Status
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Gene information
General information
TC009369 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1108296..1120702
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009369
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009369 RA
      MGNSCSSGQ AHKDKDNMS QRSEESGSY QVRASLPSE 
      KQQTLLNHI TPHSGVILE PSTAGQPPL QTAAPPPTA 
      PSTLTVSEK KTNISKVLK RVAASSISA DSMSLSSPP 
      GTQSPQGFD KLSDGHVPK EVSSLSEQL QNLIMGRVS 
      QAKTASRTR KIVIYVCAA DSQDCYVEK GVLHNSVYP 
      ELRAHCRSK GYELHIVDL HWKTLLEKQ QDHEFPELC 
      IGELTRQME VAYIIPVLF LNNSLGTPL LPITIESAD 
      FKMAMESAE NQSAQTLLS KWYQIDSDA QPPCYRLQP 
      ISYHIPGFK ETSPEERER AFEEWRTEI DKMLAVLIT 
      VFTQELRDT YLTTVVEQE VHNTVFMSQ ELAKRCIWI 
      NRVYTPTAK TPDNLSPGD AELYRRLNT LQKDLRNQL 
      AEKHIVRIP VKYVEGGLN PEIPEHAQY IAQVTAHLQ 
      KHLVEMMDS IIEEHQGKT MLKPSQGIE APLFEELNQ 
      QTSFCQKAA QCSINREKT INEIKTYIM GDNPVPLIL 
      YGPGGCGKT TLLARVAQC CQQWLPEAF LIFRFIGIS 
      AQSSTIEQL LSSITNQCS ILTYGHKCY CIHNIETYE 
      KILPNLLAA SCLQRPLIL ILDGIDQVR SYSSKSIEW 
      LPTKLPDNI KLVLSVSEG SDEFLQIGR KISPNTFIK 
      MPLLGEAEA KNILMSSVM QYNHSVNSK IQDCVLKSV 
      QECTLPLYS KVLAWQTSW WADKEHDIV PKGHVNDQI 
      SLMLEELET ILGVTQVQH ALAIITSTK HGVTDSEMI 
      DLLAFDDSF HSTTTYVPW APACLTWSR LNKHLAPFL 
      QWTLTGGVL GVQWRDNML RQAVAVRYK EHAKWANQM 
      IYDYYTGKW WASKPANVL ARLVSQETI LGKCYNRRK 
      LDELPFHYY QLNGEIENS PYISDITWI YDKVCGSNC 
      YQILEDINL QKSVKNELT LMLRDFIET HASVLNYDG 
      RQFYSHLYK YLEDKVRRK EISLESNST LAQVYNVAK 
      NPPVLSLIP LNAGQGKGD ASAPLFQYF DLVTRLPET 
      DQFVISVST SKEEISVWD VKKCAQVRV LKGIPHPTN 
      LIPIDQYRC IVLCRRELR IYNLDCGTF MTKLKGVMN 
      QKMPYFGLH DQSHLVALS RNRMYVNLM NLETGDCVT 
      TFKAGEDRF LNSLLVSGD GRVLVCGDE TQKPFPLLV 
      WNLQSRKLL YDLRIPHHD FITSLSAIT YEGSYVCCV 
      CHEIDEPNP NFIVVYDLQ SGTLFKKWK PSCNTVSLE 
      ISSQGGCVI SGLEDARIL VWDLITGNC RWSLCGHTA 
      PVSSLKLDP TGGLCLSTD SECRDRSIR LWDLNKGNL 
      VSVYTPTRN IAACELTSN GHYIVLALE GEEEVVILK 
      LTGLRMDLN EENETYGVA ENTGKTFEL KESDAC
    CDS sequence
    • >TC009369 RA
      atggggaat tcttgcagc agcggacag gctcacaag 
      gataaggac aatatgtcc cagaggtcc gaagagtct 
      ggtagctac caagtccgc gcaagttta ccatccgaa 
      aaacagcag actctcctc aaccacata acgccccat 
      tctggagtg atcctcgag cctagtacc gccggtcaa 
      cctcccctt caaaccgcc gctcctcca cccacagca 
      ccctcaaca ctaaccgtc tccgaaaaa aaaaccaac 
      atctccaaa gtcctcaaa agagtagca gcatccagc 
      atttctgcc gattccatg tctctgagt tcaccacca 
      ggcacacaa agtccccaa ggattcgat aagctatca 
      gatggacac gttccaaag gaggtctcc agcctctcc 
      gaacaactc caaaatttg ataatgggc cgagtgtct 
      caagccaaa actgcgtcc agaacgcgc aaaattgtc 
      atctatgtt tgcgccgca gactcgcaa gattgttac 
      gtggaaaag ggcgtcctc cacaacagt gtttaccca 
      gaactgagg gctcactgc cggtcgaaa ggttacgaa 
      ctacacatt gtggaccta cactggaag accctcttg 
      gagaaacaa caagaccac gaattcccc gagctttgc 
      attggagag cttacaagg caaatggaa gtcgcctac 
      atcattccg gtacttttc ctgaacaat tctctcgga 
      acacctctc ttgcccata accatcgaa agcgccgat 
      tttaagatg gcaatggaa agtgccgaa aaccagtca 
      gctcagacc ctcctctcc aagtggtac cagatcgac 
      agtgatgct caaccccct tgctaccgc ctccagcca 
      atttcctac cacatcccg ggcttcaag gaaacatcc 
      ccagaagag cgcgaacgc gctttcgaa gaatggcgc 
      accgaaatc gataaaatg ttggcggta cttatcacc 
      gtcttcacg caagaactg cgcgacact tatctcacg 
      acagtggtg gagcaagag gtgcacaac acggtgttc 
      atgagccaa gagttggcc aaacgctgc atttggatc 
      aaccgcgtc tacacaccc acggccaaa accccggac 
      aatttgagt ccgggggat gcagagctc taccggagg 
      ctcaacacg ctacagaaa gatctgaga aaccagttg 
      gccgaaaaa catattgtt aggattccg gtgaagtat 
      gtggaaggt ggtttgaat ccggagatt ccggaacac 
      gcgcaatac atagctcaa gtgactgct cacttacaa 
      aaacaccta gtggaaatg atggactcg attatcgaa 
      gaacatcag gggaaaaca atgctgaag ccgagtcag 
      gggatcgaa gcgccgctt ttcgaagaa ctgaaccaa 
      caaacgagc ttttgccaa aaggccgca caatgtagt 
      attaataga gagaaaacg attaacgaa attaaaacg 
      tatataatg ggagataac ccagttcct ctaatatta 
      tacggcccc ggtggttgt ggcaagact actctattg 
      gcccgagta gcgcaatgt tgccagcag tggctgccg 
      gaagccttc ctaattttt agattcatc ggtatcagt 
      gcccaatca agcacaata gagcaatta ctaagttcg 
      ataacgaat cagtgttcg attttaacg tatggacat 
      aaatgttac tgtatacat aatatagaa acttatgaa 
      aaaatcctt ccaaatctc ttagcagct agttgtctc 
      cagcgccct ttgatacta atcctcgac ggcatagac 
      caagtccgc tcttacagt tccaaatcg atcgagtgg 
      cttcccacc aaactcccc gacaacatc aagctggtg 
      ctctctgta tcagagggc agtgacgag ttcctgcaa 
      atcggcagg aaaatcagc ccgaacacc ttcatcaag 
      atgccgctt ctgggcgaa gccgaggcg aagaacatc 
      ctcatgtcc agtgtgatg caatacaat catagcgtt 
      aattctaaa atacaagac tgcgttctc aagtcggtt 
      caggagtgc acgctgccg ctctactcc aaggtgctc 
      gcctggcag acttcctgg tgggcggac aaggagcac 
      gacattgtc ccgaaagga cacgttaac gatcagatc 
      agtctcatg ctggaggag ctcgagacg attttgggc 
      gtcacgcaa gtccagcac gctcttgca ataattaca 
      agcaccaag catggggtc acagattcg gagatgatc 
      gatttgttg gcgtttgac gacagtttt cacagcaca 
      accacttac gttccttgg gcgccggct tgtttgact 
      tggtcgaga ctcaacaag cacctggcg ccctttttg 
      caatggacg ttgactggg ggggttttg ggggtgcaa 
      tggagggac aatatgctg agacaagcg gtggcagtg 
      cggtacaaa gaacacgcc aaatgggca aaccaaatg 
      atttacgat tattacacg ggcaaatgg tgggccagc 
      aagccagcg aacgtcctg gcacgtctc gtgtcacag 
      gagacgatt ctgggaaaa tgttataac aggcggaag 
      ctggacgag ctgccgttc cactattat cagttgaac 
      ggggagatc gagaactcg ccgtacatt tcggacatt 
      acgtggatt tacgacaaa gtctgcggc tcgaactgc 
      taccaaatc ctcgaggac attaatttg cagaaaagt 
      gtgaagaac gagctgact ttaatgttg cgagatttt 
      atagaaacc cacgccagt gtcctcaac tacgacggt 
      cgacagttc tattcgcac ttgtacaaa tacttagaa 
      gacaaagtt aggcgtaag gagattagt ttagagtcg 
      aattccacc ctagcccag gtgtacaac gtggcgaaa 
      aacccgcca gttttgtca cttatcccg ctaaacgct 
      ggacagggg aaaggcgat gcctctgcc ccccttttc 
      cagtacttc gatttggtc acgagacta ccggaaacg 
      gaccaattt gtcataagt gtgtccaca agcaaggag 
      gaaataagc gtttgggac gtgaaaaag tgcgctcaa 
      gtgcgcgtt ttgaaaggc attccgcat ccgaccaac 
      ctgatcccg attgaccaa tacaggtgc atagttctt 
      tgtcggagg gagttgagg atatacaac ctggactgt 
      ggtaccttc atgaccaaa ctgaagggc gtcatgaac 
      cagaaaatg ccttacttc gggcttcat gaccaaagc 
      cacttggtg gcacttagc agaaaccgg atgtatgtt 
      aatcttatg aacctggag actggcgat tgtgtcacc 
      acattcaag gctggggag gaccgcttt ctcaattcc 
      ctgctggtt tcgggggat gggagagtc cttgtgtgt 
      ggcgacgag acacaaaag ccgtttccg ctcctagtg 
      tggaacctc cagtccaga aaactatta tatgacctt 
      agaatacct catcacgat tttataact tcactgtcg 
      gctatcacg tacgaaggt agttacgtt tgctgtgtt 
      tgtcacgaa atagacgaa cccaatccg aatttcatc 
      gttgtgtat gatttgcaa agtggtact ttatttaaa 
      aaatggaaa ccgtcatgt aacacggtt tccttagaa 
      attagctca caaggcggc tgtgtcata tccggtctc 
      gaggacgct cgaatactc gtttgggac cttattact 
      ggtaactgt cgatggtct ttatgtggc cacaccgct 
      cccgtttcc tccctcaag cttgatcca acggggggt 
      ttgtgtttg tcgacggat tcggaatgc agagataga 
      tccatccgg ctttgggat ctcaataaa ggcaacttg 
      gtgtcggtt tatacacca accaggaac attgcagcg 
      tgtgaactg acgtccaac ggccactac atcgtcctg 
      gcccttgaa ggcgaggaa gaagtggta attttgaag 
      ctaacagga ctccgtatg gacctaaac gaggaaaac 
      gaaacgtac ggagtggcg gagaacact ggcaaaact 
      tttgaactg aaggagagt gatgcgtgc tga
    mRNA sequence
    • >TC009369 RA
      actgtgtgt gtgacatac atcaaggca gacatgcag 
      tcgagcgct ttcaaatac tactattta tttatgtcc 
      gtgtagtgt gtaaatgtg tagaaaatc accttcgaa 
      cagcaccgt ctcgactac gccaccacc aagtcgcaa 
      aactctccc gactttcaa aattcaatc gcgatgaag 
      tttgcgaaa acgttgcat ttttcgaaa tttttacat 
      gttttcgag tgacgcgaa aatgatgcc acaaatcgt 
      gattatttc gaatggtgg aggcgtcga tctgattta 
      ctatgagaa gggtgtgaa ttgaaggag acatcatgg 
      ggaattctt gcagcagcg gacaggctc acaaggata 
      aggacaata tgtcccaga ggtccgaag agtctggta 
      gctaccaag tccgcgcaa gtttaccat ccgaaaaac 
      agcagactc tcctcaacc acataacgc cccattctg 
      gagtgatcc tcgagccta gtaccgccg gtcaacctc 
      cccttcaaa ccgccgctc ctccaccca cagcaccct 
      caacactaa ccgtctccg aaaaaaaaa ccaacatct 
      ccaaagtcc tcaaaagag tagcagcat ccagcattt 
      ctgccgatt ccatgtctc tgagttcac caccaggca 
      cacaaagtc cccaaggat tcgataagc tatcagatg 
      gacacgttc caaaggagg tctccagcc tctccgaac 
      aactccaaa atttgataa tgggccgag tgtctcaag 
      ccaaaactg cgtccagaa cgcgcaaaa ttgtcatct 
      atgtttgcg ccgcagact cgcaagatt gttacgtgg 
      aaaagggcg tcctccaca acagtgttt acccagaac 
      tgagggctc actgccggt cgaaaggtt acgaactac 
      acattgtgg acctacact ggaagaccc tcttggaga 
      aacaacaag accacgaat tccccgagc tttgcattg 
      gagagctta caaggcaaa tggaagtcg cctacatca 
      ttccggtac ttttcctga acaattctc tcggaacac 
      ctctcttgc ccataacca tcgaaagcg ccgatttta 
      agatggcaa tggaaagtg ccgaaaacc agtcagctc 
      agaccctcc tctccaagt ggtaccaga tcgacagtg 
      atgctcaac ccccttgct accgcctcc agccaattt 
      cctaccaca tcccgggct tcaaggaaa catccccag 
      aagagcgcg aacgcgctt tcgaagaat ggcgcaccg 
      aaatcgata aaatgttgg cggtactta tcaccgtct 
      tcacgcaag aactgcgcg acacttatc tcacgacag 
      tggtggagc aagaggtgc acaacacgg tgttcatga 
      gccaagagt tggccaaac gctgcattt ggatcaacc 
      gcgtctaca cacccacgg ccaaaaccc cggacaatt 
      tgagtccgg gggatgcag agctctacc ggaggctca 
      acacgctac agaaagatc tgagaaacc agttggccg 
      aaaaacata ttgttagga ttccggtga agtatgtgg 
      aaggtggtt tgaatccgg agattccgg aacacgcgc 
      aatacatag ctcaagtga ctgctcact tacaaaaac 
      acctagtgg aaatgatgg actcgatta tcgaagaac 
      atcagggga aaacaatgc tgaagccga gtcagggga 
      tcgaagcgc cgcttttcg aagaactga accaacaaa 
      cgagctttt gccaaaagg ccgcacaat gtagtatta 
      atagagaga aaacgatta acgaaatta aaacgtata 
      taatgggag ataacccag ttcctctaa tattatacg 
      gccccggtg gttgtggca agactactc tattggccc 
      gagtagcgc aatgttgcc agcagtggc tgccggaag 
      ccttcctaa tttttagat tcatcggta tcagtgccc 
      aatcaagca caatagagc aattactaa gttcgataa 
      cgaatcagt gttcgattt taacgtatg gacataaat 
      gttactgta tacataata tagaaactt atgaaaaaa 
      tccttccaa atctcttag cagctagtt gtctccagc 
      gccctttga tactaatcc tcgacggca tagaccaag 
      tccgctctt acagttcca aatcgatcg agtggcttc 
      ccaccaaac tccccgaca acatcaagc tggtgctct 
      ctgtatcag agggcagtg acgagttcc tgcaaatcg 
      gcaggaaaa tcagcccga acaccttca tcaagatgc 
      cgcttctgg gcgaagccg aggcgaaga acatcctca 
      tgtccagtg tgatgcaat acaatcata gcgttaatt 
      ctaaaatac aagactgcg ttctcaagt cggttcagg 
      agtgcacgc tgccgctct actccaagg tgctcgcct 
      ggcagactt cctggtggg cggacaagg agcacgaca 
      ttgtcccga aaggacacg ttaacgatc agatcagtc 
      tcatgctgg aggagctcg agacgattt tgggcgtca 
      cgcaagtcc agcacgctc ttgcaataa ttacaagca 
      ccaagcatg gggtcacag attcggaga tgatcgatt 
      tgttggcgt ttgacgaca gttttcaca gcacaacca 
      cttacgttc cttgggcgc cggcttgtt tgacttggt 
      cgagactca acaagcacc tggcgccct ttttgcaat 
      ggacgttga ctggggggg ttttggggg tgcaatgga 
      gggacaata tgctgagac aagcggtgg cagtgcggt 
      acaaagaac acgccaaat gggcaaacc aaatgattt 
      acgattatt acacgggca aatggtggg ccagcaagc 
      cagcgaacg tcctggcac gtctcgtgt cacaggaga 
      cgattctgg gaaaatgtt ataacaggc ggaagctgg 
      acgagctgc cgttccact attatcagt tgaacgggg 
      agatcgaga actcgccgt acatttcgg acattacgt 
      ggatttacg acaaagtct gcggctcga actgctacc 
      aaatcctcg aggacatta atttgcaga aaagtgtga 
      agaacgagc tgactttaa tgttgcgag attttatag 
      aaacccacg ccagtgtcc tcaactacg acggtcgac 
      agttctatt cgcacttgt acaaatact tagaagaca 
      aagttaggc gtaaggaga ttagtttag agtcgaatt 
      ccaccctag cccaggtgt acaacgtgg cgaaaaacc 
      cgccagttt tgtcactta tcccgctaa acgctggac 
      aggggaaag gcgatgcct ctgcccccc ttttccagt 
      acttcgatt tggtcacga gactaccgg aaacggacc 
      aatttgtca taagtgtgt ccacaagca aggaggaaa 
      taagcgttt gggacgtga aaaagtgcg ctcaagtgc 
      gcgttttga aaggcattc cgcatccga ccaacctga 
      tcccgattg accaataca ggtgcatag ttctttgtc 
      ggagggagt tgaggatat acaacctgg actgtggta 
      ccttcatga ccaaactga agggcgtca tgaaccaga 
      aaatgcctt acttcgggc ttcatgacc aaagccact 
      tggtggcac ttagcagaa accggatgt atgttaatc 
      ttatgaacc tggagactg gcgattgtg tcaccacat 
      tcaaggctg gggaggacc gctttctca attccctgc 
      tggtttcgg gggatggga gagtccttg tgtgtggcg 
      acgagacac aaaagccgt ttccgctcc tagtgtgga 
      acctccagt ccagaaaac tattatatg accttagaa 
      tacctcatc acgatttta taacttcac tgtcggcta 
      tcacgtacg aaggtagtt acgtttgct gtgtttgtc 
      acgaaatag acgaaccca atccgaatt tcatcgttg 
      tgtatgatt tgcaaagtg gtactttat ttaaaaaat 
      ggaaaccgt catgtaaca cggtttcct tagaaatta 
      gctcacaag gcggctgtg tcatatccg gtctcgagg 
      acgctcgaa tactcgttt gggacctta ttactggta 
      actgtcgat ggtctttat gtggccaca ccgctcccg 
      tttcctccc tcaagcttg atccaacgg ggggtttgt 
      gtttgtcga cggattcgg aatgcagag atagatcca 
      tccggcttt gggatctca ataaaggca acttggtgt 
      cggtttata caccaacca ggaacattg cagcgtgtg 
      aactgacgt ccaacggcc actacatcg tcctggccc 
      ttgaaggcg aggaagaag tggtaattt tgaagctaa 
      caggactcc gtatggacc taaacgagg aaaacgaaa 
      cgtacggag tggcggaga acactggca aaacttttg 
      aactgaagg agagtgatg cgtgctgac cgaaatagt 
      ttaactctt ttcaattat cacaggaat tgtttaaat 
      gtaattaag aattctccg agtcgtgct tatttgtca 
      ttgtttttt gggcaaaaa atacacaca tttcgatgg 
      actgtgctg gtagcagat ccagactac ttctttttt 
      tattatgag ttgtaatag tttattgga ttgttcctg 
      tccttgtac atgtacctt caatttcta tcaactttg 
      cttaattta cttcacgga acggactga tcagaaatt 
      gtcaaaagg aaatcaaga gtcactcca ctgccaaaa 
      gaaaatcac gacggttgc caaagtatt tgtaacgct 
      aagacttcg actttcgca aaaacgttg acaaagaag 
      ccggctaac caatgttat agagtattt taagcaaag 
      tcctattgt tgcaaagat atattccca tctggtatg 
      cggaacaat ataattaaa atattatat ttgtatgtt 
      gtgattctt gtgtaaaaa taaatgtaa taaacgatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY