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Gene information
General information
TC009451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1007860..1012104
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009451
    iBeetle screen iB_10246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009451 RA
      MSQSRSKKP SMPTNRPKP HMPVIDEKM CFKAVKKPK 
      EFVTAYDVQ GMRRLMVPD RNPIDIPGI LPKSEFERL 
      KNQAHIVTL KDRMMMLDE AEQKKNALQ RESEKRKEF 
      LQKVQKKQT AKPGTTLSD VEREAKSRN MYLLKRSFD 
      LMQEQDNRV KRANGVILA TKCRAIRNA QIAEKELIE 
      KQLQEENRR LDLMMEQQR QKAMEEEAK RKQEEERKK 
      QNYVTALSD QMRMNEIER MIEAEQKEE ESRMINKAF 
      LAIQQEEEQ KLREKKELQ QKIRNDLKK ANDDLERYK 
      LIQKEEQRI ADLRIQKFM QEKLDREMA RDQELALAK 
      AAKEQEIIR MRNQQQRSQ DIKAAMDEM NALRIQEEV 
      ERKWREKEI AAAKAKKEQ QEMLKLERE KQINDLRRA 
      AAITLARDE EDFHRVAKV QREQWEKEM ELNKRKKEK 
      AEQHRTELL KQINEKEKE RIVVQKEKF EEANLQQIE 
      QQRKDLHVK EHLRQKIEA LRNKQLPER YIKDIEHHI 
      EGIINTE
    CDS sequence
    • >TC009451 RA
      atgtctcag agtcgttca aaaaagccc tcaatgccc 
      acaaaccgg cccaaacct cacatgccc gtcattgac 
      gagaaaatg tgtttcaaa gcggtcaag aaacccaaa 
      gaattcgtt acagcgtac gatgttcaa gggatgagg 
      aggctcatg gtccctgat agaaaccca attgacata 
      ccggggatt ttacccaaa tctgaattc gaaagacta 
      aaaaatcag gcacatatt gtgactcta aaagataga 
      atgatgatg ttggacgag gcggaacaa aagaaaaac 
      gccttgcaa cgagagagt gagaagaga aaagaattc 
      ttgcaaaaa gttcaaaag aaacaaacc gcaaaacct 
      ggaacaacg ttaagtgac gtcgaacgc gaagcgaaa 
      tctcgaaac atgtatctt ttgaaacgc tctttcgac 
      ttaatgcag gaacaagac aatagggta aaacgagcc 
      aatggtgtg attcttgcg acgaaatgt cgtgcaatc 
      agaaatgca caaattgcc gaaaaggaa ctgatagag 
      aaacaactg caagaagaa aaccgccgt cttgaccta 
      atgatggaa cagcagcgc cagaaagca atggaagaa 
      gaagcaaaa cgcaaacaa gaagaagaa aggaagaag 
      caaaactac gtgactgct ttatccgac caaatgcgc 
      atgaacgaa atcgaacga atgatcgaa gccgagcag 
      aaggaagaa gagagccga atgatcaac aaggcgttc 
      cttgccatc caacaggag gaagaacaa aagttgagg 
      gaaaaaaag gagttgcag caaaaaatc cgaaacgat 
      ttgaagaaa gcaaacgat gatttagaa cgctacaaa 
      ttgatccag aaagaggag cagagaatc gccgatttg 
      aggatccaa aagttcatg caagaaaaa ctagatcgt 
      gaaatggcc cgcgaccaa gaactggcg cttgccaag 
      gcggccaag gagcaggaa atcataaga atgcgaaac 
      cagcagcaa agatcgcaa gacatcaaa gcggcaatg 
      gacgaaatg aacgccttg cgcatccag gaagaggtc 
      gaacgcaag tggagagaa aaggaaatt gcggctgcc 
      aaagccaaa aaggagcag caagaaatg ttgaagctt 
      gaaagagag aagcagatt aacgatttg aggagagca 
      gcagctatc acgttggca cgggacgag gaagatttt 
      cacagagtg gccaaagtg caaagggag cagtgggag 
      aaggaaatg gaattgaac aagaggaag aaggagaag 
      gcggaacaa caccgcacg gagttactc aagcaaatt 
      aatgagaag gagaaggag aggattgtg gttcagaag 
      gagaagttt gaggaagct aatttacaa caaatcgaa 
      cagcagagg aaggatttg catgttaag gagcatttg 
      aggcaaaag attgaggcg ttgaggaat aagcaatta 
      ccggaacgg tatatcaag gatattgag catcatatt 
      gaaggcatt attaatacg gaatga
    mRNA sequence
    • >TC009451 RA
      tatgttttg tacatgcgt attttattt gttttgtgt 
      ggtgtgacg ctgcgacat atcagtttt ttcttatat 
      catttacag attttaaat gtggtcccc aattactaa 
      tggtcaagc atctgcggg agacatttt tcttagaac 
      ctttttcat cttagttat aagcgacta attttatca 
      ggagcaaac gtgccgccg ccgtctttg aaacaaatc 
      aacggaaaa tgcatattt agattataa agttgtaga 
      ataaggttt tgttcggaa tcattttta tttttcttc 
      ggtcatttc gtttgccat tacgcagta tttgataag 
      gatatttgc aaatacatc atgtacaca accttgcga 
      gcttttatt taactggaa agtgtttgg attgtttgc 
      ttttgatga tgaaaacta tgattagtg acttgtcta 
      gcttccaat ctataccat aaaattttg tagtgaata 
      ggttataat tacctgtgt gttagcttg ttatttata 
      gggtatgtc aggataagt gggacggct ttcccggag 
      gtcgctgca acagttatg gtaaggcta tcgctgtaa 
      atgccgatg tatgacttg tatatgctc actgttaaa 
      aatcgattg aaattcgct atgttcgaa ggagcgcac 
      acttccagc aagtgcaat aaccattcc ttcgtctag 
      ttaagaggc ttccttcgg gcatagacg gcaaaactt 
      gtttactca cctaaacaa attttgcgc aacttcgtc 
      cagaacaaa taaaaatct caaatcgtt catttattc 
      tttccgaga gatgtgtat tcacttcgt ttttagttt 
      tgataatat tgtaaccca ctatgtacg gcagttaga 
      ctgcattgt attaattta cattgatta aacttaacc 
      tattaccac ttgtgtctg ttgtttttg ttatagttt 
      cagatcagc acgcaggta cagtatgcg attttaccg 
      acatttcgt tttattatt acactatcg ccctgtccg 
      atgtattct aggtgcctt cactatttg cggaaataa 
      aacatcgcg cgcaatcta tgttttatt tcagtcagc 
      gtgcggttg tgtaaattt tttgcttta taggtaaga 
      tattcttgt ctcaatatt tgtgtggac gctagattt 
      gtacttgat gatttgaac gagtaatct cacttctga 
      ttacttgtt tcaggtcca gacaacatc gtcttttat 
      ttagcactt gggttctgc ttgcttctc tagatttat 
      tcagtattt ttgcatgtc ttgtgatta ttttttcag 
      attcgccga acaagtgcc tagttcttc ctggtttta 
      tttttgatt tatcattcg gacaggaca cgtcctgtc 
      tcacatggt aagaaaaac ctacacaat agtattttg 
      ttcgttgtc ccatgttag agataagcg cagagtttg 
      accgatttt tacttgtat tctagatat ttagaagct 
      ttttacttt tgtacagct ttttaaaaa aataaacat 
      tttttttgc acttttttc atacttgaa ccccttatt 
      tcatcctgg tatcatcga gttattcat tgtgcggag 
      cgttgctat gactttatt tgatcaatt tttgacaca 
      ttgaagtga gaatgtctc agagtcgtt caaaaaagc 
      cctcaatgc ccacaaacc ggcccaaac ctcacatgc 
      ccgtcattg acgagaaaa tgtgtttca aagcggtca 
      agaaaccca aagaattcg ttacagcgt acgatgttc 
      aagggatga ggaggctca tggtccctg atagaaacc 
      caattgaca taccgggga ttttaccca aatctgaat 
      tcgaaagac taaaaaatc aggcacata ttgtgactc 
      taaaagata gaatgatga tgttggacg aggcggaac 
      aaaagaaaa acgccttgc aacgagaga gtgagaaga 
      gaaaagaat tcttgcaaa aagttcaaa agaaacaaa 
      ccgcaaaac ctggaacaa cgttaagtg acgtcgaac 
      gcgaagcga aatctcgaa acatgtatc ttttgaaac 
      gctctttcg acttaatgc aggaacaag acaataggg 
      taaaacgag ccaatggtg tgattcttg cgacgaaat 
      gtcgtgcaa tcagaaatg cacaaattg ccgaaaagg 
      aactgatag agaaacaac tgcaagaag aaaaccgcc 
      gtcttgacc taatgatgg aacagcagc gccagaaag 
      caatggaag aagaagcaa aacgcaaac aagaagaag 
      aaaggaaga agcaaaact acgtgactg ctttatccg 
      accaaatgc gcatgaacg aaatcgaac gaatgatcg 
      aagccgagc agaaggaag aagagagcc gaatgatca 
      acaaggcgt tccttgcca tccaacagg aggaagaac 
      aaaagttga gggaaaaaa aggagttgc agcaaaaaa 
      tccgaaacg atttgaaga aagcaaacg atgatttag 
      aacgctaca aattgatcc agaaagagg agcagagaa 
      tcgccgatt tgaggatcc aaaagttca tgcaagaaa 
      aactagatc gtgaaatgg cccgcgacc aagaactgg 
      cgcttgcca aggcggcca aggagcagg aaatcataa 
      gaatgcgaa accagcagc aaagatcgc aagacatca 
      aagcggcaa tggacgaaa tgaacgcct tgcgcatcc 
      aggaagagg tcgaacgca agtggagag aaaaggaaa 
      ttgcggctg ccaaagcca aaaaggagc agcaagaaa 
      tgttgaagc ttgaaagag agaagcaga ttaacgatt 
      tgaggagag cagcagcta tcacgttgg cacgggacg 
      aggaagatt ttcacagag tggccaaag tgcaaaggg 
      agcagtggg agaaggaaa tggaattga acaagagga 
      agaaggaga aggcggaac aacaccgca cggagttac 
      tcaagcaaa ttaatgaga aggagaagg agaggattg 
      tggttcaga aggagaagt ttgaggaag ctaatttac 
      aacaaatcg aacagcaga ggaaggatt tgcatgtta 
      aggagcatt tgaggcaaa agattgagg cgttgagga 
      ataagcaat taccggaac ggtatatca aggatattg 
      agcatcata ttgaaggca ttattaata cggaatgat 
      gtgtaattg cattgaaat gacaaaata aagcacgga 
      caatcat
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