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Gene information
General information
TC030720 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3289463..3295814
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030720
    iBeetle screen iB_10197

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random fluorescence not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030720 RA
      MMRNFAPSQ LKRQGSSEQ ASSPGILST KRTRNQSSE 
      NPSKIRKPL SQLNNTEVY ISDHEELIK KLSAPFKCP 
      DPNYQGCNS TRTLGLRRS VAPRPLYDP NTPNALILY 
      SPPEQSEHE KLKGDGPVL VHVVVDPSL SNILRPHQR 
      EGVKFMYDC VTGVQIPNS FGCIMADEM GLGKTLQCI 
      TLLWTLVRQ GPECKPTID KGIIVCPSS LVRNWSNEI 
      DKWLKGRLS CLIMDGGPD ARKKLTQFM QGFGRTAIP 
      VLIISYETF RMHAEILHK SEIGLVLCD EGHRLKNCE 
      NQTYKALMG LKARRRVLL SGTPIQNDL LEYFSLVHF 
      VNEGLLGSA QEFKKKFEN PILRGQDST ATDSERQKA 
      VERLKELSD LVNRCLIRR TSNLLTKYL PVKFEMIVI 
      CQLTPLQKQ IYLNYINSE GLRKGVLND VEVKASLSA 
      LASITTLKK LCNHPDLIM DKILEGGEG FEKSRHLLP 
      PNYNDKDVM PQLSGKLML LDCFLANLK NNYNDKVVL 
      VSNYTQTLD LFEKLCRKR GYRYVRLDG TMSIKKRAK 
      VVANFNDPE SGEFIFMLS SKAGGCGLN LIGANRLIM 
      FDPDWNPAN DDQAMARVW RDGQQKPCY IYRFLAAGT 
      IEEKIFQRQ AHKKALSST VVDNNEETA RHFSVAELR 
      DLFKLEQTD SDTHEKLKC KRCLGKIQT KNPSDEADC 
      TSDLQDWYH CWDKKGLAD MVLKQIWDL SKYISFVFH 
      QKSAKQEVK RVKEEEVEE DPDYDDKDD LDYSE
    CDS sequence
    • >TC030720 RA
      atgatgcgc aattttgca cccagtcag ttaaaacgt 
      caaggctct tccgagcaa gcaagctca ccaggaatt 
      ctttctact aaacgaact cggaatcaa tcaagtgaa 
      aacccttca aaaatacga aaaccttta tcacaatta 
      aacaacacc gaagtttac ataagtgac catgaggaa 
      ctcataaaa aaactttcc gcccccttt aaatgcccc 
      gaccccaac taccaaggc tgcaattcg acccgcacc 
      ctgggcctc cgtcgcagc gtcgcccct cgcccctta 
      tacgacccc aacaccccc aacgccctc atcctctac 
      tcgcccccc gagcaaagc gagcacgaa aaactgaaa 
      ggcgacggc cctgtcctc gtgcacgtc gttgtcgac 
      ccgtccttg agcaacatc ctgcgcccc catcagcgc 
      gaaggcgtc aaattcatg tacgactgt gtgaccggc 
      gtccaaatc cccaactcg ttcggctgc ataatggcc 
      gacgaaatg ggacttgga aaaacccta cagtgtatc 
      accctcttg tggaccttg gtccggcag ggccccgaa 
      tgcaagccg accatcgac aaggggatc atagtctgc 
      cccagctcc ctggtccgg aactggagc aacgagatc 
      gacaagtgg ctcaaaggc aggctgagc tgtctcatc 
      atggatggg ggccccgat gccaggaaa aaactgacc 
      cagtttatg caaggcttc ggccggacg gcaatccca 
      gtgttgatt atttcctac gagacgttt cggatgcat 
      gccgagatt ttgcacaaa agtgagatt ggattggtg 
      ttgtgtgac gaaggacac aggttgaaa aactgcgag 
      aatcagacg tataaagcc ttgatgggg ctcaaggcc 
      aggcggagg gttctgttg agcgggacg ccgattcag 
      aacgatttg ttagagtac ttcagtttg gtgcatttt 
      gtcaatgaa gggctgttg ggaagtgct caggagttt 
      aagaaaaag tttgagaac cccattctg agagggcag 
      gactccact gccacggac tcagaacga cagaaggcg 
      gttgaacgg cttaaggaa ttatcagat ttggtgaat 
      cgttgccta attaggcga acttccaac cttcttact 
      aaatatctc ccggtgaag ttcgaaatg attgtaatt 
      tgtcaactg accccgttg caaaaacaa atctacttg 
      aattacatc aactcggag ggtttgcgc aagggcgtg 
      ttgaacgac gttgaagtt aaagcctct ctgagtgcc 
      ttagccagc atcacaacg ctgaaaaag ttgtgcaac 
      caccctgat ttgatcatg gataagatt cttgaaggt 
      ggggaaggt ttcgaaaaa tcgcgccac ttgctcccg 
      cccaattat aatgataaa gatgtgatg ccgcaattg 
      tctggaaaa ttgatgttg cttgattgt ttcctagcc 
      aatttgaag aacaactac aatgataaa gtcgtgttg 
      gtgtcgaac tatacgcag actttggat ctgtttgag 
      aagttgtgc cggaagagg gggtaccgg tatgtgcgt 
      cttgacgga actatgagt attaagaag agggctaaa 
      gttgtggcg aattttaat gatccggag tcgggggag 
      tttattttt atgttgagt tcaaaggcg ggagggtgt 
      ggcttgaat ttgattggt gctaatcgg ttgattatg 
      tttgatccg gattggaat ccggcgaat gatgatcag 
      gctatggca agggtgtgg agggatggg cagcagaag 
      ccgtgctat atctacagg ttcttagcg gcggggaca 
      attgaggaa aaaattttc cagagacaa gctcataaa 
      aaagccctg agttcgact gtcgttgac aataatgag 
      gaaacggcg cgacacttt agcgtcgcc gaattgcgt 
      gatttgttt aaattggaa caaacggac tcggacaca 
      cacgaaaaa ctcaaatgt aaacggtgt ttaggtaaa 
      atacaaacg aaaaatccg tccgatgag gctgattgt 
      acgtcggat ttgcaagat tggtaccat tgttgggac 
      aaaaaaggg ctggcagat atggttctt aaacaaata 
      tgggatttg tccaaatac atttccttt gtgttccat 
      caaaaatca gccaaacag gaagttaaa cgagttaag 
      gaagaagaa gtggaggaa gatcctgat tacgatgat 
      aaggatgat ttggattat tcggaatag
    mRNA sequence
    • >TC030720 RA
      aacgatcgt ctcattctc gcgggaagt gtgttttca 
      taatcataa cctaagaat ttaaacaac aaagaaaga 
      tgatgcgca attttgcac ccagtcagt taaaacgtc 
      aaggctctt ccgagcaag caagctcac caggaattc 
      tttctacta aacgaactc ggaatcaat caagtgaaa 
      acccttcaa aaatacgaa aacctttat cacaattaa 
      acaacaccg aagtttaca taagtgacc atgaggaac 
      tcataaaaa aactttccg cccccttta aatgccccg 
      accccaact accaaggct gcaattcga cccgcaccc 
      tgggcctcc gtcgcagcg tcgcccctc gccccttat 
      acgacccca acaccccca acgccctca tcctctact 
      cgccccccg agcaaagcg agcacgaaa aactgaaag 
      gcgacggcc ctgtcctcg tgcacgtcg ttgtcgacc 
      cgtccttga gcaacatcc tgcgccccc atcagcgcg 
      aaggcgtca aattcatgt acgactgtg tgaccggcg 
      tccaaatcc ccaactcgt tcggctgca taatggccg 
      acgaaatgg gacttggaa aaaccctac agtgtatca 
      ccctcttgt ggaccttgg tccggcagg gccccgaat 
      gcaagccga ccatcgaca aggggatca tagtctgcc 
      ccagctccc tggtccgga actggagca acgagatcg 
      acaagtggc tcaaaggca ggctgagct gtctcatca 
      tggatgggg gccccgatg ccaggaaaa aactgaccc 
      agtttatgc aaggcttcg gccggacgg caatcccag 
      tgttgatta tttcctacg agacgtttc ggatgcatg 
      ccgagattt tgcacaaaa gtgagattg gattggtgt 
      tgtgtgacg aaggacaca ggttgaaaa actgcgaga 
      atcagacgt ataaagcct tgatggggc tcaaggcca 
      ggcggaggg ttctgttga gcgggacgc cgattcaga 
      acgatttgt tagagtact tcagtttgg tgcattttg 
      tcaatgaag ggctgttgg gaagtgctc aggagttta 
      agaaaaagt ttgagaacc ccattctga gagggcagg 
      actccactg ccacggact cagaacgac agaaggcgg 
      ttgaacggc ttaaggaat tatcagatt tggtgaatc 
      gttgcctaa ttaggcgaa cttccaacc ttcttacta 
      aatatctcc cggtgaagt tcgaaatga ttgtaattt 
      gtcaactga ccccgttgc aaaaacaaa tctacttga 
      attacatca actcggagg gtttgcgca agggcgtgt 
      tgaacgacg ttgaagtta aagcctctc tgagtgcct 
      tagccagca tcacaacgc tgaaaaagt tgtgcaacc 
      accctgatt tgatcatgg ataagattc ttgaaggtg 
      gggaaggtt tcgaaaaat cgcgccact tgctcccgc 
      ccaattata atgataaag atgtgatgc cgcaattgt 
      ctggaaaat tgatgttgc ttgattgtt tcctagcca 
      atttgaaga acaactaca atgataaag tcgtgttgg 
      tgtcgaact atacgcaga ctttggatc tgtttgaga 
      agttgtgcc ggaagaggg ggtaccggt atgtgcgtc 
      ttgacggaa ctatgagta ttaagaaga gggctaaag 
      ttgtggcga attttaatg atccggagt cgggggagt 
      ttattttta tgttgagtt caaaggcgg gagggtgtg 
      gcttgaatt tgattggtg ctaatcggt tgattatgt 
      ttgatccgg attggaatc cggcgaatg atgatcagg 
      ctatggcaa gggtgtgga gggatgggc agcagaagc 
      cgtgctata tctacaggt tcttagcgg cggggacaa 
      ttgaggaaa aaattttcc agagacaag ctcataaaa 
      aagccctga gttcgactg tcgttgaca ataatgagg 
      aaacggcgc gacacttta gcgtcgccg aattgcgtg 
      atttgttta aattggaac aaacggact cggacacac 
      acgaaaaac tcaaatgta aacggtgtt taggtaaaa 
      tacaaacga aaaatccgt ccgatgagg ctgattgta 
      cgtcggatt tgcaagatt ggtaccatt gttgggaca 
      aaaaagggc tggcagata tggttctta aacaaatat 
      gggatttgt ccaaataca tttcctttg tgttccatc 
      aaaaatcag ccaaacagg aagttaaac gagttaagg 
      aagaagaag tggaggaag atcctgatt acgatgata 
      aggatgatt tggattatt cggaatagt tgtattttc 
      tatctgttg ttaaataat aaatacgga ttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY