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Gene information
General information
TC015693 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4815616..4820112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015693
    iBeetle screen iB_10211

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015693 RA
      MAFSVLRSV VKCTRSVCA LKPPKNVAN ICRCATPLA 
      ATIRLHSNL QAPAGVDHA QKIKSKNIY SSSESDSDL 
      EPPGHRGET QFWRRKIRT FHGILDVNK DGVISYDDF 
      KLLAERFIE LGHLSEKHT HEFRNLLKD LWEKRWGPI 
      NPYNLVTVE QFLEDMHHV INDKHLVRR AHSFLPYLF 
      RALDKDQSG EISEDEFKL FFQCLGLQE EDAILAFRS 
      MDEDGDGVV TMKEFVKHG REFLITEDE NKISKYFFG 
      PLMEH
    CDS sequence
    • >TC015693 RA
      atggcgttc tccgtgcta agaagtgtt gtaaagtgc 
      accaggagt gtgtgtgcc ctaaagccg cccaaaaat 
      gtggctaat atttgccga tgtgccacc cccttagcc 
      gccaccatc cgcctccac tcgaacctc caagcgccc 
      gcaggggtg gaccatgcc caaaaaatc aaaagtaaa 
      aacatatac agctcatcg gaatccgac agcgattta 
      gaaccgcca ggacaccga ggagaaaca caattctgg 
      cgccgcaaa atccgcact ttccacgga atcctcgac 
      gtgaacaaa gacggcgtc atctcctac gacgatttc 
      aaactactc gctgaaaga ttcatcgaa ctgggccac 
      ttgagcgag aaacacact cacgagttc cgcaatttg 
      cttaaagac ttgtgggag aagcgctgg gggcccatc 
      aacccctac aacctcgtc accgtcgag cagttcctc 
      gaggacatg caccacgtg atcaacgat aagcacctg 
      gtgcggagg gcgcactcc ttcctgccc tatctcttc 
      cgcgccctt gataaagac cagtcgggc gagatctcc 
      gaagacgag tttaaatta tttttccaa tgtttgggt 
      cttcaggag gaggatgcc attttggct tttcgcagc 
      atggacgaa gatggcgac ggagtggtg acgatgaag 
      gagtttgtc aaacatgga cgcgagttt ttaattacg 
      gaagatgag aataaaatc agcaaatac tttttcggg 
      cctttgatg gagcactga
    mRNA sequence
    • >TC015693 RA
      cataaataa taatgtttt gaccaaatc ttcgacaat 
      attgccttc gactcggta ccaaacacc acttccacg 
      ctcccattg gtcgcatga gggtaccca taaaagaaa 
      ataaaagaa gtgatagga aggacagag acagtcgtc 
      tcgatgtaa aaaagccaa cttgttgat gatacggcc 
      catagtttg acccgaacc agaaccaaa ttgacttcc 
      agataacta acagtgatg gcgttctcc gtgctaaga 
      agtgttgta aagtgcacc aggagtgtg tgtgcccta 
      aagccgccc aaaaatgtg gctaatatt tgccgatgt 
      gccaccccc ttagccgcc accatccgc ctccactcg 
      aacctccaa gcgcccgca ggggtggac catgcccaa 
      aaaatcaaa agtaaaaac atatacagc tcatcggaa 
      tccgacagc gatttagaa ccgccagga caccgagga 
      gaaacacaa ttctggcgc cgcaaaatc cgcactttc 
      cacggaatc ctcgacgtg aacaaagac ggcgtcatc 
      tcctacgac gatttcaaa ctactcgct gaaagattc 
      atcgaactg ggccacttg agcgagaaa cacactcac 
      gagttccgc aatttgctt aaagacttg tgggagaag 
      cgctggggg cccatcaac ccctacaac ctcgtcacc 
      gtcgagcag ttcctcgag gacatgcac cacgtgatc 
      aacgataag cacctggtg cggagggcg cactccttc 
      ctgccctat ctcttccgc gcccttgat aaagaccag 
      tcgggcgag atctccgaa gacgagttt aaattattt 
      ttccaatgt ttgggtctt caggaggag gatgccatt 
      ttggctttt cgcagcatg gacgaagat ggcgacgga 
      gtggtgacg atgaaggag tttgtcaaa catggacgc 
      gagttttta attacggaa gatgagaat aaaatcagc 
      aaatacttt ttcgggcct ttgatggag cactgattt 
      gaaacgaga caagtggcg cgatctcga gacgatgct 
      cggaacggg agagttgac aagggggcc gccagaggg 
      ccgaaatca atattttaa aacttatta ggaatgtaa 
      caataaata cgtaataaa acaattttt tattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY