Status
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Gene information
General information
TC015511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1643019..1645471
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015511
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10171

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015511 RA
      MGNHHGHGP HSKSGDSTP TSNGSRKSI SRTVSNHEI 
      QADKPSALL PVEKLTKIL ADKSFEEEH ITGISLNVF 
      TKCLFPRYP VLAEKLYTY LLTGAKSRN TYIDIQGFK 
      LQCEKYLSI LDDETVRDT FVRMFGTQV EDAGEVITP 
      EGLRSLLMC AYHVSMDHY SEGPQMCLS ISKTLKAVV 
      DSCFHTKTQ LSTQFVSHW LAANCPRLL LPLHRYLVH 
      SLATSWRTL EDMENAPAA GLELATPVL EQAPPFSQK 
      HPHLLHMSM SWLLAGALP PVFSRPQKA HSPSNSGVG 
      LASTAFLTK LLCSVPSHW VVLYDSDND GLGANRFLH 
      HVMSYKGPT LCLLRVEDG QVFCIASPN EWRESNHYW 
      GGEDSAVFQ LLPKFVLLE KGSKMLYLN TTVRGYPYG 
      LRAGKDPRS PIIIVDGGF EKMEFKKIP YSLLRIEVW 
      GCGDPISRE QQLEVKKWE VKEAERQRC VKLSADDWL 
      DHPDRYLLE LAGRPQYHQ NQTQ
    CDS sequence
    • >TC015511 RA
      atggggaac caccacgga cacggcccc catagcaag 
      tcgggcgac tccacgcct acgtccaac ggctcgcgc 
      aaaagcatc tcgcggacg gtctccaac cacgaaatc 
      caggccgac aagccgagc gcacttcta cccgtggag 
      aagctaacc aaaattttg gccgacaaa tcgtttgag 
      gaggagcac attaccggc atttccctc aatgttttc 
      accaagtgt ttgttcccg cggtacccc gtcttggcc 
      gagaagctc tacacgtac ttactcacg ggggcgaag 
      tcacgcaac acttacatc gacatccag ggcttcaaa 
      ctgcaatgc gagaaatac ctatcgatt ctggacgac 
      gaaacggta cgtgacact ttcgtacgc atgtttggc 
      acacaagtg gaagacgct ggcgaggtt ataaccccc 
      gaggggctc cgctcgctc ctaatgtgc gcctaccac 
      gtcagcatg gaccactac tccgagggc ccccagatg 
      tgcctctca attagcaag actttgaaa gctgtggtt 
      gatagttgc tttcacacc aaaacccaa ctctcaacc 
      cagtttgtt tcccattgg ctggccgcc aattgtccc 
      cgcctcctg ctcccccta cacagatat ctagtacat 
      tccttagcc acgtcgtgg cgcactttg gaagacatg 
      gagaacgcc ccagccgca ggtttagag ttggccacg 
      cccgtcctg gagcaggcc ccgcccttc agccagaaa 
      cacccccac ttgctgcac atgagcatg tcgtggctg 
      ttggcaggg gccctccca cctgtattt tcacgccca 
      caaaaagcg cactcgcct agcaacagt ggcgttggt 
      ctggcaagc accgccttt ttgaccaaa ctcttgtgc 
      tcggtgcct tctcattgg gtggttttg tacgactcg 
      gataatgac ggtttaggg gcgaatcgt ttcctacac 
      cacgtgatg tcgtacaaa gggcccact ttgtgtctt 
      ttgcgggtc gaagatggc caagttttt tgtattgcg 
      tcaccgaat gagtggaga gaatccaat cattattgg 
      ggtggggag gactcagct gtttttcaa ttgttacca 
      aagtttgtg ttactggaa aaggggtcc aaaatgttg 
      tatttaaat accacggtt cgggggtac ccttatggg 
      ttacgggct gggaaagac ccacgtagt cccataatc 
      atagtagac ggtgggttt gaaaagatg gagtttaaa 
      aaaataccc tacagtttg ctacgtatt gaagtctgg 
      ggttgtggg gatcccata agtcgggaa caacaatta 
      gaagtgaaa aaatgggaa gtcaaggaa gccgaaaga 
      caaagatgt gtcaaattg agcgccgat gattggctg 
      gaccatcca gataggtat ctgttggag ttggctggg 
      cggccacag taccaccaa aaccaaaca caataa
    mRNA sequence
    • >TC015511 RA
      gtcagcgat accaaccgc catagtaac ctaaaatga 
      gtcagtgat aaaaatcgg ggcgcaaga tggggaacc 
      accacggac acggccccc atagcaagt cgggcgact 
      ccacgccta cgtccaacg gctcgcgca aaagcatct 
      cgcggacgg tctccaacc acgaaatcc aggccgaca 
      agccgagcg cacttctac ccgtggaga agctaacca 
      aaattttgg ccgacaaat cgtttgagg aggagcaca 
      ttaccggca tttccctca atgttttca ccaagtgtt 
      tgttcccgc ggtaccccg tcttggccg agaagctct 
      acacgtact tactcacgg gggcgaagt cacgcaaca 
      cttacatcg acatccagg gcttcaaac tgcaatgcg 
      agaaatacc tatcgattc tggacgacg aaacggtac 
      gtgacactt tcgtacgca tgtttggca cacaagtgg 
      aagacgctg gcgaggtta taacccccg aggggctcc 
      gctcgctcc taatgtgcg cctaccacg tcagcatgg 
      accactact ccgagggcc cccagatgt gcctctcaa 
      ttagcaaga ctttgaaag ctgtggttg atagttgct 
      ttcacacca aaacccaac tctcaaccc agtttgttt 
      cccattggc tggccgcca attgtcccc gcctcctgc 
      tccccctac acagatatc tagtacatt ccttagcca 
      cgtcgtggc gcactttgg aagacatgg agaacgccc 
      cagccgcag gtttagagt tggccacgc ccgtcctgg 
      agcaggccc cgcccttca gccagaaac acccccact 
      tgctgcaca tgagcatgt cgtggctgt tggcagggg 
      ccctcccac ctgtatttt cacgcccac aaaaagcgc 
      actcgccta gcaacagtg gcgttggtc tggcaagca 
      ccgcctttt tgaccaaac tcttgtgct cggtgcctt 
      ctcattggg tggttttgt acgactcgg ataatgacg 
      gtttagggg cgaatcgtt tcctacacc acgtgatgt 
      cgtacaaag ggcccactt tgtgtcttt tgcgggtcg 
      aagatggcc aagtttttt gtattgcgt caccgaatg 
      agtggagag aatccaatc attattggg gtggggagg 
      actcagctg tttttcaat tgttaccaa agtttgtgt 
      tactggaaa aggggtcca aaatgttgt atttaaata 
      ccacggttc gggggtacc cttatgggt tacgggctg 
      ggaaagacc cacgtagtc ccataatca tagtagacg 
      gtgggtttg aaaagatgg agtttaaaa aaataccct 
      acagtttgc tacgtattg aagtctggg gttgtgggg 
      atcccataa gtcgggaac aacaattag aagtgaaaa 
      aatgggaag tcaaggaag ccgaaagac aaagatgtg 
      tcaaattga gcgccgatg attggctgg accatccag 
      ataggtatc tgttggagt tggctgggc ggccacagt 
      accaccaaa accaaacac aataagtat caattaagt 
      acttagaaa tttgttaga gtgatatta aagttaggc 
      tagggactg ttgtattta ttcaagtct cactcgcat 
      tcaaaagta ttgatacgt gccttgaga agacggctt 
      ttcgatatt attagctac ttaattaaa tttaatcaa 
      cttgcgtat ttcaagcta cttaattcg ttttattct 
      taacagggt gttgttcat tacagtgtt caagcagac 
      atattttta taaaaatgt ttgacccaa cgcgcgttt 
      tattcacgc aaattcatt cccactgtt tattccttt 
      aaaaaaaac atacaaata aaaatattt ttttaaaca 
      gacgtaacc ttaacctaa agtatattt ggttgcata 
      aacatccgg ttttgaggc gattttttg taattaatt 
      gtttaaaat ttacacaaa ttgatcaat aaaatgtat 
      ccaaccgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY