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Gene information
General information
TC031519 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4248991..4251205
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031519
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031519 RA
      MKAKCQSKK LPKDVNPKA VFACNELDL KEVNVYGFD 
      YDYTLACYK PSMDYVLYN LGRQTLIEQ YKYPEEIAQ 
      LEYKPGFAV RGLHYDIEK GILLKLDSF LQIQFGSVY 
      KGLTPLSVE EVLKIYKNR IIPIAYVEG NTKNSSLVL 
      QDSHSKMVQ LADLFSVPE MGLICNVAD YFERNHIDY 
      HPEILFRDV KSSVKSSHP VMHKIVSEN VSEYIESNE 
      NLRPFFDRL KSANKNLFL VTNSPYNFV NKGMEMLVG 
      RDWKDYFDV LIVQARKPR FFTDISRPI RVFDEQSGS 
      HIWDRVTKL EKGVIYYEG TVKQLQDLT GWRGHQVLY 
      FGDHPYSDL ADVTLEHGW RTGAIINEL THEIATLNN 
      VEFKKNANW LQMLTQLIE DHQDCEDPE EQKILQKWM 
      AERDKLRND IKFVFNPQF GSVFRTYHN PTYFSRRLF 
      RFADIYTSN ITNLLNYSV KHTFYPRRG VMPHEYISY 
      FV
    CDS sequence
    • >TC031519 RA
      atgaaggca aaatgtcaa tcgaagaaa ttaccgaaa 
      gacgtcaac ccaaaggcc gtttttgcg tgtaatgag 
      ctagattta aaggaagtt aacgtttat gggtttgat 
      tatgactat acgcttgct tgctacaag ccctcaatg 
      gactatgtt ttatataat ttaggtaga cagacgctt 
      atagaacag tataaatac cctgaagaa atagcacaa 
      ttggagtat aagccaggc tttgccgtc aggggacta 
      cattatgac atagaaaaa ggaatttta ctcaaattg 
      gattctttt ttacaaatc caattcggc tcagtttac 
      aaagggcta acgccgcta tccgtggaa gaggttctg 
      aaaatttac aagaaccga attatccct atagcttac 
      gttgaagga aacacaaaa aattcctcg ttggtatta 
      caggacagc cactctaag atggtgcaa ctggctgat 
      ttattctct gtacctgaa atgggtttg atttgcaat 
      gtggcagat tatttcgag cgtaatcac attgattac 
      cacccagaa attttattt agggacgtt aaaagttcc 
      gtcaaaagc tctcatcct gttatgcac aaaatagtc 
      tcggaaaac gtatcagaa tacatagag tcaaacgaa 
      aatctcagg ccgtttttt gatcgctta aaaagcgca 
      aataagaat ttatttcta gtcaccaat agtccttat 
      aatttcgtt aacaaaggt atggaaatg ctggttggg 
      cgcgactgg aaagactac ttcgatgtt cttatcgtt 
      caagccagg aaaccccgg tttttcacc gacatttcg 
      cggcccatt cgtgtattt gacgaacag agcggcagc 
      cacatttgg gatcgtgtg acaaaattg gaaaaagga 
      gttatttat tatgaagga actgtaaaa cagttacaa 
      gacttaact ggctggcgt ggtcatcaa gtcttgtat 
      tttggggac cacccttac agtgacctc gctgacgta 
      acgttagag catggatgg aggaccggg gccataatc 
      aacgagttg acacacgaa attgccacc ttaaataac 
      gtggagttc aagaaaaat gccaactgg ttgcaaatg 
      ttgactcag ctgattgaa gaccaccaa gattgtgaa 
      gatccagaa gaacagaaa attcttcaa aaatggatg 
      gctgaacgc gataagttg agaaatgat attaagttt 
      gtgttcaat ccccagttt gggagcgtc ttccgcaca 
      tatcacaac cctacgtat ttttctaga cgattattt 
      agatttgcc gacatatac acgtcgaac ataactaat 
      ttattgaat tactcggtc aagcacacg ttttatcca 
      agacgcggt gtcatgccc cacgagtat atatcctac 
      tttgtatag
    mRNA sequence
    • >TC031519 RA
      ttgttaaaa aaaaacagt caatcgtaa tgttattat 
      gcaagctca ttaactgtt atccaagtt tacgcttaa 
      caggccatt atttaacgc agcaaaaat gggtcgttt 
      caaaaaaaa attcaagat ttcattcaa ctcaacttc 
      tagaagatg cgtacaaga aaatgaagg caaaatgtc 
      aatcgaaga aattaccga aagacgtca acccaaagg 
      ccgtttttg cgtgtaatg agctagatt taaaggaag 
      ttaacgttt atgggtttg attatgact atacgcttg 
      cttgctaca agccctcaa tggactatg ttttatata 
      atttaggta gacagacgc ttatagaac agtataaat 
      accctgaag aaatagcac aattggagt ataagccag 
      gctttgccg tcaggggac tacattatg acatagaaa 
      aaggaattt tactcaaat tggattctt ttttacaaa 
      tccaattcg gctcagttt acaaagggc taacgccgc 
      tatccgtgg aagaggttc tgaaaattt acaagaacc 
      gaattatcc ctatagctt acgttgaag gaaacacaa 
      aaaattcct cgttggtat tacaggaca gccactcta 
      agatggtgc aactggctg atttattct ctgtacctg 
      aaatgggtt tgatttgca atgtggcag attatttcg 
      agcgtaatc acattgatt accacccag aaattttat 
      ttagggacg ttaaaagtt ccgtcaaaa gctctcatc 
      ctgttatgc acaaaatag tctcggaaa acgtatcag 
      aatacatag agtcaaacg aaaatctca ggccgtttt 
      ttgatcgct taaaaagcg caaataaga atttatttc 
      tagtcacca atagtcctt ataatttcg ttaacaaag 
      gtatggaaa tgctggttg ggcgcgact ggaaagact 
      acttcgatg ttcttatcg ttcaagcca ggaaacccc 
      ggtttttca ccgacattt cgcggccca ttcgtgtat 
      ttgacgaac agagcggca gccacattt gggatcgtg 
      tgacaaaat tggaaaaag gagttattt attatgaag 
      gaactgtaa aacagttac aagacttaa ctggctggc 
      gtggtcatc aagtcttgt attttgggg accaccctt 
      acagtgacc tcgctgacg taacgttag agcatggat 
      ggaggaccg gggccataa tcaacgagt tgacacacg 
      aaattgcca ccttaaata acgtggagt tcaagaaaa 
      atgccaact ggttgcaaa tgttgactc agctgattg 
      aagaccacc aagattgtg aagatccag aagaacaga 
      aaattcttc aaaaatgga tggctgaac gcgataagt 
      tgagaaatg atattaagt ttgtgttca atccccagt 
      ttgggagcg tcttccgca catatcaca accctacgt 
      atttttcta gacgattat ttagatttg ccgacatat 
      acacgtcga acataacta atttattga attactcgg 
      tcaagcaca cgttttatc caagacgcg gtgtcatgc 
      cccacgagt atatatcct actttgtat aggctttta 
      ggcaaaaac tctattgtg tcattttaa ttttataga 
      caagaaact attgtgtca tattaattt tataggaaa 
      tgcatacat gttgtatct gctgcgagt aaaaaatat 
      t
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