Status
Please wait ...
Gene information
General information
TC033276
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012994
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15426347..15428306
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033276
    iBeetle screen iB_10140

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033276 RA
      MDKKYEKHC RICVKETTN GVYLFDATI SDSETYIVD 
      FLRQITNLE ISEDDGLTN ILCPTCVTK VFDCFDLVQ 
      ICIASDAIL RSAKDTKCD SNKQNSDLI KSEDLNLPK 
      DSEFQCRIC KLTFSNKTS LNKHNKSTH KDDNPFKCD 
      QCSQSFRKK MHLNVHQRS HTKDEDKKF FCKSCDKQF 
      MYEYLLRQH EYKHTDEKP FPCSVCNRG CLTAESLRR 
      HMRIHDRNY VKKKHSCEI CHKEFPYPS FLAEHMKNH 
      TGEKPHLCS TCGKGFRQS GALHYHQRI HTGDKKFSC 
      TICNGRFMS QSVLKVHMR KHTNERPYV CHICGMAFR 
      QSTDMKSHQ RTHSGDKPV LCNICGKRM STTGQLTIH 
      IRSHTGEKP FSCSNCPKA FTSRAMLVK HERIHTGER 
      PYRCNVCGK AFNQSGTLR THKKTHECD KKRRKSEKQ 
      KEVIEDKAY QSSDAVFFK NEFVNLETE IVTDEFIGS 
      NVQDIEVKT SDGCMKTQL TLILPAPIP LLQNL
    CDS sequence
    • >TC033276 RA
      atggacaag aaatatgag aaacactgc cgaatctgt 
      gtaaaagag acaacaaac ggtgtgtat ttatttgac 
      gcaacaatt tctgacagt gagacctac attgttgat 
      tttttacgc caaataacg aatttggag ataagtgaa 
      gacgatggc cttacgaat attttatgt cctacatgt 
      gttaccaaa gtttttgac tgttttgat ttggtccaa 
      atatgtatt gcttcagat gctattctt cggagtgct 
      aaggacacg aaatgtgat tcaaataag caaaatagt 
      gatttaatc aaatcagaa gacctaaat ttgcctaaa 
      gatagtgaa tttcaatgt aggatttgt aaattgact 
      ttcagcaat aaaactagc ctgaacaaa cataataaa 
      agtacacac aaggacgac aatcctttt aaatgtgat 
      cagtgttca caatcattt cggaaaaag atgcacttg 
      aatgtacat caacgctca cataccaaa gacgaggat 
      aagaagttt ttttgtaaa agttgtgac aaacaattc 
      atgtatgaa tatttactg agacagcat gagtacaaa 
      cacacggat gagaaacct tttccctgc agtgtctgt 
      aatagaggg tgccttact gcggaaagc ttaagacgc 
      cacatgcga atccacgat cgaaattac gtaaaaaag 
      aagcactcg tgtgaaatt tgccacaaa gaattcccc 
      taccctagt tttctagct gaacatatg aaaaatcac 
      accggggaa aaacctcac ttgtgctcc acttgtggt 
      aagggtttt cgacaaagt ggagcctta cattaccat 
      cagaggatt cacactggt gataaaaaa ttcagttgt 
      acgatttgt aatggacgt ttcatgtct caaagtgtt 
      ctaaaagtg cacatgcga aagcacaca aatgaaagg 
      ccttacgtt tgccatatc tgcggtatg gcgtttaga 
      caatcaacg gacatgaaa agtcaccaa agaacccac 
      agtggtgac aaaccagta ctgtgtaat atttgtggc 
      aagcggatg tcaacgacc ggccaacta accattcat 
      atcagatct cacaccggc gaaaagccg ttttcttgc 
      tcaaattgc cctaaagcg ttcacgtct agggcgatg 
      ttagtcaaa cacgaacgg attcataca ggagagagg 
      ccgtatcga tgtaatgtt tgtggcaaa gcgttcaat 
      caaagcggg actttaagg acgcacaaa aaaactcat 
      gaatgtgat aagaaacgg agaaaaagt gaaaaacaa 
      aaagaagtt atcgaggat aaggcctat caaagttcg 
      gacgctgtg tttttcaaa aacgaattt gtcaatttg 
      gaaactgag attgttact gacgagttt attggtagt 
      aacgtccag gatattgaa gttaagacg agtgatggg 
      tgtatgaaa actcaactg acgcttatt ctgcctgcg 
      cctataccg ctactgcaa aatctgtaa
    mRNA sequence
    • >TC033276 RA
      tgctatttt ttacctgga cggaagcac gtaacacca 
      atttattgt ccaagaaat ggacaagaa atatgagaa 
      acactgccg aatctgtgt aaaagagac aacaaacgg 
      tgtgtattt atttgacgc aacaatttc tgacagtga 
      gacctacat tgttgattt tttacgcca aataacgaa 
      tttggagat aagtgaaga cgatggcct tacgaatat 
      tttatgtcc tacatgtgt taccaaagt ttttgactg 
      ttttgattt ggtccaaat atgtattgc ttcagatgc 
      tattcttcg gagtgctaa ggacacgaa atgtgattc 
      aaataagca aaatagtga tttaatcaa atcagaaga 
      cctaaattt gcctaaaga tagtgaatt tcaatgtag 
      gatttgtaa attgacttt cagcaataa aactagcct 
      gaacaaaca taataaaag tacacacaa ggacgacaa 
      tccttttaa atgtgatca gtgttcaca atcatttcg 
      gaaaaagat gcacttgaa tgtacatca acgctcaca 
      taccaaaga cgaggataa gaagttttt ttgtaaaag 
      ttgtgacaa acaattcat gtatgaata tttactgag 
      acagcatga gtacaaaca cacggatga gaaaccttt 
      tccctgcag tgtctgtaa tagagggtg ccttactgc 
      ggaaagctt aagacgcca catgcgaat ccacgatcg 
      aaattacgt aaaaaagaa gcactcgtg tgaaatttg 
      ccacaaaga attccccta ccctagttt tctagctga 
      acatatgaa aaatcacac cggggaaaa acctcactt 
      gtgctccac ttgtggtaa gggttttcg acaaagtgg 
      agccttaca ttaccatca gaggattca cactggtga 
      taaaaaatt cagttgtac gatttgtaa tggacgttt 
      catgtctca aagtgttct aaaagtgca catgcgaaa 
      gcacacaaa tgaaaggcc ttacgtttg ccatatctg 
      cggtatggc gtttagaca atcaacgga catgaaaag 
      tcaccaaag aacccacag tggtgacaa accagtact 
      gtgtaatat ttgtggcaa gcggatgtc aacgaccgg 
      ccaactaac cattcatat cagatctca caccggcga 
      aaagccgtt ttcttgctc aaattgccc taaagcgtt 
      cacgtctag ggcgatgtt agtcaaaca cgaacggat 
      tcatacagg agagaggcc gtatcgatg taatgtttg 
      tggcaaagc gttcaatca aagcgggac tttaaggac 
      gcacaaaaa aactcatga atgtgataa gaaacggag 
      aaaaagtga aaaacaaaa agaagttat cgaggataa 
      ggcctatca aagttcgga cgctgtgtt tttcaaaaa 
      cgaatttgt caatttgga aactgagat tgttactga 
      cgagtttat tggtagtaa cgtccagga tattgaagt 
      taagacgag tgatgggtg tatgaaaac tcaactgac 
      gcttattct gcctgcgcc tataccgct actgcaaaa 
      tctgtaata gtaaaaaaa taaacgcta tttttctac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY