Status
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Gene information
General information
TC015509 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1637129..1640904
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015509
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10169

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015509 RA
      MAEENDSYP LHQAVFNND LKSLSRLLR KHDVGAKDK 
      HGNTALHLA VMLGRKECV QLLLKHDAP VKVKNGLGW 
      TVLAEAVSY GNRPTISSL VRKFRQQSR EQMEERRPN 
      LVEALNRIN DFYMELKWD FQSWVPLVS RILPSDICK 
      IYKSGANIR LDTTLVDFS DMRWERGDI SFIFNGNAE 
      PNESLTVLD NILKVYQKV RYEESEMEI EDEVDLLMS 
      TDILAAQVS TKSISFARA QSGWIFRED KKELVAGQY 
      ESELYTVNG LMLESRKRR EHLSSDDLQ KNKALLESF 
      TKGGSLQSF DQNGVPVRR TSLIPPPEK NVTWQEYIN 
      SEVNSYPRL GRDLVYKET TKAFKATIA MSSDFPLSV 
      EMLLNVLEV IAPFKHFSK LRDFITFKL PTGFPVKVE 
      IPILPTVTA KITFQQFEF RNNIPKELF ETPKNYKED 
      PTRFPDL
    CDS sequence
    • >TC015509 RA
      atggccgag gaaaatgac agttatcct ctccaccaa 
      gctgttttt aataacgat ttgaaatcc ctgtcccgc 
      ctcttgcgc aaacacgat gtgggggcc aaagacaag 
      cacggcaac acggccctg cacctggcc gtcatgttg 
      ggccgcaaa gaatgcgtg caattgctg ctgaaacac 
      gacgccccg gttaaggtg aaaaacggg ctgggatgg 
      acggtcctg gccgaggct gtgagctac ggcaacagg 
      ccgacgatc tcgtcgctg gtgcgcaag ttccggcag 
      cagtcgcgc gagcagatg gaggagcgg cgccccaat 
      ctggtcgag gcgctgaat aggattaac gatttttac 
      atggagctc aagtgggat ttccagtcg tgggtgccg 
      ctggtgtcg cggattttg ccctctgat atttgcaag 
      atttacaaa agtggggcc aatattcgg ctcgatacc 
      actctggtg gatttctcc gatatgcgg tgggaacgc 
      ggcgatatt tcgtttatt tttaacgga aatgccgaa 
      ccaaatgaa agtctaacc gtccttgat aacattttg 
      aaagtttat cagaaagtt cgctatgaa gaaagcgaa 
      atggagatt gaggatgag gttgatttg ttgatgtcg 
      acggatatt ttagctgct caagtgtcc acgaaaagt 
      atttcgttt gcgcgtgcg caaagtggc tggattttt 
      agagaggat aagaaggaa ttggttgcg ggacaatac 
      gagagcgag ttgtacaca gttaatggt ctcatgttg 
      gagagtcgg aaacgaaga gaacattta tcgtcggat 
      gatttacag aaaaataaa gcactattg gagagtttt 
      accaaaggc ggcagcttg cagagcttc gatcaaaat 
      ggcgttccg gtccgccgc acgagcctt atcccccct 
      cccgaaaaa aatgtcacg tggcaagaa tacataaat 
      tcggaggtg aattcttat cctcgcctt ggccgcgac 
      ctagtttac aaagagaca acaaaagcc ttcaaggcc 
      acgatagcc atgagttcg gactttccc ctctcggtg 
      gaaatgcta ctaaacgtc ctcgaggtc atcgccccc 
      ttcaaacac ttttccaag ctgcgtgac ttcatcaca 
      ttcaaactc ccgacgggt ttccccgtc aaagtcgag 
      attcccatc ttgccgaca gtgactgca aaaatcaca 
      ttccagcag ttcgagttt cggaacaac ataccgaaa 
      gagttgttc gaaactccc aaaaactat aaagaggat 
      ccgaccaga tttcccgat ttatga
    mRNA sequence
    • >TC015509 RA
      gaattgaaa aatggccga ggaaaatga cagttatcc 
      tctccacca agctgtttt taataacga tttgaaatc 
      cctgtcccg cctcttgcg caaacacga tgtgggggc 
      caaagacaa gcacggcaa cacggccct gcacctggc 
      cgtcatgtt gggccgcaa agaatgcgt gcaattgct 
      gctgaaaca cgacgcccc ggttaaggt gaaaaacgg 
      gctgggatg gacggtcct ggccgaggc tgtgagcta 
      cggcaacag gccgacgat ctcgtcgct ggtgcgcaa 
      gttccggca gcagtcgcg cgagcagat ggaggagcg 
      gcgccccaa tctggtcga ggcgctgaa taggattaa 
      cgattttta catggagct caagtggga tttccagtc 
      gtgggtgcc gctggtgtc gcggatttt gccctctga 
      tatttgcaa gatttacaa aagtggggc caatattcg 
      gctcgatac cactctggt ggatttctc cgatatgcg 
      gtgggaacg cggcgatat ttcgtttat ttttaacgg 
      aaatgccga accaaatga aagtctaac cgtccttga 
      taacatttt gaaagttta tcagaaagt tcgctatga 
      agaaagcga aatggagat tgaggatga ggttgattt 
      gttgatgtc gacggatat tttagctgc tcaagtgtc 
      cacgaaaag tatttcgtt tgcgcgtgc gcaaagtgg 
      ctggatttt tagagagga taagaagga attggttgc 
      gggacaata cgagagcga gttgtacac agttaatgg 
      tctcatgtt ggagagtcg gaaacgaag agaacattt 
      atcgtcgga tgatttaca gaaaaataa agcactatt 
      ggagagttt taccaaagg cggcagctt gcagagctt 
      cgatcaaaa tggcgttcc ggtccgccg cacgagcct 
      tatcccccc tcccgaaaa aaatgtcac gtggcaaga 
      atacataaa ttcggaggt gaattctta tcctcgcct 
      tggccgcga cctagttta caaagagac aacaaaagc 
      cttcaaggc cacgatagc catgagttc ggactttcc 
      cctctcggt ggaaatgct actaaacgt cctcgaggt 
      catcgcccc cttcaaaca cttttccaa gctgcgtga 
      cttcatcac attcaaact cccgacggg tttccccgt 
      caaagtcga gattcccat cttgccgac agtgactgc 
      aaaaatcac attccagca gttcgagtt tcggaacaa 
      cataccgaa agagttgtt cgaaactcc caaaaacta 
      taaagagga tccgaccag atttcccga tttatgacg 
      tcctgaatt gttgaattc gttcgaaat aacttatac 
      taaaaaacc agtgttcct cctgccgta cgacagacg 
      tctcctggc caaggcgaa gtttgtcgg taaattaag 
      aaaatcaaa ggagtcggg aggggggca gagccccac 
      cgtgtgcgt ggaagtccc gatgctgtt cacgctcgg 
      cagcatcta ctcgactag ctgtctagt ctatctgga 
      ataaatttc gattcgttt tctctccaa aagcagggt 
      atttgtttt attctaggt tttaacacg gaaatttgt 
      tgtgttttt cgttgaatc gttgttact tgtgtattc 
      atgttggat gtttagtgt ttgccggca ccgacatat 
      caggaaagg aagatttct acttattta ttcgttagg 
      tcgcaaatg tcaattaca gaacaagac taatttacc 
      acaggctga cgtattatt tatgcttac taatcatcg 
      ccattactt gtatttatt acattaaca gtaggtaat 
      tgttccatt ttcgcaata aaatgttta attttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY