      caaaagttt agtggcctt ggggaacgt catctcggc 
      cttggccga agacacggc caagagctc caaggggat 
      caggaggag gtttattcc gttcaggga ttcggtgct 
      gacatggct attaaaaga cacgctggg aggaaactc 
      cgacactgt gatgattgc gactatttc tccatccag 
      tgagtgcta tagtgagac ggtgaatac tttgaggtt 
      tgggcaacg ggccaagct aatcgtgtc cattccagt 
      cgttaatga agaccccaa ggagcggac catcaggga 
      gctaagagc tgaaatcgc aaggctcag ggagctgct 
      cagcctggg acagttcca gaaacccat gtctaacga 
      taaccttaa caccaccga gaaactcat ccctgtgat 
      gagtttcac tgattccaa caaaccaga accctcccg 
      tcttcgacg cacctccag cattgacca ccccctcaa 
      aaaacccat tgtaccaac tcgcaaata cagctcgga 
      ggaagcttt acgcagagc taaaccacc caaaaatga 
      aaagagtag tttagaacc caaacctaa agtcgaggc 
      caaagtcga ctcaatccg acgagctcc agtcaaacc 
      acgatcaca aatcgtggc agcggtcac acaccgact 
      ttacgccaa aaccaagaa aaaagaagt cgcgactga 
      cacttccga cctaccaac aggcccccc aaaagagtt 
      gagcatttg ttcaaatgc tcggctcca gctgaagga 
      actgacaag gcgggcgct taaagcaca caaattccg 
      aaatgagga aacccaaac ggaactttt ccctgtttt 
      gagagtcaa agaaacatc gactgatgt cgacgactt 
      gaaatctct gttgtacga agtacgaga tgttgaaac 
      ttcgactga aactctgcc aatggaaga caaagaagt 
      cagttgtgc gtttcttga cagtttgca aaatgcgtt 
      tgttgtgac gaaaagttg tggtactca agcccaagt 
      ccagtcgag tgtttcctt cactaaata tctccaaga 
      accaccgag aattgaaat attcaaccc ccacactgc 
      caatccgat ttacacttc ttcagtcaa tatcaacat 
      ttcgcacaa ttatatcaa cgggcgcaa aattaccga 
      aagtgtttc tgatgatag tttggatga ccaatcaaa 
      tgtttgttt ccccacgcc tgatttaat aagtaacca 
      caactcgtt agaaccgtc gaaagaaac tctcgagag 
      taaattgcg tcatgcgac ttctaacac tctcgaaca 
      agaggaagg tctagcatt tgatttggt gcaggacaa 
      cccccagtg ttttgttgc caacacttc cacggttcc 
      agagtgtca agaacgtgt cagttgtgt taacattcc 
      gagtattta tgctccaga agtttgcca ccaagagga 
      gactctcca tagatgcga ggtcttcac tccagaagt 
      gccagagat ggctaaacc caatggaag catacaacc 
      ggaggatct tccagactc actggagta tcacatggc 
      aaactccaa gaaagtcca ttttaataa atctggaac 
      ctgtcaaaa tgaccgcat gatgaaagc catgtccga 
      ttttctgga agaagcaac tattttgat gaacaattt 
      gtcgaaggc tgcgattca ggaccagga acgtgaata 
      cgacgtcca ggtctccat taatggttt ggtggaaca 
      acaaaaacg aaggaggaa gagacgagt cctcaagaa 
      cgttaaatg tcaaactga agaagcagc gacgaaatc 
      gaattgcat tcaaactga ggatgactt ggaccttac 
      caacaagta tgagttact gttggagga ttcttgccg 
      aagactaga agaaaaaat tgctgccgc caccgaaga 
      aagcattta caatccgtg ggatttgta tcaccatga 
      agacacaag tctggagag caatcccgt cactttctc 
      ggactatgg aagcttgcc cagacgcaa aaagagata 
      cacttcgtg tacccccag cgcgtattt gagacagct 
      ggcgagtat gagacggca agtggtcga agcctcgcg 
      tgaggaact ttcaaatca cagcgacag tgtttcgta 
      acaataata ttggcaagt aattgtatg aacttttat 
      taccatatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY