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Gene information
General information
TC015554 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2222653..2225720
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015554
    iBeetle screen iB_10184

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015554 RA
      MVVSMDRWV GKVAVVTGA STGIGAAIA KQLVSNGII 
      VVGIARRVE LVQELAQEL GDRKGKLHA YKADVSKED 
      DILKAFKWI EENLGPVHI LINNAGVAK ETTLVDGDT 
      EAWRNVLDV NVLGLCIAT REAIKSMKK HDIKGHIVH 
      INSLCGHRV PNFPGMNIY PASKHAVTA LTETLRQEL 
      NFVGSKIKI TSVSPGLVT TEMTTLNKN LDPEKKAIV 
      DKFPALETE DIADGVIYA LGTPEHVQV HELTIKPVG 
      EMY
    CDS sequence
    • >TC015554 RA
      atggtcgtg tctatggat agatgggtg ggcaaggtg 
      gctgtggtg acgggcgct agtacggga attggggcg 
      gcgattgcc aaacagctc gtttccaat gggattatt 
      gtcgttggc attgctcgg agagttgaa ctcgtccaa 
      gagctggcg caggaactg ggcgacagg aaaggtaaa 
      ctccacgcg tataaagcc gacgtttcg aaagaagac 
      gacatcctc aaggccttc aaatggatt gaggaaaat 
      ttgggcccc gtacacata ctaataaac aatgcagga 
      gttgctaaa gaaacaact ctagtagat ggcgatacc 
      gaagcttgg agaaacgtc cttgacgta aacgtcctt 
      ggtttatgt attgcaaca agagaggca atcaaaagc 
      atgaaaaaa catgatata aaagggcat atcgttcac 
      attaacagc ctgtgtggg catagagtg cccaatttt 
      ccaggcatg aatatttac ccagcttct aaacacgca 
      gtcactgca cttacagaa actttaaga caagaattg 
      aattttgtt ggctccaaa attaaaatc acgagcgtt 
      agtcctggt ctagtaacc actgaaatg acaactctc 
      aataaaaac ctagatcct gagaagaag gcaattgtt 
      gataagttt ccagctctg gagactgaa gatatcgca 
      gatggcgtt atctacgct ttgggcacc cctgaacac 
      gtccaggtc cacgaattg accatcaaa ccagttggg 
      gaaatgtac taa
    mRNA sequence
    • >TC015554 RA
      cagtctctg ggcgcaatg gtcgtgtct atggataga 
      tgggtgggc aaggtggct gtggtgacg ggcgctagt 
      acgggaatt ggggcggcg attgccaaa cagctcgtt 
      tccaatggg attattgtc gttggcatt gctcggaga 
      gttgaactc gtccaagag ctggcgcag gaactgggc 
      gacaggaaa ggtaaactc cacgcgtat aaagccgac 
      gtttcgaaa gaagacgac atcctcaag gccttcaaa 
      tggattgag gaaaatttg ggccccgta cacatacta 
      ataaacaat gcaggagtt gctaaagaa acaactcta 
      gtagatggc gataccgaa gcttggaga aacgtcctt 
      gacgtaaac gtccttggt ttatgtatt gcaacaaga 
      gaggcaatc aaaagcatg aaaaaacat gatataaaa 
      gggcatatc gttcacatt aacagcctg tgtgggcat 
      agagtgccc aattttcca ggcatgaat atttaccca 
      gcttctaaa cacgcagtc actgcactt acagaaact 
      ttaagacaa gaattgaat tttgttggc tccaaaatt 
      aaaatcacg agcgttagt cctggtcta gtaaccact 
      gaaatgaca actctcaat aaaaaccta gatcctgag 
      aagaaggca attgttgat aagtttcca gctctggag 
      actgaagat atcgcagat ggcgttatc tacgctttg 
      ggcacccct gaacacgtc caggtccac gaattgacc 
      atcaaacca gttggggaa atgtactaa gtaacaaat 
      aaaaaccat cacaacttt tctttttta tttattaac 
      tcattctac attatcgag catttcact gagtggcac 
      tttgtcgaa aaattttcc agctaaact gacggtaat 
      ttccgtgaa aaaactctc gtaaccact tttcacatc 
      attgaaaat cgcttgcca gatgtcgtt aagcacctt 
      attaatcat atcggaaaa ttcctggct acgattttc 
      ccgattttg gaaattcat gtaaagtag atcagtttt 
      gggaagtgt cacttttcc gtccacaat tctcaaatg 
      agtgagccc ttttttcgt gtatttttc cagcgggag 
      gaaggccac acctgtcac gttctctgg acaaaaata 
      gtatacaag acaatgaag atattgttc atctacaat 
      ctataccgt cgtgtataa gacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY