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Gene information
General information
TC009324 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 396365..409601
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009324
    iBeetle screen iB_10252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009324 RA
      MANTSNYKL SAAVSNLKY DDILPYKTQ NGTVTNEFL 
      QKLIEILLD FVRATNDRN EKILDFHHP AEMQALLDL 
      AIPNKGVSL QQLIHDCAT TLKYQVKTG HPRFFNQLS 
      CGLDIISMA GEWLTATAN TNMFTYEIA PVFILMENV 
      VMTHMREII GSNWVNGDS ILAPGGSIS NLYAFLAAR 
      HKMFPNYKE KGIATIQGQ LVMFTSDQS HYSVKSCAS 
      ICGLGTDNC LMVPSDIHG RMIPSELER LILDRKAKG 
      HIPFFVCCT SGTTVLGAF DPINEIADI CEKYGMWLH 
      VDAAWGGGL LLSKKYRHP RLSGVERAD SVTWNPHKL 
      MGALLQCST IHFKEDGLL MSCNQMSAD YLFMQDKLY 
      DIKYDTGDK VIQCGRHND IFKLWLQWR AKGDDGFEK 
      HMDRLMELS EYMVKRIKE QPDKFYLIM EPELVNVSF 
      WYIPTRLRK MPHSPAREQ ELGKMCPIL KARMMQTGT 
      LMVGYQPDD RRPNFFRNI ISSAAVTES DVDFLLSEM 
      DRLGHDL
    CDS sequence
    • >TC009324 RA
      atggccaac acttccaac tataagctc agtgcggct 
      gtttcaaac ctcaaatat gatgacatt ctcccttac 
      aagacccaa aacggcacc gtaaccaac gaattcctg 
      caaaagctg atcgaaatt cttctggat tttgtacga 
      gcgaccaac gaccgcaat gaaaaaatc ctggatttc 
      caccatcca gcagaaatg caagcacta ctggacctc 
      gcgattcct aataaaggg gtatcacta cagcagctc 
      atccacgac tgcgctact actctcaag tatcaggtc 
      aaaacaggt catccacga tttttcaac cagctttct 
      tgtggactc gacataatc tcgatggca ggcgaatgg 
      ctaacagcc acagctaac accaacatg ttcacttac 
      gaaatcgct cctgtcttc atcctgatg gaaaacgtc 
      gtgatgaca catatgaga gagatcatc ggaagtaat 
      tgggtcaat ggtgactca atcctagct ccaggtggt 
      tcaatatcg aacttgtac gcgtttttg gcggcacgt 
      cacaagatg tttccgaat tataaggaa aaaggcata 
      gccacgata caaggacaa ctagttatg ttcacctct 
      gatcaaagc cactattcc gtgaaatcc tgcgcttca 
      atttgcggc ttaggtacc gacaattgt ttaatggtg 
      ccttcggac attcacggt cgtatgatt ccatcagaa 
      ttggaacgt ctgatcctt gataggaaa gccaagggt 
      cacattccc ttcttcgtt tgttgcact tccggaact 
      acagtcttg ggagctttc gatccgatc aacgaaatt 
      gccgatatt tgcgaaaaa tacggcatg tggctgcat 
      gttgatgcc gcttggggt ggaggtctg ctcctgtcc 
      aagaaatac cgacatcca aggctaagt ggagtcgaa 
      cgggccgat tcagtgaca tggaacccc cacaaactg 
      atgggcgca cttttacag tgttccacc atccatttc 
      aaagaggac ggattattg atgagttgt aaccagatg 
      tctgcggat tatctcttc atgcaggat aaactgtac 
      gatatcaag tacgacacc ggcgataaa gtcattcag 
      tgtggccgt cataacgac atcttcaaa ttgtggctg 
      cagtggaga gctaagggc gacgatggc tttgaaaaa 
      cacatggac cgcttgatg gaactgtcc gaatacatg 
      gtaaaacgt attaaagaa caacccgat aagttttac 
      ttgatcatg gaaccggaa ctagtcaat gttagtttt 
      tggtacatt ccgacccgt ctccgcaaa atgccgcat 
      tcgccggcc cgtgaacaa gagctcgga aaaatgtgt 
      cccattttg aaggctcga atgatgcaa actggaact 
      ctcatggtt gggtaccag ccagatgac agaaggccc 
      aactttttc cgaaatatt atttcttcg gctgctgtg 
      accgaaagt gacgttgat ttcctcctg tctgaaatg 
      gaccgactt gggcacgac ttgtaa
    mRNA sequence
    • >TC009324 RA
      tggctccac cccaggaga cgtgcctcc tacacgctg 
      acgtcaaaa ttgcgcgcg ccagcttcg aaatgggtc 
      acctagtgt gacctttgg aacgggctt tcggacgag 
      cttttagac gccgtacag cttccagaa gctccagcc 
      ttcgtgatg gccaacact tccaactat aagctcagt 
      gcggctgtt tcaaacctc aaatatgat gacattctc 
      ccttacaag acccaaaac ggcaccgta accaacgaa 
      ttcctgcaa aagctgatc gaaattctt ctggatttt 
      gtacgagcg accaacgac cgcaatgaa aaaatcctg 
      gatttccac catccagca gaaatgcaa gcactactg 
      gacctcgcg attcctaat aaaggggta tcactacag 
      cagctcatc cacgactgc gctactact ctcaagtat 
      caggtcaaa acaggtcat ccacgattt ttcaaccag 
      ctttcttgt ggactcgac ataatctcg atggcaggc 
      gaatggcta acagccaca gctaacacc aacatgttc 
      acttacgaa atcgctcct gtcttcatc ctgatggaa 
      aacgtcgtg atgacacat atgagagag atcatcgga 
      agtaattgg gtcaatggt gactcaatc ctagctcca 
      ggtggttca atatcgaac ttgtacgcg tttttggcg 
      gcacgtcac aagatgttt ccgaattat aaggaaaaa 
      ggcatagcc acgatacaa ggacaacta gttatgttc 
      acctctgat caaagccac tattccgtg aaatcctgc 
      gcttcaatt tgcggctta ggtaccgac aattgttta 
      atggtgcct tcggacatt cacggtcgt atgattcca 
      tcagaattg gaacgtctg atccttgat aggaaagcc 
      aagggtcac attcccttc ttcgtttgt tgcacttcc 
      ggaactaca gtcttggga gctttcgat ccgatcaac 
      gaaattgcc gatatttgc gaaaaatac ggcatgtgg 
      ctgcatgtt gatgccgct tggggtgga ggtctgctc 
      ctgtccaag aaataccga catccaagg ctaagtgga 
      gtcgaacgg gccgattca gtgacatgg aacccccac 
      aaactgatg ggcgcactt ttacagtgt tccaccatc 
      catttcaaa gaggacgga ttattgatg agttgtaac 
      cagatgtct gcggattat ctcttcatg caggataaa 
      ctgtacgat atcaagtac gacaccggc gataaagtc 
      attcagtgt ggccgtcat aacgacatc ttcaaattg 
      tggctgcag tggagagct aagggcgac gatggcttt 
      gaaaaacac atggaccgc ttgatggaa ctgtccgaa 
      tacatggta aaacgtatt aaagaacaa cccgataag 
      ttttacttg atcatggaa ccggaacta gtcaatgtt 
      agtttttgg tacattccg acccgtctc cgcaaaatg 
      ccgcattcg ccggcccgt gaacaagag ctcggaaaa 
      atgtgtccc attttgaag gctcgaatg atgcaaact 
      ggaactctc atggttggg taccagcca gatgacaga 
      aggcccaac tttttccga aatattatt tcttcggct 
      gctgtgacc gaaagtgac gttgatttc ctcctgtct 
      gaaatggac cgacttggg cacgacttg taaactact 
      caattgttg aattgttgt tttcgtttg ttgtactta 
      tttattcgt gttattcct atctactca gcctgtttc 
      tgctaaaat tttctctca aattatgat attagttcg 
      attaggtgt gtagtagag aaatataca aacatatca 
      tcgcgtttt taatttttt cggtccaga aatggccga 
      aataattca tttgttcct tatcttatc ataaaacta 
      actcgtatt gttaatagc acactttat ttgtgttaa 
      ttgaatgac aaaaccttg aaaggtcaa ttaccaatt 
      tacgttaaa ttgaaagtc aattaaatc gctcgttta 
      ttaataggt aaacttagg aatagaaaa ttaggtgcc 
      aaaaatata gggagtttc gtttcaaat tgtccactc 
      tggacaaac ttaaccgaa acctgcttt ataacaaaa 
      ttagataat ttggacgca gacgagtta atttataaa 
      aagtaaagt caaccaatt ttatcaact aaaaacata 
      tcagctaga gtagtttcg taaaaataa gttgagaga 
      aaatttttc gacgtaaaa aatctcgat gtgttaaca 
      tttgtgata gaagttgat ttaatcgat gtccagatt 
      ttatcatta cttataatt tattaaacc attgttaaa 
      taatgtctt aaatgtaaa atattgcat tagaaaata 
      ccaaaaaac actaagtgt gcgtagcgt atatttcta 
      atatggtaa aaaatgtgt agctgacgc aataatgtt 
      gtatagaaa agtttcgag tttagttcg ctaaattag 
      aactaagtc aaatggtgc tttttctgt tttgttcca 
      tttattttc ttcactaaa cactagaaa gagtcctac 
      aaaattagg ggaagcacc agttatatt ttacggtcc 
      aaagaaaac atatataac gagttcaag tgagttgtt 
      atagatgtt tatatggtt agaaatgaa atgtataaa 
      attgttgaa actgccaaa ttatagcat attcgttgt 
      ttattgagt tgttatcca attcagcgg tctttatcg 
      atttgttac ttcctgtgt ggaacaaca aaacacaaa 
      gtcttccat tttatatga aatattatt tgaaaaaaa 
      aaatcgctt gtttgtggc gacatgtac atgtaccga 
      tatgttatt atacagagt ggcccaaaa ttcttaaca 
      ttttttgcg aattttggg tccaaagta ttatattgc 
      ctaaatgta aaaaatcaa agtatagag taacctcgt 
      ttattagtt gcttcttag catccaaga attattgta 
      aagatcctg tatgtaaag tatttatcg aataaaatg 
      tatatatat ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY