Status
Please wait ...
Gene information
General information
TC015049 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4229059..4233562
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015049
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10206

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015049 RA
      MSRKRRHSP PRPLSSYAT ELAPFLDHH ANIRNPHDF 
WKFHEKYTT LGKTDRSKI LNLQFTSND LYDKLPVLD 
RKGARIRIS RDDFHHFLT SVRVYQDFQ QKSKFAKLR 
KLRRGQSEL PIAGYRQEI LDKLGGCRV MLIAGDTGC 
GKSTQVPQF VYQGGYKKI VCTQPRRIA CVSLAKRVA 
HETLTDFKS TVGYQIRFE KSKRADTSI VFMTEGLLL 
RQAQEEDTL NSYDVIILD EVHERHLHG DFLVGIMKC 
LLYKRQDFK LILMSATIN LDLFTNYFK REKLEVVRV 
PGRLFPIEI VYRPIIRDP YERKREKLD CTPYLQIIQ 
MIDEKYQPS QKGDLLIFL NGYSEISTL ADAVSEYSQ 
VKKNWIVLQ LHSSLSLEE QDKVFDYPP EGVRKCIIS 
TNIAETSVT IDGIRFVID SGKVNRMTY HTSGGVNKL 
TETTISQDS AKQRSGRAG RTGPGICYR LYSEEDFKN 
FEIFTPAEI HLVPLDTLL LHMISLGLT DLNHFPFLE 
KPSEKSIEE GVEKLKFTG ALELKINCL ALTPLGDAL 
SQLPVDLSI GKMLVLSTV FGNVNAVLA VAALLSIQS 
PLTQNAYRN TEAQDLRRV LDSNHGDPI SMLNYYKEW 
LSVKQSSVP VKSRYEKGE SSKVWARKR CLEEQRFYE 
ATKLLEQFR DILNEANLL SKVAAHGLS SEQRAMRHG 
ELKQLKSLR YKLKNEKKS ERKQLKYEI FSGDKEEDE 
TTDLRDVEF RITNDFTRL QKLLNEASA DSYKDLVLL 
KLILTSGLY PQIAVEDEF NSSKTVSER LYHTKNKNY 
VFLRPLSYF ATNPEVLEL HNDDIEVPP PGYFSKRPI 
SKKHQLLIY QTILETKKV YLVNTMRMP AIQTLMLFS 
KTIASNKTL TKFVFDDFL LLDVPFYGQ GKTLLMRAL 
KLRHKWKHE LESKLKDPS RDFNKKEIF YFIDDLVNF 
VNTDVNYNI KRLLPADLK EIYTGEPGG VGVNEKNPF 
EESFKIMQN NELGGVQMT ENFTYGCLL QEEWACSIE 
ERVETTPFE CQYCKETKL GLSVFKIIQ HEEFCRPEI 
DKPEVETVV VKANSKVFR CDKCGKDLV LTPVEILKH 
KKACN
    CDS sequence
    • >TC015049 RA
      atgtcgcgc aaacggcgc cactcgccc ccacgaccc 
ctctcgtcc tacgccact gagttggcc cccttcctg 
gaccaccac gccaacatc cgtaacccg cacgatttc 
tggaaattc cacgaaaag tacaccaca ttgggtaaa 
accgaccga agcaaaatc ctcaatttg caattcaca 
tccaacgac ctctacgac aaactccca gtgctcgac 
cggaagggg gcccgcatc cggatatca cgtgacgac 
ttccaccat ttcctcacc tcagttcga gtgtaccaa 
gacttccaa cagaagagc aaatttgcg aagttgcgc 
aaactgaga cgggggcaa agcgagctc cccatcgcg 
gggtaccgc caagagatt ttggacaag ttggggggc 
tgtcgggtt atgttgatc gcgggggac accgggtgt 
gggaaaagt acgcaagtg ccacagttt gtgtaccaa 
gggggctat aaaaagatt gtgtgtacg caaccgagg 
cggatcgcg tgtgtgtcg ttagcgaag cgagtggct 
catgagact ctgacggac tttaagagc acggtgggg 
taccagatt aggttcgag aagagcaag agagctgat 
acttctata gtcttcatg accgaaggg ctattatta 
agacaagcg caagaagaa gacactttg aattcatac 
gacgttatt attttggac gaagtgcat gagaggcat 
ctccatggg gatttccta gtcgggatc atgaagtgc 
ctcctgtac aaaagacaa gactttaaa ttaatctta 
atgtctgca acaataaat ctcgacttg tttacaaac 
tacttcaag cgagagaag ctcgaagtt gtccgagtg 
cccggtcgg ctatttccg atcgaaatc gtctataga 
cctataatc cgagacccc tacgaacga aaaagggaa 
aaacttgac tgtacccct tatttgcaa ataatccaa 
atgattgat gagaaatac caaccgagc caaaaaggc 
gacttgttg attttccta aatgggtat agtgaaata 
tcaacacta gcagatgct gttagtgaa tatagccaa 
gttaagaag aactggatt gtgctccaa ctccactca 
agtctgagt cttgaggaa caggacaaa gtcttcgat 
tatccacca gagggcgtc cgcaagtgc atcataagc 
acgaacatt gccgaaact tcggtcaca atcgacgga 
atccgattc gttatagac tcggggaaa gtcaaccgt 
atgacatac cacacctct ggtggtgtt aacaaattg 
accgaaaca acaatatca caagacagc gctaaacaa 
cgttcggga agagccggc cggacaggc cctggcatt 
tgctaccgc ctctactct gaggaagat ttcaaaaac 
ttcgaaatt ttcactcct gccgaaatc catctcgta 
cccttagac accctctta ttacatatg atttcgttg 
gggttaacc gacttgaac cacttcccc ttcctcgaa 
aaacccagc gagaaaagc atcgaagaa ggcgtggag 
aaattgaaa ttcacaggc gccttagaa ctcaaaatc 
aactgtctt gccttaacc ccgctaggg gacgctttg 
agccaacta ccggtcgat ctaagcatc ggcaaaatg 
ctagtcttg agcacagtt ttcggtaat gttaacgcc 
gtccttgcg gtggcggca ctgctcagc atccagtca 
cctctgacg caaaacgct taccgaaac actgaagcg 
caagacttg cgccgagta ttggattcg aaccatggt 
gacccgatc tcgatgctt aactactac aaagagtgg 
ctgtcggta aaacagagc tctgtaccc gtgaaatca 
cggtacgag aaaggcgag tcgtccaaa gtttgggcg 
cggaagcgg tgtctcgag gaacaaagg ttttacgaa 
gcgaccaag ctattggaa cagtttagg gatatcctg 
aacgaagcg aatctccta tcaaaggtg gcggcgcac 
ggattgtca agcgagcag cgtgctatg agacacggg 
gagctcaaa cagttgaaa tcgctgaga tataagctc 
aagaatgag aaaaagtcg gagaggaag caactcaag 
tatgagatt tttagtggg gataaggag gaagatgaa 
actacggat ttacgggat gtggagttt cgcataact 
aatgatttt acccgactc cagaaacta ctaaatgag 
gcttcggct gatagttac aaagatttg gttttattg 
aaattgata ctaacaagt gggttgtac cctcaaatt 
gcggttgag gatgagttt aattcgagt aaaactgtg 
tcggaacgc ttgtaccac actaaaaac aaaaactac 
gttttcttg cgtcctttg agttatttt gcaacgaat 
ccggaggtt ctagagttg cataatgat gatatagag 
gtgccccct cctggctac ttcagtaag cgaccaatc 
agtaaaaaa caccaattg ttaatctac cagacgatc 
cttgagaca aaaaaggtc tatcttgtt aataccatg 
cgaatgcct gccatccaa actctgatg cttttcagc 
aaaactata gccagtaac aagactttg acaaagttt 
gtttttgac gatttcttg ctccttgat gtccctttt 
tacggccag ggtaaaaca ctgttaatg cgagcgttg 
aaactgaga cacaagtgg aaacacgaa cttgagtcc 
aaattgaag gacccgtcg cgcgatttt aacaaaaaa 
gagattttc tattttatc gacgatttg gtcaatttt 
gtcaacact gatgttaat tataacata aaacggcta 
ctaccggcc gatttgaag gaaatctac acgggggaa 
ccgggaggg gtcggagtc aacgaaaaa aatcccttt 
gaggagtct ttcaaaatt atgcaaaat aatgagtta 
ggaggggtt caaatgact gaaaatttc acatacggg 
tgtttgctg caagaagag tgggcctgt agtattgag 
gaaagggtt gaaacgacc cctttcgaa tgccagtat 
tgcaaggaa actaaattg gggctgtcg gtgtttaaa 
ataatacag catgaggag ttctgtcgc ccggagata 
gacaagcct gaggtggaa actgttgtt gttaaggcg 
aattctaaa gtttttcgt tgtgataag tgcggaaaa 
gacctggtt ttgaccccc gttgagatt ttgaagcac 
aaaaaggcg tgtaattag
    mRNA sequence
    • >TC015049 RA
      gcccttatc taatcacca ctaagataa ttatcacta 
ttacgtgat agagataat tttaatcag taaaatgac 
caagctttg cgtcagacg tgaaccact tccagccct 
aaccttaaa ctttcccac taacaatta cactgtttt 
aagcccaaa aatgtactt aaaagtaca gttttacgc 
gaaaatgtg tgataaggg cccataata ttgcgtcac 
taatcctta tcattaaat ttatcacca ttacgtgaa 
cctaaccca aaagttcag agtttacaa taaacaatg 
tcgcgcaaa cggcgccac tcgccccca cgacccctc 
tcgtcctac gccactgag ttggccccc ttcctggac 
caccacgcc aacatccgt aacccgcac gatttctgg 
aaattccac gaaaagtac accacattg ggtaaaacc 
gaccgaagc aaaatcctc aatttgcaa ttcacatcc 
aacgacctc tacgacaaa ctcccagtg ctcgaccgg 
aagggggcc cgcatccgg atatcacgt gacgacttc 
caccatttc ctcacctca gttcgagtg taccaagac 
ttccaacag aagagcaaa tttgcgaag ttgcgcaaa 
ctgagacgg gggcaaagc gagctcccc atcgcgggg 
taccgccaa gagattttg gacaagttg gggggctgt 
cgggttatg ttgatcgcg ggggacacc gggtgtggg 
aaaagtacg caagtgcca cagtttgtg taccaaggg 
ggctataaa aagattgtg tgtacgcaa ccgaggcgg 
atcgcgtgt gtgtcgtta gcgaagcga gtggctcat 
gagactctg acggacttt aagagcacg gtggggtac 
cagattagg ttcgagaag agcaagaga gctgatact 
tctatagtc ttcatgacc gaagggcta ttattaaga 
caagcgcaa gaagaagac actttgaat tcatacgac 
gttattatt ttggacgaa gtgcatgag aggcatctc 
catggggat ttcctagtc gggatcatg aagtgcctc 
ctgtacaaa agacaagac tttaaatta atcttaatg 
tctgcaaca ataaatctc gacttgttt acaaactac 
ttcaagcga gagaagctc gaagttgtc cgagtgccc 
ggtcggcta tttccgatc gaaatcgtc tatagacct 
ataatccga gacccctac gaacgaaaa agggaaaaa 
cttgactgt accccttat ttgcaaata atccaaatg 
attgatgag aaataccaa ccgagccaa aaaggcgac 
ttgttgatt ttcctaaat gggtatagt gaaatatca 
acactagca gatgctgtt agtgaatat agccaagtt 
aagaagaac tggattgtg ctccaactc cactcaagt 
ctgagtctt gaggaacag gacaaagtc ttcgattat 
ccaccagag ggcgtccgc aagtgcatc ataagcacg 
aacattgcc gaaacttcg gtcacaatc gacggaatc 
cgattcgtt atagactcg gggaaagtc aaccgtatg 
acataccac acctctggt ggtgttaac aaattgacc 
gaaacaaca atatcacaa gacagcgct aaacaacgt 
tcgggaaga gccggccgg acaggccct ggcatttgc 
taccgcctc tactctgag gaagatttc aaaaacttc 
gaaattttc actcctgcc gaaatccat ctcgtaccc 
ttagacacc ctcttatta catatgatt tcgttgggg 
ttaaccgac ttgaaccac ttccccttc ctcgaaaaa 
cccagcgag aaaagcatc gaagaaggc gtggagaaa 
ttgaaattc acaggcgcc ttagaactc aaaatcaac 
tgtcttgcc ttaaccccg ctaggggac gctttgagc 
caactaccg gtcgatcta agcatcggc aaaatgcta 
gtcttgagc acagttttc ggtaatgtt aacgccgtc 
cttgcggtg gcggcactg ctcagcatc cagtcacct 
ctgacgcaa aacgcttac cgaaacact gaagcgcaa 
gacttgcgc cgagtattg gattcgaac catggtgac 
ccgatctcg atgcttaac tactacaaa gagtggctg 
tcggtaaaa cagagctct gtacccgtg aaatcacgg 
tacgagaaa ggcgagtcg tccaaagtt tgggcgcgg 
aagcggtgt ctcgaggaa caaaggttt tacgaagcg 
accaagcta ttggaacag tttagggat atcctgaac 
gaagcgaat ctcctatca aaggtggcg gcgcacgga 
ttgtcaagc gagcagcgt gctatgaga cacggggag 
ctcaaacag ttgaaatcg ctgagatat aagctcaag 
aatgagaaa aagtcggag aggaagcaa ctcaagtat 
gagattttt agtggggat aaggaggaa gatgaaact 
acggattta cgggatgtg gagtttcgc ataactaat 
gattttacc cgactccag aaactacta aatgaggct 
tcggctgat agttacaaa gatttggtt ttattgaaa 
ttgatacta acaagtggg ttgtaccct caaattgcg 
gttgaggat gagtttaat tcgagtaaa actgtgtcg 
gaacgcttg taccacact aaaaacaaa aactacgtt 
ttcttgcgt cctttgagt tattttgca acgaatccg 
gaggttcta gagttgcat aatgatgat atagaggtg 
ccccctcct ggctacttc agtaagcga ccaatcagt 
aaaaaacac caattgtta atctaccag acgatcctt 
gagacaaaa aaggtctat cttgttaat accatgcga 
atgcctgcc atccaaact ctgatgctt ttcagcaaa 
actatagcc agtaacaag actttgaca aagtttgtt 
tttgacgat ttcttgctc cttgatgtc cctttttac 
ggccagggt aaaacactg ttaatgcga gcgttgaaa 
ctgagacac aagtggaaa cacgaactt gagtccaaa 
ttgaaggac ccgtcgcgc gattttaac aaaaaagag 
attttctat tttatcgac gatttggtc aattttgtc 
aacactgat gttaattat aacataaaa cggctacta 
ccggccgat ttgaaggaa atctacacg ggggaaccg 
ggaggggtc ggagtcaac gaaaaaaat ccctttgag 
gagtctttc aaaattatg caaaataat gagttagga 
ggggttcaa atgactgaa aatttcaca tacgggtgt 
ttgctgcaa gaagagtgg gcctgtagt attgaggaa 
agggttgaa acgacccct ttcgaatgc cagtattgc 
aaggaaact aaattgggg ctgtcggtg tttaaaata 
atacagcat gaggagttc tgtcgcccg gagatagac 
aagcctgag gtggaaact gttgttgtt aaggcgaat 
tctaaagtt tttcgttgt gataagtgc ggaaaagac 
ctggttttg acccccgtt gagattttg aagcacaaa 
aaggcgtgt aattaggga gcggtgctt cttgtgttg 
taaatattt cttgtttaa ataaataag atcaacaga 
t

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY