Status
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Gene information
General information
TC030892 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 136026..138780
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030892
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10148

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030892 RA
      MICLILLIV LFVSGGFTL ECNTINHRQ 
      KFKPDIILA QHYCKNVPS NLVIDSLYF 
      VETNYPQEV TIVDSEIPT ISNYSSLLN 
      MKYVRKLVL KGLGIRNII PGAFRNMNN 
      LQELDLSFN QIRELSGDA FQGLERLET 
      LHLEGNQLR DDLEEKRKF LPNLKNLFL 
      SDNPIGAAE
    CDS sequence
    • >TC030892 RA
      atgatttgt ttaattttg ttgattgtg ttgttcgtt 
      tctgggggt tttaccctt gagtgtaat acgataaac 
      caccgccaa aaattcaag cctgatatt attttggca 
      caacattac tgtaaaaat gttccgtcg aatttggtc 
      attgatagt ttgtatttc gttgaaaca aattatccg 
      caagaagtg actatcgtt gattcggaa ataccaaca 
      atttccaat tattcgtcg cttcttaac atgaaatat 
      gtgcgaaag ttggttttg aaaggcttg gggattagg 
      aatataatc cctggtgcg tttcgcaac atgaacaat 
      ttgcaagaa ttggatttg agttttaac cagatcagg 
      gaattgtcc ggtgatgcg tttcaggga ttagaaagg 
      ttggagacg ttgcatttg gagggaaat cagttaagg 
      gatgatttg gaggagaag agaaagttt ttgcccaat 
      ttgaagaat ttgttcctg agtgataat ccgatcgga 
      gcggctgaa taa
    mRNA sequence
    • >TC030892 RA
      ttcaaaagg cgcattttt tatcaagat gaaagtcca 
      atttctcga ctcgtttac attggaact gacttatcg 
      tattttatt tctaatgtt taccaaatt ttggcttac 
      aatttttgg aagaaaaag tactggagt gaatttttg 
      gccaatttg aaactgtta aaaggacga aaaatacgt 
      aaaaatctc tactatttg ttgcttttc gtcataaat 
      gtgctctat gttttaact ctttgttat gccgtttat 
      tactggaga caacaatat gaagatttt tggcatcgc 
      tatacttta agtttcatc gagaattat tgtcaattt 
      ttgtataat tgtttccac tccacttta ttattaatg 
      attttgtca cgatatcaa cgtttgaaa gcacaatta 
      tggcaaaat cgatacgaa acggtcgct catggcatg 
      accgttcag aaaacaacg ctatttttt ttcaatcga 
      acttttggc gtccccatt tttctagtt cttgtgttt 
      gttcaaatg caatctgtg caagatttg agcgatatt 
      ttctactac aaagataca gctcagctt gtctctctt 
      gcactttta atgcaatta actcaacta gttccggcc 
      ttgtttgtg atttatttg tgtggacgg gtgatggaa 
      gaacagaac aaaattcgc ttaattacc tttgaaatg 
      aacaagagc ttcgaacgt gataagaac aagttgggg 
      gattttgtt gctttgata agggatgct caagttaag 
      ataacagct gctgatttt tttattttg gataaatcg 
      actattttg aaagttttg gacacggtt gtggcgttt 
      ttgatggtg gttgcgcaa tttcaggat taaataaag 
      atttttttc ttctttgaa ttgtgtttt tttgcaaaa 
      aattagaag ccgcttgaa aacacaaat taattattt 
      tggtcattt tttgtcgaa atttttcta aatcactga 
      atttcaagt ttagaataa cacaagatt aatgcattt 
      ttcaatcaa caagcgcac atttctatg attaatatt 
      attcttcta caaacaaat atttgaaca attccataa 
      ttcgaacac aactgttta ttgtcgaaa tgagttccc 
      aactattga aactgactc acgacgccc tcaaaattt 
      taggaattg gcccaaaaa gtaacaaaa tttactcgt 
      tcctgttac tcacaactt tgactcttt tggtcattc 
      tgtccagca ttgatcgac cttatctca aatcctaca 
      cttacatca agctcgttg tgagtgttt tgatggact 
      ttattgctt acttcttca atttttgga cgattttgt 
      tcagtcgaa gcgattttt tccaacagt ttgagtgtg 
      acaatgagc cgaaactgc gccactatt taatcttta 
      tttttgtga atgtgtttt tttgggtga ttattttaa 
      tgtcgaatt atgccttca ctcgcatca ttctattga 
      aatctgtgc tcgaaatcg tgataattg acatcgaaa 
      tttacagcc aatttttat acggtttca tgatttatc 
      taattttgg acacgatca agtcaaagt atcgccaaa 
      tgcaccgac ttttggcca attacaagg tgataacgt 
      ccgatgagt ttttctatc tcgtttcaa aaatcgaaa 
      gtttggctt gtgagttaa aaaatacag tcgacgatt 
      ttaatgaca ttttcggga tttctttcc ttttaataa 
      tatcgtact cgactctac atttcgtca attacatcg 
      acgatttat tttttttcc ggttcgaaa aatcaaaat 
      ttgagccgt ttctcatct caaacattt cgctagttt 
      cgctaattt tcctcttta attgcactt taatcatta 
      tgtgtgatt gtgtgcgta gcgaagcgt caaaagtcg 
      tgaaaaatg cacaaaagt tgagacata aattcgatt 
      taaaaaccc ttgttttga attttccca agtttacag 
      ccggcggtt ttttccacg tgaaaaagt ccacgattt 
      ttagtattt tgaacacag ttagtaccc ttctaatcg 
      ttatggtcc agttcgata aagaacgat aagcgtgat 
      acactgacc cctgaccga aaaacacga atttgtttg 
      ccaataatt cagttccga tcaaataac tgtgatgat 
      ttgtttaat tttgttgat tgtgttgtt cgtttctgg 
      gggttttac ccttgagtg taatacgat aaaccaccg 
      ccaaaaatt caagcctga tattatttt ggcacaaca 
      ttactgtaa aaatgttcc gtcgaattt ggtcattga 
      tagtttgta tttcgttga aacaaatta tccgcaaga 
      agtgactat cgttgattc ggaaatacc aacaatttc 
      caattattc gtcgcttct taacatgaa atatgtgcg 
      aaagttggt tttgaaagg cttggggat taggaatat 
      aatccctgg tgcgtttcg caacatgaa caatttgca 
      agaattgga tttgagttt taaccagat cagggaatt 
      gtccggtga tgcgtttca gggattaga aaggttgga 
      gacgttgca tttggaggg aaatcagtt aagggatga 
      tttggagga gaagagaaa gtttttgcc caatttgaa 
      gaatttgtt cctgagtga taatccgat cggagcggc 
      tgaataatt aatcaagta gtgaaataa aaataaact 
      gaaaacaaa act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY