Status
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Gene information
General information
TC033362 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2056023..2066683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033362
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10183

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033362 RA
      MPVLSPRQS SASALTGSY RSTFSSSTI DRPYFGANY 
      SPRITTFSE RYTTRTYSD SDGRRIFSR SGSITEDGI 
      TSRFREEGR ESSIARRES LSRDSGISS IRENSIVDF 
      GRRDSSLSR DSPRLSSIN VLDSVAELR NKYSPANYV 
      PACLRERNN ENISRSKSI NDIGLPPIE TTKLAKKKV 
      INDSLNYNN SVLSNSNTP TNNDNNMTK VEEDDNGNR 
      ASVADLRKR FDEKNTKTT KSPLVKDLE ETSFKIAEN 
      RFKKFDSKV KTLKKNNDG GPKSSGRQL DVTSQTSSS 
      DCIINVSKL DVNETAAVK NGIRTHEDD GDCNEVRKT 
      TRVNNFASY IPAKDYQKN GDDSLNGDT EQGDESTTD 
      LIGKRFTDI STSPKLSAT STRSAISPS TSTVNSSSS 
      TSPSRRSSY NDDITTTAT TSPIKTQGL NGLKNIGNT 
      CFMNSVIQC LSNTKTLLE YLLQDHYVN DINTTTSAM 
      KGSLIKAFA EVIKKLWSQ DASDRVVNT SSLKSQIQR 
      FAPRFIGYA QQDAQEFLR YLLEGLHED VNRVKEKPK 
      PIITEIDEK LTDSEKSAE SWNRYLRMD NSKIVDYFV 
      GQLKSTLKC THCGHCSVT FDPFWDLSL PIPQRAGQV 
      RLAQCLDSF TREETLDGD EKP
    CDS sequence
    • >TC033362 RA
      atgcccgtt ttatcgccg agacagagc agtgcttcg 
      gcactgacg ggctcctac aggtccacg ttcagttcc 
      tcgaccatt gacaggccc tactttggg gccaattac 
      agcccccgg atcaccacc ttcagcgag aggtacacc 
      accaggacc tactcggac tcggacgga aggaggatt 
      ttttcgagg tctggcagc ataaccgaa gacggcata 
      accagccgt ttccgcgaa gaaggccgt gaatcgagc 
      atcgcccgt cgcgaatcc ctctcgcgc gattcgggc 
      ataagttca attcgcgaa aattcaatc gttgatttc 
      ggccgtcgt gacagttcc ctgtcccgg gacagcccc 
      cgtttatcc tcgatcaac gttctcgat tcggtcgcc 
      gaacttcgc aacaaatac tcaccggcc aactacgtt 
      cctgcatgt cttcgcgaa cgcaataac gagaacatc 
      tcacgttcg aaatccata aacgacatc ggcttaccg 
      ccgattgaa acaacaaaa ctcgccaaa aagaaggta 
      attaatgac agtttgaat tacaataac agcgtgttg 
      tccaattct aacacacct actaacaac gacaacaac 
      atgaccaaa gtggaagag gacgataat gggaatcgg 
      gcctcggtg gccgatttg cgcaaaaga ttcgacgag 
      aaaaataca aaaactaca aagtctccg ctggttaaa 
      gatttggaa gaaactagc tttaaaatc gccgagaat 
      cggttcaaa aaattcgat tctaaagtt aaaacttta 
      aaaaaaaat aacgatggt gggcctaaa tcaagtggg 
      cgccaattg gatgtgaca agtcaaacg tcaagttct 
      gattgtatt attaatgta tcgaaattg gatgtgaac 
      gagactgcg gcggtaaag aatggaatt cggacgcac 
      gaagatgat ggggattgt aacgaagtg agaaaaacg 
      acaagggtt aataatttt gcgtcgtat attccggcg 
      aaagattat caaaaaaac ggagacgat tctttaaac 
      ggggacacg gagcaaggg gacgaaagc acgacggat 
      ttgataggg aagaggttt acagatatc tcgacatcg 
      ccgaaactc agcgccacc tcgacccgt tcggcaatc 
      tccccctcc acctcgacc gtaaactcc tcgagttcg 
      acgagtcca tcgcgtcgt tcctcctac aacgatgac 
      atcaccaca acggcaaca acgagtcca ataaaaacg 
      caaggcctc aacggactc aaaaacatc ggcaacact 
      tgtttcatg aattccgtc attcagtgc ctctcgaac 
      acgaaaacc ctcttggag tacctcctc caggaccac 
      tacgtcaac gacatcaac accaccacc tcagcaatg 
      aagggttcc ctcattaag gcctttgcc gaagtcatc 
      aaaaaatta tggtcgcaa gacgcctcc gatcgtgtc 
      gtaaatacg tccagtctc aagagtcaa atacagcgt 
      tttgcgccc agatttatc ggttatgca caacaggac 
      gctcaggag tttttacgg tatttgctg gaggggttg 
      catgaggat gttaatcgg gtcaaagaa aagcccaaa 
      ccgatcatc accgaaatt gacgagaaa ttgacggat 
      agtgaaaag tcggccgag tcctggaat cgttacttg 
      cgaatggat aattcgaaa attgttgat tattttgtc 
      ggacagtta aaatcaacg ttgaaatgt acacattgt 
      ggccactgt tcggtcact tttgatcct ttttgggat 
      ttgtctttg ccgataccg cagagggct gggcaagtt 
      aggttggca caatgtttg gattctttc acgagggag 
      gaaacattg gatggggac gaaaaacca
    mRNA sequence
    • >TC033362 RA
      actcaagtc gctgacgtc atcgcaaga tggccgcca 
      cccaaaatg gactcattc agtttttta attttttag 
      caaaaattt gatacattt cggtaaatt tgcaacagt 
      ggggctatc acagtgccc gtgtgcgtg ttttggtgc 
      ggacgcagc cccaggact tcgcccccc ttggaccac 
      acacgagct aaggttaaa tgagcgatt gagtgataa 
      gggggtgat aagacaacg aaaatgccc gttttatcg 
      ccgagacag agcagtgct tcggcactg acgggctcc 
      tacaggtcc acgttcagt tcctcgacc attgacagg 
      ccctacttt ggggccaat tacagcccc cggatcacc 
      accttcagc gagaggtac accaccagg acctactcg 
      gactcggac ggaaggagg attttttcg aggtctggc 
      agcataacc gaagacggc ataaccagc cgtttccgc 
      gaagaaggc cgtgaatcg agcatcgcc cgtcgcgaa 
      tccctctcg cgcgattcg ggcataagt tcaattcgc 
      gaaaattca atcgttgat ttcggccgt cgtgacagt 
      tccctgtcc cgggacagc ccccgttta tcctcgatc 
      aacgttctc gattcggtc gccgaactt cgcaacaaa 
      tactcaccg gccaactac gttcctgca tgtcttcgc 
      gaacgcaat aacgagaac atctcacgt tcgaaatcc 
      ataaacgac atcggctta ccgccgatt gaaacaaca 
      aaactcgcc aaaaagaag gtaattaat gacagtttg 
      aattacaat aacagcgtg ttgtccaat tctaacaca 
      cctactaac aacgacaac aacatgacc aaagtggaa 
      gaggacgat aatgggaat cgggcctcg gtggccgat 
      ttgcgcaaa agattcgac gagaaaaat acaaaaact 
      acaaagtct ccgctggtt aaagatttg gaagaaact 
      agctttaaa atcgccgag aatcggttc aaaaaattc 
      gattctaaa gttaaaact ttaaaaaaa aataacgat 
      ggtgggcct aaatcaagt gggcgccaa ttggatgtg 
      acaagtcaa acgtcaagt tctgattgt attattaat 
      gtatcgaaa ttggatgtg aacgagact gcggcggta 
      aagaatgga attcggacg cacgaagat gatggggat 
      tgtaacgaa gtgagaaaa acgacaagg gttaataat 
      tttgcgtcg tatattccg gcgaaagat tatcaaaaa 
      aacggagac gattcttta aacggggac acggagcaa 
      ggggacgaa agcacgacg gatttgata gggaagagg 
      tttacagat atctcgaca tcgccgaaa ctcagcgcc 
      acctcgacc cgttcggca atctccccc tccacctcg 
      accgtaaac tcctcgagt tcgacgagt ccatcgcgt 
      cgttcctcc tacaacgat gacatcacc acaacggca 
      acaacgagt ccaataaaa acgcaaggc ctcaacgga 
      ctcaaaaac atcggcaac acttgtttc atgaattcc 
      gtcattcag tgcctctcg aacacgaaa accctcttg 
      gagtacctc ctccaggac cactacgtc aacgacatc 
      aacaccacc acctcagca atgaagggt tccctcatt 
      aaggccttt gccgaagtc atcaaaaaa ttatggtcg 
      caagacgcc tccgatcgt gtcgtaaat acgtccagt 
      ctcaagagt caaatacag cgttttgcg cccagattt 
      atcggttat gcacaacag gacgctcag gagttttta 
      cggtatttg ctggagggg ttgcatgag gatgttaat 
      cgggtcaaa gaaaagccc aaaccgatc atcaccgaa 
      attgacgag aaattgacg gatagtgaa aagtcggcc 
      gagtcctgg aatcgttac ttgcgaatg gataattcg 
      aaaattgtt gattatttt gtcggacag ttaaaatca 
      acgttgaaa tgtacacat tgtggccac tgttcggtc 
      acttttgat cctttttgg gatttgtct ttgccgata 
      ccgcagagg gctgggcaa gttaggttg gcacaatgt 
      ttggattct ttcacgagg gaggaaaca ttggatggg 
      gacgaaaaa ccatgaaat tttctgaga gagaatgaa 
      aaaaatacc gaaattact atttatttc ggtctaatt 
      tactgtttt ttcggtttg atatgaata aaattttgt 
      gaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY