Status
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Gene information
General information
TC015164 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2131536..2141848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015164
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10182

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015164 RA
      MSQQHDDRK KFYCRKWAF QKLSHCLDQ RPVSKTCGA 
      LLLGGSGCG KTTFCSELC WPSQGPSGR QQRALNRRL 
      LCYHFLQGH NEKSLSLSE FVRSLVTQI LNHSTESHK 
      ERSMRRKLT QEKTDSEHV SELYSETVS PTLEKKNEV 
      VLPVNPRTL IADAYLDKL KSDPEIQIA LRAKNIELN 
      PDDCLKRAL LFPLLEIDP PKTSLFILI DSIDEHSFV 
      YSNSTLTRS KPRERSSQS KTIAELLAN HHHLFPQWL 
      LLVCTARKQ SKSIAKMFT GFRKLSLDD LRKSQVVRD 
      VQQYILARL DQENSLRQH ISRDTAEML NQLHIKSNG 
      CFLYLEKVL DGVSDNFIV LREVREIPG TLNGLYLWL 
      CQRLFNRRQ FAKVQQLLN VILAAKCAL NEALLFDII 
      KVYNPKMTQ DDFKKRFNL LRRIIVVSK SGAIRLFHH 
      SFTEWLLDI KHCTQKYLC NIFHGHCIL AMYYTLHAK 
      YLNNQEIYD YAFHLTRME QYLNEKPTQ KCPTLMYKL 
      SQEKHQGEN SLEKTSTEI YADLKALQE LTKFEEKEV 
      TGALETSFI ETENSLDEI TDKFDNVNL KSPTVDKNP 
      IYAEVNKIK KTEMVKTDD PSTVSSTLE PNKTILDVH 
      TLTVLWLIL SGCNVEECL LEGNEMAGV NFGAFLPTD 
      PKVLKLLLE AGAVEQPDV EGCEYESQM SLASTSSEQ 
      SPEPLFDLH DLHGQAALH VAARLGQAQ VVKVLLEAG 
      ANADQADVD GWTPLRAAA WGGHTEVVE LLVEHGCAL 
      DSVDAENRT ALRAAAWSG HEEIVKILL QHGANVNLT 
      DHEGRTALI AAAYMGHSE IVEHLLDYG ADVNHADAD 
      GRTALSVAA LCAPRTPGV NVVSTLLER GATVDHRDK 
      EGMTPLLVA SFEGHKDVC ELLLENEAD VDHCDHSGR 
      SPLWAAASM GHAPVVALL LFWGCCIDS MDGEGRTVL 
      SVAAAQGCV EVVRQLLDR GLDEQHRDN SGWTPLHYA 
      AFEGHQDVC EALLEAGAR IDETDNEGK APLALAAQG 
      GHAALVSMF LDKYNAPID QRPHDGKTA LRLAALEGH 
      YEVVQLLTT HGADIDSKD ADGRSTLYV LALDNRLAM 
      SKYFIQQRA DVETRDLEG RTPLHVSSW QGHTEMVSL 
      LLTYGKCQV DACDLENRT ALHSASWQG HSDIVKLLL 
      EHGALPDHT CNQGATALG IAAQEGHEL CVVALLEHG 
      ADPNHSDAC GRNALRVAA KSGHRGVVR LLEEYNARS 
      HKMAHTSSS ANSITSAST AETKPSCAV LYPGNQDWC 
      DQQRRSMVS LGNHSSNSK SSSNLTGSS HSSRHGDRQ 
      RDNSQNSQP MSFTQQLQQ CTRGGKTRP LSKLLSPLQ 
      SEPQSPIYA SPPLSPVHD TPNMFGAMN IYQPVLRHN 
      NFAKAPDTH FARDTHMRI ILGNSSGIG VGNKKGDQQ 
      NNESNSKSS SQKPKRNGI VTNPALRLV ANVKNGLDT 
      AAANLRKST SGANPSTKT NSFHWRKET PL
    CDS sequence
    • >TC015164 RA
      atgtcgcaa caacacgac gaccgcaaa aaattctat 
      tgccgcaaa tgggccttc caaaagcta tcacattgc 
      ctcgaccaa cgtcccgtc tccaaaaca tgtggcgcc 
      cttctcctc ggcggctcc ggttgcggc aaaacgacc 
      ttttgctcc gaactgtgt tggccatcg cagggcccc 
      agcggccgc caacaacgc gccctcaac cgccgcctc 
      ctctgctac cacttcctc caaggccac aacgagaaa 
      agcctctcc ttgagcgaa ttcgtccgc tccttagtc 
      acccaaatc ctgaaccat tcgaccgaa agccacaaa 
      gagcgcagc atgcgccgg aaattgacc caagaaaag 
      actgactct gaacacgtg agtgaactg tacagtgag 
      acagtgtca ccaactttg gagaaaaaa aatgaggtg 
      gtgctaccg gtgaatccc cgcacgttg attgctgat 
      gcgtatttg gataagttg aagtccgac cctgagata 
      caaatcgcc ctaagggcc aaaaatata gaattgaac 
      cctgatgat tgtctcaag agggcgttg ctgttcccc 
      ctcctggag atcgacccg cccaaaacc tcgctcttt 
      attctgatt gattcgatc gacgagcac tcgttcgtc 
      tactccaat tcgacgctg acgcgttcg aagccacgt 
      gagcgcagc tcccaatca aaaacgatc gccgagttg 
      ctggccaat caccaccac ctgttcccc cagtggctc 
      ctcctcgtg tgcaccgcc cgcaagcaa tcgaaaagc 
      atagcgaaa atgttcacc ggcttccgt aaactgagt 
      ctggacgat ttgcgcaaa tcgcaagtt gtgcgcgac 
      gtccaacag tacattctg gcaagactg gaccaggag 
      aacagtctg cgtcaacat ataagtcgc gacacggcc 
      gaaatgctc aaccaattg cacatcaaa agcaacggc 
      tgctttctc tacttggag aaggttctg gacggcgtt 
      tcggacaat ttcatcgtt ctgcgggaa gttcgcgag 
      attcccggc acgctaaac gggctctat ttgtggctg 
      tgtcagaga ctcttcaac cggagacag ttcgccaaa 
      gtccagcag ttgctgaat gtgatcctt gcggcgaag 
      tgtgcgctc aacgaggcg ctgctgttc gacatcatc 
      aaagtgtac aacccgaaa atgacacag gatgacttc 
      aagaagcgg tttaatctc ttaaggcgg attatcgtg 
      gtgtcgaag tcgggcgcg attcgcttg tttcaccat 
      tcgttcacc gagtggttg ctggatatt aaacactgc 
      actcagaag tacttgtgt aatattttt cacgggcat 
      tgcatcctt gccatgtac tacacgctg cacgccaag 
      tatttgaac aatcaggag atctacgat tatgcgttt 
      catttgacg cggatggag cagtatttg aacgaaaaa 
      ccgacacag aaatgccca acgttgatg tataagttg 
      tcgcaagag aagcaccaa ggggagaac tcgctggag 
      aagacgtcc acggagatt tacgccgat ttgaaggcc 
      ttgcaggag ctgaccaag tttgaggag aaggaagtg 
      acaggggct cttgagacc agctttatc gaaacggag 
      aacagcctc gacgaaatc accgataaa ttcgacaac 
      gtcaacctc aagagcccc accgtggac aagaacccc 
      atctatgcg gaagtgaac aaaatcaaa aaaaccgaa 
      atggtaaaa accgacgac ccatcaacc gtcagcagc 
      accctcgag cccaacaaa accatcctt gacgtgcac 
      actttgacc gtactttgg ctcattttg agcggttgt 
      aacgtcgaa gagtgcctt ctggagggc aacgaaatg 
      gccggagtc aactttggg gccttccta ccaaccgac 
      cccaaagtc ctgaaattg ctgctagag gcaggcgct 
      gttgagcag ccagacgtt gaaggatgc gaatatgaa 
      agtcaaatg tctttggcg tcgacgtcg agtgaacaa 
      agtccagaa ccgctgttt gatctgcac gatctgcac 
      ggacaagct gccctgcat gtggcagcc aggctgggg 
      caggcgcag gtcgttaag gtgttgttg gaggcgggc 
      gccaacgcc gaccaggcc gacgtggac ggctggacc 
      cctctgcgc gccgccgcc tggggcggc cacaccgaa 
      gtggtcgaa ctcctggtg gagcacggc tgcgccttg 
      gacagcgtc gacgccgaa aatcgcacc gctttacgt 
      gcggctgcc tggtccggc cacgaggaa atcgtcaag 
      atcctcctc caacacggc gccaacgtc aacctaacc 
      gaccacgag ggccggacg gccctcatc gccgccgcc 
      tacatgggc cacagcgaa atcgtcgaa cacctcctt 
      gactacggc gccgacgtc aatcacgcc gacgccgac 
      ggccgaacg gccctgtcc gtagccgcg ttatgcgcc 
      cctcggacg ccgggcgtc aacgtcgtc tcgacgctt 
      ctagagcgc ggcgccacc gtcgaccac cgcgacaaa 
      gagggcatg acgccccta ttggtcgcc tcgttcgaa 
      ggccacaaa gacgtgtgc gagctgcta ttggagaac 
      gaagccgac gtggatcat tgcgaccac agtgggcgg 
      agtccctta tgggcggcg gcgagtatg ggccacgcc 
      cccgtggtg gcgcttttg ttgttttgg gggtgttgt 
      atcgattcg atggatggg gaaggtcgg acggttttg 
      tcagtggcg gcggcacaa gggtgtgtc gaagttgtg 
      agacagttg ttggatcgg gggttggat gaacaacat 
      cgggataat tccgggtgg acgccactg cattatgcg 
      gcgtttgaa gggcatcag gatgtttgt gaggcgttg 
      ctggaggcg ggggcacgg attgatgag actgataat 
      gagggcaaa gcaccgctt gccttagcg gcacaaggg 
      gggcacgca gctttagtt agtatgttt ttagataag 
      tataacgcg ccgattgat cagaggcca catgatggc 
      aaaactgcc ttaaggttg gcagcactg gagggtcat 
      tatgaagtt gtacagttg ttgacgact cacggggct 
      gatattgac tctaaagat gcggacggt aggagcact 
      ttatatgtt ttagcactg gataatcgg ttagccatg 
      tccaaatat tttattcaa caaagggct gatgttgag 
      acacgtgac ttggagggt cggacgccc cttcacgtc 
      tcctcctgg caaggccac actgaaatg gtctccctg 
      ctcctcacc tatggcaag tgtcaagtg gacgcttgc 
      gaccttgaa aatcgcacg gccttgcat tcggccagt 
      tggcaaggt cacagcgac attgttaag ctactcctt 
      gaacatgga gccttacca gaccacaca tgcaatcaa 
      ggagccaca gctttgggc atcgccgcc caagaaggc 
      catgaattg tgtgtggtg gcgctactg gagcacggc 
      gccgaccca aaccactct gatgcttgc ggacgcaac 
      gccttaaga gtggccgcg aaatcaggc caccgtggt 
      gtggtgaga ctcttggag gagtacaac gccaggtca 
      cacaaaatg gcacatacg agcagtagt gccaactca 
      ataacatca gcttcgaca gctgagact aaaccatcg 
      tgtgctgtg ctatatccc ggaaaccag gactggtgt 
      gaccagcag agacgctca atggtgtcg ctggggaat 
      cacagttca aattcgaaa tcgagctcg aacttgact 
      gggtccagt cactcgagt cggcatggc gaccgccag 
      agggacaac tcgcaaaac tcacaacct atgtctttc 
      acccaacag ttgcaacaa tgcacaagg ggtggcaaa 
      acgagaccg ttgagtaaa ttactatcg ccgctgcaa 
      agcgagcca cagagtccc atctacgcg tcgccgccg 
      ctcagtccc gttcacgac acgcctaac atgttcggc 
      gctatgaac atctaccag ccggtgctg cggcataac 
      aacttcgcc aaggcgccc gacacgcat ttcgcgcgc 
      gacacccac atgcgcatc attctgggt aactcgagc 
      ggtatcggc gtgggaaac aagaagggt gaccagcag 
      aacaatgag tcgaattcg aaatctagc agtcagaag 
      ccgaaacgt aacggtatt gtgacgaat ccagcgctg 
      cgattggtg gcgaatgtg aagaatggg ctggacacg 
      gccgccgcc aacttgagg aaatcaacg tccggcgcg 
      aatccgagt acaaaaacg aacagtttt cattggagg 
      aaagaaacg ccattgtaa
    mRNA sequence
    • >TC015164 RA
      ccaggcgtt aacctcagg tagcccccc tgcaatggc 
      cccccaatg gcaataaac ccccccaca ccatcaccc 
      cgccccagg tgatcgcaa taatgtagc caaaatgtc 
      gcaacaaca cgacgaccg caaaaaatt ctattgccg 
      caaatgggc cttccaaaa gctatcaca ttgcctcga 
      ccaacgtcc cgtctccaa aacatgtgg cgcccttct 
      cctcggcgg ctccggttg cggcaaaac gaccttttg 
      ctccgaact gtgttggcc atcgcaggg ccccagcgg 
      ccgccaaca acgcgccct caaccgccg cctcctctg 
      ctaccactt cctccaagg ccacaacga gaaaagcct 
      ctccttgag cgaattcgt ccgctcctt agtcaccca 
      aatcctgaa ccattcgac cgaaagcca caaagagcg 
      cagcatgcg ccggaaatt gacccaaga aaagactga 
      ctctgaaca cgtgagtga actgtacag tgagacagt 
      gtcaccaac tttggagaa aaaaaatga ggtggtgct 
      accggtgaa tccccgcac gttgattgc tgatgcgta 
      tttggataa gttgaagtc cgaccctga gatacaaat 
      cgccctaag ggccaaaaa tatagaatt gaaccctga 
      tgattgtct caagagggc gttgctgtt ccccctcct 
      ggagatcga cccgcccaa aacctcgct ctttattct 
      gattgattc gatcgacga gcactcgtt cgtctactc 
      caattcgac gctgacgcg ttcgaagcc acgtgagcg 
      cagctccca atcaaaaac gatcgccga gttgctggc 
      caatcacca ccacctgtt cccccagtg gctcctcct 
      cgtgtgcac cgcccgcaa gcaatcgaa aagcatagc 
      gaaaatgtt caccggctt ccgtaaact gagtctgga 
      cgatttgcg caaatcgca agttgtgcg cgacgtcca 
      acagtacat tctggcaag actggacca ggagaacag 
      tctgcgtca acatataag tcgcgacac ggccgaaat 
      gctcaacca attgcacat caaaagcaa cggctgctt 
      tctctactt ggagaaggt tctggacgg cgtttcgga 
      caatttcat cgttctgcg ggaagttcg cgagattcc 
      cggcacgct aaacgggct ctatttgtg gctgtgtca 
      gagactctt caaccggag acagttcgc caaagtcca 
      gcagttgct gaatgtgat ccttgcggc gaagtgtgc 
      gctcaacga ggcgctgct gttcgacat catcaaagt 
      gtacaaccc gaaaatgac acaggatga cttcaagaa 
      gcggtttaa tctcttaag gcggattat cgtggtgtc 
      gaagtcggg cgcgattcg cttgtttca ccattcgtt 
      caccgagtg gttgctgga tattaaaca ctgcactca 
      gaagtactt gtgtaatat ttttcacgg gcattgcat 
      ccttgccat gtactacac gctgcacgc caagtattt 
      gaacaatca ggagatcta cgattatgc gtttcattt 
      gacgcggat ggagcagta tttgaacga aaaaccgac 
      acagaaatg cccaacgtt gatgtataa gttgtcgca 
      agagaagca ccaagggga gaactcgct ggagaagac 
      gtccacgga gatttacgc cgatttgaa ggccttgca 
      ggagctgac caagtttga ggagaagga agtgacagg 
      ggctcttga gaccagctt tatcgaaac ggagaacag 
      cctcgacga aatcaccga taaattcga caacgtcaa 
      cctcaagag ccccaccgt ggacaagaa ccccatcta 
      tgcggaagt gaacaaaat caaaaaaac cgaaatggt 
      aaaaaccga cgacccatc aaccgtcag cagcaccct 
      cgagcccaa caaaaccat ccttgacgt gcacacttt 
      gaccgtact ttggctcat tttgagcgg ttgtaacgt 
      cgaagagtg ccttctgga gggcaacga aatggccgg 
      agtcaactt tggggcctt cctaccaac cgaccccaa 
      agtcctgaa attgctgct agaggcagg cgctgttga 
      gcagccaga cgttgaagg atgcgaata tgaaagtca 
      aatgtcttt ggcgtcgac gtcgagtga acaaagtcc 
      agaaccgct gtttgatct gcacgatct gcacggaca 
      agctgccct gcatgtggc agccaggct ggggcaggc 
      gcaggtcgt taaggtgtt gttggaggc gggcgccaa 
      cgccgacca ggccgacgt ggacggctg gacccctct 
      gcgcgccgc cgcctgggg cggccacac cgaagtggt 
      cgaactcct ggtggagca cggctgcgc cttggacag 
      cgtcgacgc cgaaaatcg caccgcttt acgtgcggc 
      tgcctggtc cggccacga ggaaatcgt caagatcct 
      cctccaaca cggcgccaa cgtcaacct aaccgacca 
      cgagggccg gacggccct catcgccgc cgcctacat 
      gggccacag cgaaatcgt cgaacacct ccttgacta 
      cggcgccga cgtcaatca cgccgacgc cgacggccg 
      aacggccct gtccgtagc cgcgttatg cgcccctcg 
      gacgccggg cgtcaacgt cgtctcgac gcttctaga 
      gcgcggcgc caccgtcga ccaccgcga caaagaggg 
      catgacgcc cctattggt cgcctcgtt cgaaggcca 
      caaagacgt gtgcgagct gctattgga gaacgaagc 
      cgacgtgga tcattgcga ccacagtgg gcggagtcc 
      cttatgggc ggcggcgag tatgggcca cgcccccgt 
      ggtggcgct tttgttgtt ttgggggtg ttgtatcga 
      ttcgatgga tggggaagg tcggacggt tttgtcagt 
      ggcggcggc acaagggtg tgtcgaagt tgtgagaca 
      gttgttgga tcgggggtt ggatgaaca acatcggga 
      taattccgg gtggacgcc actgcatta tgcggcgtt 
      tgaagggca tcaggatgt ttgtgaggc gttgctgga 
      ggcgggggc acggattga tgagactga taatgaggg 
      caaagcacc gcttgcctt agcggcaca aggggggca 
      cgcagcttt agttagtat gtttttaga taagtataa 
      cgcgccgat tgatcagag gccacatga tggcaaaac 
      tgccttaag gttggcagc actggaggg tcattatga 
      agttgtaca gttgttgac gactcacgg ggctgatat 
      tgactctaa agatgcgga cggtaggag cactttata 
      tgttttagc actggataa tcggttagc catgtccaa 
      atattttat tcaacaaag ggctgatgt tgagacacg 
      tgacttgga gggtcggac gccccttca cgtctcctc 
      ctggcaagg ccacactga aatggtctc cctgctcct 
      cacctatgg caagtgtca agtggacgc ttgcgacct 
      tgaaaatcg cacggcctt gcattcggc cagttggca 
      aggtcacag cgacattgt taagctact ccttgaaca 
      tggagcctt accagacca cacatgcaa tcaaggagc 
      cacagcttt gggcatcgc cgcccaaga aggccatga 
      attgtgtgt ggtggcgct actggagca cggcgccga 
      cccaaacca ctctgatgc ttgcggacg caacgcctt 
      aagagtggc cgcgaaatc aggccaccg tggtgtggt 
      gagactctt ggaggagta caacgccag gtcacacaa 
      aatggcaca tacgagcag tagtgccaa ctcaataac 
      atcagcttc gacagctga gactaaacc atcgtgtgc 
      tgtgctata tcccggaaa ccaggactg gtgtgacca 
      gcagagacg ctcaatggt gtcgctggg gaatcacag 
      ttcaaattc gaaatcgag ctcgaactt gactgggtc 
      cagtcactc gagtcggca tggcgaccg ccagaggga 
      caactcgca aaactcaca acctatgtc tttcaccca 
      acagttgca acaatgcac aaggggtgg caaaacgag 
      accgttgag taaattact atcgccgct gcaaagcga 
      gccacagag tcccatcta cgcgtcgcc gccgctcag 
      tcccgttca cgacacgcc taacatgtt cggcgctat 
      gaacatcta ccagccggt gctgcggca taacaactt 
      cgccaaggc gcccgacac gcatttcgc gcgcgacac 
      ccacatgcg catcattct gggtaactc gagcggtat 
      cggcgtggg aaacaagaa gggtgacca gcagaacaa 
      tgagtcgaa ttcgaaatc tagcagtca gaagccgaa 
      acgtaacgg tattgtgac gaatccagc gctgcgatt 
      ggtggcgaa tgtgaagaa tgggctgga cacggccgc 
      cgccaactt gaggaaatc aacgtccgg cgcgaatcc 
      gagtacaaa aacgaacag ttttcattg gaggaaaga 
      aacgccatt gtaaaatta attgttaag tcactgcgt 
      gaattacta gtcaatact atacggtgg ggatttttt 
      cgtttgaat gttgccctg acaggtttt taaaacgct 
      aacaacact cacctagtc ttacctata tcaaagatt 
      taatttttt attagatta tcgaatggt taatctagt 
      agcgacata actcagatt ctaggccta agtttgttc 
      aacgatgag tttactgaa atcagttgt gatagagac 
      gtagtttag taaacaggt aacaataat tcaaggctt 
      cagttcagt tctctctcg taagatctc attttgcaa 
      aatatattt ttgtatgtt tggagttat tttcgggta 
      ttgtgtgtg tttctcttc aagcaattc gctgtttca 
      ttcaatacg tatcaatta atgtatgcc ttttttgta 
      tttacaata aaacagctt tagttttat actattaaa 
      gttgtgcac tgtatatgt tcagaacag tattaaact 
      tgaaatatt taaataaat acatgtaaa agtg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY