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Gene information
General information
TC015210 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 1466477..1467676
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015210
    iBeetle screen iB_10166

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015210 RA
      MCKFLLIVV TTSLTLKLI LAFCEESYV IICDDLIDI 
      EELQTDGAV SWRHVLIGS NHYKSEPPN HILTYKHIT 
      ALSRTKSLT ILNQIKLIT SNAFGFSNN LLQHLNFFN 
      NKIATIRDH SFAKLENLR EVYLENNEI HHLGRAIFK 
      GTSLEIVNL SRNKLESIT TAFLETRIK WLLLNHNNL 
      SFIEARAFP DNLEQLELN HNNLEDLES NLFQNLHNL 
      KELFIEHNL LKTVPDIAP LTRIQYLHF AHNQIQKVY 
      YRFDKHETL EFVDFSSNF IRNVATEVF SSDSPPLVI 
      LTFNRLTNL EFNNWEKKT DLGLYGNPW NCDCLFKIE 
      TNLRKYKHL SSCEFPRSL NGKLPICVE GGKCEDVDE 
      IKEADISRF REETTYFED KIDCYFRL
    CDS sequence
    • >TC015210 RA
      atgtgtaag tttttattg atcgttgta actacttct 
      ttaacattg aaactaata ctggctttc tgtgaagag 
      tcgtatgtt ataatatgt gacgattta atcgacatt 
      gaagaacta caaactgac ggtgctgta agttggaga 
      catgtcctg attggctca aatcattac aaatccgaa 
      cctccaaac cacattctg acttacaaa cacattact 
      gccttatct cgaacaaaa tctttgaca attctcaat 
      caaatcaag ttgataact tcaaatgca ttcggattt 
      agcaacaac ttgctacaa cacttgaac ttcttcaac 
      aacaaaatt gcaacaatt cgggaccac agtttcgca 
      aaattagaa aatctccgc gaagtctat ttagaaaac 
      aacgaaatt caccatttg ggacgcgca attttcaaa 
      ggtacaagt ctggagatt gtgaatttg tcacgcaac 
      aaactcgaa tccatcact accgctttt ttggaaact 
      aggataaag tggcttttg ttaaaccac aataacttg 
      agttttatt gaagctaga gccttccca gacaatttg 
      gaacaactc gagttgaat cacaacaat ctggaggat 
      ttagaaagc aatcttttc caaaacctc cacaacttg 
      aaagaactc ttcatcgaa cacaactta ctgaaaacg 
      gtgccggac atcgccccc ttaacacgc atccaatac 
      ctacatttt gcccacaac caaattcag aaagtttac 
      tatcggttt gacaaacac gaaacttta gaattcgtg 
      gattttagc tcgaatttc atacgaaat gtggccacc 
      gaagtgttc agttctgat tctcctccg ttggtgatt 
      ttgactttt aatagactg accaatttg gagtttaac 
      aactgggag aaaaaaact gatttgggg ctttatggt 
      aatccgtgg aattgtgac tgtttgttt aaaattgaa 
      accaacttg aggaagtac aaacatttg agttcgtgc 
      gaattcccg cggtcgctt aacgggaaa ttaccaatt 
      tgcgtcgaa ggcggcaaa tgtgaggat gtggacgag 
      atcaaggag gcggatatt tcgcgattt cgggaagaa 
      accacgtat tttgaggac aaaatcgac tgttatttt 
      cgactatga
    mRNA sequence
    • >TC015210 RA
      taagtaggt actagtcac catgtgtaa gtttttatt 
      gatcgttgt aactacttc tttaacatt gaaactaat 
      actggcttt ctgtgaaga gtcgtatgt tataatatg 
      tgacgattt aatcgacat tgaagaact acaaactga 
      cggtgctgt aagttggag acatgtcct gattggctc 
      aaatcatta caaatccga acctccaaa ccacattct 
      gacttacaa acacattac tgccttatc tcgaacaaa 
      atctttgac aattctcaa tcaaatcaa gttgataac 
      ttcaaatgc attcggatt tagcaacaa cttgctaca 
      acacttgaa cttcttcaa caacaaaat tgcaacaat 
      tcgggacca cagtttcgc aaaattaga aaatctccg 
      cgaagtcta tttagaaaa caacgaaat tcaccattt 
      gggacgcgc aattttcaa aggtacaag tctggagat 
      tgtgaattt gtcacgcaa caaactcga atccatcac 
      taccgcttt tttggaaac taggataaa gtggctttt 
      gttaaacca caataactt gagttttat tgaagctag 
      agccttccc agacaattt ggaacaact cgagttgaa 
      tcacaacaa tctggagga tttagaaag caatctttt 
      ccaaaacct ccacaactt gaaagaact cttcatcga 
      acacaactt actgaaaac ggtgccgga catcgcccc 
      cttaacacg catccaata cctacattt tgcccacaa 
      ccaaattca gaaagttta ctatcggtt tgacaaaca 
      cgaaacttt agaattcgt ggattttag ctcgaattt 
      catacgaaa tgtggccac cgaagtgtt cagttctga 
      ttctcctcc gttggtgat tttgacttt taatagact 
      gaccaattt ggagtttaa caactggga gaaaaaaac 
      tgatttggg gctttatgg taatccgtg gaattgtga 
      ctgtttgtt taaaattga aaccaactt gaggaagta 
      caaacattt gagttcgtg cgaattccc gcggtcgct 
      taacgggaa attaccaat ttgcgtcga aggcggcaa 
      atgtgagga tgtggacga gatcaagga ggcggatat 
      ttcgcgatt tcgggaaga aaccacgta ttttgagga 
      caaaatcga ctgttattt tcgactatg aaataaacg 
      agttctagc gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY