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Gene information
General information
TC033306 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 206644..207679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033306
    iBeetle screen iB_10149

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033306 RA
      MVALTACLF FLFATEAMS MLNGAPKPT YKNDILAIK 
      HIKDSVVLK SCIGVEIST FWVLSSALC LHSNISDKV 
      VVEARVNEE KVLRYTNTE VKIHPDYDK MSGNNDIGV 
      LHIKGGKHI EGMGQHLIL PFAHFPKMN GSISAWNLK 
      SGGYSQIVE SLEAGHLKV NIYLMTDCK LQFPQLDAQ 
      AHLHGDYQI CTFPMVEGS DLCEPDSGA PLYSYHKFS 
      NRWGLIGIY NDIMAATTG RTHQCNQEH RHPLIFTQI 
      FHYFGWISN VTKIPYEVL KGTNAINKD YK
    CDS sequence
    • >TC033306 RA
      atggttgca ctcactgct tgtttattt tttttgttt 
      gccactgag gcaatgtca atgttaaat ggagcccct 
      aaaccaaca tataaaaac gatattctg gctattaaa 
      cacatcaaa gacagtgta gtactgaaa tcatgtata 
      ggagtcgaa atttccact ttttgggta ctttcatca 
      gctctgtgc cttcacagc aatatttcg gacaaagta 
      gttgttgaa gctcgagtt aacgaggaa aaagtctta 
      cgttacacc aataccgag gttaaaatt catccagat 
      tatgataaa atgagcggg aacaatgac atcggtgtg 
      cttcatatc aaaggaggg aaacatatt gaagggatg 
      ggtcaacac cttatactt ccatttgct cattttcct 
      aaaatgaat ggaagtata agtgcttgg aatttaaaa 
      tcaggcggt tattcccag atagttgag tctctagaa 
      gctggtcat ttaaaagtt aatatttat cttatgact 
      gattgcaaa ctgcaattt cctcagttg gacgcgcaa 
      gcccattta catggagac tatcagatt tgtacattt 
      ccaatggtt gaaggttca gatttgtgt gaaccagat 
      tccggggcg ccattgtac agttaccat aagtttagt 
      aatagatgg ggattgatt gggatttac aatgatatt 
      atggctgca acaacagga cgtacacat cagtgtaat 
      caagaacat cgacatccg ttgattttt acacagatt 
      tttcactac tttgggtgg atttctaat gttactaaa 
      attccctac gaagttttg aaaggaact aacgcaata 
      aataaagat tacaagtaa
    mRNA sequence
    • >TC033306 RA
      ataaaagaa tttaaacat tttttattt aaattagaa 
      gttgtgcaa agatggttg cactcactg cttgtttat 
      tttttttgt ttgccactg aggcaatgt caatgttaa 
      atggagccc ctaaaccaa catataaaa acgatattc 
      tggctatta aacacatca aagacagtg tagtactga 
      aatcatgta taggagtcg aaatttcca ctttttggg 
      tactttcat cagctctgt gccttcaca gcaatattt 
      cggacaaag tagttgttg aagctcgag ttaacgagg 
      aaaaagtct tacgttaca ccaataccg aggttaaaa 
      ttcatccag attatgata aaatgagcg ggaacaatg 
      acatcggtg tgcttcata tcaaaggag ggaaacata 
      ttgaaggga tgggtcaac accttatac ttccatttg 
      ctcattttc ctaaaatga atggaagta taagtgctt 
      ggaatttaa aatcaggcg gttattccc agatagttg 
      agtctctag aagctggtc atttaaaag ttaatattt 
      atcttatga ctgattgca aactgcaat ttcctcagt 
      tggacgcgc aagcccatt tacatggag actatcaga 
      tttgtacat ttccaatgg ttgaaggtt cagatttgt 
      gtgaaccag attccgggg cgccattgt acagttacc 
      ataagttta gtaatagat ggggattga ttgggattt 
      acaatgata ttatggctg caacaacag gacgtacac 
      atcagtgta atcaagaac atcgacatc cgttgattt 
      ttacacaga tttttcact actttgggt ggatttcta 
      atgttacta aaattccct acgaagttt tgaaaggaa 
      ctaacgcaa taaataaag attacaagt aaaaaatgt 
      atattaatc gaatttcaa ataaatatt tttctatgt 
      t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY