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Gene information
General information
TC015111 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 3106461..3117134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015111
    iBeetle screen iB_10195

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015111 RA
      MTQIDEISN LTPIQEFYS GTNIFITGG TGFLGTILI 
      EKLLRSCND ISTIYILAR NKKGKNLQS RIDELFDDS 
      IFDRLKKEF PKFRHKVVG IGGDCSLPD LGISQQDRQ 
      VLINEVSVI FHVAATVRF DEKLKMAVA INVRAPQDM 
      LKLAHEMPH LKSMVHVST AYSNVPNKV IEERLYPVP 
      VDSKKLILM AETLGDNVL DNITPMVLD KWPNTYTYT 
      KAVAEDIVR QDGLDLPIG IYRPSIVVS TYQEPTEAW 
      INNLYGPTG VCAGAGSGI LRALHANSE VNANIVPVD 
      MCVNSLIAT AWDVGVKFE NSKKSCNKY EIPVYHFES 
      SNDQPLNWG RFMRLSYSH GKKTPSVKA IWYYTFNLY 
      KNYYAYLIA TIFLHYLPA LFVDGALLC MGKSPKMLR 
      IYKKIHKFT SVISYFSTR TWIFQSSNV QKMIERMSE 
      ADQKIFFCD LKKLDWNKF FATYLRGIR IYLLQDPIE 
      TLEEAHIRW NRLYWLHQG VKALVAFLL IRVLWWILS 
      TVFIY
    CDS sequence
    • >TC015111 RA
      atgactcaa attgacgaa atttcgaat ttgactccg 
      atccaggaa ttttactcc gggaccaac attttcata 
      acaggtggc actggtttt ttgggcaca attctcatc 
      gagaaattg ctgcgttct tgcaacgac atctcgacg 
      atttacatc cttgctcgt aacaagaag ggaaaaaat 
      ctccaaagt cgcatcgac gaattgttt gacgattcg 
      attttcgac cgattgaag aaagaattt ccgaaattc 
      aggcacaag gttgtgggg attggaggc gactgcagt 
      ctccccgat ttggggatc agtcaacag gaccgtcaa 
      gtgctcatc aatgaggtc tccgttata ttccatgtg 
      gcggcaacc gtccgtttc gacgaaaaa ctgaaaatg 
      gctgtggcc ataaacgtc cgcgcccct caagacatg 
      ctcaaactt gcacacgaa atgccacat ttgaagtca 
      atggtgcac gtttcgacg gcttattca aatgttccg 
      aataaggtc attgaagaa cgactgtat cctgtgcca 
      gttgatagc aagaaactt attcttatg gccgaaact 
      ttgggtgat aacgtcttg gacaacatc actccaatg 
      gttttggat aagtggcca aacacatac acttatacg 
      aaagctgtt gcagaggat atagttcga caagatggg 
      ctagatttg cccattggg atctacaga ccatctatt 
      gtggtgtca acatatcaa gaaccgact gaagcctgg 
      attaacaac ctttacggt ccaactgga gtttgtgct 
      ggtgctgga agtggcatt cttcgtgca ttgcatgca 
      aatagtgaa gttaatgca aatatcgtc ccagttgat 
      atgtgtgtc aattctttg attgccact gcttgggat 
      gtgggagtt aaatttgaa aattccaaa aagagttgc 
      aacaaatac gaaattccc gtttaccat tttgagtca 
      agtaatgat caacccttg aactgggga cgatttatg 
      cgtttatct tactcacat ggaaaaaaa acaccaagt 
      gttaaagcc atttggtac tatacgttc aatttgtac 
      aaaaattac tatgcctat ctcatagcc acgattttt 
      ctgcattat ttgccagcg cttttcgtc gatggagcg 
      ctgctgtgc atgggaaaa tcaccaaaa atgctccga 
      atttacaaa aagatccat aaattcacg agcgtaatc 
      tcgtacttc tcgacccgc acttggata ttccagtcg 
      tcaaacgtg caaaaaatg atcgagcgg atgtctgaa 
      gctgaccag aaaattttc ttctgtgac ttgaaaaaa 
      cttgactgg aataaattt ttcgctacg tatcttcga 
      ggaatacga atttacctc ctgcaagac ccgattgag 
      actttggag gaggcccac atcagatgg aacagactg 
      tattggttg caccaggga gttaaggca cttgtggct 
      tttctcctc atcagagtt ttatggtgg atattatcg 
      actgtattt atttactaa
    mRNA sequence
    • >TC015111 RA
      taacacaat aaaatcacc cgcggaacc tacacttcg 
      tttacaaag tcgacgaat ctagtcgct ggcacttga 
      cgcgatacc atcaaaaga attcttttg gttggccac 
      aggttactg caacagtgt ggtggtgta tgtgcttta 
      aaaccgaca gtttcggaa aaaatgact caaattgac 
      gaaatttcg aatttgact ccgatccag gaattttac 
      tccgggacc aacattttc ataacaggt ggcactggt 
      tttttgggc acaattctc atcgagaaa ttgctgcgt 
      tcttgcaac gacatctcg acgatttac atccttgct 
      cgtaacaag aagggaaaa aatctccaa agtcgcatc 
      gacgaattg tttgacgat tcgattttc gaccgattg 
      aagaaagaa tttccgaaa ttcaggcac aaggttgtg 
      gggattgga ggcgactgc agtctcccc gatttgggg 
      atcagtcaa caggaccgt caagtgctc atcaatgag 
      gtctccgtt atattccat gtggcggca accgtccgt 
      ttcgacgaa aaactgaaa atggctgtg gccataaac 
      gtccgcgcc cctcaagac atgctcaaa cttgcacac 
      gaaatgcca catttgaag tcaatggtg cacgtttcg 
      acggcttat tcaaatgtt ccgaataag gtcattgaa 
      gaacgactg tatcctgtg ccagttgat agcaagaaa 
      cttattctt atggccgaa actttgggt gataacgtc 
      ttggacaac atcactcca atggttttg gataagtgg 
      ccaaacaca tacacttat acgaaagct gttgcagag 
      gatatagtt cgacaagat gggctagat ttgcccatt 
      gggatctac agaccatct attgtggtg tcaacatat 
      caagaaccg actgaagcc tggattaac aacctttac 
      ggtccaact ggagtttgt gctggtgct ggaagtggc 
      attcttcgt gcattgcat gcaaatagt gaagttaat 
      gcaaatatc gtcccagtt gatatgtgt gtcaattct 
      ttgattgcc actgcttgg gatgtggga gttaaattt 
      gaaaattcc aaaaagagt tgcaacaaa tacgaaatt 
      cccgtttac cattttgag tcaagtaat gatcaaccc 
      ttgaactgg ggacgattt atgcgttta tcttactca 
      catggaaaa aaaacacca agtgttaaa gccatttgg 
      tactatacg ttcaatttg tacaaaaat tactatgcc 
      tatctcata gccacgatt tttctgcat tatttgcca 
      gcgcttttc gtcgatgga gcgctgctg tgcatggga 
      aaatcacca aaaatgctc cgaatttac aaaaagatc 
      cataaattc acgagcgta atctcgtac ttctcgacc 
      cgcacttgg atattccag tcgtcaaac gtgcaaaaa 
      atgatcgag cggatgtct gaagctgac cagaaaatt 
      ttcttctgt gacttgaaa aaacttgac tggaataaa 
      tttttcgct acgtatctt cgaggaata cgaatttac 
      ctcctgcaa gacccgatt gagactttg gaggaggcc 
      cacatcaga tggaacaga ctgtattgg ttgcaccag 
      ggagttaag gcacttgtg gcttttctc ctcatcaga 
      gttttatgg tggatatta tcgactgta tttatttac 
      taatggaaa agtattttc attacttta gtgatatta 
      ttttgtaat tttaacagt ttgtataat ttatataat 
      aaagctaat acggaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY