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Gene information
General information
TC015133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2794651..2795946
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015133
    iBeetle screen iB_10193

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015133 RA
      MEETFMLIA LILVMLIGS FLAGSIPLF FSFSEDKLK 
      RITAFGAGL LVGTALVVI IPEGVRSLL SEQLKEPQK 
      NHEKDVLSV IGISLVLGF IFMLMVDQI SQRKNENLN 
      PSERNITAT IGLVVHAAA DGVALGAAA TTSHADVEI 
      IVFLAIMLH KAPAAFGLV TFLLHEGID RQRIRKHLL 
      VFSLAAPVL AVCTYFGIG QKQKETLSS LNATGIAML 
      FSAGTFLYV ATVHVLAEL TQTSHHSYS KLQSLESGK 
      ASSQSLKTL ELICLVVGC LTPLLLTVG HHH
    CDS sequence
    • >TC015133 RA
      atggaagaa acatttatg ttaatagcg ttaatactg 
      gttatgcta ataggatca ttccttgct ggttcaatt 
      ccactgttt ttcagtttt tccgaggac aaattgaag 
      cgaattacg gcctttggg gccggtctt ttggtcgga 
      acagctctg gttgtgata ataccggaa ggggtgcgt 
      tcattactc agcgaacag ctcaaagaa ccacagaaa 
      aatcacgaa aaagacgtt ctttcagtc attggaatt 
      agtctagtg ttgggtttt atttttatg ttgatggtg 
      gatcaaatt tcccagcgg aaaaatgag aatttgaat 
      ccatccgag cgcaatatt actgccact attggtcta 
      gtggtccat gcggctgct gacggtgtg gcactgggg 
      gctgccgcc accaccagt catgccgac gtcgaaata 
      atcgttttt ctggcgatt atgttgcat aaagcgccc 
      gcagctttc ggtctagtc acgtttttg ctacacgaa 
      ggcattgat aggcaaaga attcgcaaa catttgttg 
      gtgttttca ctggcggca ccagttttg gccgtttgt 
      acttatttc gggattgga cagaagcag aaagagacg 
      ctgagttcg cttaatgca actggtatt gcaatgtta 
      ttttccgcc ggtacattc ctttacgtc gctacggtg 
      catgtctta gccgaactg acgcaaact tcgcatcat 
      tcctattcc aaacttcaa agtctagaa agtggcaaa 
      gcttcttcg caatcatta aaaacacta gaattgatc 
      tgtttggtt gttggatgt ttgactccc ttacttttg 
      actgtagga catcatcat tga
    mRNA sequence
    • >TC015133 RA
      tgctttaca cgtcaaacg tcagataag caaatttct 
      aggttagga aagtttatt tagaaaaaa ttgtttacg 
      ttgaaagta aaatattgt gatacgtat gcattttaa 
      aacacaaaa cattagtta cgttttaaa tttgcataa 
      cagtgagag gtagtacat aaaaatgga agaaacatt 
      tatgttaat agcgttaat actggttat gctaatagg 
      atcattcct tgctggttc aattccact gtttttcag 
      tttttccga ggacaaatt gaagcgaat tacggcctt 
      tggggccgg tcttttggt cggaacagc tctggttgt 
      gataatacc ggaaggggt gcgttcatt actcagcga 
      acagctcaa agaaccaca gaaaaatca cgaaaaaga 
      cgttctttc agtcattgg aattagtct agtgttggg 
      ttttatttt tatgttgat ggtggatca aatttccca 
      gcggaaaaa tgagaattt gaatccatc cgagcgcaa 
      tattactgc cactattgg tctagtggt ccatgcggc 
      tgctgacgg tgtggcact gggggctgc cgccaccac 
      cagtcatgc cgacgtcga aataatcgt ttttctggc 
      gattatgtt gcataaagc gcccgcagc tttcggtct 
      agtcacgtt tttgctaca cgaaggcat tgataggca 
      aagaattcg caaacattt gttggtgtt ttcactggc 
      ggcaccagt tttggccgt ttgtactta tttcgggat 
      tggacagaa gcagaaaga gacgctgag ttcgcttaa 
      tgcaactgg tattgcaat gttattttc cgccggtac 
      attccttta cgtcgctac ggtgcatgt cttagccga 
      actgacgca aacttcgca tcattccta ttccaaact 
      tcaaagtct agaaagtgg caaagcttc ttcgcaatc 
      attaaaaac actagaatt gatctgttt ggttgttgg 
      atgtttgac tcccttact tttgactgt aggacatca 
      tcattgaaa catgtttta ttgtattta tttattcga 
      ataaatgtc ataatttat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY