Status
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Gene information
General information
TC015193 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1671503..1675857
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015193
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10174

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015193 RA
      MASAAAAKI AEVRETTRV ERIGAHSHI RGLGLDESL 
      EARHVSQGM VGQVSARRA IGIVLKMVR EGRIAGRAV 
      LLAGQPGTG KTAIATALA AALGQDTPF TSMAGSEIY 
      SLEMSKTEA LTQAIRKSI GVRIKEESE IIEGEVVEV 
      QIERPATGV GTKVGKLIL KTTEMETVY DLGGKMIDS 
      LLKEKVQSG DVITIDKAT GKISRLGRS FARARDYDA 
      TGQQTRFVQ CPEGELQKR KEVVHTVTL HEIDVINSR 
      THGFLALFS GDTGEIKPE VREQINGKV AEWREEGKA 
      EMIPGVLFI DEVHMLDIE CFSFLNRAL ENEMAPIVI 
      MATNRGITR IRGTNYKSP HGIPLDLLD RMIIVPTAP 
      YDEKELREI LSIRCEEED CQMSDNALT VLTRISKET 
      SLRYGMQLI MTSSLIARK RKAPEVDVE DIKRAYQLF 
      FDEGRSVQF LKEYQQEFM FNEDDAIEM DTT
    CDS sequence
    • >TC015193 RA
      atggctagc gccgccgct gcaaaaatc gccgaagtg 
      cgtgaaacc acgcgcgtg gagcgcatt ggggcccac 
      tcccacatc cgggggctg ggtctggat gagagtctc 
      gaggcccgg catgtctcg caagggatg gtggggcag 
      gtgtctgcc aggagggct atcgggatt gtcttgaag 
      atggtgagg gaggggcgg attgctggt cgggccgtg 
      cttttggcg gggcaaccg gggacgggg aaaacggcc 
      attgcgacg gctctggcc gccgccctg gggcaggac 
      acccccttt acgagcatg gcaggctcg gagatttac 
      tcgctggag atgagcaag acggaggcg ctgacgcag 
      gcgattagg aagagtatc ggggtgcgg atcaaggag 
      gagagcgaa ataatcgaa ggtgaagtc gtcgaagta 
      caaatcgaa cgtcctgcc accggcgtt ggtacaaaa 
      gtaggtaaa ctaatcctc aaaacaacg gaaatggaa 
      accgtctac gacctcggt ggcaaaatg atcgattcg 
      ttattgaaa gagaaggtt caatcaggc gacgtgata 
      acaattgac aaagccacc ggcaaaata tcgcgtctt 
      ggacgctct ttcgcacgt gcccgagac tacgacgcc 
      actggccag caaactcgt ttcgttcaa tgtcccgaa 
      ggcgaactc caaaagcgg aaggaagtc gttcacaca 
      gtgaccttg cacgaaatc gacgtcatc aacagtcgc 
      acgcacggc ttcctcgcc cttttttcg ggcgacacc 
      ggcgaaatc aagccggaa gttcgcgag caaatcaac 
      ggaaaagtg gccgaatgg cgcgaggag ggcaaggcg 
      gaaatgatc cccggggtg cttttcatc gacgaggtc 
      cacatgctc gatatcgag tgtttctcg tttttgaat 
      cgcgcgctc gaaaacgaa atggcgccg attgtcatc 
      atggcgacc aatcgggga atcacacgc atccgtggc 
      accaattac aaatcgccg catgggatt cccctcgat 
      ttgctcgat cgcatgatc atcgtgccc acggcaccc 
      tacgatgag aaggagttg cgcgagatt ttgagcatc 
      cgttgcgag gaggaagac tgtcaaatg tctgacaac 
      gcactgact gttttgaca cgaatttcg aaagaaacg 
      tcactgcgc tacggcatg cagttgatc atgacgtca 
      agtttaatt gcgcgcaag cgcaaagcc cccgaagtc 
      gatgttgag gacatcaag agggcgtat caattgttt 
      ttcgatgaa gggcgctcg gttcaattt ctcaaggag 
      taccaacag gagtttatg ttcaatgaa gatgacgcg 
      atcgaaatg gacacgact taa
    mRNA sequence
    • >TC015193 RA
      ccgcgtccc tccattgtt tttgcattt cctttaaaa 
      ttgtgcata aaaacgcaa aaaagccgc aaaaaccac 
      aaccccaat ggctagcgc cgccgctgc aaaaatcgc 
      cgaagtgcg tgaaaccac gcgcgtgga gcgcattgg 
      ggcccactc ccacatccg ggggctggg tctggatga 
      gagtctcga ggcccggca tgtctcgca agggatggt 
      ggggcaggt gtctgccag gagggctat cgggattgt 
      cttgaagat ggtgaggga ggggcggat tgctggtcg 
      ggccgtgct tttggcggg gcaaccggg gacggggaa 
      aacggccat tgcgacggc tctggccgc cgccctggg 
      gcaggacac cccctttac gagcatggc aggctcgga 
      gatttactc gctggagat gagcaagac ggaggcgct 
      gacgcaggc gattaggaa gagtatcgg ggtgcggat 
      caaggagga gagcgaaat aatcgaagg tgaagtcgt 
      cgaagtaca aatcgaacg tcctgccac cggcgttgg 
      tacaaaagt aggtaaact aatcctcaa aacaacgga 
      aatggaaac cgtctacga cctcggtgg caaaatgat 
      cgattcgtt attgaaaga gaaggttca atcaggcga 
      cgtgataac aattgacaa agccaccgg caaaatatc 
      gcgtcttgg acgctcttt cgcacgtgc ccgagacta 
      cgacgccac tggccagca aactcgttt cgttcaatg 
      tcccgaagg cgaactcca aaagcggaa ggaagtcgt 
      tcacacagt gaccttgca cgaaatcga cgtcatcaa 
      cagtcgcac gcacggctt cctcgccct tttttcggg 
      cgacaccgg cgaaatcaa gccggaagt tcgcgagca 
      aatcaacgg aaaagtggc cgaatggcg cgaggaggg 
      caaggcgga aatgatccc cggggtgct tttcatcga 
      cgaggtcca catgctcga tatcgagtg tttctcgtt 
      tttgaatcg cgcgctcga aaacgaaat ggcgccgat 
      tgtcatcat ggcgaccaa tcggggaat cacacgcat 
      ccgtggcac caattacaa atcgccgca tgggattcc 
      cctcgattt gctcgatcg catgatcat cgtgcccac 
      ggcacccta cgatgagaa ggagttgcg cgagatttt 
      gagcatccg ttgcgagga ggaagactg tcaaatgtc 
      tgacaacgc actgactgt tttgacacg aatttcgaa 
      agaaacgtc actgcgcta cggcatgca gttgatcat 
      gacgtcaag tttaattgc gcgcaagcg caaagcccc 
      cgaagtcga tgttgagga catcaagag ggcgtatca 
      attgttttt cgatgaagg gcgctcggt tcaatttct 
      caaggagta ccaacagga gtttatgtt caatgaaga 
      tgacgcgat cgaaatgga cacgactta atcgcgtta 
      cctttgcct tgtgacact tgacccggg aattttatt 
      gacagttgg atttcccgc cattttttt cggtttttt 
      tttttttag ttaattgca tcgcgttct agatcgggt 
      gaatggtga aatattcag gtgtgtgtt gagaaattt 
      ccatgatgt tgtttcatg cgaaataat tgggggaaa 
      cggcaattt cgagtcaaa tttttgacg tttccttaa 
      tcttctcga atcaaagtg tcacacttg acatgttta 
      cataattcc gttattatg taagtttgt gtatttttt 
      gaataaaat atttaatta aca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY