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Gene information
General information
TC015049 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4229059..4233562
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015049
    iBeetle screen iB_10206

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015049 RA
      MSRKRRHSP PRPLSSYAT ELAPFLDHH ANIRNPHDF 
      WKFHEKYTT LGKTDRSKI LNLQFTSND LYDKLPVLD 
      RKGARIRIS RDDFHHFLT SVRVYQDFQ QKSKFAKLR 
      KLRRGQSEL PIAGYRQEI LDKLGGCRV MLIAGDTGC 
      GKSTQVPQF VYQGGYKKI VCTQPRRIA CVSLAKRVA 
      HETLTDFKS TVGYQIRFE KSKRADTSI VFMTEGLLL 
      RQAQEEDTL NSYDVIILD EVHERHLHG DFLVGIMKC 
      LLYKRQDFK LILMSATIN LDLFTNYFK REKLEVVRV 
      PGRLFPIEI VYRPIIRDP YERKREKLD CTPYLQIIQ 
      MIDEKYQPS QKGDLLIFL NGYSEISTL ADAVSEYSQ 
      VKKNWIVLQ LHSSLSLEE QDKVFDYPP EGVRKCIIS 
      TNIAETSVT IDGIRFVID SGKVNRMTY HTSGGVNKL 
      TETTISQDS AKQRSGRAG RTGPGICYR LYSEEDFKN 
      FEIFTPAEI HLVPLDTLL LHMISLGLT DLNHFPFLE 
      KPSEKSIEE GVEKLKFTG ALELKINCL ALTPLGDAL 
      SQLPVDLSI GKMLVLSTV FGNVNAVLA VAALLSIQS 
      PLTQNAYRN TEAQDLRRV LDSNHGDPI SMLNYYKEW 
      LSVKQSSVP VKSRYEKGE SSKVWARKR CLEEQRFYE 
      ATKLLEQFR DILNEANLL SKVAAHGLS SEQRAMRHG 
      ELKQLKSLR YKLKNEKKS ERKQLKYEI FSGDKEEDE 
      TTDLRDVEF RITNDFTRL QKLLNEASA DSYKDLVLL 
      KLILTSGLY PQIAVEDEF NSSKTVSER LYHTKNKNY 
      VFLRPLSYF ATNPEVLEL HNDDIEVPP PGYFSKRPI 
      SKKHQLLIY QTILETKKV YLVNTMRMP AIQTLMLFS 
      KTIASNKTL TKFVFDDFL LLDVPFYGQ GKTLLMRAL 
      KLRHKWKHE LESKLKDPS RDFNKKEIF YFIDDLVNF 
      VNTDVNYNI KRLLPADLK EIYTGEPGG VGVNEKNPF 
      EESFKIMQN NELGGVQMT ENFTYGCLL QEEWACSIE 
      ERVETTPFE CQYCKETKL GLSVFKIIQ HEEFCRPEI 
      DKPEVETVV VKANSKVFR CDKCGKDLV LTPVEILKH 
      KKACN
    CDS sequence
    • >TC015049 RA
      atgtcgcgc aaacggcgc cactcgccc ccacgaccc 
      ctctcgtcc tacgccact gagttggcc cccttcctg 
      gaccaccac gccaacatc cgtaacccg cacgatttc 
      tggaaattc cacgaaaag tacaccaca ttgggtaaa 
      accgaccga agcaaaatc ctcaatttg caattcaca 
      tccaacgac ctctacgac aaactccca gtgctcgac 
      cggaagggg gcccgcatc cggatatca cgtgacgac 
      ttccaccat ttcctcacc tcagttcga gtgtaccaa 
      gacttccaa cagaagagc aaatttgcg aagttgcgc 
      aaactgaga cgggggcaa agcgagctc cccatcgcg 
      gggtaccgc caagagatt ttggacaag ttggggggc 
      tgtcgggtt atgttgatc gcgggggac accgggtgt 
      gggaaaagt acgcaagtg ccacagttt gtgtaccaa 
      gggggctat aaaaagatt gtgtgtacg caaccgagg 
      cggatcgcg tgtgtgtcg ttagcgaag cgagtggct 
      catgagact ctgacggac tttaagagc acggtgggg 
      taccagatt aggttcgag aagagcaag agagctgat 
      acttctata gtcttcatg accgaaggg ctattatta 
      agacaagcg caagaagaa gacactttg aattcatac 
      gacgttatt attttggac gaagtgcat gagaggcat 
      ctccatggg gatttccta gtcgggatc atgaagtgc 
      ctcctgtac aaaagacaa gactttaaa ttaatctta 
      atgtctgca acaataaat ctcgacttg tttacaaac 
      tacttcaag cgagagaag ctcgaagtt gtccgagtg 
      cccggtcgg ctatttccg atcgaaatc gtctataga 
      cctataatc cgagacccc tacgaacga aaaagggaa 
      aaacttgac tgtacccct tatttgcaa ataatccaa 
      atgattgat gagaaatac caaccgagc caaaaaggc 
      gacttgttg attttccta aatgggtat agtgaaata 
      tcaacacta gcagatgct gttagtgaa tatagccaa 
      gttaagaag aactggatt gtgctccaa ctccactca 
      agtctgagt cttgaggaa caggacaaa gtcttcgat 
      tatccacca gagggcgtc cgcaagtgc atcataagc 
      acgaacatt gccgaaact tcggtcaca atcgacgga 
      atccgattc gttatagac tcggggaaa gtcaaccgt 
      atgacatac cacacctct ggtggtgtt aacaaattg 
      accgaaaca acaatatca caagacagc gctaaacaa 
      cgttcggga agagccggc cggacaggc cctggcatt 
      tgctaccgc ctctactct gaggaagat ttcaaaaac 
      ttcgaaatt ttcactcct gccgaaatc catctcgta 
      cccttagac accctctta ttacatatg atttcgttg 
      gggttaacc gacttgaac cacttcccc ttcctcgaa 
      aaacccagc gagaaaagc atcgaagaa ggcgtggag 
      aaattgaaa ttcacaggc gccttagaa ctcaaaatc 
      aactgtctt gccttaacc ccgctaggg gacgctttg 
      agccaacta ccggtcgat ctaagcatc ggcaaaatg 
      ctagtcttg agcacagtt ttcggtaat gttaacgcc 
      gtccttgcg gtggcggca ctgctcagc atccagtca 
      cctctgacg caaaacgct taccgaaac actgaagcg 
      caagacttg cgccgagta ttggattcg aaccatggt 
      gacccgatc tcgatgctt aactactac aaagagtgg 
      ctgtcggta aaacagagc tctgtaccc gtgaaatca 
      cggtacgag aaaggcgag tcgtccaaa gtttgggcg 
      cggaagcgg tgtctcgag gaacaaagg ttttacgaa 
      gcgaccaag ctattggaa cagtttagg gatatcctg 
      aacgaagcg aatctccta tcaaaggtg gcggcgcac 
      ggattgtca agcgagcag cgtgctatg agacacggg 
      gagctcaaa cagttgaaa tcgctgaga tataagctc 
      aagaatgag aaaaagtcg gagaggaag caactcaag 
      tatgagatt tttagtggg gataaggag gaagatgaa 
      actacggat ttacgggat gtggagttt cgcataact 
      aatgatttt acccgactc cagaaacta ctaaatgag 
      gcttcggct gatagttac aaagatttg gttttattg 
      aaattgata ctaacaagt gggttgtac cctcaaatt 
      gcggttgag gatgagttt aattcgagt aaaactgtg 
      tcggaacgc ttgtaccac actaaaaac aaaaactac 
      gttttcttg cgtcctttg agttatttt gcaacgaat 
      ccggaggtt ctagagttg cataatgat gatatagag 
      gtgccccct cctggctac ttcagtaag cgaccaatc 
      agtaaaaaa caccaattg ttaatctac cagacgatc 
      cttgagaca aaaaaggtc tatcttgtt aataccatg 
      cgaatgcct gccatccaa actctgatg cttttcagc 
      aaaactata gccagtaac aagactttg acaaagttt 
      gtttttgac gatttcttg ctccttgat gtccctttt 
      tacggccag ggtaaaaca ctgttaatg cgagcgttg 
      aaactgaga cacaagtgg aaacacgaa cttgagtcc 
      aaattgaag gacccgtcg cgcgatttt aacaaaaaa 
      gagattttc tattttatc gacgatttg gtcaatttt 
      gtcaacact gatgttaat tataacata aaacggcta 
      ctaccggcc gatttgaag gaaatctac acgggggaa 
      ccgggaggg gtcggagtc aacgaaaaa aatcccttt 
      gaggagtct ttcaaaatt atgcaaaat aatgagtta 
      ggaggggtt caaatgact gaaaatttc acatacggg 
      tgtttgctg caagaagag tgggcctgt agtattgag 
      gaaagggtt gaaacgacc cctttcgaa tgccagtat 
      tgcaaggaa actaaattg gggctgtcg gtgtttaaa 
      ataatacag catgaggag ttctgtcgc ccggagata 
      gacaagcct gaggtggaa actgttgtt gttaaggcg 
      aattctaaa gtttttcgt tgtgataag tgcggaaaa 
      gacctggtt ttgaccccc gttgagatt ttgaagcac 
      aaaaaggcg tgtaattag
    mRNA sequence
    • >TC015049 RA
      gcccttatc taatcacca ctaagataa ttatcacta 
      ttacgtgat agagataat tttaatcag taaaatgac 
      caagctttg cgtcagacg tgaaccact tccagccct 
      aaccttaaa ctttcccac taacaatta cactgtttt 
      aagcccaaa aatgtactt aaaagtaca gttttacgc 
      gaaaatgtg tgataaggg cccataata ttgcgtcac 
      taatcctta tcattaaat ttatcacca ttacgtgaa 
      cctaaccca aaagttcag agtttacaa taaacaatg 
      tcgcgcaaa cggcgccac tcgccccca cgacccctc 
      tcgtcctac gccactgag ttggccccc ttcctggac 
      caccacgcc aacatccgt aacccgcac gatttctgg 
      aaattccac gaaaagtac accacattg ggtaaaacc 
      gaccgaagc aaaatcctc aatttgcaa ttcacatcc 
      aacgacctc tacgacaaa ctcccagtg ctcgaccgg 
      aagggggcc cgcatccgg atatcacgt gacgacttc 
      caccatttc ctcacctca gttcgagtg taccaagac 
      ttccaacag aagagcaaa tttgcgaag ttgcgcaaa 
      ctgagacgg gggcaaagc gagctcccc atcgcgggg 
      taccgccaa gagattttg gacaagttg gggggctgt 
      cgggttatg ttgatcgcg ggggacacc gggtgtggg 
      aaaagtacg caagtgcca cagtttgtg taccaaggg 
      ggctataaa aagattgtg tgtacgcaa ccgaggcgg 
      atcgcgtgt gtgtcgtta gcgaagcga gtggctcat 
      gagactctg acggacttt aagagcacg gtggggtac 
      cagattagg ttcgagaag agcaagaga gctgatact 
      tctatagtc ttcatgacc gaagggcta ttattaaga 
      caagcgcaa gaagaagac actttgaat tcatacgac 
      gttattatt ttggacgaa gtgcatgag aggcatctc 
      catggggat ttcctagtc gggatcatg aagtgcctc 
      ctgtacaaa agacaagac tttaaatta atcttaatg 
      tctgcaaca ataaatctc gacttgttt acaaactac 
      ttcaagcga gagaagctc gaagttgtc cgagtgccc 
      ggtcggcta tttccgatc gaaatcgtc tatagacct 
      ataatccga gacccctac gaacgaaaa agggaaaaa 
      cttgactgt accccttat ttgcaaata atccaaatg 
      attgatgag aaataccaa ccgagccaa aaaggcgac 
      ttgttgatt ttcctaaat gggtatagt gaaatatca 
      acactagca gatgctgtt agtgaatat agccaagtt 
      aagaagaac tggattgtg ctccaactc cactcaagt 
      ctgagtctt gaggaacag gacaaagtc ttcgattat 
      ccaccagag ggcgtccgc aagtgcatc ataagcacg 
      aacattgcc gaaacttcg gtcacaatc gacggaatc 
      cgattcgtt atagactcg gggaaagtc aaccgtatg 
      acataccac acctctggt ggtgttaac aaattgacc 
      gaaacaaca atatcacaa gacagcgct aaacaacgt 
      tcgggaaga gccggccgg acaggccct ggcatttgc 
      taccgcctc tactctgag gaagatttc aaaaacttc 
      gaaattttc actcctgcc gaaatccat ctcgtaccc 
      ttagacacc ctcttatta catatgatt tcgttgggg 
      ttaaccgac ttgaaccac ttccccttc ctcgaaaaa 
      cccagcgag aaaagcatc gaagaaggc gtggagaaa 
      ttgaaattc acaggcgcc ttagaactc aaaatcaac 
      tgtcttgcc ttaaccccg ctaggggac gctttgagc 
      caactaccg gtcgatcta agcatcggc aaaatgcta 
      gtcttgagc acagttttc ggtaatgtt aacgccgtc 
      cttgcggtg gcggcactg ctcagcatc cagtcacct 
      ctgacgcaa aacgcttac cgaaacact gaagcgcaa 
      gacttgcgc cgagtattg gattcgaac catggtgac 
      ccgatctcg atgcttaac tactacaaa gagtggctg 
      tcggtaaaa cagagctct gtacccgtg aaatcacgg 
      tacgagaaa ggcgagtcg tccaaagtt tgggcgcgg 
      aagcggtgt ctcgaggaa caaaggttt tacgaagcg 
      accaagcta ttggaacag tttagggat atcctgaac 
      gaagcgaat ctcctatca aaggtggcg gcgcacgga 
      ttgtcaagc gagcagcgt gctatgaga cacggggag 
      ctcaaacag ttgaaatcg ctgagatat aagctcaag 
      aatgagaaa aagtcggag aggaagcaa ctcaagtat 
      gagattttt agtggggat aaggaggaa gatgaaact 
      acggattta cgggatgtg gagtttcgc ataactaat 
      gattttacc cgactccag aaactacta aatgaggct 
      tcggctgat agttacaaa gatttggtt ttattgaaa 
      ttgatacta acaagtggg ttgtaccct caaattgcg 
      gttgaggat gagtttaat tcgagtaaa actgtgtcg 
      gaacgcttg taccacact aaaaacaaa aactacgtt 
      ttcttgcgt cctttgagt tattttgca acgaatccg 
      gaggttcta gagttgcat aatgatgat atagaggtg 
      ccccctcct ggctacttc agtaagcga ccaatcagt 
      aaaaaacac caattgtta atctaccag acgatcctt 
      gagacaaaa aaggtctat cttgttaat accatgcga 
      atgcctgcc atccaaact ctgatgctt ttcagcaaa 
      actatagcc agtaacaag actttgaca aagtttgtt 
      tttgacgat ttcttgctc cttgatgtc cctttttac 
      ggccagggt aaaacactg ttaatgcga gcgttgaaa 
      ctgagacac aagtggaaa cacgaactt gagtccaaa 
      ttgaaggac ccgtcgcgc gattttaac aaaaaagag 
      attttctat tttatcgac gatttggtc aattttgtc 
      aacactgat gttaattat aacataaaa cggctacta 
      ccggccgat ttgaaggaa atctacacg ggggaaccg 
      ggaggggtc ggagtcaac gaaaaaaat ccctttgag 
      gagtctttc aaaattatg caaaataat gagttagga 
      ggggttcaa atgactgaa aatttcaca tacgggtgt 
      ttgctgcaa gaagagtgg gcctgtagt attgaggaa 
      agggttgaa acgacccct ttcgaatgc cagtattgc 
      aaggaaact aaattgggg ctgtcggtg tttaaaata 
      atacagcat gaggagttc tgtcgcccg gagatagac 
      aagcctgag gtggaaact gttgttgtt aaggcgaat 
      tctaaagtt tttcgttgt gataagtgc ggaaaagac 
      ctggttttg acccccgtt gagattttg aagcacaaa 
      aaggcgtgt aattaggga gcggtgctt cttgtgttg 
      taaatattt cttgtttaa ataaataag atcaacaga 
      t
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