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Gene information
General information
TC014808 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15352188..15353955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014808
    iBeetle screen iB_10138

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014808 RA
      MVVKVYISG ISGNKEVKK RQQRVLLIL 
      DSKNINYEV IDIAEPENE EKKDFMQNN 
      STSSGATIS DPNPRHPLP PQIFNDDVY 
      CGDYDQFDM ANEVDEMEK FLKLAPNDS 
      EPSVTTNAE LKLGNGEVH NASQELNET 
      KETEESKEE TDNNEKIEN GEETEEKHD 
      EENEEEADE
    CDS sequence
    • >TC014808 RA
      atggtagtc aaagtttac attagcggc atttccggc 
      aataaagag gtgaagaaa agacagcaa agggtcctt 
      ctgatttta gattcaaaa aatatcaat tatgaagtt 
      atagatatt gccgaaccc gaaaatgag gagaaaaag 
      gatttcatg cagaacaat tccacgtct tcaggggca 
      acaatcagt gaccctaat ccaaggcat ccactacct 
      ccacagata ttcaacgac gatgtctat tgtggggac 
      tatgaccaa ttcgatatg gcaaacgaa gtggacgag 
      atggaaaaa tttctaaaa ctagcaccg aacgattct 
      gaaccctct gtaacaaca aatgctgaa ctaaaactg 
      ggcaatggt gaagttcac aacgcctct caagaatta 
      aatgaaacc aaagaaaca gaagaatca aaagaggaa 
      actgacaat aacgaaaaa atcgaaaat ggcgaagaa 
      actgaagaa aaacatgac gaagaaaat gaggaagag 
      gctgatgaa taa
    mRNA sequence
    • >TC014808 RA
      tcagtttat taaaaagtc gtgacaaat tttgcgtga 
      gtgcacatg actcaccgt tgggtttat gagtttttc 
      tgccgttag tttctcacc agcccgtta taacagaat 
      ttagcaaca aataaattg caaataatt tgttagtgc 
      attctaaaa ttagcctcc agtttttga attggttcg 
      tttctcggc tgaccctta atgacaaca atcgacaat 
      caacctgct gtaccaaaa taggtgtgg cgccacact 
      gccaaagaa gctgtcaaa taggtttag agaaaattt 
      acaattttt acaatataa actgagtaa taatggtag 
      tcaaagttt acattagcg gcatttccg gcaataaag 
      aggtgaaga aaagacagc aaagggtcc ttctgattt 
      tagattcaa aaaatatca attatgaag ttatagata 
      ttgccgaac ccgaaaatg aggagaaaa aggatttca 
      tgcagaaca attccacgt cttcagggg caacaatca 
      gtgacccta atccaaggc atccactac ctccacaga 
      tattcaacg acgatgtct attgtgggg actatgacc 
      aattcgata tggcaaacg aagtggacg agatggaaa 
      aatttctaa aactagcac cgaacgatt ctgaaccct 
      ctgtaacaa caaatgctg aactaaaac tgggcaatg 
      gtgaagttc acaacgcct ctcaagaat taaatgaaa 
      ccaaagaaa cagaagaat caaaagagg aaactgaca 
      ataacgaaa aaatcgaaa atggcgaag aaactgaag 
      aaaaacatg acgaagaaa atgaggaag aggctgatg 
      aataaagaa aaaagaaaa caacaccaa gatcgtatt 
      tattgttta ttatttgtc ttgaatgac ttttttaag 
      tattatatt tacgctatt attcatgag gttaaggtc 
      ctaagtagc tcacggcga acaaattgc atactattg 
      ttacacttt tacatttgt aatgttgaa ccgatctga 
      attttgtaa atatattac ttcatctat taagttgcc 
      caatatatt cataaactt tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY