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Gene information
General information
TC015429 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 777331..780146
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015429
    iBeetle screen iB_10159

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015429 RA
      MSHYSDAED SIDIAADMG VRKRRIWDP ETESITSEI 
      KELLDDDVN SLVEEDCVG CPLPSTPED ENMLDSEMS 
      DVLKAAVLG TGDELDITT LAHNAASQA EHVVRKMIQ 
      AGWSVCHFR NLPAWLQDN DFLIFGHRP PLPSFRACF 
      KSIFRIHTE TANIWTHLL GCVAFIAIA IFFLMRPSM 
      EIELQEKIV FGAFFAGAI ICLGMSFMF HTVNCHSQF 
      IGKLFSKLD YCGIAMLIM GSFVPWVYY GFYCHFRPK 
      VVYLSVVCA LGITSIMVS LWDKFSESG WRPFRAAVF 
      MTFGLSGIV PAIHYGIVE GWFNKVSQK SLGWLILMG 
      LLYIMGAML YALRVPERW FPGKFDIWL HSHQIFHVF 
      VLGGAFVHY HGISEMAMY RVTIGQCEM PDIPIY
    CDS sequence
    • >TC015429 RA
      atgtcgcac tatagtgat gccgaagac tccattgac 
      atagccgcc gatatgggg gtgcgtaag cggaggatc 
      tgggaccct gagaccgaa tcgatcact tcggaaatc 
      aaggaactg ctagacgat gatgttaat tcgctggtt 
      gaggaggat tgtgtgggg tgccccctg ccctccacc 
      cccgaagac gaaaatatg ctcgattcg gagatgagc 
      gatgtcttg aaagccgcc gttttaggt acaggtgac 
      gagctcgac atcaccacg ttggcccac aatgccgcc 
      agccaggcg gagcacgtg gtgcgtaaa atgatacaa 
      gctggttgg tccgtttgt cacttccgt aaccttccc 
      gcttggctt caagacaac gatttcttg atttttggc 
      catcgtccg ccgcttcct agttttcgc gcttgtttt 
      aaaagcatt tttcgcatc cacaccgaa accgctaat 
      atttggaca catcttctc ggatgcgtt gcgtttata 
      gctattgct atctttttc ctgatgcgt ccctcgatg 
      gaaattgag cttcaggag aagattgtt tttggggca 
      ttttttgcc ggggctata atttgcctg gggatgtct 
      ttcatgttc catacagtc aactgtcat tcgcaattt 
      atcggtaaa ctattttcg aaattggat tattgcggg 
      attgccatg ttgataatg ggctcgttt gtgccatgg 
      gtttattat gggttctac tgccatttc cgaccaaag 
      gttgtctac ctatcggtg gtgtgtgcg ctagggatt 
      accagcatc atggttagt ttatgggac aagttctcg 
      gagtctggt tggaggccg ttcagagct gctgttttc 
      atgacgttt ggactcagt gggatcgta ccagcgatc 
      cattatggg attgtggaa ggatggttc aataaagtg 
      agtcagaag tcgctcgga tggctgatt ctgatgggg 
      ctgttgtac atcatgggg gccatgttg tatgcactg 
      cgagtgcca gagcggtgg ttccccggg aagttcgat 
      atctggctg cactcgcac caaattttc catgttttt 
      gtgctaggg ggcgccttc gtgcattac catggaata 
      tcagagatg gccatgtat cgggtgacc atcggccag 
      tgcgaaatg cccgatatt cccatctat taa
    mRNA sequence
    • >TC015429 RA
      agttttgta accaagcct ccacacaca gctgatttt 
      gccacttgt caaaccatt taacaaatt tccagtgtc 
      tgtttactg aaaaccgtg tgttcaagg aggtaagcc 
      ccccagaaa ccggctgtg atttgggga caatgtcgc 
      actatagtg atgccgaag actccattg acatagccg 
      ccgatatgg gggtgcgta agcggagga tctgggacc 
      ctgagaccg aatcgatca cttcggaaa tcaaggaac 
      tgctagacg atgatgtta attcgctgg ttgaggagg 
      attgtgtgg ggtgccccc tgccctcca cccccgaag 
      acgaaaata tgctcgatt cggagatga gcgatgtct 
      tgaaagccg ccgttttag gtacaggtg acgagctcg 
      acatcacca cgttggccc acaatgccg ccagccagg 
      cggagcacg tggtgcgta aaatgatac aagctggtt 
      ggtccgttt gtcacttcc gtaaccttc ccgcttggc 
      ttcaagaca acgatttct tgatttttg gccatcgtc 
      cgccgcttc ctagttttc gcgcttgtt ttaaaagca 
      tttttcgca tccacaccg aaaccgcta atatttgga 
      cacatcttc tcggatgcg ttgcgttta tagctattg 
      ctatctttt tcctgatgc gtccctcga tggaaattg 
      agcttcagg agaagattg tttttgggg cattttttg 
      ccggggcta taatttgcc tggggatgt ctttcatgt 
      tccatacag tcaactgtc attcgcaat ttatcggta 
      aactatttt cgaaattgg attattgcg ggattgcca 
      tgttgataa tgggctcgt ttgtgccat gggtttatt 
      atgggttct actgccatt tccgaccaa aggttgtct 
      acctatcgg tggtgtgtg cgctaggga ttaccagca 
      tcatggtta gtttatggg acaagttct cggagtctg 
      gttggaggc cgttcagag ctgctgttt tcatgacgt 
      ttggactca gtgggatcg taccagcga tccattatg 
      ggattgtgg aaggatggt tcaataaag tgagtcaga 
      agtcgctcg gatggctga ttctgatgg ggctgttgt 
      acatcatgg gggccatgt tgtatgcac tgcgagtgc 
      cagagcggt ggttccccg ggaagttcg atatctggc 
      tgcactcgc accaaattt tccatgttt ttgtgctag 
      ggggcgcct tcgtgcatt accatggaa tatcagaga 
      tggccatgt atcgggtga ccatcggcc agtgcgaaa 
      tgcccgata ttcccatct attaaccaa agatcttga 
      tttttttaa ataaaattt tgcaaatcg aatgatttg 
      tgctttatg ttatgtcat tggttgttt attgttacg 
      tgtaagata tatagcaca agtcaagga cgagtgcgt 
      ttttagttc acacctagc ggtaaccaa gctttggat 
      aaggtttag tataaaaat gagaaaaaa atatatttt 
      gctattatc accgacatt tttgagaaa ggtatatcc 
      tcttgtaag tgtactatt atatatcaa tcttgtata 
      aaaaatgat acggagttt aggtatatt tttaggtgt 
      cttatctgt acttatctg ggtcttgca tttttttca 
      cagtaaaat tacgctgta gtttagtta caattttgt 
      gataatttc agtagaacc tctctattt attgttaca 
      cctcaattt tgttgtagg tattgtttt taaataaat 
      tgtaatgtc agatggaat atatcatgt gaccaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY