Status
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Gene information
General information
TC015660 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4301852..4304421
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015660
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015660 RA
      MAQSKLNDL AGKFGKGGP PGLGLGLKL LAVGGAAAY 
      GISQAMYTV EGGHRAIMF NRIGGVQKD IYTEGLHFR 
      VPWFQYPII YDIRSRPRK ISSPTGSKD LQMVNISLR 
      VLSRPNASQ LPIVYRQLG LDYDEKVLP SICNEVLKS 
      VVAKFNAAQ LITQRQQVS LLVRRELTE RARDFNIIL 
      DDVSITELS FGKEYTAAV EAKQVAQQE AQRAAFIVE 
      KAKQERQQK IVQAEGEAE AAKMLGEAI SKNPGYLKL 
      RKIRAAQNI SRTIANSQN KVYLSGNSL MLNISDKEF 
      DDQSDKLKR NEYDDTTRS ADKNVLRTA RKMATRIAG
    • >TC015660 RB
      MAQSKLNDL AGKFGKGGP PGLGLGLKL LAVGGAAAY 
      GISQAMYTV EGGHRAIMF NRIGGVQKD IYTEGLHFR 
      VPWFQYPII YDIRSRPRK ISSPTGSKD LQMVNISLR 
      VLSRPNASQ LPIVYRQLG LDYDEKVLP SICNEVLKS 
      VVAKFNAAQ LITQRQQVS LLVRRELTE RARDFNIIL 
      DDVSITELS FGKEYTAAV EAKQVAQQE AQRAAFIVE 
      KAKQERQQK IVQAEGEAE AAKMLGEAI SKNPGYLKL 
      RKIRAAQNI SRTIANSQN KVYLSGNSL MLNISDKEF 
      DDQSDKLKR KD
    CDS sequence
    • >TC015660 RA
      atggctcaa agcaagttg aacgacctg gccggcaag 
      ttcggcaaa gggggcccg cccggcctg gggctcgga 
      ctcaaactt ctggccgtt ggtggcgcc gcagcttac 
      ggtatctcg caagccatg tacaccgtc gaagggggc 
      caccgggcc atcatgttc aaccgcatt gggggcgtc 
      cagaaggac atttacact gaggggctg catttccgc 
      gtgccgtgg ttccagtac cccatcatt tacgacatt 
      cggtcaagg ccgcgtaag atctcctcg ccaacgggg 
      tcgaaagac ttgcagatg gtgaatatt tcgctgagg 
      gtgctctca aggccgaat gccagccaa ctgccgatt 
      gtttataga cagttgggg ctggattat gatgagaag 
      gttttgcct tcgatttgc aacgaggtg ctgaagtcg 
      gttgttgcg aagtttaac gcagctcag ttgattaca 
      caaagacag caagtttcg cttttggtt aggagggaa 
      ttgactgaa agggcgcgc gattttaat ataattctg 
      gacgatgtt tccattact gagctttcc ttcgggaag 
      gaatacacg gctgcggtt gaagccaaa caggtggcg 
      caacaggaa gcccaaagg gctgcgttt attgtcgag 
      aaagccaag caggaaagg cagcaaaag attgtccaa 
      gcggagggc gaagccgaa gccgcaaaa atgttaggt 
      gaagcaatt tccaaaaat ccgggctat ttgaaattg 
      aggaaaatc cgcgcggct cagaatatt tcacgaacc 
      attgccaac tcgcaaaat aaagtgtac ctcagtggt 
      aacagtcta atgttgaat atctctgat aaggagttt 
      gatgaccag tctgataag ctcaaacgt aatgaatat 
      gatgacacg acgcgaagt gctgataaa aatgtgctc 
      aggacagca cggaaaatg gcaacaagg attgctgga 
      taa
    • >TC015660 RB
      atggctcaa agcaagttg aacgacctg gccggcaag 
      ttcggcaaa gggggcccg cccggcctg gggctcgga 
      ctcaaactt ctggccgtt ggtggcgcc gcagcttac 
      ggtatctcg caagccatg tacaccgtc gaagggggc 
      caccgggcc atcatgttc aaccgcatt gggggcgtc 
      cagaaggac atttacact gaggggctg catttccgc 
      gtgccgtgg ttccagtac cccatcatt tacgacatt 
      cggtcaagg ccgcgtaag atctcctcg ccaacgggg 
      tcgaaagac ttgcagatg gtgaatatt tcgctgagg 
      gtgctctca aggccgaat gccagccaa ctgccgatt 
      gtttataga cagttgggg ctggattat gatgagaag 
      gttttgcct tcgatttgc aacgaggtg ctgaagtcg 
      gttgttgcg aagtttaac gcagctcag ttgattaca 
      caaagacag caagtttcg cttttggtt aggagggaa 
      ttgactgaa agggcgcgc gattttaat ataattctg 
      gacgatgtt tccattact gagctttcc ttcgggaag 
      gaatacacg gctgcggtt gaagccaaa caggtggcg 
      caacaggaa gcccaaagg gctgcgttt attgtcgag 
      aaagccaag caggaaagg cagcaaaag attgtccaa 
      gcggagggc gaagccgaa gccgcaaaa atgttaggt 
      gaagcaatt tccaaaaat ccgggctat ttgaaattg 
      aggaaaatc cgcgcggct cagaatatt tcacgaacc 
      attgccaac tcgcaaaat aaagtgtac ctcagtggt 
      aacagtcta atgttgaat atctctgat aaggagttt 
      gatgaccag tctgataag ctcaaacgt aaggattga
    mRNA sequence
    • >TC015660 RA
      gtcaccggc gccattttc cgcaatcac gttttttca 
      atcgcgcgt tgtgtaact aatagcccc cataaatgg 
      ctcaaagca agttgaacg acctggccg gcaagttcg 
      gcaaagggg gcccgcccg gcctggggc tcggactca 
      aacttctgg ccgttggtg gcgccgcag cttacggta 
      tctcgcaag ccatgtaca ccgtcgaag ggggccacc 
      gggccatca tgttcaacc gcattgggg gcgtccaga 
      aggacattt acactgagg ggctgcatt tccgcgtgc 
      cgtggttcc agtacccca tcatttacg acattcggt 
      caaggccgc gtaagatct cctcgccaa cggggtcga 
      aagacttgc agatggtga atatttcgc tgagggtgc 
      tctcaaggc cgaatgcca gccaactgc cgattgttt 
      atagacagt tggggctgg attatgatg agaaggttt 
      tgccttcga tttgcaacg aggtgctga agtcggttg 
      ttgcgaagt ttaacgcag ctcagttga ttacacaaa 
      gacagcaag tttcgcttt tggttagga gggaattga 
      ctgaaaggg cgcgcgatt ttaatataa ttctggacg 
      atgtttcca ttactgagc tttccttcg ggaaggaat 
      acacggctg cggttgaag ccaaacagg tggcgcaac 
      aggaagccc aaagggctg cgtttattg tcgagaaag 
      ccaagcagg aaaggcagc aaaagattg tccaagcgg 
      agggcgaag ccgaagccg caaaaatgt taggtgaag 
      caatttcca aaaatccgg gctatttga aattgagga 
      aaatccgcg cggctcaga atatttcac gaaccattg 
      ccaactcgc aaaataaag tgtacctca gtggtaaca 
      gtctaatgt tgaatatct ctgataagg agtttgatg 
      accagtctg ataagctca aacgtaatg aatatgatg 
      acacgacgc gaagtgctg ataaaaatg tgctcagga 
      cagcacgga aaatggcaa caaggattg ctggataag 
      ggcggaatt ttcacgcat gtagatttt atattttgg 
      ttattgttg cttaataaa cgtttttaa cacct
    • >TC015660 RB
      gtcaccggc gccattttc cgcaatcac gttttttca 
      atcgcgcgt tgtgtaact aatagcccc cataaatgg 
      ctcaaagca agttgaacg acctggccg gcaagttcg 
      gcaaagggg gcccgcccg gcctggggc tcggactca 
      aacttctgg ccgttggtg gcgccgcag cttacggta 
      tctcgcaag ccatgtaca ccgtcgaag ggggccacc 
      gggccatca tgttcaacc gcattgggg gcgtccaga 
      aggacattt acactgagg ggctgcatt tccgcgtgc 
      cgtggttcc agtacccca tcatttacg acattcggt 
      caaggccgc gtaagatct cctcgccaa cggggtcga 
      aagacttgc agatggtga atatttcgc tgagggtgc 
      tctcaaggc cgaatgcca gccaactgc cgattgttt 
      atagacagt tggggctgg attatgatg agaaggttt 
      tgccttcga tttgcaacg aggtgctga agtcggttg 
      ttgcgaagt ttaacgcag ctcagttga ttacacaaa 
      gacagcaag tttcgcttt tggttagga gggaattga 
      ctgaaaggg cgcgcgatt ttaatataa ttctggacg 
      atgtttcca ttactgagc tttccttcg ggaaggaat 
      acacggctg cggttgaag ccaaacagg tggcgcaac 
      aggaagccc aaagggctg cgtttattg tcgagaaag 
      ccaagcagg aaaggcagc aaaagattg tccaagcgg 
      agggcgaag ccgaagccg caaaaatgt taggtgaag 
      caatttcca aaaatccgg gctatttga aattgagga 
      aaatccgcg cggctcaga atatttcac gaaccattg 
      ccaactcgc aaaataaag tgtacctca gtggtaaca 
      gtctaatgt tgaatatct ctgataagg agtttgatg 
      accagtctg ataagctca aacgtaagg attgagtct 
      aggtttaag ttcgtagtg agtgagtga aaaactttt 
      ataagaata tttctttgt tactatttg taaaaatat 
      tcctaagtc tcaaataaa tgcgtaagt tgttacgaa 
      gtcttgttt gattttgtt taccccttt tgtaggtaa 
      tgaatatga tgacacgac gcgaagtgc tgataaaaa 
      tgtgctcag gacagcacg gaaaatggc aacaaggat 
      tgctggata agggcggaa ttttcacgc atgtagatt 
      ttatatttt ggttattgt tgcttaata aacgttttt 
      aacacct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY