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Gene information
General information
TC009361 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1032422..1054876
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009361
    iBeetle screen iB_10244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009361 RA
      MHKVWSWLI LGIAVSQVL SLRLERSPS PSTTPTPDD 
      DDDEWDDDD SSEIEDDGK IYKNPRNSP STLCPRDDA 
      QATQLGHRC LRKCSSDED CKSKKKKCL CDGACGMSC 
      IKPDRECPE PKQIPFGSV SYTERVFGS KATYSCQHG 
      YHVVGLQSR TCQADGKWA GSAPVCKQN IYCLSPPTI 
      DHARHNALP EQTTFDLDS TLQYYCHTG YVTNGFPRA 
      KCLAIDGQA SWYGPDISC EPNSCGAPT DISHGWHAG 
      ECYTYGCRI TYHCAEGYE LVGKNERFC QADGTWTPK 
      ELPTCVPVQ CSPPENPRH GKAIYTSCS YNSVVSYEC 
      KYGYTLQGE SSRRCGADR KWSGAQPIC KEINCGHPG 
      RLWNGWLEN IEGGTGLGA SIIFRCHEG MLLVGNTST 
      VCQSDGKWR YPLPKCLAP CVVPHVSQG RVILVSKND 
      TAPTSTVVQ HGEALEVNC EYQYEFLAS TSAVTCNNG 
      TWTHIPKCE PARCKHLPK PPKNGMVIA PKMNHNMKA 
      RFKCKDGFQ IKGHPLIEC SFGNWTGEI PKCEEVYCP 
      YPGSVSNGK ILLVGNMGL YDYRPYVRK VTNNKQIMY 
      DCDKGYVLA EGPPGATCI GGIWSPSQL PKCILGQHP 
      RLRWNRRRR SIVMEKYKR AFIRQNKLI YQNREKRHV 
      HNYLNYLKE QHREGAKMR LKRALKGGS SSRAFGTSH 
      KSSNSQLSL RRGNKKNYN YYEEEKQEE IKKPKHKSR 
      GPCEPLSSE PYVNIEIVK HGRDHNVSF SSGTIVKMA 
      CGKGYGLNM PENKTAKCV RGRWRPTKP TCSILPCFV 
      PSIENGVFK LSKAELPSN ITFDLDEPL NETVEIGNG 
      QVVEFSCLE GYNIQGPSN LRCWHGDWT VTGLPECTA 
      SPCQLPQIT NGQYLMGYR AGLTIANGS SVTFQCDSD 
      YSKSTAQPI QCILGELYP KSPSCRPEA GVDLNSKVI 
      DSPHFIGGA DIIKGGDIT VLQYGSPSK ACGPPAKVQ 
      GSLVYKNGE PIVDDENNF PDGTEVTFN CIETIMGEK 
      TTWKIVCED GSWIGRSLN CDTEELLAS QIVYNNNTC 
      IFRNQEPNV ISFYNDQQI REEVVEFPA GAVLVSRCV 
      DIGKYAMTG SNTRRCMGG DWDGTKPAC FGLNQENDY 
      SMEKPPTIL FRHQLGPIA QSNDGKLIV YPGTILHME 
      CLWIRRFGN PKWTVSHDY RKYPEGWTT DPGRDSQLE 
      YRLSIFHAS KDDSGLFTC VTPARYTHS VEIEVKAVH 
      CPIVPQRRG LTVNTQSTK MNTRLKFSC INGNALIGA 
      SEITCLPSG NWSAPFPVC ESIECGEVG GVLPSRLRV 
      LVVSREVGG RAVFSCEPG FGLRGPQET VCQPNGDWA 
      TPFPTCEEV QCDNPGSPE NGYMQGSGP FKAGDVVQF 
      NCNPDFMME GQPIIACQD NSRWSGKLP KCVQACSYP 
      GTTISGKMS SVKFYYKIG ENITFTCDE GLTLKGAAM 
      LKCLKNGKW SNAIPTCVT ENNTPEND
    CDS sequence
    • >TC009361 RA
      atgcacaaa gtgtggagt tggttgatt cttggcatc 
      gccgtgagc caagttctg tcgctgcga ctggagcgt 
      tcaccctcc cctagcaca accccaacc cccgacgac 
      gacgatgac gaatgggac gacgatgat tcgtccgaa 
      atcgaagat gacggcaag atttataaa aatccaagg 
      aactcgccc tcgacgctg tgcccccgg gacgacgcc 
      caagccaca caactggga cacagatgt ctgcgaaag 
      tgctcgtcg gacgaagac tgcaagagc aagaagaag 
      aagtgcctt tgtgacggc gcctgcggc atgtcctgc 
      attaaaccg gatagggaa tgtccagaa ccgaaacag 
      atccctttc gggagtgta agctacact gaaagagtc 
      ttcggttca aaagcgacc tattcgtgt caacatggt 
      tatcatgtt gtgggatta cagagtcga acgtgtcaa 
      gccgatgga aagtgggcc ggatcggcg ccagtttgc 
      aagcagaac atttactgt ttgtcgcct cccacaatc 
      gaccacgct cgtcacaat gcccttccg gaacaaacc 
      acttttgat ctggactcc actcttcaa tactattgt 
      cacaccggt tacgtgacc aatgggttt ccccgagcc 
      aagtgtctg gctatcgac gggcaagcc tcatggtat 
      ggcccagac atctcatgc gaaccgaac tcctgcggc 
      gcccccacg gacatatct cacggctgg cacgcagga 
      gaatgttac acgtacggc tgtcgaata acttaccac 
      tgcgccgaa ggttacgag ctagttgga aaaaatgag 
      cgtttctgc caagctgac ggcacctgg acgcccaag 
      gaattgcca acttgtgtt ccggtgcag tgcagcccc 
      ccggaaaat ccgcggcat gggaaagcg atttacacg 
      tcttgtagt tacaactcg gttgttagc tacgagtgc 
      aagtatggg tatactctc cagggggag agcagcagg 
      agatgtggg gcggatagg aagtggtcg ggggctcag 
      cccatttgt aaagagata aactgtggc caccctgga 
      cgcctgtgg aacggctgg ctggaaaat atcgaaggt 
      ggcaccggc ctgggtgct tccataatt ttccgatgc 
      catgaagga atgttactt gtgggcaac acatcaacg 
      gtgtgccaa agcgacgga aaatggcga taccccctc 
      ccaaaatgt ttagctccg tgtgtggta ccacacgtg 
      tcccaggga cgagttata ctagtctcg aaaaacgac 
      actgcacct acatcaaca gtggtccaa catggggaa 
      gctctggag gtgaattgt gagtaccag tatgagttt 
      ttggccagt acttcggca gtaacgtgc aataatggt 
      acttggaca catattccc aaatgtgaa ccggctagg 
      tgtaagcat ttgccaaaa ccgcccaaa aacggaatg 
      gtcattgcc ccaaaaatg aaccacaac atgaaggcg 
      aggttcaag tgcaaggac gggttccag atcaaggga 
      catccccta attgagtgc tcgtttggc aactggacc 
      ggggaaata cccaaatgt gaagaagtt tattgccca 
      tatcctggg tccgtctcc aatggtaaa attctcctc 
      gtgggtaat atggggctt tatgattac cggccatac 
      gtccgtaaa gtcacaaac aacaaacag attatgtac 
      gattgtgac aaaggctac gtcctggcc gaaggccct 
      ccaggtgcc acgtgtatt ggaggcatc tggagcccc 
      tcacaactc ccaaagtgt attcttggc caacacccc 
      cgattgcgt tggaaccgc cgcagaaga tccatcgtg 
      atggaaaag tacaaacgc gctttcatt cgccaaaac 
      aagctcatt taccaaaac cgtgaaaaa cgccacgtt 
      cacaactat ttaaattat ttgaaagag cagcataga 
      gaaggggca aaaatgcgc ctgaaacgt gcccttaag 
      ggtggaagc agtagccgg gcttttgga acgtcgcac 
      aaaagctcc aactcgcag ttgagttta cgcagaggc 
      aataagaaa aattacaat tactacgaa gaagagaaa 
      caggaagag atcaagaaa cccaaacac aagtcacgc 
      gggccgtgt gaacccctc tccagtgaa ccatacgtc 
      aatatcgag attgtcaaa catgggaga gatcataat 
      gtgtcgttt agttccgga actattgtt aagatggct 
      tgcgggaaa ggctacggg ctgaatatg cccgaaaat 
      aaaacggca aagtgtgtt aggggacgc tggaggcca 
      acgaaaccg acctgctct attttgcct tgttttgtc 
      ccaagtatc gaaaacggg gtcttcaag ctgtccaaa 
      gccgagctt ccatcgaac atcacattt gatttggac 
      gagcccttg aacgaaact gtggaaatc gggaatggt 
      caagtggtt gagtttagt tgcctggaa ggttacaac 
      atccaagga cccagtaat ctgaggtgt tggcacggg 
      gactggacc gtgaccggt cttcccgaa tgtacagcc 
      tcaccttgc cagttgccc caaatcacc aatgggcag 
      tatttgatg ggttatagg gccggtctg accattgct 
      aacggttcc agcgttacg tttcagtgt gacagtgat 
      tacagcaaa tcgacggca cagccgatt cagtgcatt 
      ttgggcgaa ttgtacccc aaaagtccc tcgtgccgg 
      ccagaggcc ggagttgat ctcaatagc aaagttatc 
      gactcgcct catttcatc ggaggtgct gatatcatc 
      aaaggtggt gatattaca gttttacag tacggttcc 
      ccgtctaaa gcgtgtgga ccaccggct aaagttcaa 
      ggttctcta gtttacaaa aatggagaa ccgattgtg 
      gacgacgaa aacaatttt cctgatggt accgaggtt 
      acgttcaat tgtattgag accataatg ggggagaaa 
      accacgtgg aaaattgtg tgtgaggat ggcagttgg 
      attggccga tctctcaac tgcgacact gaagagtta 
      ctagcctcc caaatcgtt tacaacaac aacacttgc 
      atttttcgc aatcaagaa ccaaacgta atctcgttt 
      tacaacgac caacaaatc cgcgaggaa gttgtggag 
      tttccagct ggggctgtt ctggtcagt cgatgtgtg 
      gatataggg aagtatgca atgacagga tccaacacg 
      aggagatgt atgggaggg gattgggat ggcactaag 
      cctgcctgt tttggattg aaccaggag aatgactac 
      tctatggag aagcctccc acaattttg ttcaggcac 
      cagttaggg cccattgct cagtcgaac gatgggaag 
      ttgattgtg tatccaggg accatttta cacatggaa 
      tgtctctgg atcaggagg tttgggaat ccgaagtgg 
      accgttagc cacgattat agaaaatat cccgaaggc 
      tggaccact gatccaggc cgcgactcc caactagag 
      taccgcctg tccatcttc cacgcatct aaagacgac 
      tctggcctg ttcacctgc gtcacccca gcccgatac 
      acccattct gtggaaatc gaagtcaaa gctgtccac 
      tgccccatc gtcccacaa cgccgcggc ctcaccgtc 
      aacacccaa agcaccaaa atgaacacc cgcttgaag 
      ttctcctgc atcaacggc aacgctctg atcggcgcc 
      tccgaaatc acctgtctt ccttcgggc aactggagc 
      gccccattc ccggtgtgt gaaagtatc gagtgcggg 
      gaggtggga ggtgtgttg ccttctcgc cttcgtgtt 
      ctggtcgtt tctagagag gtcgggggc cgtgctgtc 
      tttagctgc gagcccggg tttgggctc agaggcccc 
      caggagacc gtgtgccag ccaaacgga gactgggcc 
      actccgttc cctacgtgc gaagaagtt cagtgcgac 
      aatcccggg agtcctgag aatggatac atgcaaggg 
      agtgggcct tttaaggcg ggggatgtg gtccagttt 
      aactgcaat cctgatttt atgatggaa ggacagcct 
      attatagcc tgccaggat aactcgaga tggtcgggg 
      aaattgcct aaatgtgta caagcttgt tcctatcca 
      gggacgacc atcagtggg aagatgtcc tcagtcaag 
      ttctactac aaaataggg gagaatata accttcacg 
      tgtgacgaa ggactgact ttgaaggga gcggcgatg 
      ttaaaatgt ctaaagaat gggaaatgg tccaatgct 
      attcccaca tgtgtcacg gaaaataac acacccgaa 
      aatgactga
    mRNA sequence
    • >TC009361 RA
      cgtcgctag aaccggaag tgtgttttc caaatattg 
      acttcgagc tttcggcgt aaaattacg tgcggctgc 
      ggctcaaaa aaaattaca aaaattcat tttttttaa 
      tccaaacaa tgtgaaaat caaaaatgt aatttattt 
      gaaactctg caccaataa tgtaaaatc tgtgtgttt 
      ggaaacttt gtaaacatg cacaaagtg tggagttgg 
      ttgattctt ggcatcgcc gtgagccaa gttctgtcg 
      ctgcgactg gagcgttca ccctcccct agcacaacc 
      ccaaccccc gacgacgac gatgacgaa tgggacgac 
      gatgattcg tccgaaatc gaagatgac ggcaagatt 
      tataaaaat ccaaggaac tcgccctcg acgctgtgc 
      ccccgggac gacgcccaa gccacacaa ctgggacac 
      agatgtctg cgaaagtgc tcgtcggac gaagactgc 
      aagagcaag aagaagaag tgcctttgt gacggcgcc 
      tgcggcatg tcctgcatt aaaccggat agggaatgt 
      ccagaaccg aaacagatc cctttcggg agtgtaagc 
      tacactgaa agagtcttc ggttcaaaa gcgacctat 
      tcgtgtcaa catggttat catgttgtg ggattacag 
      agtcgaacg tgtcaagcc gatggaaag tgggccgga 
      tcggcgcca gtttgcaag cagaacatt tactgtttg 
      tcgcctccc acaatcgac cacgctcgt cacaatgcc 
      cttccggaa caaaccact tttgatctg gactccact 
      cttcaatac tattgtcac accggttac gtgaccaat 
      gggtttccc cgagccaag tgtctggct atcgacggg 
      caagcctca tggtatggc ccagacatc tcatgcgaa 
      ccgaactcc tgcggcgcc cccacggac atatctcac 
      ggctggcac gcaggagaa tgttacacg tacggctgt 
      cgaataact taccactgc gccgaaggt tacgagcta 
      gttggaaaa aatgagcgt ttctgccaa gctgacggc 
      acctggacg cccaaggaa ttgccaact tgtgttccg 
      gtgcagtgc agccccccg gaaaatccg cggcatggg 
      aaagcgatt tacacgtct tgtagttac aactcggtt 
      gttagctac gagtgcaag tatgggtat actctccag 
      ggggagagc agcaggaga tgtggggcg gataggaag 
      tggtcgggg gctcagccc atttgtaaa gagataaac 
      tgtggccac cctggacgc ctgtggaac ggctggctg 
      gaaaatatc gaaggtggc accggcctg ggtgcttcc 
      ataattttc cgatgccat gaaggaatg ttacttgtg 
      ggcaacaca tcaacggtg tgccaaagc gacggaaaa 
      tggcgatac cccctccca aaatgttta gctccgtgt 
      gtggtacca cacgtgtcc cagggacga gttatacta 
      gtctcgaaa aacgacact gcacctaca tcaacagtg 
      gtccaacat ggggaagct ctggaggtg aattgtgag 
      taccagtat gagtttttg gccagtact tcggcagta 
      acgtgcaat aatggtact tggacacat attcccaaa 
      tgtgaaccg gctaggtgt aagcatttg ccaaaaccg 
      cccaaaaac ggaatggtc attgcccca aaaatgaac 
      cacaacatg aaggcgagg ttcaagtgc aaggacggg 
      ttccagatc aagggacat cccctaatt gagtgctcg 
      tttggcaac tggaccggg gaaataccc aaatgtgaa 
      gaagtttat tgcccatat cctgggtcc gtctccaat 
      ggtaaaatt ctcctcgtg ggtaatatg gggctttat 
      gattaccgg ccatacgtc cgtaaagtc acaaacaac 
      aaacagatt atgtacgat tgtgacaaa ggctacgtc 
      ctggccgaa ggccctcca ggtgccacg tgtattgga 
      ggcatctgg agcccctca caactccca aagtgtatt 
      cttggccaa cacccccga ttgcgttgg aaccgccgc 
      agaagatcc atcgtgatg gaaaagtac aaacgcgct 
      ttcattcgc caaaacaag ctcatttac caaaaccgt 
      gaaaaacgc cacgttcac aactattta aattatttg 
      aaagagcag catagagaa ggggcaaaa atgcgcctg 
      aaacgtgcc cttaagggt ggaagcagt agccgggct 
      tttggaacg tcgcacaaa agctccaac tcgcagttg 
      agtttacgc agaggcaat aagaaaaat tacaattac 
      tacgaagaa gagaaacag gaagagatc aagaaaccc 
      aaacacaag tcacgcggg ccgtgtgaa cccctctcc 
      agtgaacca tacgtcaat atcgagatt gtcaaacat 
      gggagagat cataatgtg tcgtttagt tccggaact 
      attgttaag atggcttgc gggaaaggc tacgggctg 
      aatatgccc gaaaataaa acggcaaag tgtgttagg 
      ggacgctgg aggccaacg aaaccgacc tgctctatt 
      ttgccttgt tttgtccca agtatcgaa aacggggtc 
      ttcaagctg tccaaagcc gagcttcca tcgaacatc 
      acatttgat ttggacgag cccttgaac gaaactgtg 
      gaaatcggg aatggtcaa gtggttgag tttagttgc 
      ctggaaggt tacaacatc caaggaccc agtaatctg 
      aggtgttgg cacggggac tggaccgtg accggtctt 
      cccgaatgt acagcctca ccttgccag ttgccccaa 
      atcaccaat gggcagtat ttgatgggt tatagggcc 
      ggtctgacc attgctaac ggttccagc gttacgttt 
      cagtgtgac agtgattac agcaaatcg acggcacag 
      ccgattcag tgcattttg ggcgaattg taccccaaa 
      agtccctcg tgccggcca gaggccgga gttgatctc 
      aatagcaaa gttatcgac tcgcctcat ttcatcgga 
      ggtgctgat atcatcaaa ggtggtgat attacagtt 
      ttacagtac ggttccccg tctaaagcg tgtggacca 
      ccggctaaa gttcaaggt tctctagtt tacaaaaat 
      ggagaaccg attgtggac gacgaaaac aattttcct 
      gatggtacc gaggttacg ttcaattgt attgagacc 
      ataatgggg gagaaaacc acgtggaaa attgtgtgt 
      gaggatggc agttggatt ggccgatct ctcaactgc 
      gacactgaa gagttacta gcctcccaa atcgtttac 
      aacaacaac acttgcatt tttcgcaat caagaacca 
      aacgtaatc tcgttttac aacgaccaa caaatccgc 
      gaggaagtt gtggagttt ccagctggg gctgttctg 
      gtcagtcga tgtgtggat atagggaag tatgcaatg 
      acaggatcc aacacgagg agatgtatg ggaggggat 
      tgggatggc actaagcct gcctgtttt ggattgaac 
      caggagaat gactactct atggagaag cctcccaca 
      attttgttc aggcaccag ttagggccc attgctcag 
      tcgaacgat gggaagttg attgtgtat ccagggacc 
      attttacac atggaatgt ctctggatc aggaggttt 
      gggaatccg aagtggacc gttagccac gattataga 
      aaatatccc gaaggctgg accactgat ccaggccgc 
      gactcccaa ctagagtac cgcctgtcc atcttccac 
      gcatctaaa gacgactct ggcctgttc acctgcgtc 
      accccagcc cgatacacc cattctgtg gaaatcgaa 
      gtcaaagct gtccactgc cccatcgtc ccacaacgc 
      cgcggcctc accgtcaac acccaaagc accaaaatg 
      aacacccgc ttgaagttc tcctgcatc aacggcaac 
      gctctgatc ggcgcctcc gaaatcacc tgtcttcct 
      tcgggcaac tggagcgcc ccattcccg gtgtgtgaa 
      agtatcgag tgcggggag gtgggaggt gtgttgcct 
      tctcgcctt cgtgttctg gtcgtttct agagaggtc 
      gggggccgt gctgtcttt agctgcgag cccgggttt 
      gggctcaga ggcccccag gagaccgtg tgccagcca 
      aacggagac tgggccact ccgttccct acgtgcgaa 
      gaagttcag tgcgacaat cccgggagt cctgagaat 
      ggatacatg caagggagt gggcctttt aaggcgggg 
      gatgtggtc cagtttaac tgcaatcct gattttatg 
      atggaagga cagcctatt atagcctgc caggataac 
      tcgagatgg tcggggaaa ttgcctaaa tgtgtacaa 
      gcttgttcc tatccaggg acgaccatc agtgggaag 
      atgtcctca gtcaagttc tactacaaa ataggggag 
      aatataacc ttcacgtgt gacgaagga ctgactttg 
      aagggagcg gcgatgtta aaatgtcta aagaatggg 
      aaatggtcc aatgctatt cccacatgt gtcacggaa 
      aataacaca cccgaaaat gactgaatg ttttacaac 
      aacttaaat gtgatatca ttgtttaag cgaataaag 
      ggttacatt aatcatcct gcaactgtt acatattaa 
      gacatatct gttacatgt agaaatttt tataattct 
      aaaatgttt aataaactg ttagacgta ataattaca 
      atgtgaaac tcatatcac gtaagttac ataatcact 
      actcattag ctcgcttgt aaggatata actattaat 
      aaagcatta gtaaagta
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