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Gene information
General information
TC015126 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2695654..2704731
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015126
    iBeetle screen iB_10190

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015126 RA
      MVTPPQQFC VRWNSYQSN LQNAFPKLL NSEHFVDVT 
      LACENEMLK CHKVVLSAC STYFEKLLL DNPCQHPII 
      FMKDMKFQE MQSLVDFMY KGEVNVTQD DLPSLLKSA 
      EALQIRGLC GSDQLLSQQ QLGALKPQS KPVLNNVDK 
      NHEMAKPAE VKKEDKIES SENNPSSSE CDSNDGVQS 
      KANARVTPS NIQRIVFPE HELKEEVDN DSYYEGESD 
      LMDTEFLEE DVAINTNDL NKTPEFAIA NVSCQYDGS 
      PGSATNRRI RRSDEELRR AAECITRGQ TFQTVSDQF 
      NIPISTIRF YMARKGILP RRKRGRACS GPTGLPMPV 
      QTYHSPGNT SPVGPPYHI VHYKLPTLG VSKLK
    CDS sequence
    • >TC015126 RA
      atggtgacg cctccgcaa caattctgc gttcgctgg 
      aacagctac cagtcgaat ttgcaaaac gccttcccg 
      aagctgctc aattccgag catttcgtc gacgttacg 
      ttggcctgc gagaatgag atgctgaag tgccacaag 
      gtggtcctg tcagcgtgc tccacgtat ttcgagaaa 
      cttctccta gacaatcct tgccagcac cccattatt 
      ttcatgaaa gatatgaag tttcaggag atgcagtca 
      ctggtcgac tttatgtac aaaggcgaa gtgaacgta 
      actcaggac gatttacct tcattgttg aaaagcgca 
      gaggccttg caaatccgg ggcttatgt gggtctgat 
      cagttgtta agccagcaa cagttgggg gccctcaag 
      ccacaatca aaaccggtc ctcaataac gtagataag 
      aaccacgaa atggccaaa ccggccgaa gttaaaaaa 
      gaggataaa atcgaaagt tcggaaaat aatccgtcg 
      tcgtccgag tgcgattcg aacgacggg gtccaaagc 
      aaagctaac gccagagtc acgccttca aacatccaa 
      agaattgtt ttcccggaa catgaattg aaggaggaa 
      gtggataac gattcgtat tatgagggc gagagcgat 
      ctcatggat acggagttt ctcgaagaa gacgtcgcg 
      attaacacg aatgatctc aacaaaacg cccgagttt 
      gccatcgcc aacgtctcc tgccagtac gatggcagt 
      ccaggttcg gcgacgaat cggcgcatc cggcgctcg 
      gacgaggag ctgcgacgt gccgccgag tgcataacc 
      cgcggccaa accttccaa acggtcagt gatcagttc 
      aatatcccc atatcgaca atccgtttt tacatggcg 
      aggaaaggt attttgcct cgacgtaag cgaggaagg 
      gcttgttcg ggcccaacg ggactgccc atgcctgtc 
      cagacgtac cattcgcca ggtaacacc agtcccgtt 
      ggcccaccc taccatatc gtccattac aagttacca 
      acgttgggt gtttcaaaa cttaagtaa
    mRNA sequence
    • >TC015126 RA
      aactgtcaa ttgtggttc accccgtat ttagttttt 
      tcgcgattg gaaagcgtc ccgacgtca gttgcggcg 
      tcgcgtgat gtttatggt ggcgttcgt ggcgtcgtg 
      tgccccctt gtcgcgtga attagtgag aaattcccg 
      cgcgttgtt tgccgcgag tgtaccaag ccggcaaaa 
      ttgcaaatg gcgggggtc tatatataa atattatat 
      ttagaattg ttgcacgtt ttcggtttt gtggcggtt 
      cccgagggt gaaagtggg tgctagtgc gcgtcccgg 
      gggcggctt ttgcgtttt tgcggcgat tcgtgcggg 
      ttttggggc ggaaaagcg cgtccacgg cctgcttgt 
      ttatatttt aaaattgaa tagtacacc ccctggatc 
      tcggaaagt aaatattgt agtgcgccg gtgcaagca 
      cggacgtac gtcgcactc cttacatgc ttctaacgt 
      gcgtcttcg gcgacaccg tcgtcggcg actcaagtc 
      ggtgattgg gccttacaa gcaatataa ttatttaag 
      tgaacattt tgcattaaa agtttttat cgatttttt 
      acgcatttt tctcagtgc tggcgctta aataccggt 
      cttaaagca agggttttg gacgattgt acattgaaa 
      ttactattt ttggaggaa tatacaccg cccccttgc 
      tctaatgag tctcgagtg aagccgtca caagtagta 
      cgaaaatgg tgacgcctc cgcaacaat tctgcgttc 
      gctggaaca gctaccagt cgaatttgc aaaacgcct 
      tcccgaagc tgctcaatt ccgagcatt tcgtcgacg 
      ttacgttgg cctgcgaga atgagatgc tgaagtgcc 
      acaaggtgg tcctgtcag cgtgctcca cgtatttcg 
      agaaacttc tcctagaca atccttgcc agcacccca 
      ttattttca tgaaagata tgaagtttc aggagatgc 
      agtcactgg tcgacttta tgtacaaag gcgaagtga 
      acgtaactc aggacgatt taccttcat tgttgaaaa 
      gcgcagagg ccttgcaaa tccggggct tatgtgggt 
      ctgatcagt tgttaagcc agcaacagt tgggggccc 
      tcaagccac aatcaaaac cggtcctca ataacgtag 
      ataagaacc acgaaatgg ccaaaccgg ccgaagtta 
      aaaaagagg ataaaatcg aaagttcgg aaaataatc 
      cgtcgtcgt ccgagtgcg attcgaacg acggggtcc 
      aaagcaaag ctaacgcca gagtcacgc cttcaaaca 
      tccaaagaa ttgttttcc cggaacatg aattgaagg 
      aggaagtgg ataacgatt cgtattatg agggcgaga 
      gcgatctca tggatacgg agtttctcg aagaagacg 
      tcgcgatta acacgaatg atctcaaca aaacgcccg 
      agtttgcca tcgccaacg tctcctgcc agtacgatg 
      gcagtccag gttcggcga cgaatcggc gcatccggc 
      gctcggacg aggagctgc gacgtgccg ccgagtgca 
      taacccgcg gccaaacct tccaaacgg tcagtgatc 
      agttcaata tccccatat cgacaatcc gtttttaca 
      tggcgagga aaggtattt tgcctcgac gtaagcgag 
      gaagggctt gttcgggcc caacgggac tgcccatgc 
      ctgtccaga cgtaccatt cgccaggta acaccagtc 
      ccgttggcc caccctacc atatcgtcc attacaagt 
      taccaacgt tgggtgttt caaaactta agtaagagg 
      cggaccctt gattcattt tttttttat tttactttc 
      ttttttacg cgatctatt tatttttta tgatttttt 
      ttatacaaa gttcagtat tttaatttc gactgtact 
      tagattagg gattttttg ctgccaaat gatgttaat 
      ttttacgga ttcgactca atttcagtt attgataat 
      tataaggaa aatggaaaa ttgtcttct aagatatta 
      agatattgt cgtggttag aaaaaaata ttgtaggaa 
      gttgtgact taggttaat cggaaaatt ggtgaacta 
      gaattgtac aaaagaacc ttctgatat ttgaaaact 
      gtactagtc cgaaaagat gattatttt gtataacgt 
      gtgattttg cccatcact ttttttatt taattttta 
      gggctgcca ttcgtgata tttaaaatc attctcatc 
      tatttattt taaccatcg caaacgtca ccgtaatca 
      aaagtaggc ttccatcgt aatttaaga gaagaaaag 
      tatctcatt ttttattag gttaagacc ctaaccaaa 
      gcagttgac tgatagagt ggcagctta agagactaa 
      gcaatacca acacacaca caaaagaat tataattac 
      gcttatttt tttattttt attttgttc ccgatatct 
      tgcaaaacg gataataat gccagtgat acacacaga 
      gtaagtaat taagccata gaccgcact cacctcatt 
      ttatttatc gtgttttgt tttccgttt catggtcgt 
      aaaaataga caaaaactg agacaccac tgtttaggt 
      gccagattc tctgatctt tgtaaaaag gaaataaca 
      atcgtattt atcgagcgt gcacgcgct cgaaaccag 
      taattatta tcgataaga ctagtgata agtgatttg 
      ttagattta gaccagttt gtgttgatt ttgattttg 
      ttcttcact tcgttatgt atccggctc tcatttatc 
      gagttttta gttgtaggt tcgtgtatg atatgtagc 
      cgttcactt gaacaatta gtttaggga ttttccgaa 
      atttctttt gggattcat agtgatttt ttcgcagtt 
      ttcgattct tctccgtag aagacctcc tgtaaagta 
      tattatgtg tacaagtgc catctataa tatattttt 
      ctagttaaa tagaacgct tatgtcttg tgacttaga 
      cattgtata cagtattct gtcgtaata tacaagtat 
      tatatac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY