Status
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Gene information
General information
TC033638
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009123
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4713140..4719727
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033638
    iBeetle screen iB_10303

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033638 RA
      MPTSSSAAT IEKKRAFRL QTHVKSIER HDAERSDFL 
      TIESALPSN ERRLTKRSH STLNASSIS RAHSSKLIP 
      CDPITLQTH ADGSVHVTR TQSEKDCSP DRISLDRRG 
      LTAFPIIDG EMKLRLLSL QHNLISNLE GLQAQNFPY 
      LVFLDIYDN QLEQMGCLD TLDNLRVLL VGKNRLRKI 
      EGLDTLKKI EVLDLHGNQ ITHVNGLSC LNELKVLNL 
      AGNQIRYIG VGDFQGLTS LQELNLRRN RLRKLLGFG 
      ETPNLSKLF ISNNDLQSV EDISSLAKS SNLKEISID 
      NNPVFLGGD CVSFLVSYL PQLNKLNSM QITDQVRKA 
      AMAWRRNKE STNSAFMDL TSDVCLNVR REEIISNAR 
      TNWELLRSQ TKCLTAKSS RNLSKNVNL SLDSDLILT 
      SFGKTKAKT YASITTKVP LFTNKKLIR TSSQDTDNS 
      LSSSNASSN EFIRLPPIL VPIIKKMEQ KSEAGSKEN 
      SIKLSGSLS SIGPNVDSS VSSLGSENE SSESSETEV 
      ETEPEEQPK PAEEEKQVP SPRPVVTEK EEPKNVCVA 
      ETGSNASTN TAPSTSGSD QSAKVEKTR NIKSAVNSR 
      TAASRWHNR AATAKPKKQ SPSVTTGGK DREQGGDYL 
      IEICGRYLN IYGQCALRF IDRPWNASK ANDVNSVKF 
      NYVNFNGIT GILNKLKLR FPNIDNFFF KETNIHCLG 
      QINALAEVQ GLISLNIDP EGNPITQKN WRSYAIYRL 
      SHWGLSVIN EVEVTEEEV KKANEEYQS LSDLVLWSL 
      PDVLLQPLL VRLRLDVSY GVSEQNAKK WLLAADPDL 
      RSVVSKEAL QWKKGCGED VAVRQKAKQ YISQLLEEM 
      GNSVNKLRL LDKKWPSIL HEFVRNALL DYSQLDMYM 
      KQKILELK
    CDS sequence
    • >TC033638 RA
      atgccaacc agcagtagt gctgcaacc atcgagaag 
      aagagggct ttccgttta caaacccac gtaaaaagc 
      attgaaaga cacgatgcc gaacgctct gactttctc 
      actattgag tcggcattg ccctcaaac gagcgcaga 
      ttgaccaag cgctcccac agtacttta aacgcgtca 
      agcatttcg agggcgcac agctcgaag ctgatccca 
      tgcgacccc atcacctta caaacgcac gccgatggc 
      tccgtccat gtcacacgc acccaaagc gagaaagac 
      tgcagtcct gatcggata agtctggac aggagaggg 
      ctcaccgcc tttcccatc atcgacggc gaaatgaaa 
      ctcagacta ttatccttg caacataac ttaatcagc 
      aatttagag gggctacaa gcccagaat tttccctac 
      ttagtattt ctagacatc tacgacaac cagttagaa 
      caaatgggc tgcttggac accttggac aatctcagg 
      gtgttgcta gtggggaaa aataggttg cgcaaaatc 
      gagggctta gacacactg aaaaaaatc gaagtgctg 
      gacttgcac ggaaaccaa atcacgcat gttaatggt 
      ttgagttgc ttgaacgag ttgaaagtg ctgaatttg 
      gcggggaat cagattcgg tatatcgga gttggggat 
      ttccaaggc ttgacgtct ttgcaggaa ttaaacttg 
      cgcaggaac cgtcttaga aagttgctg gggttcggg 
      gaaaccccg aatttgagc aagctgttc atcagcaat 
      aatgacttg caaagcgtt gaggacata agcagcttg 
      gcaaaatcg tccaacttg aaggagatc tccatcgac 
      aacaaccct gtgttcctg ggaggcgat tgtgtctca 
      tttttggtt tcttacctc cctcaatta aacaaacta 
      aactcgatg caaattacc gaccaagtg cggaaagct 
      gcaatggca tggcggcga aacaaagag tccacaaac 
      tcggctttt atggacttg accagcgac gtgtgtttg 
      aatgttcgt cgagaggag attatttca aacgcccga 
      accaactgg gagctcctc cgctcccag accaaatgt 
      ttaacagca aaatcgtcg agaaattta tcaaaaaac 
      gtgaacttg tctttagac tccgaccta attttgacg 
      tcttttgga aaaacgaaa gcaaagact tacgccagt 
      attaccacc aaagtgcct ctttttaca aataagaaa 
      ctaataaga accagctcg caagatacc gacaattcg 
      ctctcctca tccaacgcc tcaagtaac gagttcata 
      agactaccc ccgattttg gtcccgatc atcaaaaaa 
      atggagcag aaaagcgaa gcgggctcg aaggagaac 
      agcataaaa ttatccgga agtctctcc agcattggc 
      ccgaacgtc gacagttcg gtcagttcg ctcggaagc 
      gagaacgaa tcgtccgaa agttccgaa acggaagtg 
      gagacggag ccagaggag caaccgaag cccgcggaa 
      gaagagaaa caagtgcca tcgccgagg ccggttgta 
      accgagaaa gaggagccg aaaaatgtg tgtgttgcg 
      gaaactgga agtaatgct tcgacgaac actgcaccc 
      agcacgtca ggtagtgac cagtcggcg aaagttgag 
      aaaacaagg aatattaaa agtgcagtg aatagtcgg 
      acggcagcg tcgaggtgg cataacagg gcggccact 
      gcgaagccc aaaaagcag tcgccgtcg gttacaacc 
      ggggggaag gacagggag caagggggc gattacctg 
      atcgaaatc tgcggcaga tacttgaac atttacggt 
      caatgtgcc ttacgtttt atcgaccgt ccctggaac 
      gcctcaaag gccaacgac gtcaactcg gtcaaattc 
      aactacgtc aacttcaac ggaatcacc ggcattcta 
      aacaagcta aaattacga ttccccaac atcgacaac 
      ttcttcttc aaagagacc aacatccac tgtttgggc 
      caaatcaac gctctggcc gaagtccaa ggcctaatc 
      tcgctcaac atcgaccct gaggggaac cccataacg 
      cagaaaaac tggcgaagc tacgccatc tacagactc 
      tcccactgg ggcttgtct gtgatcaac gaagtggaa 
      gtgaccgaa gaagaagtg aaaaaggcc aacgaggag 
      taccaaagt ttgagcgac ttggtcctg tggtcgttg 
      cccgacgtc ctcctgcag ccgcttcta gtgaggctg 
      aggctggac gtgagttat ggggtttcg gagcagaac 
      gcgaaaaag tggcttttg gcggcggac cccgatttg 
      aggagcgtt gtgagcaag gaggcgctg cagtggaag 
      aaaggatgc ggggaggat gttgcggtt agacagaaa 
      gccaagcaa tatatttcg cagctgttg gaggaaatg 
      ggcaattcg gtgaacaaa ctgcgattg ttggataaa 
      aagtggcct tcgattttg cacgaattt gtgcgaaat 
      gcccttctg gactactca cagttagac atgtacatg 
      aaacagaaa atcttagaa ctgaagtaa
    mRNA sequence
    • >TC033638 RA
      cgacagcga cgtttgtgt ttacaattt ttttctttc 
      caaattttc gcaatgaaa ctcactaca aatgatttc 
      ggtcttggg tcataaatg ccaaccagc agtagtgct 
      gcaaccatc gagaagaag agggctttc cgtttacaa 
      acccacgta aaaagcatt gaaagacac gatgccgaa 
      cgctctgac tttctcact attgagtcg gcattgccc 
      tcaaacgag cgcagattg accaagcgc tcccacagt 
      actttaaac gcgtcaagc atttcgagg gcgcacagc 
      tcgaagctg atcccatgc gaccccatc accttacaa 
      acgcacgcc gatggctcc gtccatgtc acacgcacc 
      caaagcgag aaagactgc agtcctgat cggataagt 
      ctggacagg agagggctc accgccttt cccatcatc 
      gacggcgaa atgaaactc agactatta tccttgcaa 
      cataactta atcagcaat ttagagggg ctacaagcc 
      cagaatttt ccctactta gtatttcta gacatctac 
      gacaaccag ttagaacaa atgggctgc ttggacacc 
      ttggacaat ctcagggtg ttgctagtg gggaaaaat 
      aggttgcgc aaaatcgag ggcttagac acactgaaa 
      aaaatcgaa gtgctggac ttgcacgga aaccaaatc 
      acgcatgtt aatggtttg agttgcttg aacgagttg 
      aaagtgctg aatttggcg gggaatcag attcggtat 
      atcggagtt ggggatttc caaggcttg acgtctttg 
      caggaatta aacttgcgc aggaaccgt cttagaaag 
      ttgctgggg ttcggggaa accccgaat ttgagcaag 
      ctgttcatc agcaataat gacttgcaa agcgttgag 
      gacataagc agcttggca aaatcgtcc aacttgaag 
      gagatctcc atcgacaac aaccctgtg ttcctggga 
      ggcgattgt gtctcattt ttggtttct tacctccct 
      caattaaac aaactaaac tcgatgcaa attaccgac 
      caagtgcgg aaagctgca atggcatgg cggcgaaac 
      aaagagtcc acaaactcg gcttttatg gacttgacc 
      agcgacgtg tgtttgaat gttcgtcga gaggagatt 
      atttcaaac gcccgaacc aactgggag ctcctccgc 
      tcccagacc aaatgttta acagcaaaa tcgtcgaga 
      aatttatca aaaaacgtg aacttgtct ttagactcc 
      gacctaatt ttgacgtct tttggaaaa acgaaagca 
      aagacttac gccagtatt accaccaaa gtgcctctt 
      tttacaaat aagaaacta ataagaacc agctcgcaa 
      gataccgac aattcgctc tcctcatcc aacgcctca 
      agtaacgag ttcataaga ctacccccg attttggtc 
      ccgatcatc aaaaaaatg gagcagaaa agcgaagcg 
      ggctcgaag gagaacagc ataaaatta tccggaagt 
      ctctccagc attggcccg aacgtcgac agttcggtc 
      agttcgctc ggaagcgag aacgaatcg tccgaaagt 
      tccgaaacg gaagtggag acggagcca gaggagcaa 
      ccgaagccc gcggaagaa gagaaacaa gtgccatcg 
      ccgaggccg gttgtaacc gagaaagag gagccgaaa 
      aatgtgtgt gttgcggaa actggaagt aatgcttcg 
      acgaacact gcacccagc acgtcaggt agtgaccag 
      tcggcgaaa gttgagaaa acaaggaat attaaaagt 
      gcagtgaat agtcggacg gcagcgtcg aggtggcat 
      aacagggcg gccactgcg aagcccaaa aagcagtcg 
      ccgtcggtt acaaccggg gggaaggac agggagcaa 
      gggggcgat tacctgatc gaaatctgc ggcagatac 
      ttgaacatt tacggtcaa tgtgcctta cgttttatc 
      gaccgtccc tggaacgcc tcaaaggcc aacgacgtc 
      aactcggtc aaattcaac tacgtcaac ttcaacgga 
      atcaccggc attctaaac aagctaaaa ttacgattc 
      cccaacatc gacaacttc ttcttcaaa gagaccaac 
      atccactgt ttgggccaa atcaacgct ctggccgaa 
      gtccaaggc ctaatctcg ctcaacatc gaccctgag 
      gggaacccc ataacgcag aaaaactgg cgaagctac 
      gccatctac agactctcc cactggggc ttgtctgtg 
      atcaacgaa gtggaagtg accgaagaa gaagtgaaa 
      aaggccaac gaggagtac caaagtttg agcgacttg 
      gtcctgtgg tcgttgccc gacgtcctc ctgcagccg 
      cttctagtg aggctgagg ctggacgtg agttatggg 
      gtttcggag cagaacgcg aaaaagtgg cttttggcg 
      gcggacccc gatttgagg agcgttgtg agcaaggag 
      gcgctgcag tggaagaaa ggatgcggg gaggatgtt 
      gcggttaga cagaaagcc aagcaatat atttcgcag 
      ctgttggag gaaatgggc aattcggtg aacaaactg 
      cgattgttg gataaaaag tggccttcg attttgcac 
      gaatttgtg cgaaatgcc cttctggac tactcacag 
      ttagacatg tacatgaaa cagaaaatc ttagaactg 
      aagtaatta ttggtccac tgccgtttg aaaacactg 
      ttgcactat tgtgaacgt tgaattttt attatgtat 
      ttgttgaat aaatttgtt attctttac tagcccatt 
      gtttcattg tgtaacaca ttttccgcg acaagtcaa 
      ttaaaactt ttcttttct gttcgaagc cattacgta 
      tatgtggca aaagttgga ccgatgaaa gagcaacgt 
      gtttattta tgtaacttc attgaatat taatctctg 
      cctttgaaa atgcgtgat cttgaccgt aattgttgt 
      ttacaacga atttcgaaa cagtcggaa tgcagcgaa 
      taagtgaac gtaatctgc attacttct tgcattcag 
      ttcctcttt tccactgaa tttctcttt aagcaagtc 
      gctaacctt ttccattgt gaaaaaaaa aaacgctta 
      ttaattgcg ctttgattt ttgcaccca gttgtgtct 
      ccaatgtaa cggattgag tcagcttcg tgtttttta 
      aatagctga aatttataa ggatcggga tttgtaatg 
      cggacacat taataattt gaaatgaaa gggagagcc 
      aattatagt ttctattgc tccggtatt atatgcaat 
      aattgacgg aatgcaaca gtaatgtta cctagttta 
      cattttcga aaatgatta tttgtcaga tatgcgcaa 
      cttgcacaa cttgacggg taccaccca aacacgtgg 
      tggtatggt aggttttgc tgaacatgt ggagaaata 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY