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Gene information
General information
TC033520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1129216..1131620
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033520
    iBeetle screen iB_10241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033520 RA
      MIKILLQIA LLIPTCFSS NPCCRNNNA ISERRCIDG 
      SPLQGLSCP QKYLIEKDS DIEVDSDDH LNIASSRIS 
      PNKYCVSSK NDTVVYIVC FNEESTGNT IYIVHAVLE 
      LISVFFIVI TIFVYLQLR DDLLDLQGK SILHSIAGL 
      ALSFLTLAV NQLLPGGLE RPYCIVLAY IIYFSMLYS 
      FFWLNVLSF HIWRVIVKP RFLQILTPY WHYIYCGFG 
      CGGPTFLTI ILSIAQSMN ESDSHPGFG ETKCWFKST 
      KTQFLYFYT PMAVLLCLN LLYYGSTIA TLWRSLKTV 
      DEKKDKVLK YRLLLCVKL FFIMGISWI FELLSAAFQ 
      ESNPLQVIW HVTDVLNTL QGILIFLIL VVFRKRVVR 
      GLADRSFCG IRLPGRWRG AADDECGEI EEELNLSET 
      HVQKN
    CDS sequence
    • >TC033520 RA
      atgataaaa atattgtta caaatagca cttctaatt 
      ccgacctgt ttctcatcg aacccttgc tgcagaaac 
      aataatgcg ataagtgaa cgaagatgc atcgatggt 
      tcgcctttg caaggtctc tcctgtccc caaaagtac 
      ctcattgag aaagattct gacatagaa gtcgatagc 
      gatgatcat cttaacata gcctcgtca cgaatatca 
      ccaaacaaa tactgcgtg tcaagtaaa aacgacaca 
      gttgtttac atcgtttgc ttcaatgag gagtccaca 
      ggcaacaca atctacatc gttcatgct gttctagaa 
      ttaatttcc gtgtttttc attgtgata acgattttt 
      gtttattta caattgcga gacgacctt ctggatttg 
      caaggaaaa agcattctg cactcaatt gcaggactg 
      gctttatca ttccttact ttggccgta aaccaacta 
      ttgccaggt ggtttagaa cgaccatac tgtattgtg 
      ttggcctat attatctac ttctcaatg ctatattca 
      tttttttgg ctaaatgtt ttgtccttt cacatttgg 
      agagttatc gtaaagccc cgttttctc caaatattg 
      accccttat tggcactac atttactgt ggttttggt 
      tgtggagga cccactttt ttgaccatc atcctctca 
      attgctcag tcaatgaac gagtcagat tcccatcca 
      gggtttgga gaaacaaaa tgctggttt aaatccaca 
      aaaacccaa tttctttac ttttacaca cccatggcc 
      gtgctattg tgcctgaac ctcctctac tacggctca 
      acaatcgca actttgtgg cggagcttg aagacagtg 
      gatgaaaag aaagacaaa gtgttaaag tacagattg 
      cttctttgt gtaaaattg ttcttcata atgggcatc 
      tcttggatc tttgagtta ttgtcagcc gctttccaa 
      gagagcaat ccccttcaa gtcatatgg cacgtgaca 
      gacgttctc aacacctta caaggaatc ctgatattc 
      ctcattctg gtggtattc aggaaacga gtcgttcgt 
      ggattagca gatcggagt ttttgtgga atacgattg 
      ccagggcgg tggcgaggt gccgccgat gacgaatgt 
      ggagaaatc gaagaagaa ttgaactta tcagaaaca 
      cacgtccag aaaaactaa
    mRNA sequence
    • >TC033520 RA
      tgtttgttg gtgctccgc tagaaattc ggtgttttc 
      aaatgtaac gagatatgc gtggcgtgt ctctcacaa 
      aaaacgttt taaatcatg ctaattaat cgtatagtg 
      cgcactgaa agactcaaa atgccataa aaagttggt 
      tttacgctt gatttgcaa gtagtttta aacttacct 
      tcactgctt cacaaaaaa ttaatatgc atcagctaa 
      caatttgga aaatccaac aaaattaac aaccttaac 
      aaaaatagt aaacacaaa cccgcccaa aagttatcc 
      agactgtgt aaaaaatac gccaacaaa taacgacaa 
      tattttgaa gaaatcaac caaacaaaa aaggaaata 
      tttaatcag ctattttta atgtttatc cctcatttt 
      tgtgtcatt gatatttcc agcattaca agttcaacc 
      aattttttt actaaaatt tcgatatct aaagttgtt 
      ataattgtt ctaggtaaa cagccattc attgtcaca 
      ccttgttac aatctaatc tcagaaata aacttgtga 
      tccgttttg taatacgag ccaattaaa acatcgtga 
      catttgaca cgctaatta ttaaattga agttatttt 
      aatttacaa acatacaaa ttaagaagt gattaattg 
      caatgacga cactttgtt tacaatgat aaaaatatt 
      gttacaaat agcacttct aattccgac ctgtttctc 
      atcgaaccc ttgctgcag aaacaataa tgcgataag 
      tgaacgaag atgcatcga tggttcgcc tttgcaagg 
      tctctcctg tccccaaaa gtacctcat tgagaaaga 
      ttctgacat agaagtcga tagcgatga tcatcttaa 
      catagcctc gtcacgaat atcaccaaa caaatactg 
      cgtgtcaag taaaaacga cacagttgt ttacatcgt 
      ttgcttcaa tgaggagtc cacaggcaa cacaatcta 
      catcgttca tgctgttct agaattaat ttccgtgtt 
      tttcattgt gataacgat ttttgttta tttacaatt 
      gcgagacga ccttctgga tttgcaagg aaaaagcat 
      tctgcactc aattgcagg actggcttt atcattcct 
      tactttggc cgtaaacca actattgcc aggtggttt 
      agaacgacc atactgtat tgtgttggc ctatattat 
      ctacttctc aatgctata ttcattttt ttggctaaa 
      tgttttgtc ctttcacat ttggagagt tatcgtaaa 
      gccccgttt tctccaaat attgacccc ttattggca 
      ctacattta ctgtggttt tggttgtgg aggacccac 
      ttttttgac catcatcct ctcaattgc tcagtcaat 
      gaacgagtc agattccca tccagggtt tggagaaac 
      aaaatgctg gtttaaatc cacaaaaac ccaatttct 
      ttactttta cacacccat ggccgtgct attgtgcct 
      gaacctcct ctactacgg ctcaacaat cgcaacttt 
      gtggcggag cttgaagac agtggatga aaagaaaga 
      caaagtgtt aaagtacag attgcttct ttgtgtaaa 
      attgttctt cataatggg catctcttg gatctttga 
      gttattgtc agccgcttt ccaagagag caatcccct 
      tcaagtcat atggcacgt gacagacgt tctcaacac 
      cttacaagg aatcctgat attcctcat tctggtggt 
      attcaggaa acgagtcgt tcgtggatt agcagatcg 
      gagtttttg tggaatacg attgccagg gcggtggcg 
      aggtgccgc cgatgacga atgtggaga aatcgaaga 
      agaattgaa cttatcaga aacacacgt ccagaaaaa 
      ctaatcgcg tgtgttact ccatcagtg gaagagttt 
      agtgaaggt ctttgggcc aaggaacaa gacaaaatg 
      tacataaga caacgaaaa ttaaacata aactatttt 
      tagctaatt ttattttta cgcaaaact gggaaccga 
      tttacaaat tttcgtaat cacagtaga cttctattc 
      gcaatgaag atgcaactt tgaagactg gatgagatc 
      gagtgaaat atttctcat accgaatta tcctttaat 
      aaaattttt gttccccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY