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Gene information
General information
TC015575 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2820092..2821659
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015575
    iBeetle screen iB_10192

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015575 RA
      MGDSKAPKA YQTRRITFS ACHRLHSPH 
      LSDAENAQI YGKCNHING HGHNYIVKV 
      TLFGAIDPK TGMILNMTE LKKYMEEAI 
      MQPMDHKNL DKDVDFFKT RPSTTENLT 
      VFIWERMKK VMERPDLLY EVLVYETEN 
      NIVKYRGE
    CDS sequence
    • >TC015575 RA
      atgggtgac tcaaaggcc cccaaagcc taccaaact 
      cgcagaatc accttttcg gcctgccac cgattacac 
      agcccccat ttgagcgac gctgagaac gcccaaatt 
      tacggcaaa tgcaaccac attaacggc catggacac 
      aattacatt gtaaaagtg acccttttt ggggcgatt 
      gacccgaaa accggcatg attttgaac atgactgaa 
      ctaaagaag tacatggaa gaggctata atgcaacca 
      atggaccac aaaaatcta gacaaagat gtagatttt 
      ttcaaaact aggccaagt actacggag aatttgacc 
      gtttttata tgggagagg atgaaaaag gtgatggaa 
      aggccggat ttgttgtac gaggttttg gtttatgaa 
      accgaaaat aacattgtc aaataccgg ggcgaataa
    mRNA sequence
    • >TC015575 RA
      ccgcttcta taaaagtgt tattctagg taggcaacc 
      aacattaca acggcagcg atcgtctgt tccacagtg 
      aaattacca cataaatac ttaaaattt ttaccaaaa 
      aatgggtga ctcaaaggc ccccaaagc ctaccaaac 
      tcgcagaat caccttttc ggcctgcca ccgattaca 
      cagccccca tttgagcga cgctgagaa cgcccaaat 
      ttacggcaa atgcaacca cattaacgg ccatggaca 
      caattacat tgtaaaagt gaccctttt tggggcgat 
      tgacccgaa aaccggcat gattttgaa catgactga 
      actaaagaa gtacatgga agaggctat aatgcaacc 
      aatggacca caaaaatct agacaaaga tgtagattt 
      tttcaaaac taggccaag tactacgga gaatttgac 
      cgtttttat atgggagag gatgaaaaa ggtgatgga 
      aaggccgga tttgttgta cgaggtttt ggtttatga 
      aaccgaaaa taacattgt caaataccg gggcgaata 
      atctttatt tctataatg tgatacatt gtaaaattg 
      taacaacat tccttgtat tttttcgaa taaacatat 
      ttttttaat aaaattgat tctgaaacc ctgcaacca 
      aacaaacac tggtcatta cctggagcc aatttaact 
      cgaaattat ttcctttgc ccagatgtg gcaaattat 
      taattagtt acgtaacgt ccaaattgg agcgatttt 
      tccaattat ctcatttaa aatttctac tcttccata 
      agcgcttac gcaatgaat catcaaaac ggtcaaaat 
      tttgaaaaa gcggcaaaa tctcgttat tcgtttggg 
      caaccgaaa tattcagta attaatttt ctcgtgcta 
      gcgttaagg aaagtcaga cccgtatga gtaattatt 
      attttgtaa caaatatat tatttctga actttatac 
      gtagtacct gcgcaatcg atacacaag acaagatgc 
      tgaataatt tacaaaagt tatataact tttaaataa 
      atccaaagt gggacaata cgatggttc gataattgc 
      aaatccgag tcgagaaaa tgccgcaat aaattcaat 
      ttcttcact ggctttttc taaaaagaa aatgcgaag 
      acacgtgca ccagataaa cccgattac tggagaacc 
      tgtttgttg tataaccaa agaggccaa gaccatgaa 
      aagtgtctc actacaacg agaaaaaaa cggcgcttt 
      cttatctta tataatacg tcgcgattt tgatgaatt 
      ctcggcgat ttttaatga aagtcaacg agaatcgca 
      cttttctgt aattgattt atgggcctg tgagaatcg 
      gaatgtaag aacacaact ttcacgcac acgtatctc 
      aaaaattgc tcaaaattc atgaattca ctcagacgg 
      caattatac aagaaaaca atgcaatta gc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY