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Gene information
General information
TC014816 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15437258..15441292
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014816
    iBeetle screen iB_10141

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014816 RA
      MITQEVDFE HCGERESET FNNLLLYDR DVCCKMDRI 
      LKGIMRYRN VKKEKMVKQ FKEVKDNPM PKAVFFTCI 
      DSRMIPTRF TQTNVGDMF VVRNAGNII PHSQHFLDE 
      LTTNEPAAL ELGCVVNDI RHIIVCGHS DCKAINLLY 
      KLQDSEFAS QDNRRISPL RAWLCTHAL TSLEKFQQL 
      QVTDYGKPL IFQAETPMR KFVAYIDPE NKFTIEDKL 
      SQINTLQQL QNIASYGFL KKRLEKHQL HIHALWFDI 
      YTGEIYYFS RGAKKFVVI DEENFPKLL QEVEKYYS
    CDS sequence
    • >TC014816 RA
      atgatcact caagaggtg gactttgag cattgtggg 
      gagagggaa agcgagacc ttcaacaac ttgttgctt 
      tacgatcgt gatgtttgt tgtaaaatg gatcggatt 
      ctcaaaggt ataatgagg tacagaaat gtcaagaag 
      gaaaaaatg gtcaagcag ttcaaagaa gtcaaagac 
      aatcctatg ccgaaagct gttttcttt acatgtatt 
      gacagtaga atgataccg acaaggttt acacaaact 
      aatgtgggc gatatgttt gtagtgcgt aatgctggc 
      aacatcatc ccgcattcc caacacttt ttggacgaa 
      ctgactacc aatgaaccc gcagccctc gaactgggc 
      tgtgtagtg aacgacatc cgccacatc attgtttgt 
      ggtcacagt gactgcaaa gccatcaat ctcttatac 
      aaactacaa gacagtgaa tttgcttct caggataat 
      cgaagaata tcacccctt agagcctgg ctatgtaca 
      catgctctc acaagttta gaaaaattt caacaactg 
      caagtgaca gactacggc aaacctcta atttttcaa 
      gccgaaacc cctatgaga aaattcgtt gcttacatc 
      gatcccgaa aacaaattt acaatagaa gataagcta 
      tcgcaaatc aatactttg caacagtta caaaatatc 
      gcaagttat ggcttttta aaaaagaga ttggagaaa 
      catcaactg catattcat gccctttgg tttgatatt 
      tacacaggc gagatttat tattttagt cggggggcg 
      aaaaaattt gtggtcatt gatgaagag aatttcccc 
      aaattgctc caagaggtg gaaaaatat tattcgtga
    mRNA sequence
    • >TC014816 RA
      tcggacgtg cattttttc gtcaaaatt aatttgaat 
      cgatattga tgatcactc aagaggtgg actttgagc 
      attgtgggg agagggaaa gcgagacct tcaacaact 
      tgttgcttt acgatcgtg atgtttgtt gtaaaatgg 
      atcggattc tcaaaggta taatgaggt acagaaatg 
      tcaagaagg aaaaaatgg tcaagcagt tcaaagaag 
      tcaaagaca atcctatgc cgaaagctg ttttcttta 
      catgtattg acagtagaa tgataccga caaggttta 
      cacaaacta atgtgggcg atatgtttg tagtgcgta 
      atgctggca acatcatcc cgcattccc aacactttt 
      tggacgaac tgactacca atgaacccg cagccctcg 
      aactgggct gtgtagtga acgacatcc gccacatca 
      ttgtttgtg gtcacagtg actgcaaag ccatcaatc 
      tcttataca aactacaag acagtgaat ttgcttctc 
      aggataatc gaagaatat cacccctta gagcctggc 
      tatgtacac atgctctca caagtttag aaaaatttc 
      aacaactgc aagtgacag actacggca aacctctaa 
      tttttcaag ccgaaaccc ctatgagaa aattcgttg 
      cttacatcg atcccgaaa acaaattta caatagaag 
      ataagctat cgcaaatca atactttgc aacagttac 
      aaaatatcg caagttatg gctttttaa aaaagagat 
      tggagaaac atcaactgc atattcatg ccctttggt 
      ttgatattt acacaggcg agatttatt attttagtc 
      ggggggcga aaaaatttg tggtcattg atgaagaga 
      atttcccca aattgctcc aagaggtgg aaaaatatt 
      attcgtgaa atgattaag actgcgttt tttgttgtt 
      gtgttaatc aattaattt atttttata tatttttct 
      aataagaaa taaagttta ataaatgtg gttcattta 
      taaacacag agtttaggc aacacataa tatagatgc 
      ggttcgttg ctatttttt tttaatttt caagtattt 
      tttattaca aaatgcaaa tgtgtcgtg cttaaacta 
      ttacatacc attaggccg ttttgaagt gatgagatg 
      tcgctacca gtgcactac aattcacca ccaccacca 
      cctatagaa aaataagga aaataatga aaagaaata 
      aaagaaaat gttacggaa ctgttacct tcatattgt 
      atttgtaac aaattatca taaatatat tcgttatag 
      gggaaataa ttgtataat aatattgct acacaatct 
      taatgaaaa gaaggaaaa aaatataac aagtgtgaa 
      acaaatcgt aacaataag gaaaaatga gtgaataat 
      gattaatgt caatgatat gcacttacc gaggatggg 
      ccaagcaaa gaaacttac aatcataat tgatgtttc 
      ttttattga tgtagctat caacagagg atgcccact 
      gttcggatc acacaatca atcaatatt ttacaatca 
      caatgcact cagtgcatc agtctaata tattaggca 
      cagtgtaca aaaagacaa cgaaaacca aatgctatg 
      ttcatctta ttacaaaga aatggtgag aaatgtggc 
      tggttttgt atatataaa gtagtagca cctacaaaa 
      acacaatat tgtccctaa atatacatt taatcaata 
      tttacaaaa tctggtgag atgaacgca ttcgagatt 
      tctaagtct aaacatagc atttgcggg ttttccagc 
      ctcgcttta aactattca tttttttaa caaatatct 
      taataaaaa ctgcacata gttcatttc ctacaagat 
      tattacaaa attgaaaaa catcagtaa aactgtaac 
      atatacaaa caaacttgt cacactgtc cgtcatgaa 
      acgaaactt tagccaatt tgtttattt gtcctgtta 
      tcactttca cagcttcag agtgttcgg atagttggt 
      tttaaaatt tcttcttcg tggatattc ggcacgact 
      cgcagctga ttttcaaaa ttagtccgg gataaatca 
      acactttta attaattca actacctcg caacgttct 
      tctattata tattacaat ttaatttat taaaaagtc 
      cactcgaca gtaaaatca aatgagcac tgcactact 
      atactttta tattttctt tttgattgc ttttcagcc 
      cgatccgaa gagtggggg ccactagtt tgttattct 
      tttatttat acaactata ttactagaa tccatatac 
      ctttttagt taaagtcga tatttaaaa attcaattc 
      aaacccaca tgaagaaat taattggaa attttttta 
      tggattttt ttggttttt ctttaaaat agcaacacc 
      acatagacc cagatgatt cactgaaac ttttttctt 
      aggcaaata cacatcaat taactcaac cggtcgtta 
      ttatctcat tgaaagcaa catgatata agcaaatta 
      ttatcgtaa caactgtat gtagaaaaa ttaactggg 
      aggggaggg ggcgtctaa actagagca atgcagaaa 
      ccatcagta cggattaac taatctgaa taccgtcta 
      tgtgaagta tatgaaata acaagctag taatagatt 
      ataacacta cagtaaaga gcagccata acaatgtgt 
      tattttttt ctttattct tttatatta tcatcgatt 
      ttttttttt gtttaattg cgtccgcct cggcctcca 
      cgaccccga ttgttgtcg ccgcggtcc tgcaacaaa 
      acaaacata aaataccta acatgaaaa aatattata 
      aaaaaacac attttatta ttttaccac ttcaaaatc 
      gcgcatttt aacttaact aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY