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Gene information
General information
TC009297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1783897..1788331
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009297
    iBeetle screen iB_10256

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009297 RA
      MATMMYPTL TKSQSMRSK RKSAVELLA ESKPFYVKS 
      EIVRDTTQA IPTRLPNPG PLGYGGYNR SKSVNVAPH 
      GTNYRPTTL PQYMAVSPS RSLPPLSHR RSVHLGSND 
      LLQNKLRQL LVCDSAEEL SVEQMAPLS PPAEYAQRC 
      HKSLPDLHC RAEPSSNKS SNKSLSNRD SGGSSGHYT 
      QRSEPAPPP RQFLQDSRR DSGSSTQHS GGSSYYCCQ 
      EPDCRARQA YPPPSTFKR QKCLRYKRD RPILRSKSD 
      ISDRYWRPP EQPPTSHLE QFFEHLGMT SDSYEEMLT 
      NSRSSESPV FFSDVSTVD SSRPLDNVD YCPSSFRTS 
      EPPSIVERN ARIIKWLCN CRKSQLT
    CDS sequence
    • >TC009297 RA
      atggccacc atgatgtac cccactttg accaagtcg 
      caaagcatg cgttcgaag cgcaagtct gcagtggag 
      ctcctcgcc gagagtaag cccttctac gtgaagtca 
      gaaattgtg agggacacc acacaggcc atccccacg 
      cggctgccc aacccgggg cccctggga tacgggggc 
      tacaacagg agcaagtcg gtcaacgtg gcgccccac 
      ggaaccaac tacaggccc accacactg ccccagtat 
      atggccgta tccccctcc cgctccctg ccacccctt 
      tcgcaccgc cgttcggtg catttgggc agcaacgac 
      cttctccaa aacaaatta cggcagttg ttagtttgt 
      gatagtgcg gaggaatta agtgtcgaa cagatggca 
      cctctgagt cctcccgcc gaatacgca caaaggtgt 
      cacaaaagc cttccagat cttcactgc agagctgaa 
      cctagttcg aataaaagt agcaacaag agtctttcc 
      aatagggac agtgggggc tccagtgga cattacacg 
      caacggagc gaaccggca ccgcccccg agacagttt 
      ttgcaggac agccgtcgc gactccggc tccagcacg 
      cagcatagc gggggcagc tcgtactac tgctgccag 
      gagcccgat tgccgcgcc cggcaagct tacccaccc 
      ccctcgact ttcaagcgt cagaaatgc cttcgatac 
      aaacgtgat cggccaatc cttcgctcg aaatcggac 
      atcagcgac cggtattgg cgccccccg gagcagccg 
      cccaccagc cacctggag cagttcttc gagcacctg 
      gggatgacc agcgacagc tacgaggag atgctgacc 
      aatagccgg tcgtcggaa agccccgtc ttcttctcg 
      gacgtctcc accgtggac tccagcaga ccgctcgac 
      aatgtcgat tactgcccc tcgtcgttc agaaccagc 
      gagccgcct tcgatagtc gaacgcaat gcccgcatc 
      atcaaatgg ctgtgtaac tgtcgaaaa tcgcagttg 
      acgtga
    mRNA sequence
    • >TC009297 RA
      tgcattttt gcagacaag acaacactc ctaaaccac 
      tctccagca gtttattgt aatttcaat tttcaaatt 
      cgaaaccct gcgcatgtg cgacacgca atcagctgg 
      agcagaaga aatggctcc tagcactcg gtcagtggt 
      gtaattcgc tcattaaca gcaaaaatt ccctcgaat 
      tatgtgata aaaaccact tggggcacc tccgacaca 
      gccctggcc aacgagttg tgagtagcg atgtgaagg 
      agtctccca gcccccacg atggccacc atgatgtac 
      cccactttg accaagtcg caaagcatg cgttcgaag 
      cgcaagtct gcagtggag ctcctcgcc gagagtaag 
      cccttctac gtgaagtca gaaattgtg agggacacc 
      acacaggcc atccccacg cggctgccc aacccgggg 
      cccctggga tacgggggc tacaacagg agcaagtcg 
      gtcaacgtg gcgccccac ggaaccaac tacaggccc 
      accacactg ccccagtat atggccgta tccccctcc 
      cgctccctg ccacccctt tcgcaccgc cgttcggtg 
      catttgggc agcaacgac cttctccaa aacaaatta 
      cggcagttg ttagtttgt gatagtgcg gaggaatta 
      agtgtcgaa cagatggca cctctgagt cctcccgcc 
      gaatacgca caaaggtgt cacaaaagc cttccagat 
      cttcactgc agagctgaa cctagttcg aataaaagt 
      agcaacaag agtctttcc aatagggac agtgggggc 
      tccagtgga cattacacg caacggagc gaaccggca 
      ccgcccccg agacagttt ttgcaggac agccgtcgc 
      gactccggc tccagcacg cagcatagc gggggcagc 
      tcgtactac tgctgccag gagcccgat tgccgcgcc 
      cggcaagct tacccaccc ccctcgact ttcaagcgt 
      cagaaatgc cttcgatac aaacgtgat cggccaatc 
      cttcgctcg aaatcggac atcagcgac cggtattgg 
      cgccccccg gagcagccg cccaccagc cacctggag 
      cagttcttc gagcacctg gggatgacc agcgacagc 
      tacgaggag atgctgacc aatagccgg tcgtcggaa 
      agccccgtc ttcttctcg gacgtctcc accgtggac 
      tccagcaga ccgctcgac aatgtcgat tactgcccc 
      tcgtcgttc agaaccagc gagccgcct tcgatagtc 
      gaacgcaat gcccgcatc atcaaatgg ctgtgtaac 
      tgtcgaaaa tcgcagttg acgtgaacg tacattcct 
      cacaatcac acaaatgac gcgcgcgaa tttatacat 
      tcatagcat tttaattct ttggggggg aagtaccgc 
      agtgtggca cgaacaata attcgaatc aatcaaagc 
      gacttgaca cgtaccaaa atttctgtt gtgataatt 
      ttttgttgt tttaggtag gagcgaaaa acgggaaag 
      aataaactg ctatgaggc gcaacggac agtttagct 
      cgaacaacg gtttgttga gtgaattaa atatttttt 
      attgttcca aacatactg tgcgtctag taaaaacta 
      tccttatcc atttttagt tgcaaattg ctcaaatga 
      aaccaaaac tgcctttgt aattgtcgt ggccgccta 
      ggtcatgcg atttgtagt gatagtcgt tatcagtat 
      tttaatcat tccctcctt tttatttcg atgtttcac 
      tcgacaaag attcagaca ataagagct aaactgtgc 
      gttggcatt cccacaatg tcactgttt tgactcgtt 
      gtgtgttct aatgtaatt agatgacta aactttttt 
      actttgaga agtagaacg aatttctac gtggatcaa 
      tatattact cgattaaaa gtgataaca atatgctta 
      ttaagatac gaatgtttc ttcaaagct attatcgaa 
      ttcgtgtat tgtgaataa acataatca gtgatgaat 
      tagcaatgt tgaatattt aaatgtaat catgtttaa 
      ttgttttcc ttacattcg cacgttgag gtgtctgat 
      ctgtttctc tctcacagt attttttcg gcttgccta 
      atcgtttct taaataatt tagcaataa tcccgaatt 
      aatcatagg ttttatcga gctaacagc tcctgttaa 
      tcagttaat tgaaataat atgtttaac gtttttata 
      gtttgggtc aaattaaca cgaacgtgt taattaatc 
      gcgaataca tgtgtacat acatagtta tagcttcta 
      gttaagtac ccatgtaat ggactgctg ttagttacg 
      gtgctgtgc atcgattct gtctagtct gtacttaaa 
      ttctcattg tttcaatat agagcatag ctatgttta 
      cttaattct tacaaaata tattattct gcaatat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY