Status
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Gene information
General information
TC014227 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3498494..3501423
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014227
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10191

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014227 RA
      METERKEHR LVLPVPMYH NDYNIWSVL KNCIGKELS 
      KITMPVVFN EPLSFLQRM AEYMEYSHL LRLASQQQD 
      PIIRMQYVI GFAVSALAS NWERLGKPF NPLLGETYE 
      LEREEFKII CEQVSHHPP VSAFHAESK DWVFHGSIH 
      PKLKFWGKS VEIQPKGTV TVEFPKLNE AYTWSNVQC 
      CVHNIIVGK LWMEQCGTM EIVNHSTGH KAHLTFKPA 
      GWNSKDLHR VEGYIADKS KHKLFFIYG KWTEFIKCT 
      DYASYEDYL KENSHKFKR DGDKSKSPN DSPSHTSRK 
      VLQKLNSLK VSPFKSNSS QESVDECAP PDVPDGMVP 
      KSDSTYSID IPNSVTIWK VKPRPLQSS DYYQFTEFA 
      MSLNELEPG MDTSLCPTD SRLRPDIRK LEQGDIDGA 
      AIEKTRLEE KQRDANKHR KSKKGTEWS PRWFNSGIN 
      PHTKQEDWV YNGNYWKRE YTDNPDIF
    CDS sequence
    • >TC014227 RA
      atggaaacc gaacggaag gaacatagg ctcgttctg 
      ccagtgccg atgtatcac aatgactac aacatttgg 
      tcagtatta aaaaactgt attgggaaa gaattgagc 
      aaaatcacg atgccagtg gtatttaac gaaccatta 
      agcttcctg caaaggatg gctgaatac atggaatat 
      tcccacctg ctacgtttg gcgtcacag cagcaagac 
      cccataatt cgaatgcag tatgtcata ggtttcgcc 
      gtcagtgct ctagcatca aactgggaa cgtctcggc 
      aaaccgttt aatcctctc ttgggcgaa acttacgag 
      ttggaaaga gaagagttt aagataatt tgtgaacaa 
      gtcagtcac cacccgcca gtctcggct tttcacgct 
      gaaagcaaa gactgggtt ttccacggt tcaatccac 
      ccgaagttg aaattctgg ggaaaaagc gtcgagatc 
      caaccgaag ggaacagtc actgtcgag ttccctaaa 
      ctaaacgag gcctacact tggagcaac gttcagtgc 
      tgcgtccac aacataatc gttgggaaa ttatggatg 
      gaacagtgc gggacaatg gaaattgtc aatcactcg 
      actggacac aaggcccat ttgacgttc aagcccgca 
      ggctggaac tccaaggac ctgcaccgc gtcgaaggc 
      tacattgcg gacaaatcg aagcataaa ttatttttc 
      atttacggg aaatggacc gaattcatc aaatgcaca 
      gactatgct tcctacgag gactacttg aaggaaaac 
      tcgcacaaa ttcaagcgc gacggtgac aagagcaaa 
      agcccgaac gattcccca tctcacact tcccggaaa 
      gttttgcaa aaactgaac agcttgaaa gtcagccct 
      ttcaagtca aactcgagc caagagagt gtcgatgag 
      tgtgcccct ccagacgtc ccggatggc atggttcca 
      aagagcgat tccacctac tccattgac attcccaat 
      tcggtgacg atctggaaa gtcaagcct cggcccttg 
      cagagcagt gactattac cagtttacc gaattcgcc 
      atgtctttg aacgagctg gagcccgga atggacacg 
      agtctgtgt ccaaccgac tcgcgcctt cggcccgac 
      attcgaaag ctggagcag ggcgacatc gacggagcc 
      gctatcgaa aaaacccgt ctggaggag aaacaaaga 
      gacgcgaat aagcaccgc aagagcaag aaagggaca 
      gagtggagt cccaggtgg ttcaactcg gggattaat 
      ccgcacacc aagcaggag gattgggtc tacaatggg 
      aattactgg aagagggag tacaccgat aatcccgat 
      atattttaa
    mRNA sequence
    • >TC014227 RA
      gcgcttttg aagtcgtgt ttcgggggc ggttggtgg 
      caaacagag aaggttgta ggtttctgg ggacgaagg 
      gtgtgctaa tcgattcga agcaaagct gaaaaatgt 
      tagaaatgt gaatgcgtt gactcgtgt ccaggtagg 
      tggtgttaa gtgagtcgt ttcgagttg atttgaacc 
      gattctgct tgtggttct tttgatttt aacacatag 
      gtccattgt ttcatatcg accatcgcc tgtcattaa 
      ataaattca tgtttgggt cgtttgttg ttaaatgtg 
      gtaatttga ccgtgtgag gcgtttagg aattctgct 
      ccaatccgg acgtttttt aggagcatt ttagttgat 
      caatcaaag gatatgatg ctgcttatt tgataattt 
      ggattatcc ttaattgat cggatgtgc gattcactg 
      ttacttttt tttgggggg attgtctcg gttagagca 
      gaatggaaa ccgaacgga aggaacata ggctcgttc 
      tgccagtgc cgatgtatc acaatgact acaacattt 
      ggtcagtat taaaaaact gtattggga aagaattga 
      gcaaaatca cgatgccag tggtattta acgaaccat 
      taagcttcc tgcaaagga tggctgaat acatggaat 
      attcccacc tgctacgtt tggcgtcac agcagcaag 
      accccataa ttcgaatgc agtatgtca taggtttcg 
      ccgtcagtg ctctagcat caaactggg aacgtctcg 
      gcaaaccgt ttaatcctc tcttgggcg aaacttacg 
      agttggaaa gagaagagt ttaagataa tttgtgaac 
      aagtcagtc accacccgc cagtctcgg cttttcacg 
      ctgaaagca aagactggg ttttccacg gttcaatcc 
      acccgaagt tgaaattct ggggaaaaa gcgtcgaga 
      tccaaccga agggaacag tcactgtcg agttcccta 
      aactaaacg aggcctaca cttggagca acgttcagt 
      gctgcgtcc acaacataa tcgttggga aattatgga 
      tggaacagt gcgggacaa tggaaattg tcaatcact 
      cgactggac acaaggccc atttgacgt tcaagcccg 
      caggctgga actccaagg acctgcacc gcgtcgaag 
      gctacattg cggacaaat cgaagcata aattatttt 
      tcatttacg ggaaatgga ccgaattca tcaaatgca 
      cagactatg cttcctacg aggactact tgaaggaaa 
      actcgcaca aattcaagc gcgacggtg acaagagca 
      aaagcccga acgattccc catctcaca cttcccgga 
      aagttttgc aaaaactga acagcttga aagtcagcc 
      ctttcaagt caaactcga gccaagaga gtgtcgatg 
      agtgtgccc ctccagacg tcccggatg gcatggttc 
      caaagagcg attccacct actccattg acattccca 
      attcggtga cgatctgga aagtcaagc ctcggccct 
      tgcagagca gtgactatt accagttta ccgaattcg 
      ccatgtctt tgaacgagc tggagcccg gaatggaca 
      cgagtctgt gtccaaccg actcgcgcc ttcggcccg 
      acattcgaa agctggagc agggcgaca tcgacggag 
      ccgctatcg aaaaaaccc gtctggagg agaaacaaa 
      gagacgcga ataagcacc gcaagagca agaaaggga 
      cagagtgga gtcccaggt ggttcaact cggggatta 
      atccgcaca ccaagcagg aggattggg tctacaatg 
      ggaattact ggaagaggg agtacaccg ataatcccg 
      atatatttt aagccagac gtagcgatc gattaggta 
      gaacttagc atttattgt aaatcattc ataagtttt 
      gggacgtgg caatgttga gagtatcta atccctagc 
      ctgatttat ttccgggaa cgaacgatt ttgccttga 
      tgatttcgt agacaacgt gctcaagga tcagtacct 
      tcgcgcttg gtcatagga aacgttaca tagtatcag 
      tgtctgtgc caaagcaca ataccatca tagttaaat 
      tgtatagcg cttaatgct tccaaaaac tggaaatac 
      gtatctacc tgtccttaa aatatcacc catcatgca 
      ataactccc agacaagga cttagtccc tgtttgttg 
      atatcagtg tgttgttca cgaactagt aggtctatt 
      cgatgaaat tggcgactc attacagtt gccatatga 
      tattttcgt aatatttta ggacactta ataggttta 
      gttaccagt tgaagtaat tgttgcaaa taattgtct 
      atataaagt agctagtgg cctgatatt cccacttat 
      ttaaaaatt gttttcgta caactaatt tcgtttgtt 
      acgaaggaa ctataaagt caatatctt gggatcatt 
      cgatataca gtgatgtag gtaaaatta aggtatatt 
      gaaaattat gtttgaatc tgtacatca ttaccaata 
      aatatttta ttatgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY