Status
Please wait ...
Gene information
General information
TC033643
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009116,TC009115
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4829568..4834675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033643
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_10310

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Fertility
      yolk late sometimesamount decreased - Number of animals showing the phenotype: 2

      ovary without detectable defects - Number of animals showing the phenotype: 2
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033643 RA
        MADGINLSA INKFDGNNY KQWRFQLKC ALKAKGVYS 
        IAIGEIEKP SANRVEELN TWNKKDAIA MCVITSTME 
        LSQITMIES CDTAKEILD KLDAIYDIK TETNKMLIH 
        EEFHQYKMC LNDSIAQHI SKVENLARR IRESGDNIS 
        EVAIITKIL GTLPAKYRN FRQAWLSLA EDKQTLSNL 
        TSRLIDEER NLTTVETTE NALVTTTRV VPNKKTIRG 
        KPRITCYNC NKKGHISRE CRAPRKTHV TQGHSGAFL 
        IEDVNEIAT KIQEDEAWI LDSGASAHM TSRRDMFST 
        IQEVDEFSV KLGNGSELK VKGKGTIEI ECWLENEWI 
        KNKMTDVWY IPNLKRNLF SEGQITKKG MTITKENNK 
        AKIVCDGVV KACAVRQSN NIYKLLIRC TTKTSEVNL 
        TATRSLQTW HQRLGHINM KTIQEMAKR GLIDAHGTT 
        DDEVNQICE GCRYGKQHK MPYHKIEKR NYKAGELIH 
        TDVCGPMSV DSVGGCRFF ILFKDDSTN FRTVYFVKH 
        KSDALDCFK QFYYMCKNK FGHPIKTLR ADNGGEYVN 
        QEFHKFLKD RGIILETCA PYCHEQNGK AERENRTLV 
        ESARSMIFT KNLPLYLWA EAVNTAAYI LNRTTNSIN 
        QEVTPYELW TNKTPDLKH IRIFGSDAY MHVPDNLRK 
        KLEPKSKKL ILVGYDNDS TNYRLFDRA TRKIKIARN 
        VTFGEDNEL TLPRANKIV ITDEIHDDE RNLEHNNQN 
        DQTKEPEEE PVPETEDRR GYNLRPREI LKTPNNLDD 
        YVINIVEGD TPNTYDEAI TSSESEEWR NAIQEELDA 
        LKKNDTWNL VNLPVDKKA IGSKWVFKI KRSPNNKNI 
        RYKARLCAK GFAQTEGVD YNETYAPTT RYDTIRILL 
        AVAARENYQ MIQFDVKTA FLHGELEEN IYMQPPPGL 
        SVPPNSVLK LKKSLYGLK QSPRCWNRK FNEFLELFG 
        LKQCESDKC VYTGKFNEQ LMILLLYVD DGLILAKDM 
        DTLHKMGNK LKSAFEVTI CEPEYFVGL EIKRQSDPK 
        VGKNSIFIH LSNFIDRII DRFNMNDAR TCNTPADPN 
        AILSQSDPN EPKNNDVLF PYREAIGCL MFAAISARP 
        DIMGLRSLG ILYNNSGKL IGYSDADYA NDIDSRKST 
        SGFVFKLGD GAITWCSRR QRCNSLSTT EAEYVAASE 
        ATKEAVWIS GLLSEVGEK CGGVALCVD NQSAIKLVK 
        NPMYHKRTK HIDVRYHFV HSLSAPGSE ERGYATIKS 
        RQLLRTFFQ RPSIKALFE PPLETTFCV EVQIESKSS 
        SGGLEIRSI ESKSLQTSF ECCQKSVQI KLSEEELLE 
        KLNFSETKE EKAEEDGFV ETLSEEEEE EKTEEELEI 
        KNVEKKLTL VFQKLDCVN IESDYSIAK EENSEEIDA 
        LEVSRVKTP KKKTKFCLN FLSCLNCVS DDK
      CDS sequence
      • >TC033643 RA
        atggcagac ggaataaat ttgtcagca ataaacaaa 
        ttcgacgga aacaactac aagcagtgg cggttccag 
        ctaaaatgc gccctaaag gcaaagggg gtgtattca 
        atagcaata ggcgaaata gagaaaccg tcagcgaat 
        agggttgaa gaactcaac acctggaac aaaaaggac 
        gccatagca atgtgcgta ataacatct acgatggaa 
        ttgtctcaa ataacgatg atagaaagc tgcgatacg 
        gctaaagaa attctcgac aagttggat gcgatctac 
        gatataaag acggagaca aacaagatg cttatacac 
        gaggagttt catcagtac aaaatgtgt ttaaatgat 
        tccattgca caacacatt tcaaaagtc gagaatctg 
        gcaagacga ataagagaa tctggcgac aatataagc 
        gaagtggcc ataattaca aaaattctt ggtacgctg 
        ccggcgaaa taccgcaat tttagacag gcatggttg 
        tctttagcg gaagacaaa caaacactt agcaacctg 
        acatcaaga ctcatcgac gaagagcgt aatttaaca 
        actgtggaa acaacagaa aacgcgttg gtcaccact 
        acacgtgtc gtaccaaac aagaaaacg ataagagga 
        aaaccacgc attacgtgc tacaattgt aataaaaaa 
        gggcatatt tcaagagag tgtcgtgcc ccacgaaaa 
        acccatgtg actcaaggt cacagtgga gcattctta 
        atcgaagat gtcaacgaa attgctacc aaaatccaa 
        gaagatgaa gcatggatc ctggacagc ggcgcctcg 
        gcgcatatg acgtcacga cgcgacatg ttttcaaca 
        atacaagag gtcgatgag ttcagtgtg aaacttggc 
        aatggcagc gaactaaaa gtaaaagga aagggtacg 
        attgaaatc gaatgctgg ctagaaaac gaatggatt 
        aaaaacaaa atgacagat gtttggtat attccaaat 
        ctaaagaga aacctcttc tcggagggt caaattacg 
        aaaaaggga atgacaatt acaaaagag aataacaaa 
        gccaaaata gtttgtgat ggtgtcgta aaagcctgt 
        gcagttcgg cagtcaaat aatatttat aaattactg 
        atacgttgt acaacaaaa acaagcgag gttaatttg 
        acagcgaca agaagtcta caaacttgg catcagcgc 
        ttaggacac atcaatatg aaaacaatc caggagatg 
        gcgaagaga ggcttaatc gacgcacat ggaacaact 
        gatgacgag gtcaatcaa atttgcgag ggttgtcgg 
        tatggtaag caacacaaa atgccatat cataaaatc 
        gagaaaaga aattacaaa gccggtgaa ttaattcac 
        acagacgtg tgtggtcca atgtcagta gattccgtc 
        ggcggatgt cgattcttt atacttttc aaagacgat 
        tcgacgaat tttagaacc gtttatttc gtaaaacat 
        aaatcagat gctctcgat tgtttcaaa cagttctat 
        tatatgtgc aaaaataaa tttggtcac ccaattaaa 
        accttgcgt gcggacaat ggcggggaa tacgtgaat 
        caggaattt cataagttc ttaaaagat agaggcata 
        attctcgaa acctgcgcc ccatattgt cacgaacaa 
        aacggaaaa gctgaacgc gaaaatcgg actctagtt 
        gaaagcgcc cgatctatg atctttacg aagaattta 
        ccattatat ttgtgggcg gaagccgta aacacggcg 
        gcatatatt ttgaatcga acgacaaat tcgatcaac 
        caagaagtg acgccatac gaactttgg acaaacaag 
        acacctgat ctaaaacac ataagaatc tttggttct 
        gacgcgtac atgcacgta cctgataac ttaagaaag 
        aaattagag ccgaaaagc aagaaactg atccttgta 
        gggtatgat aatgactcg acaaattac agactgttt 
        gatcgagca actcgaaag attaaaatc gctagaaat 
        gtaacattc ggcgaagat aatgaactg actttaccc 
        agagcaaat aagattgta atcacggat gaaattcac 
        gacgatgaa agaaattta gagcataat aaccaaaac 
        gatcaaact aaagaaccc gaggaagaa cccgtacca 
        gaaactgaa gatcgaaga gggtataat ctgagacca 
        cgtgaaatt ttaaaaaca ccaaacaat cttgatgat 
        tatgtgata aacatagtt gagggcgat accccaaat 
        acatatgac gaagcaatc accagtagc gaatccgaa 
        gagtggaga aatgcaata caggaggaa ctggacgct 
        ttgaagaag aacgatact tggaatctt gtcaaccta 
        ccagttgat aaaaaggca attggatct aagtgggtg 
        ttcaaaatt aaaagatcc cccaataat aaaaatatt 
        cgttataag gcacgatta tgtgcaaaa ggttttgcc 
        cagaccgaa ggcgtagat tataacgaa acgtatgca 
        ccgacaact cgatacgac acgataaga attcttttg 
        gccgtcgcc gcaagagaa aattatcag atgatacag 
        ttcgacgtc aaaacagca ttccttcac ggagaatta 
        gaagaaaac atttacatg caacctcct cccgggtta 
        tctgtgcca cctaattct gtgttaaaa ttaaaaaag 
        tcattgtac ggacttaag caatcacct cgctgctgg 
        aacaggaaa ttcaacgag ttcctcgaa cttttcgga 
        cttaaacaa tgtgaaagt gataaatgt gtttacaca 
        ggaaaattc aacgaacaa ttaatgatc cttctactg 
        tatgtagat gatggtcta attttggca aaggacatg 
        gacactcta cacaaaatg ggcaacaaa ttaaaaagt 
        gcgttcgaa gtgaccatt tgtgaacca gaatatttt 
        gtgggactg gaaattaaa agacagagt gatcctaaa 
        gttggtaag aattcgatc ttcatacat ttatcgaac 
        tttatcgat cgtattatt gaccgtttt aatatgaac 
        gatgcgaga acatgtaat acaccagct gatccaaac 
        gcgatattg tcgcaatcc gatccaaac gaacccaag 
        aacaatgac gtgctattt ccttaccga gaagcaatt 
        ggatgttta atgttcgcc gcaatttcc gcgcgtccc 
        gatattatg ggactaagg agcttggga atcctctac 
        aacaactcc ggtaaactc atcggatac tcggatgcc 
        gattatgcg aacgacatc gattccagg aagtcgaca 
        agcggcttc gttttcaaa ctgggagac ggcgccatc 
        acctggtgt agtcgtcgt caaaggtgc aactcccta 
        tctacaacg gaggccgag tacgtggca gcttcggaa 
        gctaccaag gaagcagtg tggataagt ggcctctta 
        agtgaagtg ggtgaaaag tgcggtggt gtcgctttg 
        tgcgttgac aaccaatca gcgattaag cttgttaaa 
        aatccaatg tatcataaa cgcactaag catatcgat 
        gtccgatat cactttgta cattctcta agtgccccc 
        ggatctgag gaacgtggc tatgccaca atcaagtcg 
        cgtcagctt ttgcgcact tttttccaa agaccgtcg 
        ataaaagcg ctttttgag ccgccgctc gaaacgacg 
        ttctgtgtc gaagtgcaa attgagtcc aaaagctca 
        agcggtggg cttgaaata cgttccatc gaaagtaag 
        tcgttgcaa acatcgttc gaatgttgc caaaaatcg 
        gttcagatt aaactgtcc gaagaagag ttacttgaa 
        aagctcaat ttcagcgag acgaaggag gaaaaggct 
        gaagaagac ggtttcgtc gagactctc agtgaagag 
        gaagaagaa gaaaagacg gaagaagag ttagaaata 
        aagaatgta gagaaaaag ttgactcta gtgttccaa 
        aaactggac tgtgtcaat attgagtct gattattca 
        atcgccaag gaggagaat agcgaggaa attgatgct 
        ttagaagtg tctcgcgtt aaaacgcca aaaaagaag 
        accaaattt tgtctgaat ttcttgtcc tgccttaat 
        tgcgtgtct gacgataaa taa
      mRNA sequence
      • >TC033643 RA
        ccaattctt tcctgcccg ttccattaa tatattaac 
        cgtattatc aaccgtgtt tcaatacta cctaccact 
        gctcgacca ccaccacaa caggttatg ggcccaggt 
        cgcaagggt agttttagt tcagttttg tctacagta 
        acgttcgag tgaaacacg cgttcgggc gattaaaaa 
        ctggttcgt gcggtcaaa accagtgtg tcgggtgat 
        tcacagaca aaaacgagt gtcgtgaaa acaagaatg 
        gcagacgga ataaatttg tcagcaata aacaaattc 
        gacggaaac aactacaag cagtggcgg ttccagcta 
        aaatgcgcc ctaaaggca aagggggtg tattcaata 
        gcaataggc gaaatagag aaaccgtca gcgaatagg 
        gttgaagaa ctcaacacc tggaacaaa aaggacgcc 
        atagcaatg tgcgtaata acatctacg atggaattg 
        tctcaaata acgatgata gaaagctgc gatacggct 
        aaagaaatt ctcgacaag ttggatgcg atctacgat 
        ataaagacg gagacaaac aagatgctt atacacgag 
        gagtttcat cagtacaaa atgtgttta aatgattcc 
        attgcacaa cacatttca aaagtcgag aatctggca 
        agacgaata agagaatct ggcgacaat ataagcgaa 
        gtggccata attacaaaa attcttggt acgctgccg 
        gcgaaatac cgcaatttt agacaggca tggttgtct 
        ttagcggaa gacaaacaa acacttagc aacctgaca 
        tcaagactc atcgacgaa gagcgtaat ttaacaact 
        gtggaaaca acagaaaac gcgttggtc accactaca 
        cgtgtcgta ccaaacaag aaaacgata agaggaaaa 
        ccacgcatt acgtgctac aattgtaat aaaaaaggg 
        catatttca agagagtgt cgtgcccca cgaaaaacc 
        catgtgact caaggtcac agtggagca ttcttaatc 
        gaagatgtc aacgaaatt gctaccaaa atccaagaa 
        gatgaagca tggatcctg gacagcggc gcctcggcg 
        catatgacg tcacgacgc gacatgttt tcaacaata 
        caagaggtc gatgagttc agtgtgaaa cttggcaat 
        ggcagcgaa ctaaaagta aaaggaaag ggtacgatt 
        gaaatcgaa tgctggcta gaaaacgaa tggattaaa 
        aacaaaatg acagatgtt tggtatatt ccaaatcta 
        aagagaaac ctcttctcg gagggtcaa attacgaaa 
        aagggaatg acaattaca aaagagaat aacaaagcc 
        aaaatagtt tgtgatggt gtcgtaaaa gcctgtgca 
        gttcggcag tcaaataat atttataaa ttactgata 
        cgttgtaca acaaaaaca agcgaggtt aatttgaca 
        gcgacaaga agtctacaa acttggcat cagcgctta 
        ggacacatc aatatgaaa acaatccag gagatggcg 
        aagagaggc ttaatcgac gcacatgga acaactgat 
        gacgaggtc aatcaaatt tgcgagggt tgtcggtat 
        ggtaagcaa cacaaaatg ccatatcat aaaatcgag 
        aaaagaaat tacaaagcc ggtgaatta attcacaca 
        gacgtgtgt ggtccaatg tcagtagat tccgtcggc 
        ggatgtcga ttctttata cttttcaaa gacgattcg 
        acgaatttt agaaccgtt tatttcgta aaacataaa 
        tcagatgct ctcgattgt ttcaaacag ttctattat 
        atgtgcaaa aataaattt ggtcaccca attaaaacc 
        ttgcgtgcg gacaatggc ggggaatac gtgaatcag 
        gaatttcat aagttctta aaagataga ggcataatt 
        ctcgaaacc tgcgcccca tattgtcac gaacaaaac 
        ggaaaagct gaacgcgaa aatcggact ctagttgaa 
        agcgcccga tctatgatc tttacgaag aatttacca 
        ttatatttg tgggcggaa gccgtaaac acggcggca 
        tatattttg aatcgaacg acaaattcg atcaaccaa 
        gaagtgacg ccatacgaa ctttggaca aacaagaca 
        cctgatcta aaacacata agaatcttt ggttctgac 
        gcgtacatg cacgtacct gataactta agaaagaaa 
        ttagagccg aaaagcaag aaactgatc cttgtaggg 
        tatgataat gactcgaca aattacaga ctgtttgat 
        cgagcaact cgaaagatt aaaatcgct agaaatgta 
        acattcggc gaagataat gaactgact ttacccaga 
        gcaaataag attgtaatc acggatgaa attcacgac 
        gatgaaaga aatttagag cataataac caaaacgat 
        caaactaaa gaacccgag gaagaaccc gtaccagaa 
        actgaagat cgaagaggg tataatctg agaccacgt 
        gaaatttta aaaacacca aacaatctt gatgattat 
        gtgataaac atagttgag ggcgatacc ccaaataca 
        tatgacgaa gcaatcacc agtagcgaa tccgaagag 
        tggagaaat gcaatacag gaggaactg gacgctttg 
        aagaagaac gatacttgg aatcttgtc aacctacca 
        gttgataaa aaggcaatt ggatctaag tgggtgttc 
        aaaattaaa agatccccc aataataaa aatattcgt 
        tataaggca cgattatgt gcaaaaggt tttgcccag 
        accgaaggc gtagattat aacgaaacg tatgcaccg 
        acaactcga tacgacacg ataagaatt cttttggcc 
        gtcgccgca agagaaaat tatcagatg atacagttc 
        gacgtcaaa acagcattc cttcacgga gaattagaa 
        gaaaacatt tacatgcaa cctcctccc gggttatct 
        gtgccacct aattctgtg ttaaaatta aaaaagtca 
        ttgtacgga cttaagcaa tcacctcgc tgctggaac 
        aggaaattc aacgagttc ctcgaactt ttcggactt 
        aaacaatgt gaaagtgat aaatgtgtt tacacagga 
        aaattcaac gaacaatta atgatcctt ctactgtat 
        gtagatgat ggtctaatt ttggcaaag gacatggac 
        actctacac aaaatgggc aacaaatta aaaagtgcg 
        ttcgaagtg accatttgt gaaccagaa tattttgtg 
        ggactggaa attaaaaga cagagtgat cctaaagtt 
        ggtaagaat tcgatcttc atacattta tcgaacttt 
        atcgatcgt attattgac cgttttaat atgaacgat 
        gcgagaaca tgtaataca ccagctgat ccaaacgcg 
        atattgtcg caatccgat ccaaacgaa cccaagaac 
        aatgacgtg ctatttcct taccgagaa gcaattgga 
        tgtttaatg ttcgccgca atttccgcg cgtcccgat 
        attatggga ctaaggagc ttgggaatc ctctacaac 
        aactccggt aaactcatc ggatactcg gatgccgat 
        tatgcgaac gacatcgat tccaggaag tcgacaagc 
        ggcttcgtt ttcaaactg ggagacggc gccatcacc 
        tggtgtagt cgtcgtcaa aggtgcaac tccctatct 
        acaacggag gccgagtac gtggcagct tcggaagct 
        accaaggaa gcagtgtgg ataagtggc ctcttaagt 
        gaagtgggt gaaaagtgc ggtggtgtc gctttgtgc 
        gttgacaac caatcagcg attaagctt gttaaaaat 
        ccaatgtat cataaacgc actaagcat atcgatgtc 
        cgatatcac tttgtacat tctctaagt gcccccgga 
        tctgaggaa cgtggctat gccacaatc aagtcgcgt 
        cagcttttg cgcactttt ttccaaaga ccgtcgata 
        aaagcgctt tttgagccg ccgctcgaa acgacgttc 
        tgtgtcgaa gtgcaaatt gagtccaaa agctcaagc 
        ggtgggctt gaaatacgt tccatcgaa agtaagtcg 
        ttgcaaaca tcgttcgaa tgttgccaa aaatcggtt 
        cagattaaa ctgtccgaa gaagagtta cttgaaaag 
        ctcaatttc agcgagacg aaggaggaa aaggctgaa 
        gaagacggt ttcgtcgag actctcagt gaagaggaa 
        gaagaagaa aagacggaa gaagagtta gaaataaag 
        aatgtagag aaaaagttg actctagtg ttccaaaaa 
        ctggactgt gtcaatatt gagtctgat tattcaatc 
        gccaaggag gagaatagc gaggaaatt gatgcttta 
        gaagtgtct cgcgttaaa acgccaaaa aagaagacc 
        aaattttgt ctgaatttc ttgtcctgc cttaattgc 
        gtgtctgac gataaataa atttctgtc acaaacgta 
        caaaactct ttattca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY