| General information | |||
| TC015049 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007421.3: 4229059..4233562 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC015049 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10206 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
abdominal odoriferous glands content darker - Number of animals showing the phenotype: 1 (13 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC015049 RA
MSRKRRHSP PRPLSSYAT ELAPFLDHH ANIRNPHDF WKFHEKYTT LGKTDRSKI LNLQFTSND LYDKLPVLD RKGARIRIS RDDFHHFLT SVRVYQDFQ QKSKFAKLR KLRRGQSEL PIAGYRQEI LDKLGGCRV MLIAGDTGC GKSTQVPQF VYQGGYKKI VCTQPRRIA CVSLAKRVA HETLTDFKS TVGYQIRFE KSKRADTSI VFMTEGLLL RQAQEEDTL NSYDVIILD EVHERHLHG DFLVGIMKC LLYKRQDFK LILMSATIN LDLFTNYFK REKLEVVRV PGRLFPIEI VYRPIIRDP YERKREKLD CTPYLQIIQ MIDEKYQPS QKGDLLIFL NGYSEISTL ADAVSEYSQ VKKNWIVLQ LHSSLSLEE QDKVFDYPP EGVRKCIIS TNIAETSVT IDGIRFVID SGKVNRMTY HTSGGVNKL TETTISQDS AKQRSGRAG RTGPGICYR LYSEEDFKN FEIFTPAEI HLVPLDTLL LHMISLGLT DLNHFPFLE KPSEKSIEE GVEKLKFTG ALELKINCL ALTPLGDAL SQLPVDLSI GKMLVLSTV FGNVNAVLA VAALLSIQS PLTQNAYRN TEAQDLRRV LDSNHGDPI SMLNYYKEW LSVKQSSVP VKSRYEKGE SSKVWARKR CLEEQRFYE ATKLLEQFR DILNEANLL SKVAAHGLS SEQRAMRHG ELKQLKSLR YKLKNEKKS ERKQLKYEI FSGDKEEDE TTDLRDVEF RITNDFTRL QKLLNEASA DSYKDLVLL KLILTSGLY PQIAVEDEF NSSKTVSER LYHTKNKNY VFLRPLSYF ATNPEVLEL HNDDIEVPP PGYFSKRPI SKKHQLLIY QTILETKKV YLVNTMRMP AIQTLMLFS KTIASNKTL TKFVFDDFL LLDVPFYGQ GKTLLMRAL KLRHKWKHE LESKLKDPS RDFNKKEIF YFIDDLVNF VNTDVNYNI KRLLPADLK EIYTGEPGG VGVNEKNPF EESFKIMQN NELGGVQMT ENFTYGCLL QEEWACSIE ERVETTPFE CQYCKETKL GLSVFKIIQ HEEFCRPEI DKPEVETVV VKANSKVFR CDKCGKDLV LTPVEILKH KKACN
- >TC015049 RA
atgtcgcgc aaacggcgc cactcgccc ccacgaccc ctctcgtcc tacgccact gagttggcc cccttcctg gaccaccac gccaacatc cgtaacccg cacgatttc tggaaattc cacgaaaag tacaccaca ttgggtaaa accgaccga agcaaaatc ctcaatttg caattcaca tccaacgac ctctacgac aaactccca gtgctcgac cggaagggg gcccgcatc cggatatca cgtgacgac ttccaccat ttcctcacc tcagttcga gtgtaccaa gacttccaa cagaagagc aaatttgcg aagttgcgc aaactgaga cgggggcaa agcgagctc cccatcgcg gggtaccgc caagagatt ttggacaag ttggggggc tgtcgggtt atgttgatc gcgggggac accgggtgt gggaaaagt acgcaagtg ccacagttt gtgtaccaa gggggctat aaaaagatt gtgtgtacg caaccgagg cggatcgcg tgtgtgtcg ttagcgaag cgagtggct catgagact ctgacggac tttaagagc acggtgggg taccagatt aggttcgag aagagcaag agagctgat acttctata gtcttcatg accgaaggg ctattatta agacaagcg caagaagaa gacactttg aattcatac gacgttatt attttggac gaagtgcat gagaggcat ctccatggg gatttccta gtcgggatc atgaagtgc ctcctgtac aaaagacaa gactttaaa ttaatctta atgtctgca acaataaat ctcgacttg tttacaaac tacttcaag cgagagaag ctcgaagtt gtccgagtg cccggtcgg ctatttccg atcgaaatc gtctataga cctataatc cgagacccc tacgaacga aaaagggaa aaacttgac tgtacccct tatttgcaa ataatccaa atgattgat gagaaatac caaccgagc caaaaaggc gacttgttg attttccta aatgggtat agtgaaata tcaacacta gcagatgct gttagtgaa tatagccaa gttaagaag aactggatt gtgctccaa ctccactca agtctgagt cttgaggaa caggacaaa gtcttcgat tatccacca gagggcgtc cgcaagtgc atcataagc acgaacatt gccgaaact tcggtcaca atcgacgga atccgattc gttatagac tcggggaaa gtcaaccgt atgacatac cacacctct ggtggtgtt aacaaattg accgaaaca acaatatca caagacagc gctaaacaa cgttcggga agagccggc cggacaggc cctggcatt tgctaccgc ctctactct gaggaagat ttcaaaaac ttcgaaatt ttcactcct gccgaaatc catctcgta cccttagac accctctta ttacatatg atttcgttg gggttaacc gacttgaac cacttcccc ttcctcgaa aaacccagc gagaaaagc atcgaagaa ggcgtggag aaattgaaa ttcacaggc gccttagaa ctcaaaatc aactgtctt gccttaacc ccgctaggg gacgctttg agccaacta ccggtcgat ctaagcatc ggcaaaatg ctagtcttg agcacagtt ttcggtaat gttaacgcc gtccttgcg gtggcggca ctgctcagc atccagtca cctctgacg caaaacgct taccgaaac actgaagcg caagacttg cgccgagta ttggattcg aaccatggt gacccgatc tcgatgctt aactactac aaagagtgg ctgtcggta aaacagagc tctgtaccc gtgaaatca cggtacgag aaaggcgag tcgtccaaa gtttgggcg cggaagcgg tgtctcgag gaacaaagg ttttacgaa gcgaccaag ctattggaa cagtttagg gatatcctg aacgaagcg aatctccta tcaaaggtg gcggcgcac ggattgtca agcgagcag cgtgctatg agacacggg gagctcaaa cagttgaaa tcgctgaga tataagctc aagaatgag aaaaagtcg gagaggaag caactcaag tatgagatt tttagtggg gataaggag gaagatgaa actacggat ttacgggat gtggagttt cgcataact aatgatttt acccgactc cagaaacta ctaaatgag gcttcggct gatagttac aaagatttg gttttattg aaattgata ctaacaagt gggttgtac cctcaaatt gcggttgag gatgagttt aattcgagt aaaactgtg tcggaacgc ttgtaccac actaaaaac aaaaactac gttttcttg cgtcctttg agttatttt gcaacgaat ccggaggtt ctagagttg cataatgat gatatagag gtgccccct cctggctac ttcagtaag cgaccaatc agtaaaaaa caccaattg ttaatctac cagacgatc cttgagaca aaaaaggtc tatcttgtt aataccatg cgaatgcct gccatccaa actctgatg cttttcagc aaaactata gccagtaac aagactttg acaaagttt gtttttgac gatttcttg ctccttgat gtccctttt tacggccag ggtaaaaca ctgttaatg cgagcgttg aaactgaga cacaagtgg aaacacgaa cttgagtcc aaattgaag gacccgtcg cgcgatttt aacaaaaaa gagattttc tattttatc gacgatttg gtcaatttt gtcaacact gatgttaat tataacata aaacggcta ctaccggcc gatttgaag gaaatctac acgggggaa ccgggaggg gtcggagtc aacgaaaaa aatcccttt gaggagtct ttcaaaatt atgcaaaat aatgagtta ggaggggtt caaatgact gaaaatttc acatacggg tgtttgctg caagaagag tgggcctgt agtattgag gaaagggtt gaaacgacc cctttcgaa tgccagtat tgcaaggaa actaaattg gggctgtcg gtgtttaaa ataatacag catgaggag ttctgtcgc ccggagata gacaagcct gaggtggaa actgttgtt gttaaggcg aattctaaa gtttttcgt tgtgataag tgcggaaaa gacctggtt ttgaccccc gttgagatt ttgaagcac aaaaaggcg tgtaattag
- >TC015049 RA
gcccttatc taatcacca ctaagataa ttatcacta ttacgtgat agagataat tttaatcag taaaatgac caagctttg cgtcagacg tgaaccact tccagccct aaccttaaa ctttcccac taacaatta cactgtttt aagcccaaa aatgtactt aaaagtaca gttttacgc gaaaatgtg tgataaggg cccataata ttgcgtcac taatcctta tcattaaat ttatcacca ttacgtgaa cctaaccca aaagttcag agtttacaa taaacaatg tcgcgcaaa cggcgccac tcgccccca cgacccctc tcgtcctac gccactgag ttggccccc ttcctggac caccacgcc aacatccgt aacccgcac gatttctgg aaattccac gaaaagtac accacattg ggtaaaacc gaccgaagc aaaatcctc aatttgcaa ttcacatcc aacgacctc tacgacaaa ctcccagtg ctcgaccgg aagggggcc cgcatccgg atatcacgt gacgacttc caccatttc ctcacctca gttcgagtg taccaagac ttccaacag aagagcaaa tttgcgaag ttgcgcaaa ctgagacgg gggcaaagc gagctcccc atcgcgggg taccgccaa gagattttg gacaagttg gggggctgt cgggttatg ttgatcgcg ggggacacc gggtgtggg aaaagtacg caagtgcca cagtttgtg taccaaggg ggctataaa aagattgtg tgtacgcaa ccgaggcgg atcgcgtgt gtgtcgtta gcgaagcga gtggctcat gagactctg acggacttt aagagcacg gtggggtac cagattagg ttcgagaag agcaagaga gctgatact tctatagtc ttcatgacc gaagggcta ttattaaga caagcgcaa gaagaagac actttgaat tcatacgac gttattatt ttggacgaa gtgcatgag aggcatctc catggggat ttcctagtc gggatcatg aagtgcctc ctgtacaaa agacaagac tttaaatta atcttaatg tctgcaaca ataaatctc gacttgttt acaaactac ttcaagcga gagaagctc gaagttgtc cgagtgccc ggtcggcta tttccgatc gaaatcgtc tatagacct ataatccga gacccctac gaacgaaaa agggaaaaa cttgactgt accccttat ttgcaaata atccaaatg attgatgag aaataccaa ccgagccaa aaaggcgac ttgttgatt ttcctaaat gggtatagt gaaatatca acactagca gatgctgtt agtgaatat agccaagtt aagaagaac tggattgtg ctccaactc cactcaagt ctgagtctt gaggaacag gacaaagtc ttcgattat ccaccagag ggcgtccgc aagtgcatc ataagcacg aacattgcc gaaacttcg gtcacaatc gacggaatc cgattcgtt atagactcg gggaaagtc aaccgtatg acataccac acctctggt ggtgttaac aaattgacc gaaacaaca atatcacaa gacagcgct aaacaacgt tcgggaaga gccggccgg acaggccct ggcatttgc taccgcctc tactctgag gaagatttc aaaaacttc gaaattttc actcctgcc gaaatccat ctcgtaccc ttagacacc ctcttatta catatgatt tcgttgggg ttaaccgac ttgaaccac ttccccttc ctcgaaaaa cccagcgag aaaagcatc gaagaaggc gtggagaaa ttgaaattc acaggcgcc ttagaactc aaaatcaac tgtcttgcc ttaaccccg ctaggggac gctttgagc caactaccg gtcgatcta agcatcggc aaaatgcta gtcttgagc acagttttc ggtaatgtt aacgccgtc cttgcggtg gcggcactg ctcagcatc cagtcacct ctgacgcaa aacgcttac cgaaacact gaagcgcaa gacttgcgc cgagtattg gattcgaac catggtgac ccgatctcg atgcttaac tactacaaa gagtggctg tcggtaaaa cagagctct gtacccgtg aaatcacgg tacgagaaa ggcgagtcg tccaaagtt tgggcgcgg aagcggtgt ctcgaggaa caaaggttt tacgaagcg accaagcta ttggaacag tttagggat atcctgaac gaagcgaat ctcctatca aaggtggcg gcgcacgga ttgtcaagc gagcagcgt gctatgaga cacggggag ctcaaacag ttgaaatcg ctgagatat aagctcaag aatgagaaa aagtcggag aggaagcaa ctcaagtat gagattttt agtggggat aaggaggaa gatgaaact acggattta cgggatgtg gagtttcgc ataactaat gattttacc cgactccag aaactacta aatgaggct tcggctgat agttacaaa gatttggtt ttattgaaa ttgatacta acaagtggg ttgtaccct caaattgcg gttgaggat gagtttaat tcgagtaaa actgtgtcg gaacgcttg taccacact aaaaacaaa aactacgtt ttcttgcgt cctttgagt tattttgca acgaatccg gaggttcta gagttgcat aatgatgat atagaggtg ccccctcct ggctacttc agtaagcga ccaatcagt aaaaaacac caattgtta atctaccag acgatcctt gagacaaaa aaggtctat cttgttaat accatgcga atgcctgcc atccaaact ctgatgctt ttcagcaaa actatagcc agtaacaag actttgaca aagtttgtt tttgacgat ttcttgctc cttgatgtc cctttttac ggccagggt aaaacactg ttaatgcga gcgttgaaa ctgagacac aagtggaaa cacgaactt gagtccaaa ttgaaggac ccgtcgcgc gattttaac aaaaaagag attttctat tttatcgac gatttggtc aattttgtc aacactgat gttaattat aacataaaa cggctacta ccggccgat ttgaaggaa atctacacg ggggaaccg ggaggggtc ggagtcaac gaaaaaaat ccctttgag gagtctttc aaaattatg caaaataat gagttagga ggggttcaa atgactgaa aatttcaca tacgggtgt ttgctgcaa gaagagtgg gcctgtagt attgaggaa agggttgaa acgacccct ttcgaatgc cagtattgc aaggaaact aaattgggg ctgtcggtg tttaaaata atacagcat gaggagttc tgtcgcccg gagatagac aagcctgag gtggaaact gttgttgtt aaggcgaat tctaaagtt tttcgttgt gataagtgc ggaaaagac ctggttttg acccccgtt gagattttg aagcacaaa aaggcgtgt aattaggga gcggtgctt cttgtgttg taaatattt cttgtttaa ataaataag atcaacaga t
