Status
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Gene information
General information
TC030699 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1022867..1029054
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030699
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    yolk late amount decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030699 RA
      MYSCSHLAT ATNIPIANI LNSIERPVC ADCGCTKPN 
      LWICLFSNC LQICCGEKF NDHSTIHNS KNPTHCIHM 
      NLVTKRIWC YECKGEVFM EQPSAVSST VPSYDSDPE 
      MEGCTYKSR DSGHGSYST LRTNTPERM NVFAFEKTV 
      GLNVGMSGD ASDASDAED STEYSIDRP VGLVGLQNI 
      GNTCYMNAA LQALSNAIP LTRFFLECA TTVQILSEG 
      KKPGLSRTY QTLIRDMWK KNGGYVTPS GILYGIRSV 
      YPMFRGYYQ HDTQEFLRN FMDQLHEEL KQVAPPEVA 
      TMTDSDTFS LAMDEPALS SSYDSSEGE YETCDSGVS 
      ERSSLSDDT ERPASSTKR RLSRSSSPG RRIRTRLQS 
      NTMVIDSQP STSSSSASI NKKQQKYRS IISDIFDGK 
      LLSSVQCLT CDRVSSCVE TFQDLSLPI PSRDHLVVL 
      HGRTTGPTA TCSEAVIPM NEGWISWIL AWLKSWFYG 
      PVVTLHDCL AAFFSTDEL KGDNMYSCE KCNKLRNGI 
      KFSKVLQLP EVLCIHLKR FRHELMFSS KISSAVSFP 
      LKGLDMRPY LHTDCISKV TTYELFSVI CHYGTAGGG 
      HYICYALNG GQWYEFDDQ YVTRVPPEK VQSCEAYVL 
      FYRKVTNTA DEIRSKAIK LAKTHSTEP HEVAYISKQ 
      WISRFNTCA EPGAIDNSD FLCQHGSFH PDREIVFER 
      LAVALPLPV YEYLHKKFG GCPPVTSIN ICPACLAFN 
      NRLLFEMET FVQLNREMQ NKDVPLTHL ISTSWYTQW 
      HKFVQKRAA DAPGPIDNS KINMNQIDV NECAEITEG 
      IWNFFFNIY GGGPELRLR PKSLHRTES ESSLPSDGG 
      EQTKFVIPA LKKEKVDTI DKSVYCSGE PMEVEEIEG 
      ENECNGFAE EGDSPSDTL PNGICAISH SKLANVDND 
      VSDDEVCNV EVKNSKRHR RRRKALSNI N
    CDS sequence
    • >TC030699 RA
      atgtacagc tgtagccat ttagcgaca gcgacgaac 
      attcccatc gcaaacatc ctcaattcg atcgagagg 
      cccgtctgt gccgactgt ggctgcacg aaaccgaac 
      ttatggatc tgcctgttt tccaactgt ttgcagatc 
      tgttgtggg gagaaattc aacgatcac agcactatc 
      cataattcc aaaaacccc acacattgc attcacatg 
      aacctcgta acgaaacga atatggtgt tacgagtgc 
      aaaggcgaa gtcttcatg gagcaacca tctgccgtt 
      tcctccacc gttccctca tacgacagc gatccagaa 
      atggaaggt tgcacctac aaatcgcgc gattccggt 
      catggcagc tacagcacc cttcgtacg aacactcca 
      gaacgaatg aacgtcttc gctttcgaa aaaacagtc 
      ggtcttaac gtcggaatg agcggagat gcgtcggac 
      gcaagcgat gccgaagac tcgacagag tacagcatt 
      gatagaccg gtcggtctg gtcggtcta caaaatatc 
      ggaaacacg tgttatatg aatgcagcg ttgcaagct 
      ttaagcaat gccattcct ttgacacga ttcttcctc 
      gaatgtgcc accacagtc cagatctta tccgaaggg 
      aagaaaccg ggtctgagc aggacttac cagactcta 
      atccgggat atgtggaag aagaacggg ggttatgtc 
      accccgtct gggatttta tacgggatc aggagtgtc 
      tatccaatg tttcggggt tattaccaa catgacact 
      caggagttt cttcgcaat tttatggat cagttgcat 
      gaagagttg aaacaggtg gcaccacct gaggtggct 
      acgatgacc gattcggac acttttagt ctagcgatg 
      gacgaacct gcgctcagt tcgagctat gattcgagc 
      gaaggggaa tatgaaacg tgtgattct ggggttagt 
      gaaaggtct agtctgagc gatgatacc gaaagaccg 
      gcaagtagc acgaagagg aggctgagt cggtcgtcc 
      agtccgggg aggaggata cggacgagg ctacaaagc 
      aacactatg gtgatagac tcgcagccg agcacgagt 
      tcaagcagc gccagtata aacaagaaa cagcagaaa 
      taccgctcg atcatttcg gatattttc gatggcaag 
      cttttatcg tccgttcaa tgtttgact tgtgatcgc 
      gtttcaagt tgtgtcgaa acattccaa gacctttcc 
      cttcccata ccgtcacgg gaccatttg gttgtgctt 
      catggtcgt acgacaggt ccaacagcg acatgttca 
      gaggccgta attccaatg aacgaaggt tggatttca 
      tggattttg gcgtggctg aagtcttgg ttttacggc 
      cctgttgtg acactgcac gattgttta gcggccttt 
      ttcagcacc gatgaattg aaaggggac aacatgtac 
      agttgtgag aagtgcaac aagttacgg aacggtatt 
      aagtttagt aaagtgctc caattaccc gaagtactt 
      tgtattcac ttgaagcga tttcgacac gagttgatg 
      ttcagtagt aaaatttct tcggcggtc agttttccc 
      ttaaagggt ttagacatg cgaccgtat cttcatacc 
      gattgtatt tcgaaagtt acaacttat gaattgttt 
      tcggttatt tgtcattat ggtacagcc ggtggtggg 
      cattatatt tgttatgcg ttaaacggt gggcaatgg 
      tacgaattc gatgatcag tatgtaact cgggtgcca 
      cccgaaaaa gtccagtcg tgtgaggcc tatgttttg 
      ttttaccga aaagtgacc aatacggcc gacgaaatt 
      aggagtaag gcgatcaag ttggcgaaa acgcattcc 
      acggaaccg cacgaggtg gcctacatt tctaaacag 
      tggataagc agattcaat acttgtgcg gaaccgggc 
      gccatcgac aactcggat tttctgtgc caacacggc 
      tccttccac ccggaccgc gaaatcgtg ttcgaacgt 
      ctagctgta gctttacct ttgcccgtc tacgaatac 
      ctccacaaa aaattcggg ggttgtccc cctgtcaca 
      agtatcaac atttgtccg gcgtgtcta gccttcaat 
      aaccgactt ttattcgaa atggaaact ttcgttcaa 
      ctaaaccgc gaaatgcaa aacaaggat gttccttta 
      acacatctc atttccact tcttggtac acgcagtgg 
      cacaagttc gtccagaag cgagccgcg gatgcgccg 
      ggtcctatc gacaactcg aaaatcaac atgaatcaa 
      attgacgtg aacgaatgt gcggaaata accgaaggg 
      atttggaat ttcttcttt aatatttat gggggaggg 
      cctgagttg aggctccgt ccgaagagt ttgcatcgg 
      acggagagc gagtcaagt ctgccgagc gatggagga 
      gaacaaacg aagtttgtg ataccagct ttgaagaaa 
      gagaaagtt gatacgatt gataagagt gtttattgt 
      tcgggggaa cccatggag gtcgaggag attgaaggg 
      gagaatgag tgtaatgga tttgcggaa gaaggggat 
      tcacctagt gatacttta cctaacggt atttgtgct 
      ataagtcat tctaaatta gctaacgtt gacaacgac 
      gttagtgac gacgaagtg tgtaatgta gaagttaaa 
      aatagcaaa aggcacagg agacgaaga aaagctttg 
      tcgaacatt aattaa
    mRNA sequence
    • >TC030699 RA
      taataacaa ataatgaca gaagaaacg tgaaacgtc 
      agtgtcatt ctcattagg atttttgtc aataaatca 
      aaaatatca tgtaataat aatttaatt tagctttgt 
      cctttatta tttttgtgt gtgaaagcg tattttggg 
      cggaggcgg ttcagtggt ggttttggt tgaaaaaag 
      ctaaaatgt acagctgta gccatttag cgacagcga 
      cgaacattc ccatcgcaa acatcctca attcgatcg 
      agaggcccg tctgtgccg actgtggct gcacgaaac 
      cgaacttat ggatctgcc tgttttcca actgtttgc 
      agatctgtt gtggggaga aattcaacg atcacagca 
      ctatccata attccaaaa accccacac attgcattc 
      acatgaacc tcgtaacga aacgaatat ggtgttacg 
      agtgcaaag gcgaagtct tcatggagc aaccatctg 
      ccgtttcct ccaccgttc cctcatacg acagcgatc 
      cagaaatgg aaggttgca cctacaaat cgcgcgatt 
      ccggtcatg gcagctaca gcacccttc gtacgaaca 
      ctccagaac gaatgaacg tcttcgctt tcgaaaaaa 
      cagtcggtc ttaacgtcg gaatgagcg gagatgcgt 
      cggacgcaa gcgatgccg aagactcga cagagtaca 
      gcattgata gaccggtcg gtctggtcg gtctacaaa 
      atatcggaa acacgtgtt atatgaatg cagcgttgc 
      aagctttaa gcaatgcca ttcctttga cacgattct 
      tcctcgaat gtgccacca cagtccaga tcttatccg 
      aagggaaga aaccgggtc tgagcagga cttaccaga 
      ctctaatcc gggatatgt ggaagaaga acgggggtt 
      atgtcaccc cgtctggga ttttatacg ggatcagga 
      gtgtctatc caatgtttc ggggttatt accaacatg 
      acactcagg agtttcttc gcaatttta tggatcagt 
      tgcatgaag agttgaaac aggtggcac cacctgagg 
      tggctacga tgaccgatt cggacactt ttagtctag 
      cgatggacg aacctgcgc tcagttcga gctatgatt 
      cgagcgaag gggaatatg aaacgtgtg attctgggg 
      ttagtgaaa ggtctagtc tgagcgatg ataccgaaa 
      gaccggcaa gtagcacga agaggaggc tgagtcggt 
      cgtccagtc cggggagga ggatacgga cgaggctac 
      aaagcaaca ctatggtga tagactcgc agccgagca 
      cgagttcaa gcagcgcca gtataaaca agaaacagc 
      agaaatacc gctcgatca tttcggata ttttcgatg 
      gcaagcttt tatcgtccg ttcaatgtt tgacttgtg 
      atcgcgttt caagttgtg tcgaaacat tccaagacc 
      tttcccttc ccataccgt cacgggacc atttggttg 
      tgcttcatg gtcgtacga caggtccaa cagcgacat 
      gttcagagg ccgtaattc caatgaacg aaggttgga 
      tttcatgga ttttggcgt ggctgaagt cttggtttt 
      acggccctg ttgtgacac tgcacgatt gtttagcgg 
      cctttttca gcaccgatg aattgaaag gggacaaca 
      tgtacagtt gtgagaagt gcaacaagt tacggaacg 
      gtattaagt ttagtaaag tgctccaat tacccgaag 
      tactttgta ttcacttga agcgatttc gacacgagt 
      tgatgttca gtagtaaaa tttcttcgg cggtcagtt 
      ttcccttaa agggtttag acatgcgac cgtatcttc 
      ataccgatt gtatttcga aagttacaa cttatgaat 
      tgttttcgg ttatttgtc attatggta cagccggtg 
      gtgggcatt atatttgtt atgcgttaa acggtgggc 
      aatggtacg aattcgatg atcagtatg taactcggg 
      tgccacccg aaaaagtcc agtcgtgtg aggcctatg 
      ttttgtttt accgaaaag tgaccaata cggccgacg 
      aaattagga gtaaggcga tcaagttgg cgaaaacgc 
      attccacgg aaccgcacg aggtggcct acatttcta 
      aacagtgga taagcagat tcaatactt gtgcggaac 
      cgggcgcca tcgacaact cggattttc tgtgccaac 
      acggctcct tccacccgg accgcgaaa tcgtgttcg 
      aacgtctag ctgtagctt tacctttgc ccgtctacg 
      aatacctcc acaaaaaat tcgggggtt gtccccctg 
      tcacaagta tcaacattt gtccggcgt gtctagcct 
      tcaataacc gacttttat tcgaaatgg aaactttcg 
      ttcaactaa accgcgaaa tgcaaaaca aggatgttc 
      ctttaacac atctcattt ccacttctt ggtacacgc 
      agtggcaca agttcgtcc agaagcgag ccgcggatg 
      cgccgggtc ctatcgaca actcgaaaa tcaacatga 
      atcaaattg acgtgaacg aatgtgcgg aaataaccg 
      aagggattt ggaatttct tctttaata tttatgggg 
      gagggcctg agttgaggc tccgtccga agagtttgc 
      atcggacgg agagcgagt caagtctgc cgagcgatg 
      gaggagaac aaacgaagt ttgtgatac cagctttga 
      agaaagaga aagttgata cgattgata agagtgttt 
      attgttcgg gggaaccca tggaggtcg aggagattg 
      aaggggaga atgagtgta atggatttg cggaagaag 
      gggattcac ctagtgata ctttaccta acggtattt 
      gtgctataa gtcattcta aattagcta acgttgaca 
      acgacgtta gtgacgacg aagtgtgta atgtagaag 
      ttaaaaata gcaaaaggc acaggagac gaagaaaag 
      ctttgtcga acattaatt aactcagat tttttgtac 
      ataaaggtt tgtatgtta ttgaagcaa taaatgttg 
      ggtattttt atcgactat tgttgatta atgggagtc 
      actagtaca aaaattgct accgggagg ctattaaag 
      attgtgtca attgacaca gtttttaaa aaccactct 
      gtattttca aatgccagg ccagtaaag cttgatttt 
      tggctccca ttagttgat ggcaacctt ttgacagtt 
      atttaaact ggcgccctc ggtccatcc agtggtacc 
      gactgttaa cacattttt tcgttggcc aattgtctg 
      atgaggtga atttttgtg gctcctggc ggcccgttt 
      tttgttttt cgctgaaaa cttccaaga aatcgttgg 
      aaaaaagtg atacataat tttcgtttt taagtttcg 
      atggattca ttaaactta taataacca ctcataatt 
      attttttgt tatcgaatt tctgccgta aattttaat 
      gacagcgat caacgaaaa aatgtaatc aactcattt 
      gctaatggg cgattaatt ccgctattt gagcatcgc 
      tgattaatc gccccgtta acaagtcag tttattgaa 
      taaaaatag cacagggtc tacttatca attggtttg 
      tttgcattt agtattttt gacgcgggc ggaatgtga 
      taagttcgt ttagtaaga tcgtgtatt tgtttaatt 
      gtcgacgtt gtgttaatt tattaaacg gcgacaatc 
      actgattaa gttacgcca accttgctc tatttaagt 
      gggtgaaaa gtggagtgt gggttataa aaattaagt 
      atttttgaa ataaaaaag ccaaactaa ttttaagaa 
      agtgtatat agtgtgtaa tacaaagcg taagtgggg 
      caaatattt ttccatgcc aaaaaatct tatatattt 
      gtgcaatta ttaagagaa atatagaaa ttttactcc 
      tgtaattgt acatagtct aattttgtt tgtatcagg 
      tttatgtag gtgttatta cataagtca cttattagg 
      aacattgta ataatggtt acgttgctt gagttcctg 
      gctcgtcca acggacagg attttaaac aattatttc 
      aattattat tgttataga ataaaacat catttttca 
      t
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