Status
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Gene information
General information
TC033536
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009338,TC009339
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 554775..579129
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033536
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10250

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    yolk late sometimesdecreased - Number of animals showing the phenotype: 3

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva potentially circumference decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033536 RA
      MVMSEQGGL AMHQLPMHH RGLVQPSLV MNPHPINDS 
      CSMDSQNKK PRRLSHCVG CGGQIHDQY ILRVAPDLE 
      WHAACLKCQ ECRQFLDES CTCFVRDGK TYCKRDYVR 
      LFGAKCDRC GSSFSKDDF VMRAKSKIY HIKCFKCCA 
      CSRPLVPGD EFALRDGGN LYCKEDHDH MEKTSIPTT 
      PHLPSTESN NNTSLSNNN HNSSSTELG CMSDSGSES 
      GSHKGGGGG LRKGSGGGG GGSDGKPTR VRTVLNEKQ 
      LHTLRTCYA ANPRPDALM KEQLVEMTG LSPRVIRVW 
      FQNKRCKDK KKTLLMKQQ MQQEKDGRK LGYGAMQGI 
      PMVASSPVR HDSPIGVHP LEVQAYQPP WKALSDFAL 
      HADLEQPHQ PAFQQLVNQ MHGYDLPPP SMGPATGPP 
      PGMLPSSAP PSDVMTHPD STDSYVTYL ESDESLPAS 
      P
    • >TC033536 RB
      MVMSEQGGL AMHQLPMHH RGLVQPSLV MNPHPINDS 
      CSMDSQNKK PRRLSHCVG CGGQIHDQY ILRVAPDLE 
      WHAACLKCQ ECRQFLDES CTCFVRDGK TYCKRDYVR 
      LFGAKCDRC GSSFSKDDF VMRAKSKIY HIKCFKCCA 
      CSRPLVPGD EFALRDGGN LYCKEDHDH MEKTSIPTT 
      PHLPSTESN NNTSLSNNN HNSSSTELG CMSDSGSES 
      GSHKGGGGG LRKGSGGGG GGSDGKPTR VRTVLNEKQ 
      LHTLRTCYA ANPRPDALM KEQLVEMTG LSPRVIRVW 
      FQNKRCKDK KKTLLMKQQ MQQEKYYVQ DGRKLGYGA 
      MQGIPMVAS SPVRHDSPI GVHPLEVQA YQPPWKALS 
      DFALHADLE QPHQPAFQQ LVNQMHGYD LPPPSMGPA 
      TGPPPGMLP SSAPPSDVM THPDSTDSY VTYLESDES 
      LPASP
    CDS sequence
    • >TC033536 RA
      atggtgatg tcagagcaa ggcggactg gccatgcac 
      cagttaccg atgcatcac aggggtttg gtgcagccc 
      agccttgtc atgaacccg catcccatc aacgattcc 
      tgctccatg gactcgcag aacaaaaag ccccgccgg 
      ctgagccac tgcgtgggg tgcggtggc cagatccac 
      gaccaatac atcctccgg gtagctcct gacctggaa 
      tggcacgca gcttgtcta aaatgccag gagtgtcga 
      cagttcctt gacgaatcc tgcacatgc ttcgtgcga 
      gatggtaaa acctactgc aagcgagac tatgtgagg 
      ttgtttggg gcgaagtgc gacaggtgc gggtcgtcg 
      tttagcaaa gatgatttt gtgatgcgg gcaaaaagc 
      aagatttat cacataaag tgcttcaag tgttgtgct 
      tgttcacga ccgttagta cctggagat gagttcgcg 
      ttaagggac ggtggaaat ttgtactgt aaggaggac 
      cacgaccac atggagaag acgtccata ccaacaacg 
      ccacatttg ccctccacc gaaagtaat aacaacacc 
      agcttgagc aacaacaat cacaacagc agttccacg 
      gagctgggc tgcatgtca gattccggc tcggagagc 
      gggtcgcac aagggcggc ggcggcggc ttgaggaag 
      ggctccggg ggcggcggc ggcggcagc gacggcaag 
      ccgacgcgc gtgcgcacc gtcctcaac gagaaacaa 
      ttgcacacg ttgaggacg tgctatgcc gccaatcct 
      aggcctgat gccctcatg aaggagcag ctggtggaa 
      atgacggga ctttccccg agagtcatc agggtttgg 
      ttccagaac aagaggtgc aaggacaaa aaaaagacc 
      ctgcttatg aagcagcag atgcaacag gaaaaggat 
      gggcgaaaa ttgggttac ggtgccatg cagggtatc 
      ccaatggta gcgtcatca cctgtacga cacgattca 
      cccatcggt gtccatccc cttgaagta caagcttac 
      caaccacca tggaaagcc ctatcagat ttcgccctt 
      catgcagac ttagagcaa ccccatcag ccggccttc 
      cagcagctc gtcaaccag atgcatggg tacgacctg 
      ccgcccccg tcgatgggc cccgccacc ggcccaccc 
      ccgggtatg ctgccctca tcagcgccg ccgtctgac 
      gtcatgacg catcccgac agcaccgat agttacgtt 
      acctacctc gaaagcgac gagagcctt cccgccagt 
      ccctaa
    • >TC033536 RB
      atggtgatg tcagagcaa ggcggactg gccatgcac 
      cagttaccg atgcatcac aggggtttg gtgcagccc 
      agccttgtc atgaacccg catcccatc aacgattcc 
      tgctccatg gactcgcag aacaaaaag ccccgccgg 
      ctgagccac tgcgtgggg tgcggtggc cagatccac 
      gaccaatac atcctccgg gtagctcct gacctggaa 
      tggcacgca gcttgtcta aaatgccag gagtgtcga 
      cagttcctt gacgaatcc tgcacatgc ttcgtgcga 
      gatggtaaa acctactgc aagcgagac tatgtgagg 
      ttgtttggg gcgaagtgc gacaggtgc gggtcgtcg 
      tttagcaaa gatgatttt gtgatgcgg gcaaaaagc 
      aagatttat cacataaag tgcttcaag tgttgtgct 
      tgttcacga ccgttagta cctggagat gagttcgcg 
      ttaagggac ggtggaaat ttgtactgt aaggaggac 
      cacgaccac atggagaag acgtccata ccaacaacg 
      ccacatttg ccctccacc gaaagtaat aacaacacc 
      agcttgagc aacaacaat cacaacagc agttccacg 
      gagctgggc tgcatgtca gattccggc tcggagagc 
      gggtcgcac aagggcggc ggcggcggc ttgaggaag 
      ggctccggg ggcggcggc ggcggcagc gacggcaag 
      ccgacgcgc gtgcgcacc gtcctcaac gagaaacaa 
      ttgcacacg ttgaggacg tgctatgcc gccaatcct 
      aggcctgat gccctcatg aaggagcag ctggtggaa 
      atgacggga ctttccccg agagtcatc agggtttgg 
      ttccagaac aagaggtgc aaggacaaa aaaaagacc 
      ctgcttatg aagcagcag atgcaacag gaaaagtat 
      tacgtgcag gatgggcga aaattgggt tacggtgcc 
      atgcagggt atcccaatg gtagcgtca tcacctgta 
      cgacacgat tcacccatc ggtgtccat ccccttgaa 
      gtacaagct taccaacca ccatggaaa gccctatca 
      gatttcgcc cttcatgca gacttagag caaccccat 
      cagccggcc ttccagcag ctcgtcaac cagatgcat 
      gggtacgac ctgccgccc ccgtcgatg ggccccgcc 
      accggccca cccccgggt atgctgccc tcatcagcg 
      ccgccgtct gacgtcatg acgcatccc gacagcacc 
      gatagttac gttacctac ctcgaaagc gacgagagc 
      cttcccgcc agtccctaa
    mRNA sequence
    • >TC033536 RA
      cggttgaac catcatcag ttgatccgg aactgttgc 
      tggaagtgg ttgtgtgtg cgaatgaat aaggtttag 
      tacttggac ttaaaaaat aagttcgtt cgtcggctg 
      tgccgccgt gtgcttctg gtggactgt tctttgacg 
      tgcggttcg tatggtgat gtcagagca aggcggact 
      ggccatgca ccagttacc gatgcatca caggggttt 
      ggtgcagcc cagccttgt catgaaccc gcatcccat 
      caacgattc ctgctccat ggactcgca gaacaaaaa 
      gccccgccg gctgagcca ctgcgtggg gtgcggtgg 
      ccagatcca cgaccaata catcctccg ggtagctcc 
      tgacctgga atggcacgc agcttgtct aaaatgcca 
      ggagtgtcg acagttcct tgacgaatc ctgcacatg 
      cttcgtgcg agatggtaa aacctactg caagcgaga 
      ctatgtgag gttgtttgg ggcgaagtg cgacaggtg 
      cgggtcgtc gtttagcaa agatgattt tgtgatgcg 
      ggcaaaaag caagattta tcacataaa gtgcttcaa 
      gtgttgtgc ttgttcacg accgttagt acctggaga 
      tgagttcgc gttaaggga cggtggaaa tttgtactg 
      taaggagga ccacgacca catggagaa gacgtccat 
      accaacaac gccacattt gccctccac cgaaagtaa 
      taacaacac cagcttgag caacaacaa tcacaacag 
      cagttccac ggagctggg ctgcatgtc agattccgg 
      ctcggagag cgggtcgca caagggcgg cggcggcgg 
      cttgaggaa gggctccgg gggcggcgg cggcggcag 
      cgacggcaa gccgacgcg cgtgcgcac cgtcctcaa 
      cgagaaaca attgcacac gttgaggac gtgctatgc 
      cgccaatcc taggcctga tgccctcat gaaggagca 
      gctggtgga aatgacggg actttcccc gagagtcat 
      cagggtttg gttccagaa caagaggtg caaggacaa 
      aaaaaagac cctgcttat gaagcagca gatgcaaca 
      ggaaaagga tgggcgaaa attgggtta cggtgccat 
      gcagggtat cccaatggt agcgtcatc acctgtacg 
      acacgattc acccatcgg tgtccatcc ccttgaagt 
      acaagctta ccaaccacc atggaaagc cctatcaga 
      tttcgccct tcatgcaga cttagagca accccatca 
      gccggcctt ccagcagct cgtcaacca gatgcatgg 
      gtacgacct gccgccccc gtcgatggg ccccgccac 
      cggcccacc cccgggtat gctgccctc atcagcgcc 
      gccgtctga cgtcatgac gcatcccga cagcaccga 
      tagttacgt tacctacct cgaaagcga cgagagcct 
      tcccgccag tccctaaca cccccacaa ctagccgtc 
      gccgcttcc gcccctaaa cgctctcac accccttct 
      cacctcttt cctccttta ccaccacca aggactgat 
      tcggcggct gtgcagacc accacccta gtaaccacc 
      accatcatg gctacaatt tcaggacga taaaggaac 
      gaacaaaac gaacaatca cttttttgg tttttcatc 
      atcacaata agtggccag aacgaatct caatttaat 
      caaaatcaa aatttcaac acatatcgc cactttgcg 
      ttgatctca ctcgagagt catcgaagt tttcacatg 
      gtttttgtt ggccagtct caatattcc ttgttttac 
      gtacagttg gagaaaaaa tactgaatg gtatttttt 
      tccggcttt acagttgta tcattagat gttttgttg 
      atgttgtta ttgtcgggg tatgcgtga agcagtttt 
      ttactaata cactgtacc atattgtaa aattatata 
      tctaatgta acatttgat tgtacaatt tgttatgga 
      aaatctaac tttgtcgct aatttgtag gtgttatta 
      attttagat ttactactg tgcattcga atgtatgat 
      atttttaca gagctctgt atcacataa tttattgca 
      atgaattta taaaactgt atttgataa gaaataaaa 
      cagcaagtt atctgtaaa taatgttac attaaaaaa 
      ttaatttgt aaataaata catgagata atc
    • >TC033536 RB
      cggttgaac catcatcag ttgatccgg aactgttgc 
      tggaagtgg ttgtgtgtg cgaatgaat aaggtttag 
      tacttggac ttaaaaaat aagttcgtt cgtcggctg 
      tgccgccgt gtgcttctg gtggactgt tctttgacg 
      tgcggttcg tatggtgat gtcagagca aggcggact 
      ggccatgca ccagttacc gatgcatca caggggttt 
      ggtgcagcc cagccttgt catgaaccc gcatcccat 
      caacgattc ctgctccat ggactcgca gaacaaaaa 
      gccccgccg gctgagcca ctgcgtggg gtgcggtgg 
      ccagatcca cgaccaata catcctccg ggtagctcc 
      tgacctgga atggcacgc agcttgtct aaaatgcca 
      ggagtgtcg acagttcct tgacgaatc ctgcacatg 
      cttcgtgcg agatggtaa aacctactg caagcgaga 
      ctatgtgag gttgtttgg ggcgaagtg cgacaggtg 
      cgggtcgtc gtttagcaa agatgattt tgtgatgcg 
      ggcaaaaag caagattta tcacataaa gtgcttcaa 
      gtgttgtgc ttgttcacg accgttagt acctggaga 
      tgagttcgc gttaaggga cggtggaaa tttgtactg 
      taaggagga ccacgacca catggagaa gacgtccat 
      accaacaac gccacattt gccctccac cgaaagtaa 
      taacaacac cagcttgag caacaacaa tcacaacag 
      cagttccac ggagctggg ctgcatgtc agattccgg 
      ctcggagag cgggtcgca caagggcgg cggcggcgg 
      cttgaggaa gggctccgg gggcggcgg cggcggcag 
      cgacggcaa gccgacgcg cgtgcgcac cgtcctcaa 
      cgagaaaca attgcacac gttgaggac gtgctatgc 
      cgccaatcc taggcctga tgccctcat gaaggagca 
      gctggtgga aatgacggg actttcccc gagagtcat 
      cagggtttg gttccagaa caagaggtg caaggacaa 
      aaaaaagac cctgcttat gaagcagca gatgcaaca 
      ggaaaagta ttacgtgca ggatgggcg aaaattggg 
      ttacggtgc catgcaggg tatcccaat ggtagcgtc 
      atcacctgt acgacacga ttcacccat cggtgtcca 
      tccccttga agtacaagc ttaccaacc accatggaa 
      agccctatc agatttcgc ccttcatgc agacttaga 
      gcaacccca tcagccggc cttccagca gctcgtcaa 
      ccagatgca tgggtacga cctgccgcc cccgtcgat 
      gggccccgc caccggccc acccccggg tatgctgcc 
      ctcatcagc gccgccgtc tgacgtcat gacgcatcc 
      cgacagcac cgatagtta cgttaccta cctcgaaag 
      cgacgagag ccttcccgc cagtcccta acaccccca 
      caactagcc gtcgccgct tccgcccct aaacgctct 
      cacacccct tctcacctc tttcctcct ttaccacca 
      ccaaggact gattcggcg gctgtgcag accaccacc 
      ctagtaacc accaccatc atggctaca atttcagga 
      cgataaagg aacgaacaa aacgaacaa tcacttttt 
      tggtttttc atcatcaca ataagtggc cagaacgaa 
      tctcaattt aatcaaaat caaaatttc aacacatat 
      cgccacttt gcgttgatc tcactcgag agtcatcga 
      agttttcac atggttttt gttggccag tctcaatat 
      tccttgttt tacgtacag ttggagaaa aaatactga 
      atggtattt ttttccggc tttacagtt gtatcatta 
      gatgttttg ttgatgttg ttattgtcg gggtatgcg 
      tgaagcagt tttttacta atacactgt accatattg 
      taaaattat atatctaat gtaacattt gattgtaca 
      atttgttat ggaaaatct aactttgtc gctaatttg 
      taggtgtta ttaatttta gatttacta ctgtgcatt 
      cgaatgtat gatattttt acagagctc tgtatcaca 
      taatttatt gcaatgaat ttataaaac tgtatttga 
      taagaaata aaacagcaa gttatctgt aaataatgt 
      tacattaaa aaattaatt tgtaaataa atacatgag 
      ataatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY