| General information | |||
| TC014516 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 6404679..6414672 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014516 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10327 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014516 RA
MSDLLSDFL NDRLQPVPV EGVVNGIMD YEKPEENTY NSSDSESES DTEQQEYIK KELSTYFPE TEAPCSGGL TDIEDGHEE SVLGTDIME LLHVPKVFE TYKFDTYYK KELPIDSSR DKILDMINT NSVVIIHGP TGCGKTTQV PQYILDHCR ATKSPCNIV VTQPRRIAA INIAQRVCE ERGWAIGTV CGYQVGLDK NVGDDVILT YMTTEVLLQ KLISQKNLN RFTHVIIDE VHERSKSLD FLLLIVRKY LFTNSSSVK IILMSATME AQDFAYYFR SISRNNPQQ YLLAPVLPV TKKSEYKVS IYYNEHFAS AMPPYNFEE PCMHKEQYD VCAKLISLF DKLEENESK LTLAERING SVLVFLPGF HEIEEMHKV LVRERNTSV LEWEIIPLH SSLAQEHMV KAFQKPRQR CRKIILSTN IAESSVTVP DVNFVIDFC LTKNMTVNE VTKFSSLSL QWASYTNCI QRAGRVGRV ANGRVYRVV PTSFYLHEM KQTTVPELQ RAPLENVIL YMKLLGLND TPKNVLSLA LSPPNLKDV EQCVWHLKE VGALLQTCR GHRTPADGD ITFMGRVMG SLPIDIHLS KLILLGHMF SCLDEAVIM AAGCMTKNI FVQNFYDRF RTYRQKLVW ADGSHSDFM ILLNLYNVW LSMKRDRAF SSSHQEIGW CKTHFVNLK GLREWDILI QEIHSRLKR LNIQKLPGP SSIPLSIVE KPMVLKVII CGAFYPYYF IKSSDFGNV DAKEAVKIL NGRDPCNTV YFTNMKMNQ PGQIYVRQI KKLMNCEDK PNVQIGFDP QSTKVFVEF KATRQPEQV TIDGRQYIA TVASNIAVD VYEAVRKRQ MRVPFVLRI LPDSKAWEF ANMTQAKRQ IAESEDVNC FTTLDYSPL PTLDIEYIT VTVTHIIDA GHFYCQNWN EETRMLLDQ IFAALNGPG VFLEPAGEK IKVNSDIYA ALFNEDGKF YRCKVIDLT PGQPNVAQV CFIDYGNVQ RVPKNRLYK LPENSEPCR VQPIAMCCV LSGVQPDLV LNPKALWSE SVNNILRKK TTGVLLNAK VFSVVDEVV HLELFLQNP GRNSVSFNQ WLINEGLGQ KCEESQRSK MDHEMRLKV QSSEDPSNM SALFNKNQI VTSYADFEA PESSEATEI IELKGPFSP LEMKVCGLV QASQGAPVH VDGDSVNAV MLDDNPEDY HATLLVAGQ VSQSSTTSA VKISQTTIM PNIPGFPML MCLLFCPQM EPKLTPDGS RVASILCGL GYKEVTQRA NFPMHDICL VLDTDLRSE IITKINALR YYMNEAIKI MSQIQEELA RPEEMYTTQ RFLKDELFH LLHMRQQTV DRVNVRYPD VWNKGLDNM EILRIDMDD DEEAIWSYL WFVKFGEDR LSKMSINKN LDELTQIAR RTAPEREIK CELCNSATL RNISDVRIH LFREEHKSN LAKYLNQNK
- >TC014516 RA
atgagtgat ttactttcc gatttttta aacgacagg cttcaacct gtccctgtg gaaggtgtt gtgaatgga atcatggat tatgaaaaa ccagaagaa aatacctac aactcttcc gattccgaa tcagaatca gacactgaa caacaagag tacattaag aaagaattg agtacctac tttcctgag acagaggca ccttgttct ggtggtctc accgatatc gaggatggc cacgaggag tctgttctg ggaacagac atcatggaa cttcttcac gttcctaaa gtttttgaa acctacaaa tttgacact tattacaaa aaggaatta ccaattgac tcgtctcgt gataaaatt cttgacatg attaacact aactccgtt gtaataatt cacgggcct acaggctgt ggtaaaact actcaagtg ccacagtac attttggac cactgtaga gccacaaaa tcgccttgc aatattgta gtaactcaa cctagaaga atagctgct ataaatatt gcacaacga gtgtgcgaa gagcgaggg tgggccatt ggaacagtt tgtggttat caagtaggt cttgataaa aacgtcggt gatgatgtg attttaact tatatgaca actgaggtt ctgttacaa aaattgatt tcccagaaa aatttgaac agatttact catgtgatt atcgatgaa gtacacgaa cggagcaaa tctctcgat tttttgctt ttgattgtg aggaaatat ctttttact aactcatct tcagttaag ataattctt atgtctgca actatggaa gcacaagat tttgcttac tattttcga agtatttca cgcaacaac ccccagcag tacttacta gctccggta ttacccgtc actaaaaaa agtgaatac aaagtcagc atttattac aatgaacat tttgcttct gcgatgcct ccgtacaat tttgaagag ccttgcatg cacaaagag cagtacgat gtttgtgcc aaattgatt agtcttttt gacaagttg gaagaaaat gagagcaag ttgacgctt gccgaacgg attaatgga agtgttttg gtgtttttg ccaggtttt catgaaatt gaagaaatg cataaagtt ttagtcagg gagagaaac acttccgtt ctcgaatgg gaaattatt cccttgcac tcgtctctg gcacaggaa cacatggtc aaagccttc cagaaacca agacaacga tgcagaaaa ataatcctg tcgactaat attgccgaa agttcggtt acagttcct gatgtcaat ttcgtgatt gacttctgt ctcaccaag aatatgact gtgaatgaa gtcacaaag ttttcaagt ttaagtctg cagtgggct tcatataca aactgcatt cagagggcg gggagagtt ggccgtgtt gccaatggc agagtgtat cgtgtggtc cccactagc ttttacctc cacgaaatg aaacaaact actgttcct gagctgcaa agagcgcct ttagagaat gtaattctg tatatgaag ttactggga ttgaacgac actcccaaa aatgttctt tctttggcc ctaagtcct ccaaatttg aaggatgtg gaacagtgt gtgtggcac ttgaaagaa gtgggagct ttgctgcaa acttgtaga ggccaccgc actcccgct gatggtgac ataactttc atgggacgc gtgatgggt tcgcttccc attgacata catttgtcc aaacttatt ctcttaggc cacatgttt agttgcctg gatgaagct gtgatcatg gctgctggt tgtatgacg aaaaatata ttcgttcaa aacttttac gacaggttc aggacttac agacagaaa cttgtatgg gctgatggg tctcatagc gactttatg attttatta aatttgtac aatgtttgg ctgagtatg aaacgagat agagccttc agttcgagc caccaagaa atcggttgg tgtaagacg catttcgtc aatttgaaa ggacttaga gaatgggat atcctaatc caagagatt cactcgcgc ttaaaacga ctcaatatc caaaaattg ccaggaccg tccagtatt cctctgtcc attgttgaa aaacccatg gttctgaaa gttataatc tgtggcgca ttctatcct tactatttc ataaaatca tcagatttt ggaaatgtc gacgcgaaa gaagctgtg aaaattctc aacggtcgc gatccctgc aacacggtt tatttcact aatatgaaa atgaaccaa ccaggacag atttatgtc agacaaata aaaaaacta atgaattgc gaagataaa ccaaacgtc cagattgga ttcgatcct cagagcacc aaagttttt gtcgaattt aaagccacc agacaaccg gaacaagtc acaattgat ggtagacag tacatcgct acggttgcg agtaacata gcggtggat gtgtacgaa gctgtgagg aagaggcaa atgagagtt cctttcgtg ttgcgtatt ttaccggat agcaaggca tgggagttt gctaatatg actcaggcg aaacgacag attgcggaa agtgaagat gttaattgt ttcacgact ctcgactat tcacctttg cctacccta gatatagaa tatatcact gtcactgta actcatatt attgacgcg ggccacttt tattgtcag aactggaat gaagaaact cgcatgctt ctagaccaa attttcgcg gctttgaac ggtccagga gtattccta gaacctgcc ggagagaaa atcaaagtc aacagcgac atatacgca gcgttgttt aatgaggac gggaaattt tacagatgc aaagtcatt gacttaaca cctggtcaa ccaaacgtt gcacaggtg tgttttatc gattatggg aacgtgcaa agagttccc aaaaaccga ctttataaa ttgcctgaa aacagtgag ccatgtcgg gttcaacca atcgcgatg tgttgcgtc cttagcgga gtgcaacca gaccttgtc ctaaatccc aaagctttg tggagtgaa agtgtcaac aatatccta cggaagaaa acgactggt gttttgtta aatgcaaaa gtattctcg gtggtagat gaggtcgtg catctcgaa ttattcctg caaaatcct ggtcggaat tcagtctcg ttcaatcag tggttgatt aacgaagga ctcggccaa aaatgcgaa gaaagtcaa cgatcaaaa atggaccac gaaatgcgt ctcaaagtc caaagctct gaagatcca agcaacatg agcgcgctc ttcaacaaa aatcaaatt gtgaccagt tacgccgat ttcgaagcg cctgaatcg agcgaagcc actgaaata atcgaactg aagggtcct ttcagccct cttgagatg aaagtttgc ggtctggtt caggccagt cagggggca cctgtgcac gttgatggt gattcagtc aacgctgtg atgctagac gacaatcct gaggattat cacgccacg ttgctcgtt gccggacaa gtctcccag tcgtccacc acttctgcg gtgaaaata tcgcaaacg accatcatg cccaacatt cccgggttt cccatgttg atgtgtctc ctgttctgc ccccagatg gagccaaaa ttgacacct gatggtagc cgagtggcc tcaattctg tgcgggttg gggtacaag gaagtcacc caaagagcc aatttcccc atgcatgat atttgtctc gtgcttgat acagatttg cgctctgaa ataattact aagatcaac gcattgagg tactacatg aatgaagct attaaaata atgagccaa attcaagag gagttggca cgacctgaa gaaatgtac acaacccaa cggtttctc aaagatgaa ttatttcat ttgttacat atgagacag caaactgtt gatcgggtt aatgtccgc tatcctgat gtgtggaat aagggcttg gataatatg gagatacta cgcattgac atggacgat gatgaggag gctatatgg tcttatctg tggttcgtc aagtttggc gaagatagg ctctcaaag atgtcgatt aacaaaaat ttggacgaa cttacccaa attgcgaga aggactgcg ccagaacgg gaaatcaag tgcgaactc tgcaatagt gccacccta cgtaatata agtgatgtc cgaattcac ctgttcaga gaggagcac aaatctaat cttgctaaa tatttgaac caaaacaag tag
- >TC014516 RA
caacttttt ccaaaaata aacgaaaac caccgagat ttgaatatt tcacttctt tcttcgtgc ttgcacaac gtgtttgtg gcttttgat atccaccaa gaatgcata aataagtgt gtttgtggt gtggcaatc cacttgttt tctagcaga cagactact ttcggttta ctatagttg aggttatgt tatttttgt tttgaagag ccttatcag ccaaaatga gtgatttac tttccgatt ttttaaacg acaggcttc aacctgtcc ctgtggaag gtgttgtga atggaatca tggattatg aaaaaccag aagaaaata cctacaact cttccgatt ccgaatcag aatcagaca ctgaacaac aagagtaca ttaagaaag aattgagta cctactttc ctgagacag aggcacctt gttctggtg gtctcaccg atatcgagg atggccacg aggagtctg ttctgggaa cagacatca tggaacttc ttcacgttc ctaaagttt ttgaaacct acaaatttg acacttatt acaaaaagg aattaccaa ttgactcgt ctcgtgata aaattcttg acatgatta acactaact ccgttgtaa taattcacg ggcctacag gctgtggta aaactactc aagtgccac agtacattt tggaccact gtagagcca caaaatcgc cttgcaata ttgtagtaa ctcaaccta gaagaatag ctgctataa atattgcac aacgagtgt gcgaagagc gagggtggg ccattggaa cagtttgtg gttatcaag taggtcttg ataaaaacg tcggtgatg atgtgattt taacttata tgacaactg aggttctgt tacaaaaat tgatttccc agaaaaatt tgaacagat ttactcatg tgattatcg atgaagtac acgaacgga gcaaatctc tcgattttt tgcttttga ttgtgagga aatatcttt ttactaact catcttcag ttaagataa ttcttatgt ctgcaacta tggaagcac aagattttg cttactatt ttcgaagta tttcacgca acaaccccc agcagtact tactagctc cggtattac ccgtcacta aaaaaagtg aatacaaag tcagcattt attacaatg aacattttg cttctgcga tgcctccgt acaattttg aagagcctt gcatgcaca aagagcagt acgatgttt gtgccaaat tgattagtc tttttgaca agttggaag aaaatgaga gcaagttga cgcttgccg aacggatta atggaagtg ttttggtgt ttttgccag gttttcatg aaattgaag aaatgcata aagttttag tcagggaga gaaacactt ccgttctcg aatgggaaa ttattccct tgcactcgt ctctggcac aggaacaca tggtcaaag ccttccaga aaccaagac aacgatgca gaaaaataa tcctgtcga ctaatattg ccgaaagtt cggttacag ttcctgatg tcaatttcg tgattgact tctgtctca ccaagaata tgactgtga atgaagtca caaagtttt caagtttaa gtctgcagt gggcttcat atacaaact gcattcaga gggcgggga gagttggcc gtgttgcca atggcagag tgtatcgtg tggtcccca ctagctttt acctccacg aaatgaaac aaactactg ttcctgagc tgcaaagag cgcctttag agaatgtaa ttctgtata tgaagttac tgggattga acgacactc ccaaaaatg ttctttctt tggccctaa gtcctccaa atttgaagg atgtggaac agtgtgtgt ggcacttga aagaagtgg gagctttgc tgcaaactt gtagaggcc accgcactc ccgctgatg gtgacataa ctttcatgg gacgcgtga tgggttcgc ttcccattg acatacatt tgtccaaac ttattctct taggccaca tgtttagtt gcctggatg aagctgtga tcatggctg ctggttgta tgacgaaaa atatattcg ttcaaaact tttacgaca ggttcagga cttacagac agaaacttg tatgggctg atgggtctc atagcgact ttatgattt tattaaatt tgtacaatg tttggctga gtatgaaac gagatagag ccttcagtt cgagccacc aagaaatcg gttggtgta agacgcatt tcgtcaatt tgaaaggac ttagagaat gggatatcc taatccaag agattcact cgcgcttaa aacgactca atatccaaa aattgccag gaccgtcca gtattcctc tgtccattg ttgaaaaac ccatggttc tgaaagtta taatctgtg gcgcattct atccttact atttcataa aatcatcag attttggaa atgtcgacg cgaaagaag ctgtgaaaa ttctcaacg gtcgcgatc cctgcaaca cggtttatt tcactaata tgaaaatga accaaccag gacagattt atgtcagac aaataaaaa aactaatga attgcgaag ataaaccaa acgtccaga ttggattcg atcctcaga gcaccaaag tttttgtcg aatttaaag ccaccagac aaccggaac aagtcacaa ttgatggta gacagtaca tcgctacgg ttgcgagta acatagcgg tggatgtgt acgaagctg tgaggaaga ggcaaatga gagttcctt tcgtgttgc gtattttac cggatagca aggcatggg agtttgcta atatgactc aggcgaaac gacagattg cggaaagtg aagatgtta attgtttca cgactctcg actattcac ctttgccta ccctagata tagaatata tcactgtca ctgtaactc atattattg acgcgggcc acttttatt gtcagaact ggaatgaag aaactcgca tgcttctag accaaattt tcgcggctt tgaacggtc caggagtat tcctagaac ctgccggag agaaaatca aagtcaaca gcgacatat acgcagcgt tgtttaatg aggacggga aattttaca gatgcaaag tcattgact taacacctg gtcaaccaa acgttgcac aggtgtgtt ttatcgatt atgggaacg tgcaaagag ttcccaaaa accgacttt ataaattgc ctgaaaaca gtgagccat gtcgggttc aaccaatcg cgatgtgtt gcgtcctta gcggagtgc aaccagacc ttgtcctaa atcccaaag ctttgtgga gtgaaagtg tcaacaata tcctacgga agaaaacga ctggtgttt tgttaaatg caaaagtat tctcggtgg tagatgagg tcgtgcatc tcgaattat tcctgcaaa atcctggtc ggaattcag tctcgttca atcagtggt tgattaacg aaggactcg gccaaaaat gcgaagaaa gtcaacgat caaaaatgg accacgaaa tgcgtctca aagtccaaa gctctgaag atccaagca acatgagcg cgctcttca acaaaaatc aaattgtga ccagttacg ccgatttcg aagcgcctg aatcgagcg aagccactg aaataatcg aactgaagg gtcctttca gccctcttg agatgaaag tttgcggtc tggttcagg ccagtcagg gggcacctg tgcacgttg atggtgatt cagtcaacg ctgtgatgc tagacgaca atcctgagg attatcacg ccacgttgc tcgttgccg gacaagtct cccagtcgt ccaccactt ctgcggtga aaatatcgc aaacgacca tcatgccca acattcccg ggtttccca tgttgatgt gtctcctgt tctgccccc agatggagc caaaattga cacctgatg gtagccgag tggcctcaa ttctgtgcg ggttggggt acaaggaag tcacccaaa gagccaatt tccccatgc atgatattt gtctcgtgc ttgatacag atttgcgct ctgaaataa ttactaaga tcaacgcat tgaggtact acatgaatg aagctatta aaataatga gccaaattc aagaggagt tggcacgac ctgaagaaa tgtacacaa cccaacggt ttctcaaag atgaattat ttcatttgt tacatatga gacagcaaa ctgttgatc gggttaatg tccgctatc ctgatgtgt ggaataagg gcttggata atatggaga tactacgca ttgacatgg acgatgatg aggaggcta tatggtctt atctgtggt tcgtcaagt ttggcgaag ataggctct caaagatgt cgattaaca aaaatttgg acgaactta cccaaattg cgagaagga ctgcgccag aacgggaaa tcaagtgcg aactctgca atagtgcca ccctacgta atataagtg atgtccgaa ttcacctgt tcagagagg agcacaaat ctaatcttg ctaaatatt tgaaccaaa acaagtagt agatagtgt cgcttttta ttttgttac tttattgtg agtttatta tgttttgta aaagaaagt tttctttgt ttcctgtca ctgtatgaa tctcgataa gtttatatg acgctttta ataaatctt tatttctaa a
