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Gene information
General information
TC014516 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6404679..6414672
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014516
    iBeetle screen iB_10327

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014516 RA
      MSDLLSDFL NDRLQPVPV EGVVNGIMD YEKPEENTY 
      NSSDSESES DTEQQEYIK KELSTYFPE TEAPCSGGL 
      TDIEDGHEE SVLGTDIME LLHVPKVFE TYKFDTYYK 
      KELPIDSSR DKILDMINT NSVVIIHGP TGCGKTTQV 
      PQYILDHCR ATKSPCNIV VTQPRRIAA INIAQRVCE 
      ERGWAIGTV CGYQVGLDK NVGDDVILT YMTTEVLLQ 
      KLISQKNLN RFTHVIIDE VHERSKSLD FLLLIVRKY 
      LFTNSSSVK IILMSATME AQDFAYYFR SISRNNPQQ 
      YLLAPVLPV TKKSEYKVS IYYNEHFAS AMPPYNFEE 
      PCMHKEQYD VCAKLISLF DKLEENESK LTLAERING 
      SVLVFLPGF HEIEEMHKV LVRERNTSV LEWEIIPLH 
      SSLAQEHMV KAFQKPRQR CRKIILSTN IAESSVTVP 
      DVNFVIDFC LTKNMTVNE VTKFSSLSL QWASYTNCI 
      QRAGRVGRV ANGRVYRVV PTSFYLHEM KQTTVPELQ 
      RAPLENVIL YMKLLGLND TPKNVLSLA LSPPNLKDV 
      EQCVWHLKE VGALLQTCR GHRTPADGD ITFMGRVMG 
      SLPIDIHLS KLILLGHMF SCLDEAVIM AAGCMTKNI 
      FVQNFYDRF RTYRQKLVW ADGSHSDFM ILLNLYNVW 
      LSMKRDRAF SSSHQEIGW CKTHFVNLK GLREWDILI 
      QEIHSRLKR LNIQKLPGP SSIPLSIVE KPMVLKVII 
      CGAFYPYYF IKSSDFGNV DAKEAVKIL NGRDPCNTV 
      YFTNMKMNQ PGQIYVRQI KKLMNCEDK PNVQIGFDP 
      QSTKVFVEF KATRQPEQV TIDGRQYIA TVASNIAVD 
      VYEAVRKRQ MRVPFVLRI LPDSKAWEF ANMTQAKRQ 
      IAESEDVNC FTTLDYSPL PTLDIEYIT VTVTHIIDA 
      GHFYCQNWN EETRMLLDQ IFAALNGPG VFLEPAGEK 
      IKVNSDIYA ALFNEDGKF YRCKVIDLT PGQPNVAQV 
      CFIDYGNVQ RVPKNRLYK LPENSEPCR VQPIAMCCV 
      LSGVQPDLV LNPKALWSE SVNNILRKK TTGVLLNAK 
      VFSVVDEVV HLELFLQNP GRNSVSFNQ WLINEGLGQ 
      KCEESQRSK MDHEMRLKV QSSEDPSNM SALFNKNQI 
      VTSYADFEA PESSEATEI IELKGPFSP LEMKVCGLV 
      QASQGAPVH VDGDSVNAV MLDDNPEDY HATLLVAGQ 
      VSQSSTTSA VKISQTTIM PNIPGFPML MCLLFCPQM 
      EPKLTPDGS RVASILCGL GYKEVTQRA NFPMHDICL 
      VLDTDLRSE IITKINALR YYMNEAIKI MSQIQEELA 
      RPEEMYTTQ RFLKDELFH LLHMRQQTV DRVNVRYPD 
      VWNKGLDNM EILRIDMDD DEEAIWSYL WFVKFGEDR 
      LSKMSINKN LDELTQIAR RTAPEREIK CELCNSATL 
      RNISDVRIH LFREEHKSN LAKYLNQNK
    CDS sequence
    • >TC014516 RA
      atgagtgat ttactttcc gatttttta aacgacagg 
      cttcaacct gtccctgtg gaaggtgtt gtgaatgga 
      atcatggat tatgaaaaa ccagaagaa aatacctac 
      aactcttcc gattccgaa tcagaatca gacactgaa 
      caacaagag tacattaag aaagaattg agtacctac 
      tttcctgag acagaggca ccttgttct ggtggtctc 
      accgatatc gaggatggc cacgaggag tctgttctg 
      ggaacagac atcatggaa cttcttcac gttcctaaa 
      gtttttgaa acctacaaa tttgacact tattacaaa 
      aaggaatta ccaattgac tcgtctcgt gataaaatt 
      cttgacatg attaacact aactccgtt gtaataatt 
      cacgggcct acaggctgt ggtaaaact actcaagtg 
      ccacagtac attttggac cactgtaga gccacaaaa 
      tcgccttgc aatattgta gtaactcaa cctagaaga 
      atagctgct ataaatatt gcacaacga gtgtgcgaa 
      gagcgaggg tgggccatt ggaacagtt tgtggttat 
      caagtaggt cttgataaa aacgtcggt gatgatgtg 
      attttaact tatatgaca actgaggtt ctgttacaa 
      aaattgatt tcccagaaa aatttgaac agatttact 
      catgtgatt atcgatgaa gtacacgaa cggagcaaa 
      tctctcgat tttttgctt ttgattgtg aggaaatat 
      ctttttact aactcatct tcagttaag ataattctt 
      atgtctgca actatggaa gcacaagat tttgcttac 
      tattttcga agtatttca cgcaacaac ccccagcag 
      tacttacta gctccggta ttacccgtc actaaaaaa 
      agtgaatac aaagtcagc atttattac aatgaacat 
      tttgcttct gcgatgcct ccgtacaat tttgaagag 
      ccttgcatg cacaaagag cagtacgat gtttgtgcc 
      aaattgatt agtcttttt gacaagttg gaagaaaat 
      gagagcaag ttgacgctt gccgaacgg attaatgga 
      agtgttttg gtgtttttg ccaggtttt catgaaatt 
      gaagaaatg cataaagtt ttagtcagg gagagaaac 
      acttccgtt ctcgaatgg gaaattatt cccttgcac 
      tcgtctctg gcacaggaa cacatggtc aaagccttc 
      cagaaacca agacaacga tgcagaaaa ataatcctg 
      tcgactaat attgccgaa agttcggtt acagttcct 
      gatgtcaat ttcgtgatt gacttctgt ctcaccaag 
      aatatgact gtgaatgaa gtcacaaag ttttcaagt 
      ttaagtctg cagtgggct tcatataca aactgcatt 
      cagagggcg gggagagtt ggccgtgtt gccaatggc 
      agagtgtat cgtgtggtc cccactagc ttttacctc 
      cacgaaatg aaacaaact actgttcct gagctgcaa 
      agagcgcct ttagagaat gtaattctg tatatgaag 
      ttactggga ttgaacgac actcccaaa aatgttctt 
      tctttggcc ctaagtcct ccaaatttg aaggatgtg 
      gaacagtgt gtgtggcac ttgaaagaa gtgggagct 
      ttgctgcaa acttgtaga ggccaccgc actcccgct 
      gatggtgac ataactttc atgggacgc gtgatgggt 
      tcgcttccc attgacata catttgtcc aaacttatt 
      ctcttaggc cacatgttt agttgcctg gatgaagct 
      gtgatcatg gctgctggt tgtatgacg aaaaatata 
      ttcgttcaa aacttttac gacaggttc aggacttac 
      agacagaaa cttgtatgg gctgatggg tctcatagc 
      gactttatg attttatta aatttgtac aatgtttgg 
      ctgagtatg aaacgagat agagccttc agttcgagc 
      caccaagaa atcggttgg tgtaagacg catttcgtc 
      aatttgaaa ggacttaga gaatgggat atcctaatc 
      caagagatt cactcgcgc ttaaaacga ctcaatatc 
      caaaaattg ccaggaccg tccagtatt cctctgtcc 
      attgttgaa aaacccatg gttctgaaa gttataatc 
      tgtggcgca ttctatcct tactatttc ataaaatca 
      tcagatttt ggaaatgtc gacgcgaaa gaagctgtg 
      aaaattctc aacggtcgc gatccctgc aacacggtt 
      tatttcact aatatgaaa atgaaccaa ccaggacag 
      atttatgtc agacaaata aaaaaacta atgaattgc 
      gaagataaa ccaaacgtc cagattgga ttcgatcct 
      cagagcacc aaagttttt gtcgaattt aaagccacc 
      agacaaccg gaacaagtc acaattgat ggtagacag 
      tacatcgct acggttgcg agtaacata gcggtggat 
      gtgtacgaa gctgtgagg aagaggcaa atgagagtt 
      cctttcgtg ttgcgtatt ttaccggat agcaaggca 
      tgggagttt gctaatatg actcaggcg aaacgacag 
      attgcggaa agtgaagat gttaattgt ttcacgact 
      ctcgactat tcacctttg cctacccta gatatagaa 
      tatatcact gtcactgta actcatatt attgacgcg 
      ggccacttt tattgtcag aactggaat gaagaaact 
      cgcatgctt ctagaccaa attttcgcg gctttgaac 
      ggtccagga gtattccta gaacctgcc ggagagaaa 
      atcaaagtc aacagcgac atatacgca gcgttgttt 
      aatgaggac gggaaattt tacagatgc aaagtcatt 
      gacttaaca cctggtcaa ccaaacgtt gcacaggtg 
      tgttttatc gattatggg aacgtgcaa agagttccc 
      aaaaaccga ctttataaa ttgcctgaa aacagtgag 
      ccatgtcgg gttcaacca atcgcgatg tgttgcgtc 
      cttagcgga gtgcaacca gaccttgtc ctaaatccc 
      aaagctttg tggagtgaa agtgtcaac aatatccta 
      cggaagaaa acgactggt gttttgtta aatgcaaaa 
      gtattctcg gtggtagat gaggtcgtg catctcgaa 
      ttattcctg caaaatcct ggtcggaat tcagtctcg 
      ttcaatcag tggttgatt aacgaagga ctcggccaa 
      aaatgcgaa gaaagtcaa cgatcaaaa atggaccac 
      gaaatgcgt ctcaaagtc caaagctct gaagatcca 
      agcaacatg agcgcgctc ttcaacaaa aatcaaatt 
      gtgaccagt tacgccgat ttcgaagcg cctgaatcg 
      agcgaagcc actgaaata atcgaactg aagggtcct 
      ttcagccct cttgagatg aaagtttgc ggtctggtt 
      caggccagt cagggggca cctgtgcac gttgatggt 
      gattcagtc aacgctgtg atgctagac gacaatcct 
      gaggattat cacgccacg ttgctcgtt gccggacaa 
      gtctcccag tcgtccacc acttctgcg gtgaaaata 
      tcgcaaacg accatcatg cccaacatt cccgggttt 
      cccatgttg atgtgtctc ctgttctgc ccccagatg 
      gagccaaaa ttgacacct gatggtagc cgagtggcc 
      tcaattctg tgcgggttg gggtacaag gaagtcacc 
      caaagagcc aatttcccc atgcatgat atttgtctc 
      gtgcttgat acagatttg cgctctgaa ataattact 
      aagatcaac gcattgagg tactacatg aatgaagct 
      attaaaata atgagccaa attcaagag gagttggca 
      cgacctgaa gaaatgtac acaacccaa cggtttctc 
      aaagatgaa ttatttcat ttgttacat atgagacag 
      caaactgtt gatcgggtt aatgtccgc tatcctgat 
      gtgtggaat aagggcttg gataatatg gagatacta 
      cgcattgac atggacgat gatgaggag gctatatgg 
      tcttatctg tggttcgtc aagtttggc gaagatagg 
      ctctcaaag atgtcgatt aacaaaaat ttggacgaa 
      cttacccaa attgcgaga aggactgcg ccagaacgg 
      gaaatcaag tgcgaactc tgcaatagt gccacccta 
      cgtaatata agtgatgtc cgaattcac ctgttcaga 
      gaggagcac aaatctaat cttgctaaa tatttgaac 
      caaaacaag tag
    mRNA sequence
    • >TC014516 RA
      caacttttt ccaaaaata aacgaaaac caccgagat 
      ttgaatatt tcacttctt tcttcgtgc ttgcacaac 
      gtgtttgtg gcttttgat atccaccaa gaatgcata 
      aataagtgt gtttgtggt gtggcaatc cacttgttt 
      tctagcaga cagactact ttcggttta ctatagttg 
      aggttatgt tatttttgt tttgaagag ccttatcag 
      ccaaaatga gtgatttac tttccgatt ttttaaacg 
      acaggcttc aacctgtcc ctgtggaag gtgttgtga 
      atggaatca tggattatg aaaaaccag aagaaaata 
      cctacaact cttccgatt ccgaatcag aatcagaca 
      ctgaacaac aagagtaca ttaagaaag aattgagta 
      cctactttc ctgagacag aggcacctt gttctggtg 
      gtctcaccg atatcgagg atggccacg aggagtctg 
      ttctgggaa cagacatca tggaacttc ttcacgttc 
      ctaaagttt ttgaaacct acaaatttg acacttatt 
      acaaaaagg aattaccaa ttgactcgt ctcgtgata 
      aaattcttg acatgatta acactaact ccgttgtaa 
      taattcacg ggcctacag gctgtggta aaactactc 
      aagtgccac agtacattt tggaccact gtagagcca 
      caaaatcgc cttgcaata ttgtagtaa ctcaaccta 
      gaagaatag ctgctataa atattgcac aacgagtgt 
      gcgaagagc gagggtggg ccattggaa cagtttgtg 
      gttatcaag taggtcttg ataaaaacg tcggtgatg 
      atgtgattt taacttata tgacaactg aggttctgt 
      tacaaaaat tgatttccc agaaaaatt tgaacagat 
      ttactcatg tgattatcg atgaagtac acgaacgga 
      gcaaatctc tcgattttt tgcttttga ttgtgagga 
      aatatcttt ttactaact catcttcag ttaagataa 
      ttcttatgt ctgcaacta tggaagcac aagattttg 
      cttactatt ttcgaagta tttcacgca acaaccccc 
      agcagtact tactagctc cggtattac ccgtcacta 
      aaaaaagtg aatacaaag tcagcattt attacaatg 
      aacattttg cttctgcga tgcctccgt acaattttg 
      aagagcctt gcatgcaca aagagcagt acgatgttt 
      gtgccaaat tgattagtc tttttgaca agttggaag 
      aaaatgaga gcaagttga cgcttgccg aacggatta 
      atggaagtg ttttggtgt ttttgccag gttttcatg 
      aaattgaag aaatgcata aagttttag tcagggaga 
      gaaacactt ccgttctcg aatgggaaa ttattccct 
      tgcactcgt ctctggcac aggaacaca tggtcaaag 
      ccttccaga aaccaagac aacgatgca gaaaaataa 
      tcctgtcga ctaatattg ccgaaagtt cggttacag 
      ttcctgatg tcaatttcg tgattgact tctgtctca 
      ccaagaata tgactgtga atgaagtca caaagtttt 
      caagtttaa gtctgcagt gggcttcat atacaaact 
      gcattcaga gggcgggga gagttggcc gtgttgcca 
      atggcagag tgtatcgtg tggtcccca ctagctttt 
      acctccacg aaatgaaac aaactactg ttcctgagc 
      tgcaaagag cgcctttag agaatgtaa ttctgtata 
      tgaagttac tgggattga acgacactc ccaaaaatg 
      ttctttctt tggccctaa gtcctccaa atttgaagg 
      atgtggaac agtgtgtgt ggcacttga aagaagtgg 
      gagctttgc tgcaaactt gtagaggcc accgcactc 
      ccgctgatg gtgacataa ctttcatgg gacgcgtga 
      tgggttcgc ttcccattg acatacatt tgtccaaac 
      ttattctct taggccaca tgtttagtt gcctggatg 
      aagctgtga tcatggctg ctggttgta tgacgaaaa 
      atatattcg ttcaaaact tttacgaca ggttcagga 
      cttacagac agaaacttg tatgggctg atgggtctc 
      atagcgact ttatgattt tattaaatt tgtacaatg 
      tttggctga gtatgaaac gagatagag ccttcagtt 
      cgagccacc aagaaatcg gttggtgta agacgcatt 
      tcgtcaatt tgaaaggac ttagagaat gggatatcc 
      taatccaag agattcact cgcgcttaa aacgactca 
      atatccaaa aattgccag gaccgtcca gtattcctc 
      tgtccattg ttgaaaaac ccatggttc tgaaagtta 
      taatctgtg gcgcattct atccttact atttcataa 
      aatcatcag attttggaa atgtcgacg cgaaagaag 
      ctgtgaaaa ttctcaacg gtcgcgatc cctgcaaca 
      cggtttatt tcactaata tgaaaatga accaaccag 
      gacagattt atgtcagac aaataaaaa aactaatga 
      attgcgaag ataaaccaa acgtccaga ttggattcg 
      atcctcaga gcaccaaag tttttgtcg aatttaaag 
      ccaccagac aaccggaac aagtcacaa ttgatggta 
      gacagtaca tcgctacgg ttgcgagta acatagcgg 
      tggatgtgt acgaagctg tgaggaaga ggcaaatga 
      gagttcctt tcgtgttgc gtattttac cggatagca 
      aggcatggg agtttgcta atatgactc aggcgaaac 
      gacagattg cggaaagtg aagatgtta attgtttca 
      cgactctcg actattcac ctttgccta ccctagata 
      tagaatata tcactgtca ctgtaactc atattattg 
      acgcgggcc acttttatt gtcagaact ggaatgaag 
      aaactcgca tgcttctag accaaattt tcgcggctt 
      tgaacggtc caggagtat tcctagaac ctgccggag 
      agaaaatca aagtcaaca gcgacatat acgcagcgt 
      tgtttaatg aggacggga aattttaca gatgcaaag 
      tcattgact taacacctg gtcaaccaa acgttgcac 
      aggtgtgtt ttatcgatt atgggaacg tgcaaagag 
      ttcccaaaa accgacttt ataaattgc ctgaaaaca 
      gtgagccat gtcgggttc aaccaatcg cgatgtgtt 
      gcgtcctta gcggagtgc aaccagacc ttgtcctaa 
      atcccaaag ctttgtgga gtgaaagtg tcaacaata 
      tcctacgga agaaaacga ctggtgttt tgttaaatg 
      caaaagtat tctcggtgg tagatgagg tcgtgcatc 
      tcgaattat tcctgcaaa atcctggtc ggaattcag 
      tctcgttca atcagtggt tgattaacg aaggactcg 
      gccaaaaat gcgaagaaa gtcaacgat caaaaatgg 
      accacgaaa tgcgtctca aagtccaaa gctctgaag 
      atccaagca acatgagcg cgctcttca acaaaaatc 
      aaattgtga ccagttacg ccgatttcg aagcgcctg 
      aatcgagcg aagccactg aaataatcg aactgaagg 
      gtcctttca gccctcttg agatgaaag tttgcggtc 
      tggttcagg ccagtcagg gggcacctg tgcacgttg 
      atggtgatt cagtcaacg ctgtgatgc tagacgaca 
      atcctgagg attatcacg ccacgttgc tcgttgccg 
      gacaagtct cccagtcgt ccaccactt ctgcggtga 
      aaatatcgc aaacgacca tcatgccca acattcccg 
      ggtttccca tgttgatgt gtctcctgt tctgccccc 
      agatggagc caaaattga cacctgatg gtagccgag 
      tggcctcaa ttctgtgcg ggttggggt acaaggaag 
      tcacccaaa gagccaatt tccccatgc atgatattt 
      gtctcgtgc ttgatacag atttgcgct ctgaaataa 
      ttactaaga tcaacgcat tgaggtact acatgaatg 
      aagctatta aaataatga gccaaattc aagaggagt 
      tggcacgac ctgaagaaa tgtacacaa cccaacggt 
      ttctcaaag atgaattat ttcatttgt tacatatga 
      gacagcaaa ctgttgatc gggttaatg tccgctatc 
      ctgatgtgt ggaataagg gcttggata atatggaga 
      tactacgca ttgacatgg acgatgatg aggaggcta 
      tatggtctt atctgtggt tcgtcaagt ttggcgaag 
      ataggctct caaagatgt cgattaaca aaaatttgg 
      acgaactta cccaaattg cgagaagga ctgcgccag 
      aacgggaaa tcaagtgcg aactctgca atagtgcca 
      ccctacgta atataagtg atgtccgaa ttcacctgt 
      tcagagagg agcacaaat ctaatcttg ctaaatatt 
      tgaaccaaa acaagtagt agatagtgt cgcttttta 
      ttttgttac tttattgtg agtttatta tgttttgta 
      aaagaaagt tttctttgt ttcctgtca ctgtatgaa 
      tctcgataa gtttatatg acgctttta ataaatctt 
      tatttctaa a
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