Status
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Gene information
General information
TC014899 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 8909676..8911240
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014899
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10231

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014899 RA
      MSLTFTCNI CVKFVASVA PVCGVGCNG TTYLTKLQA 
      VVPEVEWSP NYRICRSCE SELDAAYEF RERSIRLDF 
      ERQVMGPPE TTRPIYPCY TCGELFVDG QQLSEHVDG 
      HHPAVEDVE IISNQAQIW SEHMYAKQR IGGEDIACE 
      NCPQKFMTR KELEKHVPI CAELKLKVG SCQVCGATG 
      ETTNDVVEH CINVHNLDK QEIKPFLCD KCPKRFARW 
      YLLQGHQRQ HSEKRDFVC MHCGRAFKT KIAVVHHEE 
      GHVSVRNYK CELCNKTFK TKLSLCAHV RVVHFGREK 
      DFACHLCKK RLLTKHSLG RHIRRHKGE KTFNCHLCS 
      SSFFSQPEL TNHVKRHAF LKEINPFKC QHCDKSYKL 
      NVSYKLHLR RNHGIGDAK LPEKKHGCG VCAKKFHTK 
      THLKEHVRR HVGTNRHQC EVCGNKYSD KGALTSHRR 
      NKHETEPPG INLL
    CDS sequence
    • >TC014899 RA
      atgagtctc actttcacc tgcaacatt tgcgtaaaa 
      ttcgttgcc agcgtagcc cctgtctgc ggtgttggc 
      tgcaacggt acaacctat ctcacgaaa ctgcaggct 
      gtggtcccc gaagtggag tggtcgccg aactaccgt 
      atatgtcgg tcgtgtgag tccgaattg gacgcagct 
      tacgagttc cgggaacgt agcattcgg cttgatttc 
      gagcgccag gttatgggc ccccctgag acaacacgt 
      ccaatttac ccgtgttac acttgcggt gaattattc 
      gtggatggc caacagttg tccgaacac gttgatggc 
      caccatcct gcagtcgaa gatgttgaa attatttca 
      aatcaagct cagatttgg tccgagcat atgtacgcc 
      aaacagcgc attggaggc gaagatatc gcttgcgag 
      aattgccct cagaaattc atgactcgg aaagaactg 
      gaaaaacat gttccaatt tgtgccgaa ttgaagctt 
      aaagtgggg tcctgtcaa gtgtgtggt gcaactggc 
      gagacgacg aacgatgtt gtcgagcat tgtattaac 
      gtgcacaac ctcgataag caggaaatc aaaccgttc 
      ctgtgcgat aaatgcccc aaacgtttc gcgcgctgg 
      tatctttta caaggacac caaagacag cattcggaa 
      aaaagagac ttcgtttgt atgcattgt gggcgcgct 
      tttaaaacg aaaatcgcg gtcgtacac cacgaagaa 
      ggtcacgtg agtgtacga aactacaaa tgcgagttg 
      tgtaataaa acctttaaa acaaaattg agtctctgc 
      gctcacgtg agagtggtc catttcggc cgtgagaag 
      gatttcgcg tgtcatttg tgcaagaag cgactttta 
      acgaaacat tctcttggc agacacatc aggagacac 
      aagggcgaa aaaactttc aactgtcac ctgtgcagc 
      agctcgttt ttttcacaa cccgagctc acaaatcat 
      gtcaaacgg cacgctttc ctcaaggaa attaatccg 
      tttaagtgc caacactgt gataaatct tacaaatta 
      aacgtttcg tataaattg catctaagg cgcaatcat 
      ggcataggc gacgccaaa ttaccggaa aaaaagcac 
      ggatgtgga gtttgtgcc aaaaaattc cacactaaa 
      acccacctt aaagagcac gtccgtaga catgtgggg 
      actaatcgg caccagtgc gaagtctgc ggcaacaaa 
      tactccgac aagggggca cttacttcc cataggagg 
      aacaagcat gaaacggaa ccgcccggc attaatctg 
      ttataa
    mRNA sequence
    • >TC014899 RA
      cagtttgaa atagttgtg tctttaaac cagtcggtg 
      ttgcttttt ttgtacatt attacaaga aaatcgaaa 
      ttggggaat aattttgtg cgctaggtg gcgcggcgg 
      tttgacatg agtaagtga ggttatgtc gttacagga 
      gccccaaaa tgagtctca ctttcacct gcaacattt 
      gcgtaaaat tcgttgcca gcgtagccc ctgtctgcg 
      gtgttggct gcaacggta caacctatc tcacgaaac 
      tgcaggctg tggtccccg aagtggagt ggtcgccga 
      actaccgta tatgtcggt cgtgtgagt ccgaattgg 
      acgcagctt acgagttcc gggaacgta gcattcggc 
      ttgatttcg agcgccagg ttatgggcc cccctgaga 
      caacacgtc caatttacc cgtgttaca cttgcggtg 
      aattattcg tggatggcc aacagttgt ccgaacacg 
      ttgatggcc accatcctg cagtcgaag atgttgaaa 
      ttatttcaa atcaagctc agatttggt ccgagcata 
      tgtacgcca aacagcgca ttggaggcg aagatatcg 
      cttgcgaga attgccctc agaaattca tgactcgga 
      aagaactgg aaaaacatg ttccaattt gtgccgaat 
      tgaagctta aagtggggt cctgtcaag tgtgtggtg 
      caactggcg agacgacga acgatgttg tcgagcatt 
      gtattaacg tgcacaacc tcgataagc aggaaatca 
      aaccgttcc tgtgcgata aatgcccca aacgtttcg 
      cgcgctggt atcttttac aaggacacc aaagacagc 
      attcggaaa aaagagact tcgtttgta tgcattgtg 
      ggcgcgctt ttaaaacga aaatcgcgg tcgtacacc 
      acgaagaag gtcacgtga gtgtacgaa actacaaat 
      gcgagttgt gtaataaaa cctttaaaa caaaattga 
      gtctctgcg ctcacgtga gagtggtcc atttcggcc 
      gtgagaagg atttcgcgt gtcatttgt gcaagaagc 
      gacttttaa cgaaacatt ctcttggca gacacatca 
      ggagacaca agggcgaaa aaactttca actgtcacc 
      tgtgcagca gctcgtttt tttcacaac ccgagctca 
      caaatcatg tcaaacggc acgctttcc tcaaggaaa 
      ttaatccgt ttaagtgcc aacactgtg ataaatctt 
      acaaattaa acgtttcgt ataaattgc atctaaggc 
      gcaatcatg gcataggcg acgccaaat taccggaaa 
      aaaagcacg gatgtggag tttgtgcca aaaaattcc 
      acactaaaa cccacctta aagagcacg tccgtagac 
      atgtgggga ctaatcggc accagtgcg aagtctgcg 
      gcaacaaat actccgaca agggggcac ttacttccc 
      ataggagga acaagcatg aaacggaac cgcccggca 
      ttaatctgt tataatacc gaagaaaat atactgttg 
      ttttgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY