Status
Please wait ...
Gene information
General information
TC031517
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004645,TC004644
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 4324295..4413415
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031517
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10237

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031517 RA
      MWTFLCYFT VLAAWIFAF EKRIFDVTN LPVQSIIIK 
      AGENKSLAC PGVNEHSLV IALEWLSLT HNVKLVEFM 
      SDSTTVWVN QHRIALLPD TFGLSFHPA IAEDSGDYV 
      CLVNSRPKP DGIVRLIVQ DVPDAPGRP LIVSFTSRS 
      VNLSWAPSQ DTHHSPITH YIIHTRVGE NGEWNVIDE 
      ILTPNNESS YQVTSLHPY TVYSFRVVA VNAMGASQP 
      SKESYYMVT LREAPAGKP TITTAHNTS STALHISWR 
      PPHHETIHG EFLGYRIAY RPRDRGDEA FKEIYIRDP 
      NVESHTIQN LETYTQYLV SLQVFNPEG PGPNTTVLV 
      MTDEGGTAV LLQIPCKSN SNHRLSPR
    CDS sequence
    • >TC031517 RA
      atgtggacg ttcctctgc tactttacg gtcctcgca 
      gcgtggatc ttcgccttc gaaaagcgc atcttcgat 
      gtaacgaac ctgcctgtc caaagtatc atcatcaaa 
      gcgggcgaa aacaaatct cttgcttgt cctggagta 
      aacgaacac tctttagtg atagcctta gaatggctc 
      tctctaact cacaacgtc aagctggtg gagttcatg 
      agtgattct acaacggtc tgggttaat caacatcgc 
      attgcactt cttcctgat actttcgga ttaagtttt 
      catcccgca attgccgag gacagtggt gactatgtt 
      tgtttagtt aatagcaga cccaagccg gacggcatc 
      gttcgtctc atagtgcaa gatgtgcct gatgcgccg 
      ggtcgccct ctcatcgtc agctttacg tctaggtcg 
      gtcaacctc tcgtgggcc ccctcgcaa gacacgcac 
      cacagtcca atcactcac tatatcatc cacactcgg 
      gttggcgag aacggcgaa tggaatgtg atagatgaa 
      attcttact ccgaacaac gagagcagt taccaagtt 
      acaagcttg cacccttac acagtgtac agtttcagg 
      gtggtagca gtgaatgcg atgggggcc agtcagcca 
      agcaaagag tcctattac atggttaca cttcgagag 
      gctcccgcg ggaaaaccg acgatcaca actgcgcat 
      aatacctcg tcgacagca ttacacatc agctggcga 
      ccgcctcat catgagacc atccacggc gaatttttg 
      ggctaccgt atagcatat cggccgagg gacagagga 
      gacgaagct ttcaaagag atatacatc cgggacccg 
      aatgtagag agccacact atacaaaat ttggagacc 
      tacacccag tatctcgtc tcgctccag gtgttcaac 
      cccgaagga cccgggccc aacaccacg gtcctggtc 
      atgaccgac gaaggaggt acggcagtc ttgctccaa 
      atcccgtgt aaaagcaac tctaatcac aggctttcg 
      ccacgataa
    mRNA sequence
    • >TC031517 RA
      tgtaatatt gggaatcgt cagtacatt tgcacacgc 
      gacccgatg cgacaagaa gcgcgctat tttaccctt 
      gattaatta atccgacac gagggatgt gttgttgtt 
      cccggtgtt tttgatgtg tgagtgcgg tcggagatg 
      aaatgagat gatggcatg tgcgacttc actgtggta 
      actcggtgc agtcggcac tatgtggac gttcctctg 
      ctactttac ggtcctcgc agcgtggat cttcgcctt 
      cgaaaagcg catcttcga tgtaacgaa cctgcctgt 
      ccaaagtat catcatcaa agcgggcga aaacaaatc 
      tcttgcttg tcctggagt aaacgaaca ctctttagt 
      gatagcctt agaatggct ctctctaac tcacaacgt 
      caagctggt ggagttcat gagtgattc tacaacggt 
      ctgggttaa tcaacatcg cattgcact tcttcctga 
      tactttcgg attaagttt tcatcccgc aattgccga 
      ggacagtgg tgactatgt ttgtttagt taatagcag 
      acccaagcc ggacggcat cgttcgtct catagtgca 
      agatgtgcc tgatgcgcc gggtcgccc tctcatcgt 
      cagctttac gtctaggtc ggtcaacct ctcgtgggc 
      cccctcgca agacacgca ccacagtcc aatcactca 
      ctatatcat ccacactcg ggttggcga gaacggcga 
      atggaatgt gatagatga aattcttac tccgaacaa 
      cgagagcag ttaccaagt tacaagctt gcaccctta 
      cacagtgta cagtttcag ggtggtagc agtgaatgc 
      gatgggggc cagtcagcc aagcaaaga gtcctatta 
      catggttac acttcgaga ggctcccgc gggaaaacc 
      gacgatcac aactgcgca taatacctc gtcgacagc 
      attacacat cagctggcg accgcctca tcatgagac 
      catccacgg cgaattttt gggctaccg tatagcata 
      tcggccgag ggacagagg agacgaagc tttcaaaga 
      gatatacat ccgggaccc gaatgtaga gagccacac 
      tatacaaaa tttggagac ctacaccca gtatctcgt 
      ctcgctcca ggtgttcaa ccccgaagg acccgggcc 
      caacaccac ggtcctggt catgaccga cgaaggagg 
      tacggcagt cttgctcca aatcccgtg taaaagcaa 
      ctctaatca caggctttc gccacgata agtctatgt 
      ttaagtttc acaattctt tcgtttcaa tgtgaacgg 
      tcagaagta atggaaaaa ttgcttctg cgccatctg 
      tcagtgtag gaacacact caaacgtgc tcccttttg 
      gccttaaga gtgttgttt ttcggtatg gtcatacag 
      agtcgctta ttcagctcg agtcggtaa tccccggtt 
      gccggactt agactaaag ctgcgggga tata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY