Status
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Gene information
General information
TC015236 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9378575..9384623
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015236
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10165

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015236 RA
      MTKYSMNDT NSTTGSQVT LTSYMIGEN LDEKDTKGI 
      TVLDLPQER KTPSPGFAK WVKNRARRG CTRKLVYKR 
      VPILTWLPK YNVSTAVAD LVAGFTVGL TVIPQGIAY 
      SNVAGLPPQ IGLYSSFMA CFVYTIFGS CRESPIGPT 
      AIAGLLTRE NTHGMGVSG AVLLCFLSG CVEFLMGLL 
      QLGFLIDFI SGPVSIGFT SAAAIIIAT TQVKDVLGL 
      DYPGGKFLQ VWEQIFQHI TETRLWDCI LGLTCMAVL 
      LILRSIKDL KIGPQDVKE RRPIHDFAT KFIWLISTA 
      RNIFVVVLS ALLAYFFEV HGSQPFILT GFIKPGLPE 
      FKPPPFEMR IDNTTYNFV DMSSALGSA LLVVPLLSI 
      LENIALAKV FADGKTIDA TQEMLALGI CNIASSFVQ 
      SMPVSGALS RGAVNHASG VKTTFGGVY TGIIVILSL 
      HLFTPYFSY IPKASLAAV IIAAVVFMV EFHVIKPIW 
      RTKKSDLIP ACTTFVCCL FLRLEIGIV VGVGINLIF 
      LLYATARPS VHVEKVSAY SGCDYLLIT PDRSLTFPS 
      VEYVRTVVS KAGVKQGSS SIPVVIDAR HIQGADFTA 
      ARGIKSLIE DFHKRKQPI LFYNLKPSV ISTFQGVQP 
      KDFIYCETY FELNELLKR YSGKSESIS IQNSDN
    CDS sequence
    • >TC015236 RA
      atgaccaaa tacagcatg aacgacacg aactccacc 
      accgggagc caagtcacc ctgacctcc tacatgatt 
      ggggaaaat ctcgacgag aaagacacc aaggggatc 
      accgtgctt gacctgccg caagagcgg aaaaccccc 
      tcgcctggg tttgccaag tgggtgaag aaccgggcc 
      aggaggggc tgcaccagg aagctggtc tacaagagg 
      gtgcccatt ttgacgtgg ttgcccaaa tacaacgtc 
      agcacagcc gtcgctgac ctggtggcc ggcttcacc 
      gtcgggctc acggtgatt ccccaggga atcgcctat 
      tcgaacgtg gccggcctg ccgccacaa ataggcctt 
      tattcctcc ttcatggcc tgcttcgtc tacaccatc 
      ttcggcagc tgccgggag tcgcccatt gggcctacg 
      gccatcgct gggctcctc acccgggaa aacacccat 
      ggcatgggt gtcagcggc gccgtcctc ttgtgcttc 
      ctcagcggc tgtgtggag ttcctcatg ggcctcctc 
      cagctgggt tttttgatc gatttcatc tcaggcccg 
      gtgtccatc gggttcacg tcagcggcc gccatcatc 
      atcgccacg acccaagtc aaagacgtc ctaggcctg 
      gattatcca ggagggaag ttcctgcaa gtttgggag 
      caaattttc cagcacatt actgagacc aggctctgg 
      gactgcatt ctgggcctg acttgcatg gccgtgttg 
      ctgattttg aggagcatc aaggacctc aaaataggg 
      ccgcaggac gtgaaggag cgccggccc attcacgac 
      ttcgccacc aagttcatc tggctgata tcaaccgcg 
      cgcaacatt ttcgtagtt gtgctgtcg gcccttctg 
      gcctacttc ttcgaagtg cacggttcg cagcccttc 
      atcctcacc ggattcatc aaacccgga ctcccggag 
      ttcaagccg cccccgttc gagatgcgg atcgacaac 
      acaacgtac aacttcgtc gacatgtcc tcggccctc 
      ggttcggcc cttttggtc gtgcccttg ttgtcgatt 
      ttggaaaac atcgccttg gccaaagtt ttcgctgac 
      gggaaaacc atcgacgca acccaggaa atgttggct 
      ttggggatc tgcaacatt gcgagttct ttcgtccag 
      tcgatgcct gtctcgggg gcgttgtcc cggggggct 
      gtcaaccac gccagtggg gttaaaacg acctttggc 
      ggggtctac actggaatc attgtaatt ctatcctta 
      catctcttc actccttat ttctcttat attcccaaa 
      gcttcccta gctgctgtc attatcgcc gcggtggtg 
      tttatggtc gagtttcac gtcattaag ccaatttgg 
      cgcactaag aagtccgat ttaataccc gcctgtaca 
      acattcgtt tgttgcctg tttttgcgt ctagaaatc 
      gggattgta gtgggagtt gggattaat ctaatcttc 
      ctgctgtat gcaacagca cggccgtca gttcacgtg 
      gaaaaagtt tcggcgtat tctgggtgc gactatctc 
      ctaatcacc cccgaccga agcctgact tttccctca 
      gtcgagtat gtcaggact gtggtgtct aaagccgga 
      gtcaaacaa ggctcaagt tcgattccc gtggtcata 
      gacgcgagg catatacag ggtgctgat tttacagca 
      gcgcgaggg atcaagtcg ctgattgaa gattttcac 
      aaaaggaag caaccgatt ttgttctac aatttgaaa 
      ccgagtgtc ataagtact tttcaaggt gttcaaccc 
      aaagacttt atttattgt gaaacttac ttcgaattg 
      aacgaactc ttgaagcgc tattcgggc aagtccgaa 
      tcgatttcg atacaaaat agtgataat taa
    mRNA sequence
    • >TC015236 RA
      aaaccgaac aattctcat gtgctttcc agtcggttt 
      agttcagtc tagtggcaa agttggtcg cgagtagct 
      cgtgaaagc agttcgggc acatctgac ttaagttaa 
      gtggactta gggtggaaa ggctttcgg cgcaaaaca 
      tgtcggaaa taaatagca attaagccg aatgtcaac 
      aacatccca tgaccttgc accatgacc aaatacagc 
      atgaacgac acgaactcc accaccggg agccaagtc 
      accctgacc tcctacatg attggggaa aatctcgac 
      gagaaagac accaagggg atcaccgtg cttgacctg 
      ccgcaagag cggaaaacc ccctcgcct gggtttgcc 
      aagtgggtg aagaaccgg gccaggagg ggctgcacc 
      aggaagctg gtctacaag agggtgccc attttgacg 
      tggttgccc aaatacaac gtcagcaca gccgtcgct 
      gacctggtg gccggcttc accgtcggg ctcacggtg 
      attccccag ggaatcgcc tattcgaac gtggccggc 
      ctgccgcca caaataggc ctttattcc tccttcatg 
      gcctgcttc gtctacacc atcttcggc agctgccgg 
      gagtcgccc attgggcct acggccatc gctgggctc 
      ctcacccgg gaaaacacc catggcatg ggtgtcagc 
      ggcgccgtc ctcttgtgc ttcctcagc ggctgtgtg 
      gagttcctc atgggcctc ctccagctg ggttttttg 
      atcgatttc atctcaggc ccggtgtcc atcgggttc 
      acgtcagcg gccgccatc atcatcgcc acgacccaa 
      gtcaaagac gtcctaggc ctggattat ccaggaggg 
      aagttcctg caagtttgg gagcaaatt ttccagcac 
      attactgag accaggctc tgggactgc attctgggc 
      ctgacttgc atggccgtg ttgctgatt ttgaggagc 
      atcaaggac ctcaaaata gggccgcag gacgtgaag 
      gagcgccgg cccattcac gacttcgcc accaagttc 
      atctggctg atatcaacc gcgcgcaac attttcgta 
      gttgtgctg tcggccctt ctggcctac ttcttcgaa 
      gtgcacggt tcgcagccc ttcatcctc accggattc 
      atcaaaccc ggactcccg gagttcaag ccgcccccg 
      ttcgagatg cggatcgac aacacaacg tacaacttc 
      gtcgacatg tcctcggcc ctcggttcg gcccttttg 
      gtcgtgccc ttgttgtcg attttggaa aacatcgcc 
      ttggccaaa gttttcgct gacgggaaa accatcgac 
      gcaacccag gaaatgttg gctttgggg atctgcaac 
      attgcgagt tctttcgtc cagtcgatg cctgtctcg 
      ggggcgttg tcccggggg gctgtcaac cacgccagt 
      ggggttaaa acgaccttt ggcggggtc tacactgga 
      atcattgta attctatcc ttacatctc ttcactcct 
      tatttctct tatattccc aaagcttcc ctagctgct 
      gtcattatc gccgcggtg gtgtttatg gtcgagttt 
      cacgtcatt aagccaatt tggcgcact aagaagtcc 
      gatttaata cccgcctgt acaacattc gtttgttgc 
      ctgtttttg cgtctagaa atcgggatt gtagtggga 
      gttgggatt aatctaatc ttcctgctg tatgcaaca 
      gcacggccg tcagttcac gtggaaaaa gtttcggcg 
      tattctggg tgcgactat ctcctaatc acccccgac 
      cgaagcctg acttttccc tcagtcgag tatgtcagg 
      actgtggtg tctaaagcc ggagtcaaa caaggctca 
      agttcgatt cccgtggtc atagacgcg aggcatata 
      cagggtgct gattttaca gcagcgcga gggatcaag 
      tcgctgatt gaagatttt cacaaaagg aagcaaccg 
      attttgttc tacaatttg aaaccgagt gtcataagt 
      acttttcaa ggtgttcaa cccaaagac tttatttat 
      tgtgaaact tacttcgaa ttgaacgaa ctcttgaag 
      cgctattcg ggcaagtcc gaatcgatt tcgatacaa 
      aatagtgat aattaatag tgattgtga taagtaggg 
      atcaatgat ttagacagt gacaaatga cgaatttta 
      tagagtatt aagtacctc acaagtcta ctgtagttt 
      agattactt tagtgttac gaaatgtga tcttgattt 
      tagttaaga aattatttt ttaaatgat aattttatt 
      gtcctgttg taatattat tttgtataa gccgttcaa 
      taaattttt taaattgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY