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Gene information
General information
TC015531 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 2006764..2008249
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015531
    iBeetle screen iB_10175

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: There is something whitish stuff inside the empty eggs.
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015531 RA
      MHYLFVLGL VFAPVLPKS LPTNIRRCS KSDPNFNKC 
      LQESAQDAT LKLAQGNKD LGVVPLEPF FIKKVDLGN 
      NTAGAVTVH QVYENLNIY GMTNGTITY SEANFTGNN 
      CYWNYKVRG TLIRMVSDY TMTGQLLIF PINGHGKCT 
      NYLYDYDGD YEAKCERYN KKNKTYLRV TDFKFGFKP 
      KRIVFDFEN IIDGNEQLS KEVVKTLNE NSETVYADV 
      GPAFNQVMG MVWKQTLNQ VFLRVPEDE LFLP
    CDS sequence
    • >TC015531 RA
      atgcactat ttattcgtt ttgggccta gtttttgcc 
      ccagttttg ccaaaatcg ttgcctaca aatattcgc 
      agatgtagt aagtccgac ccaaatttt aataaatgt 
      ttgcaagaa agtgcccaa gatgccact cttaaactg 
      gcccaaggc aacaaggat ttgggggtt gtgcctttg 
      gagccgttt tttatcaaa aaagttgat ttagggaat 
      aatacagcg ggggctgtt actgtgcac caggtgtac 
      gaaaatttg aacatttac ggaatgact aatgggacc 
      attacttat tcggaggct aattttacg ggaaataat 
      tgttactgg aattataaa gttcgaggg actttgatt 
      agaatggtc tctgattat acaatgacg ggccagttg 
      ttgattttt cccatcaat ggacacgga aaatgcacc 
      aattactta tacgattat gacggtgat tacgaggcg 
      aaatgcgag cgctacaac aagaaaaat aaaacttat 
      ctaagagtg accgatttt aagttcggt ttcaagcca 
      aagagaatt gttttcgat tttgagaat atcatagat 
      gggaatgag caattaagc aaggaagtg gttaagaca 
      cttaatgaa aattcggaa acggtttat gccgatgtg 
      gggccggct tttaaccaa gtcatggga atggtttgg 
      aagcaaacg ctgaatcaa gtgtttctg agagtgcca 
      gaagatgaa cttttctta ccttga
    mRNA sequence
    • >TC015531 RA
      gcgcgaaaa gtccggcaa gcgcgaaac tggcgccat 
      tagcgcact tattcgaac ttttaggac ttaattttt 
      tatttcgta aaatgttgt tttaaaata cgaaatgtt 
      tgccgtttt tgaccactc aactgatca attttatcg 
      gccattgcg aaaaacgca ccaaatgac gcaacttct 
      tcaaggttt caaaacact ttcattttg ttcgtttgc 
      tcttaaacc ggtttttat tattttgta aaattgtaa 
      acactttaa aatgcacta tttattcgt tttgggcct 
      agtttttgc cccagtttt gccaaaatc gttgcctac 
      aaatattcg cagatgtag taagtccga cccaaattt 
      taataaatg tttgcaaga aagtgccca agatgccac 
      tcttaaact ggcccaagg caacaagga tttgggggt 
      tgtgccttt ggagccgtt ttttatcaa aaaagttga 
      tttagggaa taatacagc gggggctgt tactgtgca 
      ccaggtgta cgaaaattt gaacattta cggaatgac 
      taatgggac cattactta ttcggaggc taattttac 
      gggaaataa ttgttactg gaattataa agttcgagg 
      gactttgat tagaatggt ctctgatta tacaatgac 
      gggccagtt gttgatttt tcccatcaa tggacacgg 
      aaaatgcac caattactt atacgatta tgacggtga 
      ttacgaggc gaaatgcga gcgctacaa caagaaaaa 
      taaaactta tctaagagt gaccgattt taagttcgg 
      tttcaagcc aaagagaat tgttttcga ttttgagaa 
      tatcataga tgggaatga gcaattaag caaggaagt 
      ggttaagac acttaatga aaattcgga aacggttta 
      tgccgatgt ggggccggc ttttaacca agtcatggg 
      aatggtttg gaagcaaac gctgaatca agtgtttct 
      gagagtgcc agaagatga acttttctt accttgaaa 
      ccataaaaa ttgtgttta tgtatggtg ttgtaaaat 
      ttgcgtaat tgaataaaa atgcggtgg tttatggct 
      gttttaatg gtttgtagg ttcaagagt cgcgtaatt 
      aatgtttaa attggaata aaatgtttg tgtgtaggc 
      aaccaacaa tgcacgcca ttgttttta aacattttt 
      ttaataaaa attctttta attt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY