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Gene information
General information
TC013591 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3329990..3331147
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013591
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10160

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    previtellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    Comment: In two case (ovary), randomly either vitellogenic or previtellogenic late egg chamber of different ovarioles are not present .
    lateral oviduct degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013591 RA
      MLIVLFIPL FIVALALRL IKQRTPKSL DKRHVVITG 
      GSSGIGKST AIQAARKGA NVTIIARNL DRLSAAEKE 
      IRGACIHKD QIITAVSVD VSNKTSIQK ELTNVEEKV 
      GPIYMLVNC AGMAICGKV EDFTEQDVK TLINANYLG 
      TLYPIQSVV PRFKDRQEG IIVLTASQV ALMGMYGYS 
      IYASCKFAL RGLAESLSM ELKPYNVSV TLALPPDTD 
      TPGYENENK TKPTETQLI SEAGGLLKP EVVAEKLLK 
      DALTQKFFS YVGLESYIL TTLCVGMTP CADLLELMI 
      QSVLLGPLR LIGAYYLRT FHSIIQRCH LQKQKEN
    CDS sequence
    • >TC013591 RA
      atgttgata gttttattc atccccctg ttcatagtg 
      gcgttagca ttacgcctg ataaagcaa cgaaccccc 
      aaaagctta gataaacga cacgttgtt attacagga 
      ggttccagt ggaattggc aaaagtaca gctattcaa 
      gccgccaga aaaggcgca aacgttact ataattgcc 
      cgaaatttg gacagacta tcagcggct gaaaaagaa 
      atcagggga gcttgcatt cacaaagac caaataatc 
      acagctgta tcggtggat gtttccaat aaaaccagc 
      atccagaag gaactgacc aacgttgag gaaaaagtg 
      ggcccaatt tacatgctg gtcaactgt gccggaatg 
      gcgatttgt ggcaaagtt gaggatttc acagaacaa 
      gacgttaaa acactgatc aatgccaat tatttgggt 
      actctgtac cctattcaa agtgtagta ccgagattc 
      aaagaccgg caagaggga attatcgtg ctaacagcc 
      tcacaagtg gccctaatg ggcatgtac gggtactca 
      atttacgcc agttgtaaa tttgcgttg cgaggactt 
      gccgagtca ctcagcatg gaacttaag ccctacaac 
      gtctccgta accctagcc ctacctccc gacaccgac 
      actccggga tatgagaat gaaaacaag actaaaccg 
      actgaaaca caactaata tctgaggcc ggcggtctg 
      cttaaacca gaagttgta gctgagaaa ctcctcaaa 
      gatgccttg acacagaaa tttttcagt tacgtgggg 
      cttgagagt tatattttg accacgtta tgtgtcgga 
      atgacaccc tgtgctgac ttactcgaa cttatgatt 
      cagtcggtt cttttaggg ccattgcga ctcattggc 
      gcttactat ttacgcaca tttcatagt attattcaa 
      aggtgtcac ctacagaag caaaaagaa aactga
    mRNA sequence
    • >TC013591 RA
      tgaggttta tgggtgttg aatcatggg aataacgtg 
      gtgttagtg tcacaccta cctacagta acccagtaa 
      agtgcccca aagttgtga tcgaaaatg ttgatagtt 
      ttattcatc cccctgttc atagtggcg ttagcatta 
      cgcctgata aagcaacga acccccaaa agcttagat 
      aaacgacac gttgttatt acaggaggt tccagtgga 
      attggcaaa agtacagct attcaagcc gccagaaaa 
      ggcgcaaac gttactata attgcccga aatttggac 
      agactatca gcggctgaa aaagaaatc aggggagct 
      tgcattcac aaagaccaa ataatcaca gctgtatcg 
      gtggatgtt tccaataaa accagcatc cagaaggaa 
      ctgaccaac gttgaggaa aaagtgggc ccaatttac 
      atgctggtc aactgtgcc ggaatggcg atttgtggc 
      aaagttgag gatttcaca gaacaagac gttaaaaca 
      ctgatcaat gccaattat ttgggtact ctgtaccct 
      attcaaagt gtagtaccg agattcaaa gaccggcaa 
      gagggaatt atcgtgcta acagcctca caagtggcc 
      ctaatgggc atgtacggg tactcaatt tacgccagt 
      tgtaaattt gcgttgcga ggacttgcc gagtcactc 
      agcatggaa cttaagccc tacaacgtc tccgtaacc 
      ctagcccta cctcccgac accgacact ccgggatat 
      gagaatgaa aacaagact aaaccgact gaaacacaa 
      ctaatatct gaggccggc ggtctgctt aaaccagaa 
      gttgtagct gagaaactc ctcaaagat gccttgaca 
      cagaaattt ttcagttac gtggggctt gagagttat 
      attttgacc acgttatgt gtcggaatg acaccctgt 
      gctgactta ctcgaactt atgattcag tcggttctt 
      ttagggcca ttgcgactc attggcgct tactattta 
      cgcacattt catagtatt attcaaagg tgtcaccta 
      cagaagcaa aaagaaaac tgaatatct gttgtaaat 
      tagcaaaat aaagtattt gttctgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY