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Gene information
General information
TC015144 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2408337..2416456
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015144
    iBeetle screen iB_10189

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015144 RA
      MSAIEDEIS TADTEDALL KGSRNSSET ISEGTSKRP 
      WCYVIGGGV LLVWGLATL IWTPFEILM AERLRMTPG 
      FPPYEWWKN PPDEVIIRA YLYNVTNSE EFLNNPNVK 
      INMAEIGPI VFMEKLTHT NVTFNENGT MTYVATRKA 
      IFLPELNSI DLNATIVVP NWALLGIAS YLSEQSFFV 
      KLGFNLLTR SVKPQQFVK LTIDQFLWN FTDPMLDAA 
      YKIAPFLVP IKNLGILSR IYTNFTNTV TVYIGTKYG 
      DENFFLIDK YDGSKDLPN YGASCGGGV VNSSEGVAY 
      PQYITKNTT LKYWRKTFC KMAVLHHRK DVSKYGVNA 
      FRFDLIDSI FNRTEPSEA DCFRGQPDL PDGLSDISK 
      CHFGFPLAT SFPHFLHGD SIIRSYVSG MKPNRTKHS 
      SYLIVEPTT GVPLESAAR SQSNLVVRK LTGFNELVT 
      PFSDTVIPM FWLEYNQMG LPWYIIGLV YFQVNVLPI 
      FQRFFTAFL LTGSVILFC FYIKHKRKQ RLLDNKVLV 
      FEKELFMKK P
    CDS sequence
    • >TC015144 RA
      atgtctgcc atagaagac gagatcagc acggctgat 
      actgaagat gctctactc aaaggctcg agaaacagc 
      tcggagacg atcagcgag ggaacgtcg aagcgaccg 
      tggtgttac gtcatcggc ggcggggtt ctactcgtc 
      tgggggttg gcgacgctg atttggacg ccgtttgaa 
      atactaatg gccgaacgc ctccgaatg acgccgggg 
      tttcccccg tacgaatgg tggaaaaat cccccagat 
      gaagtcata atcagggcc tacctctac aacgtcact 
      aattccgaa gagttccta aataacccg aacgttaaa 
      ataaacatg gccgaaatc ggcccgatc gtcttcatg 
      gagaaattg acacatacc aacgtgacc ttcaacgaa 
      aatggcacc atgacctat gtggcaacg cgaaaggcg 
      atttttttg ccagaactg aatagcatt gatctgaat 
      gcgaccatc gtggtgcct aattgggct ctgctgggc 
      atcgcgtcg tatttgtcc gaacagagc tttttcgtt 
      aagttaggg tttaacctg ttgacgaga tcggttaaa 
      ccgcaacag tttgttaag ttaaccatt gatcagttc 
      ctgtggaac ttcacagat ccgatgctg gatgcggcc 
      tataaaatt gcgccgttt ctagttccg atcaaaaat 
      ctagggatc ttgtcgcgg atttacaca aatttcacc 
      aacactgta acagtgtac attgggacg aaatacggg 
      gacgagaat ttcttcctc attgataaa tacgacggc 
      tctaaggac ctccccaat tacggggct agttgtggg 
      ggcggagtc gtgaattca tcagagggc gtggcttac 
      cctcaatac atcaccaaa aacaccact cttaagtac 
      tggcggaag acattttgc aaaatggcg gtcctccac 
      catcgaaaa gatgtgtct aaatatggg gttaatgcg 
      ttccggttt gatttaatc gactccatt tttaaccga 
      acggagccg agcgaagcc gattgtttt cgggggcag 
      ccggattta cccgatggt ctttcggat atttccaaa 
      tgtcatttt gggtttccc ctagctacg agtttccct 
      cattttctc cacggtgac tcgattatt agatcgtac 
      gtcagtgga atgaagcca aaccggacg aagcactcg 
      tcttacctc atcgttgaa ccgaccaca ggggtgcca 
      ctcgaatcg gcggcccgg tcccaaagc aacctagtc 
      gtgcgtaaa ttgacaggt tttaatgaa ttagtcacg 
      cctttcagc gacactgtc attcctatg ttctggttg 
      gaatacaac cagatgggc ctaccttgg tatatcatc 
      ggcttggtc tatttccaa gtcaacgtt cttccgatt 
      tttcagcgc tttttcaca gccttctta ctgacggga 
      agcgtgatc ctcttctgt ttctatatc aagcacaag 
      cggaagcag cggctacta gataataag gttctcgtg 
      ttcgagaag gagttattt atgaagaaa ccctag
    mRNA sequence
    • >TC015144 RA
      ccctgtccc aaggtcacc cggacaaat ccggagtcg 
      caacactca ctcgcattt taaacgttg cgtttgtgc 
      ggtgctcac gtttggatt ccggtgctc tgctggtgc 
      ggcgttgcg ttcgcggag cccaaatct agtcgccct 
      acgatgctc cagcacgca cgcagctga atctagtcg 
      gccgagatg tctgccata gaagacgag atcagcacg 
      gctgatact gaagatgct ctactcaaa ggctcgaga 
      aacagctcg gagacgatc agcgaggga acgtcgaag 
      cgaccgtgg tgttacgtc atcggcggc ggggttcta 
      ctcgtctgg gggttggcg acgctgatt tggacgccg 
      tttgaaata ctaatggcc gaacgcctc cgaatgacg 
      ccggggttt cccccgtac gaatggtgg aaaaatccc 
      ccagatgaa gtcataatc agggcctac ctctacaac 
      gtcactaat tccgaagag ttcctaaat aacccgaac 
      gttaaaata aacatggcc gaaatcggc ccgatcgtc 
      ttcatggag aaattgaca cataccaac gtgaccttc 
      aacgaaaat ggcaccatg acctatgtg gcaacgcga 
      aaggcgatt tttttgcca gaactgaat agcattgat 
      ctgaatgcg accatcgtg gtgcctaat tgggctctg 
      ctgggcatc gcgtcgtat ttgtccgaa cagagcttt 
      ttcgttaag ttagggttt aacctgttg acgagatcg 
      gttaaaccg caacagttt gttaagtta accattgat 
      cagttcctg tggaacttc acagatccg atgctggat 
      gcggcctat aaaattgcg ccgtttcta gttccgatc 
      aaaaatcta gggatcttg tcgcggatt tacacaaat 
      ttcaccaac actgtaaca gtgtacatt gggacgaaa 
      tacggggac gagaatttc ttcctcatt gataaatac 
      gacggctct aaggacctc cccaattac ggggctagt 
      tgtgggggc ggagtcgtg aattcatca gagggcgtg 
      gcttaccct caatacatc accaaaaac accactctt 
      aagtactgg cggaagaca ttttgcaaa atggcggtc 
      ctccaccat cgaaaagat gtgtctaaa tatggggtt 
      aatgcgttc cggtttgat ttaatcgac tccattttt 
      aaccgaacg gagccgagc gaagccgat tgttttcgg 
      gggcagccg gatttaccc gatggtctt tcggatatt 
      tccaaatgt cattttggg tttccccta gctacgagt 
      ttccctcat tttctccac ggtgactcg attattaga 
      tcgtacgtc agtggaatg aagccaaac cggacgaag 
      cactcgtct tacctcatc gttgaaccg accacaggg 
      gtgccactc gaatcggcg gcccggtcc caaagcaac 
      ctagtcgtg cgtaaattg acaggtttt aatgaatta 
      gtcacgcct ttcagcgac actgtcatt cctatgttc 
      tggttggaa tacaaccag atgggccta ccttggtat 
      atcatcggc ttggtctat ttccaagtc aacgttctt 
      ccgattttt cagcgcttt ttcacagcc ttcttactg 
      acgggaagc gtgatcctc ttctgtttc tatatcaag 
      cacaagcgg aagcagcgg ctactagat aataaggtt 
      ctcgtgttc gagaaggag ttatttatg aagaaaccc 
      tagttttaa gttatacac gcagtattg taccattgc 
      tcaattatt ttctaagaa agtgtcatt ttctaaggg 
      gaaatttgt tgattttgt aacgtttta gtcgtaaat 
      taaataata gaccaaagt ttctcatta aatgtgata 
      cttttttgt tatgttggc agcaacatc tgcgacgct 
      aatcattag cttaggcaa tttttgaca ataaataaa 
      taaatcagc tacgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY