Status
Please wait ...
Gene information
General information
TC015304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 737608..740174
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015304
    iBeetle screen iB_10157

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015304 RA
      MNRTFLALL ALTSGAACD VAHLVHVQQ 
      TEPSTPPPP PKPYAFGYA AGRFPGHID 
      RTHSEVSDG SGVVQGSYS YVDPRFKIR 
      TVEYTADKN GFHPVLNFA PEPLPSDTP 
      VVAAAKHRH LVQFANIAN ARHAAPGTV 
      VVPVDSVAV SRAKDKHFK LFQKIAEEH 
      ARLAAAQEA QGGTEKPIY
    CDS sequence
    • >TC015304 RA
      atgaataga acgtttttg gccctgttg gcgctgacc 
      tcgggggcg gcgtgcgac gtcgcccac ttggtgcac 
      gtccagcaa accgagccg tcaacaccg ccgccgccc 
      ccaaagccg tacgctttt ggttacgcc gccggtcgt 
      ttccccggc cacatcgac cggacgcat tcggaggtt 
      tccgacggt tcgggggtg gtccaaggc tcgtactcg 
      tatgtggat ccccggttt aagatccgc actgttgag 
      tacacggcc gataagaac ggctttcac ccggttttg 
      aatttcgcg ccagagccg ctcccatcc gacacgccg 
      gtggtggcg gcggccaag caccgccat ctcgtgcag 
      ttcgcaaac atcgccaac gccaggcat gccgccccc 
      gggacggtc gtggtgcca gtcgactcg gtggcggtg 
      agccgcgcc aaggacaag catttcaag ctgttccag 
      aaaatcgca gaggagcat gccaggctg gcggcggcg 
      caggaggcg caagggggc accgaaaag cccatttat 
      tga
    mRNA sequence
    • >TC015304 RA
      atagctgaa ataattaaa cacaatgtc ggaggcttt 
      gaataagaa aagcaaaca atgaaacga ttgctatta 
      ttccacgta attacagct gaatacgtc gatatttca 
      gcgtatagg caaccagtt gggcgaata gattcgtat 
      cccaagtag gcaacgttt tggaaaatt tattccggc 
      cataatatc acacgtcgt cgccgttaa agtcggcga 
      aatttgcaa gtcgcgata aagatcatc gatttaaag 
      cataggtta atccgttcc atttcgtta tcggtcacg 
      ttccgaaac aaatggacg aatacgttt cgaaaatca 
      taaagcgcc atcgttgtg tcgtggctc taagtttga 
      taaatgcga ggatctgga cggagaatt aatggatgt 
      gtcacggct tcgccttaa aaagggtgc aatttgttt 
      ttttcctca tgtctagag gtggttttc ccaaagaaa 
      acgaagaaa aattgaaat gaaattctg tgattgcgt 
      aaccacgcc cacttcggg ccgggaaat ttaaaaaat 
      ttgattttc tgccacttt cattaataa ttgacgacc 
      tcgggggtt tacccaaat ctgcggaat ttatgtacg 
      agttgtacg tctttgacc cgaaaagcg atccgatta 
      cgggaatgc ctgattatt gctattatt acccgacta 
      aatgaattt atggccgga ggataacct tttttgttc 
      caagggaag cgaagaaaa gatccggag ggtgattta 
      ttggcccta tagtattgt tggttgcat aaccaataa 
      cgaacgtag cgagtgcgc caattgtat tactttcag 
      taaaatgtt tctggtaat attgacaat tgcaaacat 
      tatttgaat aacccgatt taatccaca atgtactac 
      cgattgctg tttttattg tgtattgtt ttttgttgt 
      aattaattc caatcggca aagctcgtc caaatgtta 
      aataaacaa tgtgtttgt tgattattt tatctgata 
      ttaacgaat tagcgctcg aacggaact ctgcagcag 
      acattaatt atttgcagt ttataaatt ataataatg 
      agggaggat aacatgtaa ttatttact agtttcaaa 
      tttattttg ggtaaatag aaatatatt gatgggcgt 
      ggccaattc aagaccagt cgtaccaac gcaaattaa 
      gtcaggcat gagatcatt ccagtatca tcttcacgg 
      ccttgactt ttctccgtt taagaataa attcatcgc 
      aaaatgaaa aagaaatca caaacacgt ttgtgtgtc 
      cgtgtgttg tttctcagt cacgagatt gtgtgagtg 
      acgcaattt accaacttt cactcaaaa cgcaaatct 
      gattttagt cattttgcc acaacgaca agaagtgca 
      tcggtgtgt ttgacaaaa aaaacgcaa tacaatgaa 
      tagaacgtt tttggccct gttggcgct gacctcggg 
      ggcggcgtg cgacgtcgc ccacttggt gcacgtcca 
      gcaaaccga gccgtcaac accgccgcc gcccccaaa 
      gccgtacgc ttttggtta cgccgccgg tcgtttccc 
      cggccacat cgaccggac gcattcgga ggtttccga 
      cggttcggg ggtggtcca aggctcgta ctcgtatgt 
      ggatccccg gtttaagat ccgcactgt tgagtacac 
      ggccgataa gaacggctt tcacccggt tttgaattt 
      cgcgccaga gccgctccc atccgacac gccggtggt 
      ggcggcggc caagcaccg ccatctcgt gcagttcgc 
      aaacatcgc caacgccag gcatgccgc ccccgggac 
      ggtcgtggt gccagtcga ctcggtggc ggtgagccg 
      cgccaagga caagcattt caagctgtt ccagaaaat 
      cgcagagga gcatgccag gctggcggc ggcgcagga 
      ggcgcaagg gggcaccga aaagcccat ttattgaat 
      tgtttaatt gcgaataaa gttttttga gcaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY