Status
Please wait ...
Gene information
General information
TC010592 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 5387719..5394253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010592
    iBeetle screen iB_10317

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010592 RA
      MLPGNVDPR RPEKVQRYK PPTANANGQ GEDLTPDYM 
      NILGQRATT ANASAEATT DKHVVDVTV PTAEAHQVA 
      EEVSVEETN ATNVIPTSP QYGVAIIPT NVTIADLPT 
      ETVTISTDS VIDTTAKPP DDPKDLHST PSRNDRFKV 
      VKIASLEPF KRGRWKCMD YVDEAPPPI TQPSKVTQS 
      TGSISVPAG GVYLQTQSL PQQQIQQML LQSGFTNGT 
      QFFPNVQAQ MIPQTQYFY PPNNLQTQT LPQQLVNTS 
      GVPSSMPAQ FINASQPYF ATGVVQNSG FAIPPNYQN 
      VQYVPANIL QNQGSAFVP TSQTVQLPA NFQQSQTYA 
      GQPNVTAPM VNGHAFPQQ NEQQVTSSS LPTQAAKSV 
      ILNTSVQQP VVSQAQISQ PSIQVVQSS TQSQSVPVI 
      QNQQYQQNT NFQQVPAQN VVQNQSNVV PTTAVFDQN 
      QTTQDKNQA NNVYTNPQF DMNVNSLTV LENQEGAGE 
      ATVSETNEV SSENPDDPS KTNPVVNAI DNKIEQAMD 
      LVKSHLMYT VREEVEVLK EKIAELMEK IQQLETENN 
      FLRSQIPKS QAPGTATPI LTTTSTTTT TATSTAPNE 
      TTPQQQQHM CGLMMRLKW CAWVALYCS CISFANSRL 
      SDDTKQILS SFMLSISAI VMSYLQNPQ PMTPPWASS 
      VN
    CDS sequence
    • >TC010592 RA
      atgcttcct ggtaatgtc gacccccga agacctgag 
      aaagttcaa aggtataag cccccaact gcaaatgct 
      aatggacag ggggaagac ctcacacca gactacatg 
      aacattttg ggtcaacgc gcgaccacg gcaaatgca 
      tcggcggag gctacgact gacaaacac gtggtggat 
      gtaaccgtg ccgacggcg gaggcccac caagtggct 
      gaggaagtt tcagtcgaa gagacgaac gccaccaac 
      gtgataccc accagccct caatacggc gtcgctatc 
      ataccgacc aacgtcacc atcgcggac ttgcccacg 
      gagacggtc acgatatcg accgattcg gtgatcgac 
      accacggcg aagccgccc gacgacccc aaagacctg 
      cattcgacg cccagccgc aacgaccgc ttcaaagtg 
      gtcaaaatc gccagcttg gagccattc aagcgcggc 
      cgctggaaa tgcatggac tacgtcgac gaggcgccg 
      ccccccatc acccagccg agtaaggtg acccagtcc 
      acggggagc atctccgtg cccgccgga ggcgtctac 
      ctccagacc cagagcctg ccccagcag cagatccag 
      cagatgctc ctccagagc ggcttcacc aacggcacg 
      cagtttttt cccaacgtt caagcccaa atgatcccc 
      caaacgcag tatttctac cccccgaac aacctccaa 
      acgcagact ttgccccag cagttagtg aacacgtcg 
      ggcgtgcct agcagcatg ccagcgcag tttataaac 
      gcatcgcaa ccgtatttt gcgacggga gttgtgcaa 
      aactccggc tttgcgatt ccccccaac taccaaaac 
      gttcaatac gtccccgcg aatatttta cagaatcag 
      ggtagtgcg ttcgttccg acctcacag actgtgcaa 
      ttaccagca aatttccaa cagagtcaa acgtacgcg 
      ggacagccg aacgtgacc gcccccatg gtcaacggt 
      cacgcgttt ccccagcaa aacgaacaa caagtcact 
      tcgagcagt ttgccgaca caggccgcc aaaagtgtg 
      atcctaaac acgagcgtg caacagccc gtcgtcagc 
      caagcgcaa atctcgcag cccagcatc caagtggtg 
      caaagcagc actcaaagc caatcagtt cctgtcatt 
      caaaaccaa cagtaccaa caaaacacg aattttcag 
      caagtaccg gcccagaac gtggtccag aaccaaagc 
      aatgtggtg ccaaccact gccgtgttt gaccaaaat 
      caaacgact caagacaag aaccaggcg aataacgtg 
      tacacgaat cctcagttt gatatgaac gttaatagt 
      ttgacggtg ctcgagaac caggaaggc gcaggcgaa 
      gccacagtc agcgaaacc aacgaagtg agttcggag 
      aacccggac gacccctcc aagaccaac ccggtggtc 
      aacgccatc gataataaa atcgagcaa gcgatggat 
      ctggtcaag agccacctg atgtacacg gttcgggag 
      gaggtggaa gtgctcaag gagaaaatc gcagaactg 
      atggaaaag atccaacag ttggagact gaaaataac 
      tttctcagg tcgcagatc cccaagagc caggcgccg 
      gggactgcg accccgatc ttgacgacc acttcgacg 
      acgacgacc acggcaacg agcactgcg ccgaacgaa 
      acgactccg cagcagcag caacacatg tgcgggctt 
      atgatgcgg ctcaagtgg tgcgcgtgg gtggctttg 
      tactgctct tgcatcagt tttgccaac tctcggctg 
      tcggacgac acgaagcag attctgagt tcctttatg 
      ttgtctata tcagccata gtcatgtca tacctccag 
      aacccgcag ccgatgact ccaccatgg gcttcgtcc 
      gtgaattaa
    mRNA sequence
    • >TC010592 RA
      actgtgtta tgacattta ttaattcgc ataggttgt 
      gcttgtcgc cattgtgat ttcttagga ctatatttt 
      agcaaaaag tatcagaat gcttcctgg taatgtcga 
      cccccgaag acctgagaa agttcaaag gtataagcc 
      cccaactgc aaatgctaa tggacaggg ggaagacct 
      cacaccaga ctacatgaa cattttggg tcaacgcgc 
      gaccacggc aaatgcatc ggcggaggc tacgactga 
      caaacacgt ggtggatgt aaccgtgcc gacggcgga 
      ggcccacca agtggctga ggaagtttc agtcgaaga 
      gacgaacgc caccaacgt gatacccac cagccctca 
      atacggcgt cgctatcat accgaccaa cgtcaccat 
      cgcggactt gcccacgga gacggtcac gatatcgac 
      cgattcggt gatcgacac cacggcgaa gccgcccga 
      cgaccccaa agacctgca ttcgacgcc cagccgcaa 
      cgaccgctt caaagtggt caaaatcgc cagcttgga 
      gccattcaa gcgcggccg ctggaaatg catggacta 
      cgtcgacga ggcgccgcc ccccatcac ccagccgag 
      taaggtgac ccagtccac ggggagcat ctccgtgcc 
      cgccggagg cgtctacct ccagaccca gagcctgcc 
      ccagcagca gatccagca gatgctcct ccagagcgg 
      cttcaccaa cggcacgca gttttttcc caacgttca 
      agcccaaat gatccccca aacgcagta tttctaccc 
      cccgaacaa cctccaaac gcagacttt gccccagca 
      gttagtgaa cacgtcggg cgtgcctag cagcatgcc 
      agcgcagtt tataaacgc atcgcaacc gtattttgc 
      gacgggagt tgtgcaaaa ctccggctt tgcgattcc 
      ccccaacta ccaaaacgt tcaatacgt ccccgcgaa 
      tattttaca gaatcaggg tagtgcgtt cgttccgac 
      ctcacagac tgtgcaatt accagcaaa tttccaaca 
      gagtcaaac gtacgcggg acagccgaa cgtgaccgc 
      ccccatggt caacggtca cgcgtttcc ccagcaaaa 
      cgaacaaca agtcacttc gagcagttt gccgacaca 
      ggccgccaa aagtgtgat cctaaacac gagcgtgca 
      acagcccgt cgtcagcca agcgcaaat ctcgcagcc 
      cagcatcca agtggtgca aagcagcac tcaaagcca 
      atcagttcc tgtcattca aaaccaaca gtaccaaca 
      aaacacgaa ttttcagca agtaccggc ccagaacgt 
      ggtccagaa ccaaagcaa tgtggtgcc aaccactgc 
      cgtgtttga ccaaaatca aacgactca agacaagaa 
      ccaggcgaa taacgtgta cacgaatcc tcagtttga 
      tatgaacgt taatagttt gacggtgct cgagaacca 
      ggaaggcgc aggcgaagc cacagtcag cgaaaccaa 
      cgaagtgag ttcggagaa cccggacga cccctccaa 
      gaccaaccc ggtggtcaa cgccatcga taataaaat 
      cgagcaagc gatggatct ggtcaagag ccacctgat 
      gtacacggt tcgggagga ggtggaagt gctcaagga 
      gaaaatcgc agaactgat ggaaaagat ccaacagtt 
      ggagactga aaataactt tctcaggtc gcagatccc 
      caagagcca ggcgccggg gactgcgac cccgatctt 
      gacgaccac ttcgacgac gacgaccac ggcaacgag 
      cactgcgcc gaacgaaac gactccgca gcagcagca 
      acacatgtg cgggcttat gatgcggct caagtggtg 
      cgcgtgggt ggctttgta ctgctcttg catcagttt 
      tgccaactc tcggctgtc ggacgacac gaagcagat 
      tctgagttc ctttatgtt gtctatatc agccatagt 
      catgtcata cctccagaa cccgcagcc gatgactcc 
      accatgggc ttcgtccgt gaattaaaa caaatgttt 
      aaaaacatt caaatttaa taaaatatg tacaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY