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Gene information
General information
TC009540 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 2214897..2217637
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009540
    iBeetle screen iB_10262

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    antenna not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment sometimes unilateralnumber decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009540 RA
      MERVGLYLN KKIVVFLIG IVLSAVNAN LVHKKFEYK 
      YSFKPPYLA QKDGSVPFW EYGGNAIAS AEKVRIAPS 
      LRSQKGAIW TKNPINFDW WEVDIAFRV TGRGRVGAD 
      GLAFWYTQS KGAYDGEVF GSSDQWVGL GIFFDSFDN 
      DGKHNNPYI MAVVNDGTQ QFAHQLDGS TQMLAGCLR 
      DFRNKPFPT RAKIEYYQN ILTVLFHNG MTNNEQDYE 
      VCLRAENVV LPKSGYFGL SAATGGLAD DHDVIHFLT 
      NSLHLPGQI STSNAVPGE EQQKLTQEY QDYQRKLEQ 
      QKADYRKEH PDEQKDPDI DEWFESDSQ RELRQIFQG 
      QNQIFQVVR ELNNKLDEV VGRQERTMS LITQSAGGV 
      AAPSATGHA PAPAGGYAG DTIRRHEVD AVFNNQNQI 
      LNTAREIRQ FILELKTRA DTILNNQAK QPTAQVQSV 
      GYDTQSLIS EMRDGLNHV KQNVAQVAQ KIDQSPGGC 
      PSVSCVSVT TLLVITVVQ LLVILGYNI YRDNKDSQA 
      KKFY
    CDS sequence
    • >TC009540 RA
      atggagcga gtaggttta tatttaaat aaaaaaatt 
      gtagtgttt ttaattgga attgtatta agtgccgtg 
      aacgcaaat ctagtacat aaaaaattc gagtacaaa 
      tattcattc aagccccca tacttggcc caaaaggac 
      ggttcggta ccgttttgg gaatacggt ggaaatgcg 
      atagcgagt gcggaaaag gtgcgaatt gccccgtcc 
      cttcgcagc cagaagggg gctatatgg accaaaaac 
      cccatcaat ttcgactgg tgggaagtc gatattgcg 
      ttcagagtc acagggcgg gggcgcgtg ggggctgac 
      ggattggcg ttttggtac actcaaagc aagggggct 
      tatgatggc gaggttttc ggctcgtct gaccaatgg 
      gtgggtctt ggtatattt tttgactcg tttgacaat 
      gatggtaaa cacaacaac ccttacatc atggctgtg 
      gtcaacgat gggacccaa caattcgcc catcaactt 
      gatgggagt acgcaaatg ttggccggt tgtttgcgc 
      gatttccgt aacaaacct ttccccaca agggccaaa 
      attgaatat taccaaaat attctcaca gtattattc 
      cataatggt atgactaat aacgaacaa gattacgag 
      gtttgtttg agggctgaa aatgttgtt ttgcccaaa 
      agtggatat ttcggactt tcggcagcg acaggtgga 
      ttggctgat gatcatgac gtcattcat ttcttaacc 
      aatagctta catctaccg ggtcagatc agcacttcg 
      aatgcggtg ccgggggag gagcaacaa aagctgact 
      caggagtat caggattac caacggaaa ttagagcag 
      cagaaggcc gactatagg aaggagcat cctgatgag 
      cagaaagac cccgacatt gacgaatgg ttcgagtcg 
      gacagccag cgcgagttg cgccagatt ttccaaggc 
      cagaatcaa attttccaa gtcgtccgt gaattaaac 
      aacaaattg gacgaagtt gttggtcgt caagagcgc 
      acaatgagt ttaattaca caaagtgcc ggtggagtg 
      gcagcaccg tcggctaca ggccacgcc cctgcccca 
      gcaggtggt tacgcaggt gatacgatc aggagacac 
      gaagtcgat gctgttttt aataatcag aatcaaatt 
      ttgaacacg gcgagagaa attcggcaa tttatactg 
      gaattgaag acgagagct gacactatt ttgaataat 
      caagccaag caaccaacg gcccaagta cagtcagtg 
      gggtatgat acgcagtcg cttatttcc gaaatgaga 
      gatgggttg aaccatgtc aagcaaaat gtggctcaa 
      gtggcgcaa aaaatcgac caaagtcct ggcgggtgt 
      cctagtgtt tcgtgcgtt tcggtcact actttgttg 
      gttataaca gtggtgcag ttgttggtg attttaggg 
      tacaatatt tacagagat aataaagac agtcaagcg 
      aagaaattc tattag
    mRNA sequence
    • >TC009540 RA
      ttgctcaat tacaaattc aaactgtgc gcttgattt 
      aaacagggt gtttcgttc gttagattg gcaacgcca 
      ggccagacg attgtgcgc gtgttgtaa acatggagc 
      gagtaggtt tatatttaa ataaaaaaa ttgtagtgt 
      ttttaattg gaattgtat taagtgccg tgaacgcaa 
      atctagtac ataaaaaat tcgagtaca aatattcat 
      tcaagcccc catacttgg cccaaaagg acggttcgg 
      taccgtttt gggaatacg gtggaaatg cgatagcga 
      gtgcggaaa aggtgcgaa ttgccccgt cccttcgca 
      gccagaagg gggctatat ggaccaaaa accccatca 
      atttcgact ggtgggaag tcgatattg cgttcagag 
      tcacagggc gggggcgcg tgggggctg acggattgg 
      cgttttggt acactcaaa gcaaggggg cttatgatg 
      gcgaggttt tcggctcgt ctgaccaat gggtgggtc 
      ttggtatat tttttgact cgtttgaca atgatggta 
      aacacaaca acccttaca tcatggctg tggtcaacg 
      atgggaccc aacaattcg cccatcaac ttgatggga 
      gtacgcaaa tgttggccg gttgtttgc gcgatttcc 
      gtaacaaac ctttcccca caagggcca aaattgaat 
      attaccaaa atattctca cagtattat tccataatg 
      gtatgacta ataacgaac aagattacg aggtttgtt 
      tgagggctg aaaatgttg ttttgccca aaagtggat 
      atttcggac tttcggcag cgacaggtg gattggctg 
      atgatcatg acgtcattc atttcttaa ccaatagct 
      tacatctac cgggtcaga tcagcactt cgaatgcgg 
      tgccggggg aggagcaac aaaagctga ctcaggagt 
      atcaggatt accaacgga aattagagc agcagaagg 
      ccgactata ggaaggagc atcctgatg agcagaaag 
      accccgaca ttgacgaat ggttcgagt cggacagcc 
      agcgcgagt tgcgccaga ttttccaag gccagaatc 
      aaattttcc aagtcgtcc gtgaattaa acaacaaat 
      tggacgaag ttgttggtc gtcaagagc gcacaatga 
      gtttaatta cacaaagtg ccggtggag tggcagcac 
      cgtcggcta caggccacg cccctgccc cagcaggtg 
      gttacgcag gtgatacga tcaggagac acgaagtcg 
      atgctgttt ttaataatc agaatcaaa ttttgaaca 
      cggcgagag aaattcggc aatttatac tggaattga 
      agacgagag ctgacacta ttttgaata atcaagcca 
      agcaaccaa cggcccaag tacagtcag tggggtatg 
      atacgcagt cgcttattt ccgaaatga gagatgggt 
      tgaaccatg tcaagcaaa atgtggctc aagtggcgc 
      aaaaaatcg accaaagtc ctggcgggt gtcctagtg 
      tttcgtgcg tttcggtca ctactttgt tggttataa 
      cagtggtgc agttgttgg tgattttag ggtacaata 
      tttacagag ataataaag acagtcaag cgaagaaat 
      tctattaga tttggttgt gaaagttgt gagataagc 
      taagaatga cacccaatg gtcacaaaa attgttaac 
      cgtcttccc ttttattat ttattgtac agttacgtt 
      taatgattc ctaatgtgt ataataagt gatttttgt 
      ttgccactt actctgttt tgaaagact taaattcct 
      tttgtgtat atgtaagag tggaatgta cttttttac 
      atttttaga tgttaaatt aataaaagt cggtaaaga 
      ac
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