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Gene information
General information
TC030744 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8965772..8974129
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030744
    iBeetle screen iB_10232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 4
    egg chamber number decreased - Number of animals showing the phenotype: 4

    egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030744 RA
      MSEVRRKTP EITFDSEPP NDLDSSSGC ELSFELSSD 
      YVRSPSPRR SASSNPTYS PPYKRVRAL RLFDSPLTP 
      KTIIQKSAT TPAPRCRLF TEKPRSVTN TKQKPVANV 
      NPFTPNGFY QNKKRLRST SSLQIPKFD LNDSDVSDI 
      ENEPPAKRV PLQENNISR YHQEFLELE LIGSGQFGS 
      VYKCVNRLD GCIYAIKKS TKPVAGSVF EKTALNEVY 
      AHAVLGKHQ HVVRYYSAW AEDNHMVIQ NEYCNGGSL 
      ADRLQEGPL SPVELRQML MHVAEGLRY IHSEGLVHL 
      DIKPGNIFI SREKKIQFT NYDSADDGF EDLEESHYE 
      EELTYKIGD LGHVTSLSN PQVEEGDCR YLPNEILHE 
      DYSHLTKAD IFALGLTVI ESAGAGPLP KNGPDWHKL 
      REGEVPKLS QNLSRDMIN LIGLMIHPD PTMRPGASQ 
      VLHHPALCP VGKKTRAQL HRELNAEKL KNEILAKQL 
      KEAAICIKS IAPDSKQLK SVTRISNRL IGKKVNRSS 
      STTTF
    CDS sequence
    • >TC030744 RA
      atgtctgaa gtgcgccgg aagactccc gagatcacg 
      tttgactcg gagcccccc aacgatctg gactcgtcg 
      tcaggttgt gagctgtct ttcgaactt tcgagtgac 
      tatgtacgc agcccctca ccgaggcgt tcagcttcg 
      tccaacccg acatacagc cccccgtac aagcgggtg 
      cgagcgctg cgactgttc gacagccct ctcaccccc 
      aagactatc atacagaag tcggccacg acgccggcc 
      cccaggtgt cggctcttc acggagaag ccgcgcagt 
      gtcaccaac accaagcag aagccggtg gccaacgtc 
      aaccccttc acaccaaac ggcttttat cagaataag 
      aagcgcttg aggtcgacg agttcgtta caaatcccc 
      aagtttgat ttaaacgat tcagacgtg tcagacatc 
      gagaacgag cccccagct aaacgagtg ccgctccaa 
      gagaacaat atctcacgt tatcaccag gagttcctc 
      gagctcgaa ctgatcggt tcgggtcag ttcggttcg 
      gtttacaag tgtgtaaac cgtttggat ggttgcatt 
      tatgcgata aaaaaatca acgaaaccc gtagctgga 
      agtgtcttt gagaaaacg gctttgaac gaggtctac 
      gcccacgct gtcttgggc aaacaccag cacgtggtg 
      cgctactac tcggcctgg gccgaagac aaccacatg 
      gtgatccag aacgagtac tgcaatggg ggctcgttg 
      gccgaccga ctacaagag ggccccctc agcccggtg 
      gagctgcgc cagatgctg atgcacgtg gctgagggg 
      ctccgctac atccattcc gaaggcctc gtccacctt 
      gacatcaaa cccgggaac attttcata tcaagagag 
      aagaaaatc caattcacg aattacgac tccgccgat 
      gatggtttc gaagacttg gaggagagc cactacgag 
      gaggagctg acgtacaaa atcggcgat ttgggacac 
      gtgaccagc ttgagtaac ccccaggtg gaagaaggc 
      gactgtcgt tatctcccg aatgaaata cttcatgaa 
      gattacagt catttgacc aaagctgat attttcgcg 
      ctgggtttg acggtgatt gagagtgcg ggggcgggg 
      ccactgccg aaaaacggc cccgattgg cataaacta 
      cgcgaaggg gaggtgcca aagctgtct cagaatttg 
      agtagggat atgatcaat ttgattggt ttgatgata 
      catcccgac ccgactatg aggcccggg gcttcccag 
      gttttgcac cacccggcc ctttgcccc gttgggaag 
      aaaacaagg gctcagtta cacagggaa ttgaatgct 
      gaaaaattg aagaatgag attttggcg aagcagttg 
      aaggaagca gcgatttgt atcaagtcg atagccccc 
      gatagcaaa cagttgaag agcgtgacg aggattagt 
      aataggttg atcgggaag aaggtgaat aggagcagc 
      agcactact acgttctag
    mRNA sequence
    • >TC030744 RA
      cgattttcg taaccgttt ccgtgtcga aaatagagc 
      gtgtgtttg tttttttgc gaattttgg gcgtttttt 
      gtgcgtgag aaatgtctg aagtgcgcc ggaagactc 
      ccgagatca cgtttgact cggagcccc ccaacgatc 
      tggactcgt cgtcaggtt gtgagctgt ctttcgaac 
      tttcgagtg actatgtac gcagcccct caccgaggc 
      gttcagctt cgtccaacc cgacataca gccccccgt 
      acaagcggg tgcgagcgc tgcgactgt tcgacagcc 
      ctctcaccc ccaagacta tcatacaga agtcggcca 
      cgacgccgg cccccaggt gtcggctct tcacggaga 
      agccgcgca gtgtcacca acaccaagc agaagccgg 
      tggccaacg tcaacccct tcacaccaa acggctttt 
      atcagaata agaagcgct tgaggtcga cgagttcgt 
      tacaaatcc ccaagtttg atttaaacg attcagacg 
      tgtcagaca tcgagaacg agcccccag ctaaacgag 
      tgccgctcc aagagaaca atatctcac gttatcacc 
      aggagttcc tcgagctcg aactgatcg gttcgggtc 
      agttcggtt cggtttaca agtgtgtaa accgtttgg 
      atggttgca tttatgcga taaaaaaat caacgaaac 
      ccgtagctg gaagtgtct ttgagaaaa cggctttga 
      acgaggtct acgcccacg ctgtcttgg gcaaacacc 
      agcacgtgg tgcgctact actcggcct gggccgaag 
      acaaccaca tggtgatcc agaacgagt actgcaatg 
      ggggctcgt tggccgacc gactacaag agggccccc 
      tcagcccgg tggagctgc gccagatgc tgatgcacg 
      tggctgagg ggctccgct acatccatt ccgaaggcc 
      tcgtccacc ttgacatca aacccggga acattttca 
      tatcaagag agaagaaaa tccaattca cgaattacg 
      actccgccg atgatggtt tcgaagact tggaggaga 
      gccactacg aggaggagc tgacgtaca aaatcggcg 
      atttgggac acgtgacca gcttgagta acccccagg 
      tggaagaag gcgactgtc gttatctcc cgaatgaaa 
      tacttcatg aagattaca gtcatttga ccaaagctg 
      atattttcg cgctgggtt tgacggtga ttgagagtg 
      cgggggcgg ggccactgc cgaaaaacg gccccgatt 
      ggcataaac tacgcgaag gggaggtgc caaagctgt 
      ctcagaatt tgagtaggg atatgatca atttgattg 
      gtttgatga tacatcccg acccgacta tgaggcccg 
      gggcttccc aggttttgc accacccgg ccctttgcc 
      ccgttggga agaaaacaa gggctcagt tacacaggg 
      aattgaatg ctgaaaaat tgaagaatg agattttgg 
      cgaagcagt tgaaggaag cagcgattt gtatcaagt 
      cgatagccc ccgatagca aacagttga agagcgtga 
      cgaggatta gtaataggt tgatcggga agaaggtga 
      ataggagca gcagcacta ctacgttct agggattta 
      tttattacc aacactagt gatctagtt tcgtttcgc 
      acgaaatgg aatttaggc gttagacgt gtagactga 
      tattgatag caaggctgt aaccgtttt aattaaatt 
      gttaatttt tcgagcttg tttgtcgtt tgtacacta 
      atcaaaatt cctccaggt actaggttt catactttt 
      ttattttac atattttat acttatagc ttagattta 
      ttaaaggta tttgttata tttatgtga taattattt 
      ttaaatgat agagtaggt atagatgaa cagtgtaac 
      tctacattc ctttaactt gtgcctttg tcgcatcaa 
      tttattgca gcgatttgt tagtgcatt ctcttgatt 
      tgtaagaaa ttataccaa ataaacgat tggtcgctt 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY