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Gene information
General information
TC009180 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3364216..3366124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009180
    iBeetle screen iB_10274

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009180 RA
      MQDQQFRNV KSQGFIFKM MLKGLALLS ILGIVSAAT 
      NKVVCYHGI WSTYRLNNG KFTVEDIDP TLCTHLIYS 
      FVGLGDDSR IKHLEPNLD VNQGNLKKF NALKLKNPN 
      LKTLVAIGG WNEGSVNYS KMAASSSLR AVFIKSVVE 
      FVKTWGFDG FDLDWEYPG QRGGAYNDK SNYVTLIKE 
      MRKEFDKNG LILTAAVAA ASGSVDISY DVPALSKYL 
      DIINVMAYD LRGSWDGVT GHHSGLYPS AVDTTTNQK 
      LLTVDAAIR GWIQRGADP QKIALGLPV YGKTFTLSS 
      ASNAKLGAP VKGAGNSGK YTGEAGMLG YNEIVELQK 
      EGGWKVVWD DTQKNTHMY KGDQWVAFD SPKAISNKV 
      EYAKSLNLG GVMIWSIET DDFRGVSGT KYPILKAIH 
      QTLGGSSNE IVEPVPQPV EEVTQKPSS TKAPAPPSR 
      DLTTTLCTK AGYVRDPDD CSIFYYCLA YNGGFVPLE 
      QRCNAGLVF DEEKLWCDY PEVVKC
    CDS sequence
    • >TC009180 RA
      atgcaagac caacagttt agaaacgtg aagtcgcag 
      ggttttatt ttcaaaatg atgcttaaa ggcctcgcc 
      ctcctcagc atccttgga attgtctca gctgcgacc 
      aataaagtg gtctgttat cacggaata tggtccact 
      tatcggctc aacaacggc aaattcaca gtcgaagac 
      atcgatcca actctttgc acccattta atttactct 
      tttgttgga ttaggagac gatagtcgg attaaacac 
      cttgaaccg aatctggac gtcaatcaa ggcaattta 
      aaaaaattc aatgctttg aaattgaaa aacccaaat 
      ttgaaaact ttggttgcg attggtggc tggaatgaa 
      ggatctgta aactactcc aaaatggcg gcgagttcc 
      agtttaaga gccgtcttc atcaagagt gtggtggag 
      tttgtcaag acatggggg tttgacggg tttgacctt 
      gactgggag tatccaggg caacggggc ggagcttat 
      aatgataaa agcaattat gtcactctg atcaaggaa 
      atgcgcaag gaatttgac aaaaatggg ttgatttta 
      accgccgct gtggccgcc gccagcggt tccgttgac 
      atttcctat gacgtcccc gctttgtct aaatatctg 
      gatattatc aatgttatg gcttatgac ctgagaggg 
      tcgtgggat ggggttacg ggtcaccat tccgggttg 
      tacccatcg gcggtggac accaccacc aaccagaaa 
      ctgttgaca gtggatgcc gccatccgg gggtggatt 
      caaaggggg gctgatccg caaaaaatc gctttaggg 
      ttgcctgtt tacggcaaa acattcaca ctttcgagt 
      gcgagtaat gcgaaactg ggggctccg gtcaagggg 
      gctgggaat tcggggaag tacacgggg gaggcggga 
      atgttggga tacaatgag atcgtcgag ttgcagaaa 
      gagggtgga tggaaggtt gtgtgggac gacacccag 
      aaaaacacc catatgtat aaaggggac caatgggtt 
      gcatttgac agtccgaaa gcaatttct aacaaagtc 
      gagtatgcc aagtccttg aatctagga ggtgtgatg 
      atttggtcc attgaaacc gacgacttt agaggcgtc 
      agtggtacc aagtacccc atcctcaag gccatacac 
      caaacactt ggaggttct agcaatgaa attgtggaa 
      cctgttccc caacccgtc gaagaagtg acccagaaa 
      ccatcctcg accaaagca ccagcaccc ccatctcgc 
      gatttgact acaactctt tgtactaaa gctggttat 
      gtccgagat ccagatgat tgttccatc ttttactat 
      tgtttggct tacaatgga ggttttgtt cctttggaa 
      cagagatgt aacgccggg ttggtgttt gatgaggaa 
      aaattgtgg tgtgattat cccgaagtt gtaaagtgt 
      tga
    mRNA sequence
    • >TC009180 RA
      accgattaa aatttacaa acacactta tttaaaacg 
      aactctcat catgcaaga ccaacagtt tagaaacgt 
      gaagtcgca gggttttat tttcaaaat gatgcttaa 
      aggcctcgc cctcctcag catccttgg aattgtctc 
      agctgcgac caataaagt ggtctgtta tcacggaat 
      atggtccac ttatcggct caacaacgg caaattcac 
      agtcgaaga catcgatcc aactctttg cacccattt 
      aatttactc ttttgttgg attaggaga cgatagtcg 
      gattaaaca ccttgaacc gaatctgga cgtcaatca 
      aggcaattt aaaaaaatt caatgcttt gaaattgaa 
      aaacccaaa tttgaaaac tttggttgc gattggtgg 
      ctggaatga aggatctgt aaactactc caaaatggc 
      ggcgagttc cagtttaag agccgtctt catcaagag 
      tgtggtgga gtttgtcaa gacatgggg gtttgacgg 
      gtttgacct tgactggga gtatccagg gcaacgggg 
      cggagctta taatgataa aagcaatta tgtcactct 
      gatcaagga aatgcgcaa ggaatttga caaaaatgg 
      gttgatttt aaccgccgc tgtggccgc cgccagcgg 
      ttccgttga catttccta tgacgtccc cgctttgtc 
      taaatatct ggatattat caatgttat ggcttatga 
      cctgagagg gtcgtggga tggggttac gggtcacca 
      ttccgggtt gtacccatc ggcggtgga caccaccac 
      caaccagaa actgttgac agtggatgc cgccatccg 
      ggggtggat tcaaagggg ggctgatcc gcaaaaaat 
      cgctttagg gttgcctgt ttacggcaa aacattcac 
      actttcgag tgcgagtaa tgcgaaact gggggctcc 
      ggtcaaggg ggctgggaa ttcggggaa gtacacggg 
      ggaggcggg aatgttggg atacaatga gatcgtcga 
      gttgcagaa agagggtgg atggaaggt tgtgtggga 
      cgacaccca gaaaaacac ccatatgta taaagggga 
      ccaatgggt tgcatttga cagtccgaa agcaatttc 
      taacaaagt cgagtatgc caagtcctt gaatctagg 
      aggtgtgat gatttggtc cattgaaac cgacgactt 
      tagaggcgt cagtggtac caagtaccc catcctcaa 
      ggccataca ccaaacact tggaggttc tagcaatga 
      aattgtgga acctgttcc ccaacccgt cgaagaagt 
      gacccagaa accatcctc gaccaaagc accagcacc 
      cccatctcg cgatttgac tacaactct ttgtactaa 
      agctggtta tgtccgaga tccagatga ttgttccat 
      cttttacta ttgtttggc ttacaatgg aggttttgt 
      tcctttgga acagagatg taacgccgg gttggtgtt 
      tgatgagga aaaattgtg gtgtgatta tcccgaagt 
      tgtaaagtg ttgaatgat tttggtgaa ataaattca 
      aaattgcag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY