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Gene information
General information
TC014859 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9639338..9644552
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014859
    iBeetle screen iB_10234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014859 RA
      MSKAKIIEL DETNTCEFC CNALAKYTC PKCQSKYCT 
      LVCYQSEKH LQCSEEFYK DCIVKELAQ EDNEEAKKK 
      MLEILKRSH LEEEELDSD DDDFADIAD RLAGVDLDD 
      ADQVWEKLT KDEQQEFVA FLKSEDVTK LIPSWEPWW 
      SHKSPNVEE IESCQLYKS ECPQLIKIR DFREISKKN 
      PADCVKYNL INVLAAYAF ATRYFNGEH HDFAKEVIS 
      CVVTISLTL KNNQNFDDY ESAVKSVEY ESIMVVTFP 
      SYILYFY
    CDS sequence
    • >TC014859 RA
      atgtccaaa gctaaaata atcgaattg gacgagacg 
      aacacttgc gaattttgt tgcaatgct ttggccaag 
      tatacgtgc cccaaatgc cagtcaaaa tactgcaca 
      ttggtgtgc taccaatcg gagaagcat ttgcagtgt 
      tctgaggag ttttacaag gattgtata gttaaggaa 
      ttagcacag gaggacaat gaagaagcc aagaaaaaa 
      atgttggag attttgaaa cgaagtcat ttggaggaa 
      gaggagttg gattcggac gatgatgac tttgctgat 
      attgcagat agactggca ggggttgat ttggatgac 
      gctgatcaa gtatgggaa aaattgacc aaagatgag 
      caacaggaa tttgtcgca tttctaaag tccgaggat 
      gtgacaaaa ttaatccca tcgtgggaa ccttggtgg 
      tcccataaa tccccaaat gtggaagaa atcgagtct 
      tgccaactg tacaaatct gaatgtccc cagttgata 
      aaaattcgt gattttcga gaaatttcg aaaaaaaat 
      ccggccgat tgcgtcaaa tataatttg atcaacgtc 
      ctggcagcg tacgctttt gccactcgt tacttcaat 
      ggtgaacat cacgacttc gccaaggag gtgattagc 
      tgtgttgtt acaatttct ctcacgttg aaaaacaat 
      cagaatttc gacgactac gaaagcgcg gtcaaatcg 
      gtcgaatat gagtcgata atggtggta actttcccg 
      agttacata ctttatttt tactag
    mRNA sequence
    • >TC014859 RA
      ccttagttc cctccttac ataacctca accagtgtc 
      aaaaaatca aaatataaa tcatgagtc cggaagccc 
      cccctcgag acatctaca aagacaccc caatccgat 
      tattgggct acgcgaacg aagtcggtg agtctttga 
      ggggcttca ttggcacaa aatgggtca atgtctcgt 
      acggggtgg ccacattgt acgtagttg ctgacacaa 
      ttgacaaaa ctggtaaat catacattg tatgggagt 
      cagggagtt gggggctct ttcattgca atttttttt 
      tttcagaac aattcagga aagccaggt tgtgtcaag 
      aagactgtg ttcgcagct actgatact ctagtttgg 
      caaatgttg gcctcagtc gtcattcca ggatataca 
      attaatcgg gtgtgtgct ctgagtgat tttgtgttg 
      accaaaacg gccaaatta cccacaaat agtagaaaa 
      ttgcttgtt acaggagtt ggtctagca gtcattcct 
      tttatcatt aaacccata gataattta gtccattta 
      atgcttgac tctagtttg agaaaaata gccccctag 
      ttcagtagg tcaattgtt aattaattg ataaattta 
      aaaaaaact agtattatt tgttgcgac gtgtggtag 
      ccctgtcta gtgactaga aaaaatcag tgttgttat 
      tgttgctga ataaattaa aaagtgttg caaaattcg 
      tacaattgg tgatttcgg gtcaagcag aaatgtcca 
      aagctaaaa taatcgaat tggacgaga cgaacactt 
      gcgaatttt gttgcaatg ctttggcca agtatacgt 
      gccccaaat gccagtcaa aatactgca cattggtgt 
      gctaccaat cggagaagc atttgcagt gttctgagg 
      agttttaca aggattgta tagttaagg aattagcac 
      aggaggaca atgaagaag ccaagaaaa aaatgttgg 
      agattttga aacgaagtc atttggagg aagaggagt 
      tggattcgg acgatgatg actttgctg atattgcag 
      atagactgg caggggttg atttggatg acgctgatc 
      aagtatggg aaaaattga ccaaagatg agcaacagg 
      aatttgtcg catttctaa agtccgagg atgtgacaa 
      aattaatcc catcgtggg aaccttggt ggtcccata 
      aatccccaa atgtggaag aaatcgagt cttgccaac 
      tgtacaaat ctgaatgtc cccagttga taaaaattc 
      gtgattttc gagaaattt cgaaaaaaa atccggccg 
      attgcgtca aatataatt tgatcaacg tcctggcag 
      cgtacgctt ttgccactc gttacttca atggtgaac 
      atcacgact tcgccaagg aggtgatta gctgtgttg 
      ttacaattt ctctcacgt tgaaaaaca atcagaatt 
      tcgacgact acgaaagcg cggtcaaat cggtcgaat 
      atgagtcga taatggtgg taactttcc cgagttaca 
      tactttatt tttactaga ttttttaga gtgattgga 
      ttgtggccg atggcgaaa actttaaat tattacgag 
      acgatttgg ataaaattc tcgaaggtc caaatgcaa 
      cggaacgaa atttttacg tattgtgtg ccctttcgg 
      acttgcatc gactactca acacggcca gtgtgaaaa 
      aaacggaaa agaaatcat cgtttagta agaatttcc 
      cgaatgatt gtttcccag aggtgaaac tagaatcga 
      aaaataaaa ttaaaactt gtct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY