Status
Please wait ...
Gene information
General information
TC030715 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 637465..642623
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030715
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10249

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030715 RA
      MCEESACLV PPLEQNQNG PNESRLSSG STAGTSHNG 
      EALVGFHED ALSQAAVGK AQLLLALVL GLGLAADCS 
      ELTILGYIL PAAELQLCI DEHKKGWLV SITLLALAG 
      GSLAWGILG DHLGRRRAL ISALSVAAL FSAVATVMP 
      TYGTFMTAR FCSGLGVAG AFPLTFSYL SETCSRATR 
      TRYTGLLYS FWPLGAIFT SLIAHLTMP TSGAEIVED 
      NREHWSSWH RFLILSILP TVGCIVGLI WASESPRYL 
      LEASREVEA LAVYQRLHR LNKARTQYG LTELELPGR 
      SAYRERPMS PSRNLFGHG LESFREAFQ RISSPTHFR 
      TTLLLAALH LIVGFTYMG ISNFSTSMI KQIKDQEYF 
      SERNYIFDK NITGVIFNT TLENVEYKN TLFKNVTFK 
      HINLNHVEF LNCTIDEAE FINVKSSIT YFRNSTIKD 
      SRFVDTDLT HHHFLNCTL SNNTFMSLI SDCIVDFDY 
      NIYLDDLYE DTIAWAGSM FPALLFMGF ILETASRPP 
      IIFTTMGTA SITCIGIFF WTNTFVVTL IELTVKIML 
      MCAVNAVNM VVVEGYPCH LRCTAHGMM RSLFHLASL 
      CAIPIYGMF GHTMLMFPA VITISLTFG AALLSLKIQ 
      DNSKVLL
    CDS sequence
    • >TC030715 RA
      atgtgtgag gaatccgca tgcttggta cctccgttg 
      gaacagaat caaaatggg ccaaacgaa tcccgactg 
      tcatcagga agcaccgca ggcacaagc cacaacggt 
      gaagccctg gttggcttc catgaagac gccttatct 
      caagcagct gtgggcaaa gcccagctc ctcctagcc 
      ctagtttta gggcttggc cttgctgcc gactgctcg 
      gaacttaca attctgggc tacatactc ccagctgct 
      gaactccaa ctgtgtata gacgaacac aagaaggga 
      tggcttgtt tccatcacg cttttagcc cttgctgga 
      ggttccctt gcgtgggga atcctggga gatcattta 
      ggccgtcga agggcccta atcagtgcc ctttctgtt 
      gctgctctt ttctcagcg gtcgcaaca gtgatgcca 
      acgtatgga accttcatg acagcgagg ttttgttcg 
      ggattaggg gtcgcagga gcgtttcct ttaactttt 
      agttatttg tctgaaact tgttcgcgt gctacgagg 
      acgaggtac acaggactg ttgtattcg ttttggcct 
      ttgggggct atttttacg tctttgatt gcccatcta 
      actatgccc actagtggg gctgagata gttgaggat 
      aatagggaa cattggagt tcgtggcac cgttttctc 
      attttgagt attcttccg actgttggc tgtattgtt 
      gggttgatt tgggcgtct gagtcgcca aggtacctt 
      ctggaagct tcacgagaa gtcgaagct ttggctgtg 
      taccagaga cttcacagg ttgaataaa gcaaggact 
      cagtatggg ttaactgaa ctggaattg ccaggaagg 
      agtgcatac agggaaaga cccatgagt ccatcgaga 
      aatcttttt gggcatggt ttggagtct tttcgagaa 
      gctttccaa cgcatctct tctccaaca cacttccgc 
      acaactctg cttctggca gccttgcac ctcatcgta 
      ggtttcacc tacatggga atatcaaac ttttcgacg 
      tccatgatt aagcaaatt aaagatcaa gagtatttt 
      tcggagcga aattatata tttgataag aatatcacc 
      ggagtgatt ttcaacaca actttagaa aatgttgaa 
      tacaaaaac actcttttt aaaaatgtg actttcaaa 
      catatcaac ttgaaccac gtagaattc ttgaactgt 
      actattgac gaagccgaa tttattaac gtaaagtcg 
      tccatcacc tacttcaga aactcgacc attaaggat 
      tcgaggttt gtggacact gacctcacc catcaccat 
      ttcctcaac tgtacgttg agtaacaac accttcatg 
      tctttgata tctgattgt atcgtcgat tttgattat 
      aatatctat ttggatgat ttgtacgag gacacaata 
      gcatgggct ggttccatg ttcccggct ttacttttc 
      atgggtttt attctggag actgcatca agaccaccc 
      atcattttt accaccatg gggaccgct agcatcacc 
      tgcattggc attttcttc tggacgaat actttcgtt 
      gtgacactt attgaacta acagtcaaa attatgctg 
      atgtgtgcc gtaaatgcc gttaacatg gtggtagtc 
      gaaggatac ccttgtcat ttaagatgt accgcccat 
      ggtatgatg cgcagttta ttccactta gcgtccctt 
      tgtgccatc cccatctat ggtatgttt ggtcacaca 
      atgttaatg ttccccgct gtgatcacc atttctttg 
      acttttggg gccgctttg ttgtctttg aagatacaa 
      gacaacagt aaagttctc ttgtaa
    mRNA sequence
    • >TC030715 RA
      agcgagtcg tgtttttcc gtatgacct ccctttgct 
      taaagttgc ttttttcag gttgaaact aaaggttac 
      gtaggtaat aaaagcgcc cctttgctg ttgcgcaga 
      agggccgtt attgtaata aaatgcagc aaagggtgc 
      gtgtggtcg attcgatct ggacgtgtc gtttaaaca 
      atcaacaaa ggtacagtg taaagggtg cattacgtg 
      agcttttgc attattgat cgcgggaag agaggcttg 
      aatcgatat tgtgtccag tagcgtgac actcgtagc 
      ttttgcaat aacaactat cctttatac tggtttact 
      gaacctacc acagatggt tgatgagcg tggtaatga 
      cgaaagctt aaaccggcg cacaacagg gggtaagga 
      tgtgtgagg aatccgcat gcttggtac ctccgttgg 
      aacagaatc aaaatgggc caaacgaat cccgactgt 
      catcaggaa gcaccgcag gcacaagcc acaacggtg 
      aagccctgg ttggcttcc atgaagacg ccttatctc 
      aagcagctg tgggcaaag cccagctcc tcctagccc 
      tagttttag ggcttggcc ttgctgccg actgctcgg 
      aacttacaa ttctgggct acatactcc cagctgctg 
      aactccaac tgtgtatag acgaacaca agaagggat 
      ggcttgttt ccatcacgc ttttagccc ttgctggag 
      gttcccttg cgtggggaa tcctgggag atcatttag 
      gccgtcgaa gggccctaa tcagtgccc tttctgttg 
      ctgctcttt tctcagcgg tcgcaacag tgatgccaa 
      cgtatggaa ccttcatga cagcgaggt tttgttcgg 
      gattagggg tcgcaggag cgtttcctt taactttta 
      gttatttgt ctgaaactt gttcgcgtg ctacgagga 
      cgaggtaca caggactgt tgtattcgt tttggcctt 
      tgggggcta tttttacgt ctttgattg cccatctaa 
      ctatgccca ctagtgggg ctgagatag ttgaggata 
      atagggaac attggagtt cgtggcacc gttttctca 
      ttttgagta ttcttccga ctgttggct gtattgttg 
      ggttgattt gggcgtctg agtcgccaa ggtaccttc 
      tggaagctt cacgagaag tcgaagctt tggctgtgt 
      accagagac ttcacaggt tgaataaag caaggactc 
      agtatgggt taactgaac tggaattgc caggaagga 
      gtgcataca gggaaagac ccatgagtc catcgagaa 
      atctttttg ggcatggtt tggagtctt ttcgagaag 
      ctttccaac gcatctctt ctccaacac acttccgca 
      caactctgc ttctggcag ccttgcacc tcatcgtag 
      gtttcacct acatgggaa tatcaaact tttcgacgt 
      ccatgatta agcaaatta aagatcaag agtattttt 
      cggagcgaa attatatat ttgataaga atatcaccg 
      gagtgattt tcaacacaa ctttagaaa atgttgaat 
      acaaaaaca ctcttttta aaaatgtga ctttcaaac 
      atatcaact tgaaccacg tagaattct tgaactgta 
      ctattgacg aagccgaat ttattaacg taaagtcgt 
      ccatcacct acttcagaa actcgacca ttaaggatt 
      cgaggtttg tggacactg acctcaccc atcaccatt 
      tcctcaact gtacgttga gtaacaaca ccttcatgt 
      ctttgatat ctgattgta tcgtcgatt ttgattata 
      atatctatt tggatgatt tgtacgagg acacaatag 
      catgggctg gttccatgt tcccggctt tacttttca 
      tgggtttta ttctggaga ctgcatcaa gaccaccca 
      tcattttta ccaccatgg ggaccgcta gcatcacct 
      gcattggca ttttcttct ggacgaata ctttcgttg 
      tgacactta ttgaactaa cagtcaaaa ttatgctga 
      tgtgtgccg taaatgccg ttaacatgg tggtagtcg 
      aaggatacc cttgtcatt taagatgta ccgcccatg 
      gtatgatgc gcagtttat tccacttag cgtcccttt 
      gtgccatcc ccatctatg gtatgtttg gtcacacaa 
      tgttaatgt tccccgctg tgatcacca tttctttga 
      cttttgggg ccgctttgt tgtctttga agatacaag 
      acaacagta aagttctct tgtaatatt ttatctgtt 
      tgtagattg tgtctgtga tattatttt taaaacgat 
      aatcacatt gtcattcaa ctgttagat gagatagat 
      gcgtcaatt ataagcttt tattttcca aaatttcat 
      aaagttcaa gctattgca tatcaattc atgtatgcc 
      tgatgcata ggaaacaag tcaaattac aattcttgg 
      aaccctttt acatattga aggattgat aggtcgtat 
      aatattgcc ctcatggtt agtgaagag atcgaaatt 
      gtaatttat tttttattg ttgaacaaa taaatcccg 
      cttaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY