Status
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Gene information
General information
TC015016 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 5043869..5056466
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015016
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015016 RA
      MFRFLSGRK NRTNTRTNG ASEIKPTKL ISKNLILCR 
      VILLDGTDL SVELSKKAE ARDLYEQVF YSLDLIEKD 
      YFGLQYTDV NHVKHWLDP TKSIKKQIK IGPPYTLRL 
      KVKFYSSEP NNLREELTR YQFFLQLKQ DILEGKLEC 
      PHQTDVELA ALALQSELG DYDESQHSA ATVSEFRFV 
      PNQTEEMEI EILEEFKKL RGLTPAQAE LSYLNKAKW 
      LEMYGVDMH TVLGKDGME YRLGLTPTG ILVFEGSQK 
      IGLFFWPKI GKLNFKKKK LTLVVVEDD DQGREQEHT 
      FVFRLHNEK ACKHLWKCA VEHHAFFRL RAPVKGPSA 
      RQNFFRMGS RFRYSGRTE YQSTHHNKP RRSVQLERR 
      PSQRYGRRQ SHVLREKQK QEQANETTE KTAENNEPS 
      SSRCSVRSN GPESFGKES VGQNSFGKG SIGCNSYSP 
      VLSTGSKNG TLKRNSTPT SPTTPSNDI QLDFLLKSL 
      AKETFSGMQ RDEARNEIN NKDVKDDEP KNVAKPLPP 
      GQLKCNVLK AKLEEGTKL TNQMFVAAV NNDEANLNS 
      LNAATFVSV GGDKLTLSV TGPASNPIL NNDDKKRCA 
      TPVTVTFFG HNDSGKLEK VMLNNHEKQ PLSLLSTDS 
      QVSDSESTA STNNSNPFA TYLTDNSTN PFQGLNPFL 
      TNNPFRNED EKDEEKSEV DESIKSVDE DNNVKTPTT 
      VTPPLSKLS PWLVSQEVV SSPVSKVNT TETTIIRKS 
      VITTQL
    CDS sequence
    • >TC015016 RA
      atgttccgc ttcctcagc ggccgaaaa aatcggaca 
      aacacccgc acaaacgga gcctccgaa ataaaaccg 
      acgaaacta ataagcaag aatttgatt ttgtgccgt 
      gttatcctc ctggatggc accgacctg tcggtggag 
      ttatcgaaa aaggccgaa gccagggat ctctacgag 
      caggtcttc tactccctg gacctgatc gagaaggac 
      tacttcggg ctgcagtac acggacgtg aaccatgtt 
      aagcattgg ctggacccc accaagtcc atcaaaaaa 
      caaatcaaa attgggccc ccttatact ttgagattg 
      aaagtcaag ttttactcc tctgagccg aacaattta 
      agggaggag ttgacgcgg taccagttc ttcctgcag 
      cttaagcag gatatttta gaggggaag ttggagtgc 
      ccccatcag actgatgtt gaactggcg gccttagcg 
      ctacagtcg gagttgggc gactacgat gaatcccaa 
      cactctgca gcaaccgtt tcggaattc cgtttcgtt 
      ccgaaccaa acggaagaa atggaaata gaaattttg 
      gaagaattc aaaaaactt cgcggttta actccagct 
      caagccgaa ctgagctac ttaaacaaa gccaaatgg 
      ctcgaaatg tacggtgtc gacatgcac accgtttta 
      ggaaaagac ggaatggaa taccgcctt ggtttaaca 
      ccaaccggt attttagta ttcgaaggc agccaaaaa 
      atcggtctc ttcttctgg cccaaaatc ggaaaactc 
      aacttcaag aaaaagaaa ctaactctc gtggtcgtc 
      gaagacgac gaccaaggt cgggaacaa gaacacact 
      ttcgttttt cgtttgcac aacgaaaag gcgtgtaaa 
      cacctatgg aagtgtgct gttgaacac cacgcattt 
      ttccggtta cgagcaccg gttaaggga ccttcagct 
      cgacagaat ttcttcaga atggggtcg aggtttcgg 
      tattcggga cgaaccgaa taccagagc acacatcat 
      aacaagccg agacgaagc gtccaattg gagcgaaga 
      ccgagtcag aggtacgga cggagacag agccatgtg 
      ttgagagag aaacagaag caggaacag gccaatgag 
      acgacggaa aaaaccgcc gaaaataac gaaccgagt 
      tcgagtagg tgcagtgtg aggtccaat gggccagag 
      tcctttggg aaggagtca gtggggcaa aattccttc 
      gggaagggg tccataggg tgcaattcc tacagtcct 
      gttttgtct actggtagt aaaaatggg accttgaag 
      agaaattcg acaccgaca agtccaacg actccaagt 
      aacgacatt cagcttgat tttctgctg aaaagtttg 
      gcgaaagaa acgttcagt ggaatgcaa cgagatgag 
      gcgaggaat gaaattaat aataaagat gtgaaggat 
      gacgaaccg aagaatgtg gcgaagcct ttgcctcca 
      ggacaactc aagtgtaac gtgctcaaa gcaaaactg 
      gaagaagga acgaaactg acaaatcaa atgtttgtg 
      gcagctgtg aacaacgac gaggcgaat ttaaattcc 
      ttgaacgcc gcaaccttc gtatcggtg ggaggcgat 
      aaattaaca ttatcggta acaggtccc gccagtaac 
      cctatccta aacaacgac gacaaaaaa cgatgcgca 
      acccctgta actgtaaca tttttcggc cataacgac 
      agcggtaaa ttagaaaaa gtcatgtta aacaatcac 
      gaaaaacag ccattgagt ctattaagc accgattcg 
      caagttagt gatagcgaa tcgaccgct tccacaaac 
      aattccaat cctttcgcg acttacttg accgacaac 
      agcaccaat cctttccaa ggtcttaat cctttcctt 
      actaacaat cccttcagg aacgaggac gagaaagac 
      gaggaaaaa tccgaagtg gatgagtcg attaagagc 
      gtcgatgag gataataat gtgaaaaca ccaacaact 
      gtgacaccg cctttgagt aaattatca ccgtggctt 
      gtgtcccag gaggtcgtg tcttcaccc gtttctaaa 
      gtaaacacc actgagacg acaattatc cgaaaatcg 
      gtcatcaca acacagtta taa
    mRNA sequence
    • >TC015016 RA
      ctctctctc cacttgttg tgtgtcatt tcgtcaagt 
      tggtggtag catggcggc tgtttggtg tgtgtgtgt 
      tggagttaa aacccgtaa ttatttatt aaatagcca 
      ttagtttga gcaaatgtt gcttaaaag ttgagtttg 
      gtcgttcca agtacaaaa atccagtga gaaatgttc 
      cgcttcctc agcggccga aaaaatcgg acaaacacc 
      cgcacaaac ggagcctcc gaaataaaa ccgacgaaa 
      ctaataagc aagaatttg attttgtgc cgtgttatc 
      ctcctggat ggcaccgac ctgtcggtg gagttatcg 
      aaaaaggcc gaagccagg gatctctac gagcaggtc 
      ttctactcc ctggacctg atcgagaag gactacttc 
      gggctgcag tacacggac gtgaaccat gttaagcat 
      tggctggac cccaccaag tccatcaaa aaacaaatc 
      aaaattggg cccccttat actttgaga ttgaaagtc 
      aagttttac tcctctgag ccgaacaat ttaagggag 
      gagttgacg cggtaccag ttcttcctg cagcttaag 
      caggatatt ttagagggg aagttggag tgcccccat 
      cagactgat gttgaactg gcggcctta gcgctacag 
      tcggagttg ggcgactac gatgaatcc caacactct 
      gcagcaacc gtttcggaa ttccgtttc gttccgaac 
      caaacggaa gaaatggaa atagaaatt ttggaagaa 
      ttcaaaaaa cttcgcggt ttaactcca gctcaagcc 
      gaactgagc tacttaaac aaagccaaa tggctcgaa 
      atgtacggt gtcgacatg cacaccgtt ttaggaaaa 
      gacggaatg gaataccgc cttggttta acaccaacc 
      ggtatttta gtattcgaa ggcagccaa aaaatcggt 
      ctcttcttc tggcccaaa atcggaaaa ctcaacttc 
      aagaaaaag aaactaact ctcgtggtc gtcgaagac 
      gacgaccaa ggtcgggaa caagaacac actttcgtt 
      tttcgtttg cacaacgaa aaggcgtgt aaacaccta 
      tggaagtgt gctgttgaa caccacgca tttttccgg 
      ttacgagca ccggttaag ggaccttca gctcgacag 
      aatttcttc agaatgggg tcgaggttt cggtattcg 
      ggacgaacc gaataccag agcacacat cataacaag 
      ccgagacga agcgtccaa ttggagcga agaccgagt 
      cagaggtac ggacggaga cagagccat gtgttgaga 
      gagaaacag aagcaggaa caggccaat gagacgacg 
      gaaaaaacc gccgaaaat aacgaaccg agttcgagt 
      aggtgcagt gtgaggtcc aatgggcca gagtccttt 
      gggaaggag tcagtgggg caaaattcc ttcgggaag 
      gggtccata gggtgcaat tcctacagt cctgttttg 
      tctactggt agtaaaaat gggaccttg aagagaaat 
      tcgacaccg acaagtcca acgactcca agtaacgac 
      attcagctt gattttctg ctgaaaagt ttggcgaaa 
      gaaacgttc agtggaatg caacgagat gaggcgagg 
      aatgaaatt aataataaa gatgtgaag gatgacgaa 
      ccgaagaat gtggcgaag cctttgcct ccaggacaa 
      ctcaagtgt aacgtgctc aaagcaaaa ctggaagaa 
      ggaacgaaa ctgacaaat caaatgttt gtggcagct 
      gtgaacaac gacgaggcg aatttaaat tccttgaac 
      gccgcaacc ttcgtatcg gtgggaggc gataaatta 
      acattatcg gtaacaggt cccgccagt aaccctatc 
      ctaaacaac gacgacaaa aaacgatgc gcaacccct 
      gtaactgta acatttttc ggccataac gacagcggt 
      aaattagaa aaagtcatg ttaaacaat cacgaaaaa 
      cagccattg agtctatta agcaccgat tcgcaagtt 
      agtgatagc gaatcgacc gcttccaca aacaattcc 
      aatcctttc gcgacttac ttgaccgac aacagcacc 
      aatcctttc caaggtctt aatcctttc cttactaac 
      aatcccttc aggaacgag gacgagaaa gacgaggaa 
      aaatccgaa gtggatgag tcgattaag agcgtcgat 
      gaggataat aatgtgaaa acaccaaca actgtgaca 
      ccgcctttg agtaaatta tcaccgtgg cttgtgtcc 
      caggaggtc gtgtcttca cccgtttct aaagtaaac 
      accactgag acgacaatt atccgaaaa tcggtcatc 
      acaacacag ttataaaca aattggtgc tttgttttc 
      tctacaatg tgataaaat ctcctctcg caatatttt 
      aagtactta atgtttttt attttgtat atttttgta 
      ggtttaaat ttttttatc attcacttc catttctta 
      gactgtgcc tttcagtat ttcatcaat acttccatt 
      actaacaac acgaggacc aattgtgtg atttttaaa 
      acggtaatt gtaaagtgc cattatttt atgaaagtt 
      tgtattcaa ttttatgaa ccgatccgt cctttcgat 
      gaaattata gattcttta tgatacaaa taaatattt 
      atataatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY